SYVN1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SYVN1
Gene Name:  synovial apoptosis inhibitor 1, synoviolin
Original Clone ID:
FLH262812.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
HsCD00080091
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1854nts
Open reading frame: 1 to 1854

ATGTTCCGCACGGCAGTGATGATGGCGGCCAGCCTGGCGCTGACCGGGGCTGTGGTGGCT
CACGCCTACTACCTCAAACACCAGTTCTACCCCACTGTGGTGTACCTGACCAAGTCCAGC
CCCAGCATGGCAGTCCTGTACATCCAGGCCTTTGTCCTTGTCTTCCTTCTGGGCAAGGTG
ATGGGCAAGGTGTTCTTTGGGCAACTGAGGGCAGCAGAGATGGAGCACCTTCTGGAACGT
TCCTGGTACGCCGTCACAGAGACTTGTCTGGCCTTCACCGTTTTTCGGGATGACTTCAGC
CCCCGCTTTGTTGCACTCTTCACTCTTCTTCTCTTCCTCAAATGTTTCCACTGGCTGGCT
GAGGACCGTGTGGACTTTATGGAACGCAGCCCCAACATCTCCTGGCTCTTTCACTGCCGC
ATTGTCTCTCTTATGTTCCTCCTGGGCATCCTGGACTTCCTCTTCGTCAGCCACGCCTAT
CACAGCATCCTGACCCGTGGGGCCTCTGTGCAGCTGGTGTTTGGCTTTGAGTATGCCATC
CTGATGACGATGGTGCTCACCATCTTCATCAAGTATGTGCTGCACTCCGTGGACCTCCAG
AGTGAGAACCCCTGGGACAACAAGGCTGTGTACATGCTCTACACAGAGCTGTTTACAGGC
TTCATCAAGGTTCTGCTGTACATGGCCTTCATGACCATCATGATCAAGGTGCACACCTTC
CCACTCTTTGCCATCCGGCCCATGTACCTGGCCATGAGACAGTTCAAGAAAGCTGTGACA
GATGCCATCATGTCTCGCCGAGCCATCCGCAACATGAACACCCTGTATCCAGATGCCACC
CCAGAGGAGCTCCAGGCAATGGACAATGTCTGCATCATCTGCCGAGAAGAGATGGTGACT
GGTGCCAAGAGACTGCCCTGCAACCACATTTTCCATACCAGCTGCCTGCGCTCCTGGTTC
CAGCGGCAGCAGACCTGCCCCACCTGCCGTATGGATGTCCTTCGTGCATCGCTGCCAGCG
CAGTCACCACCACCCCCGGAGCCTGCGGATCAGGGGCCACCCCCTGCCCCCCACCCCCCA
CCACTCTTGCCTCAGCCCCCCAACTTCCCCCAGGGCCTCCTGCCTCCTTTTCCTCCAGGC
ATGTTCCCACTGTGGCCCCCCATGGGCCCCTTTCCACCTGTCCCGCCTCCCCCCAGCTCA
GGAGAGGCTGTGGCTCCTCCATCCACCAGTGCAGCAGCCCTTTCTCGGCCCAGTGGAGCA
GCTACAACCACAGCTGCTGGCACCAGTGCTACTGCTGCTTCTGCCACAGCATCTGGCCCA
GGCTCTGGCTCTGCCCCAGAGGCTGGCCCTGCCCCTGGTTTCCCCTTCCCTCCTCCCTGG
ATGGGTATGCCCCTGCCTCCACCCTTTGCCTTCCCCCCAATGCCTGTGCCCCCTGCGGGC
TTTGCTGGGCTGACCCCAGAGGAGCTACGAGCTCTGGAGGGCCATGAGCGGCAGCACCTG
GAGGCCCGGCTGCAGAGCCTGCGTAACATCCACACACTGCTGGACGCCGCCATGCTGCAG
ATCAACCAGTACCTCACCGTGCTGGCCTCCTTGGGGCCCCCCCGGCCTGCCACTTCAGTC
AACTCCACTGAGGAGACTGCCACTACAGTTGTTGCTGCTGCCTCCTCCACCAGCATCCCT
AGCTCAGAGGCCACGACCCCAACCCCAGGAGCCTCCCCACCAGCCCCTGAAATGGAAAGG
CCTCCAGCTCCTGAGTCAGTGGGCACAGAGGAGATGCCTGAGGATGGAGAGCCCGATGCA
GCAGAGCTCCGCCGGCGCCGCCTGCAGAGGCTGGAGTCTCCTGTTGCCCACTTG

Protein sequence predicted based on nucleotide sequence

MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKV
MGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA
EDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSILTRGASVQLVFGFEYAI
LMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHTF
PLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMVT
GAKRLPCNHIFHTSCLRSWFQRQQTCPTCRMDVLRASLPAQSPPPPEPADQGPPPAPHPP
PLLPQPPNFPQGLLPPFPPGMFPLWPPMGPFPPVPPPPSSGEAVAPPSTSAAALSRPSGA
ATTTAAGTSATAASATASGPGSGSAPEAGPAPGFPFPPPWMGMPLPPPFAFPPMPVPPAG
FAGLTPEELRALEGHERQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSV
NSTEETATTVVAAASSTSIPSSEATTPTPGASPPAPEMERPPAPESVGTEEMPEDGEPDA
AELRRRRLQRLESPVAHL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1889
Start on reference sequence 36
Mutations:
None
Discrepancy:
a1829g,K610R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable