Gene:
Gene Symbol:
CHD4
Gene Name:
chromodomain helicase DNA binding protein 4
Original Clone ID:
FLH262823.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
HIP
Description:
None
Comments:
plate source, forward and reverse reads, produced 0 bp after trim. Tube source, forward and reverse reads, failed to contig with refseq. BLAST returned HsCD00079949 (SMARCAD1)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00080095
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGTCGGGCCTGGGCTCCCCGTCCCCCTGCTCGGCGGGCAGTGAGGAGGAGGATATG
GATGCACTTTTGAACAACAGCCTGCCCCCACCCCACCCAGAGGACCCAGAAGAGGATTTG
TCAGAAACAGAGACTCCAAAGCTCAAGAAGAAGAAAAAGCCTAAGAAACCTCGGGACCCT
AAAATCCCTAAGAGCAAGCGCCAAAAAAAGGAGCGTATGCTCTTATGCCGGCAGCTGGGG
GACAGCTCTGGGGAGGGGCCAGAGTTTGTGGAGGAGGAGGAAGAGGTGGCTCTGCGCTCA
GACAGTGAGGGCAGCGACTATACTCCTGGCAAGAAGAAGAAGAAGAAGCTTGGACCTAAG
AAAGAGAAGAAGAGCAAATCCAAGCGGAAGGAGGAGGAGGAGGAGGAGGATGATGATGAT
GATTCAAAGGAGCCTAAATCATCTGCTCAGCTCCTGGAAGACTGGGGCATGGAAGACATT
GACCACGTGTTCTCAGAGGAGGATTATCGAACCCTCACCAACTACAAGGCCTTCAGCCAG
TTTGTCAGACCCCTCATTGCTGCCAAAAATCCCAAGATTGCTGTCTCCAAGATGATGATG
GTTTTGGGTGCAAAATGGCGGGAGTTCAGTACCAATAACCCCTTCAAAGGCAGTTCTGGG
GCATCAGTGGCAGCTGCGGCAGCAGCAGCGGTAGCTGTGGTGGAGAGCATGGTGACAGCC
ACTGAGGTTGCACCACCACCTCCCCCTGTGGAGGTGCCTATCCGCAAGGCCAAGACCAAG
GAGGGCAAAGGTCCCAATGCTCGGAGGAAGCCCAAGGGCAGCCCTCGTGTACCTGATGCC
AAGAAGCCTAAACCCAAGAAAGTAGCTCCCCTGAAAATCAAGCTGGGAGGTTTTGGTTCC
AAGCGTAAGAGATCCTCGAGTGAGGATGATGACTTAGATGTGGAATCTGACTTCGATGAT
GCCAGTATCAATAGCTATTCTGTTTCTGATGGTTCCACCAGCCGTAGTAGCCGCAGCCGC
AAGAAACTCCGAACCACTAAAAAGAAAAAGAAAGGCGAGGAGGAGGTGACTGCTGTGGAT
GGTTATGAGACAGACCACCAGGACTATTGCGAGGTGTGCCAGCAAGGCGGTGAGATCATC
CTGTGTGATACCTGTCCCCGTGCTTACCACATGGTCTGCCTGGATCCCGACATGGAGAAG
GCTCCCGAGGGCAAGTGGAGCTGCCCACACTGCGAGAAGGAAGGCATCCAGTGGGAAGCT
AAAGAGGACAATTCGGAGGGTGAGGAGATCCTGGAAGAGGTTGGGGGAGACCTCGAAGAG
GAGGATGACCACCATATGGAATTCTGTCGGGTCTGCAAGGATGGTGGGGAACTGCTCTGC
TGTGATACCTGTCCTTCTTCCTACCACATCCACTGCCTGAATCCCCCACTTCCAGAGATC
CCCAACGGTGAATGGCTCTGTCCCCGTTGTACGTGTCCAGCTCTGAAGGGCAAAGTGCAG
AAGATCCTAATCTGGAAGTGGGGTCAGCCACCATCTCCCACACCAGTGCCTCGGCCTCCA
GATGCTGATCCCAACACGCCCTCCCCAAAGCCCTTGGAGGGGCGGCCAGAGCGGCAGTTC
TTTGTGAAATGGCAAGGCATGTCTTACTGGCACTGCTCCTGGGTTTCTGAACTGCAGCTG
GAGCTGCACTGTCAGGTGATGTTCCGAAACTATCAGCGGAAGAATGATATGGATGAGCCA
CCTTCTGGGGACTTTGGTGGTGATGAAGAGAAAAGCCGAAAGCGAAAGAACAAGGACCCT
AAATTTGCAGAGATGGAGGAACGCTTCTATCGCTATGGGATAAAACCCGAGTGGATGATG
ATCCACCGAATCCTCAACCACAGTGTGGACAAGAAGGGCCACGTCCACTACTTGATCAAG
TGGCGGGACTTACCTTACGATCAGGCTTCTTGGGAGAGTGAGGATGTGGAGATCCAGGAT
TACGACCTGTTCAAGCAGAGCTATTGGAATCACAGGGAGTTAATGAGGGGTGAGGAAGGC
CGACCAGGCAAGAAGCTCAAGAAGGTGAAGCTTCGGAAGTTGGAGAGGCCTCCAGAAACG
CCAACAGTTGATCCAACAGTGAAGTATGAGCGACAGCCAGAGTACCTGGATGCTACAGGT
GGAACCCTGCACCCCTATCAAATGGAGGGCCTGAATTGGTTGCGCTTCTCCTGGGCTCAG
GGCACTGACACCATCTTGGCTGATGAGATGGGCCTTGGGAAAACTGTACAGACAGCAGTC
TTCCTGTATTCCCTTTACAAGGAGGGTCATTCCAAAGGCCCCTTCCTAGTGAGCGCCCCT
CTTTCTACCATCATCAACTGGGAGCGGGAGTTTGAAATGTGGGCTCCAGACATGTATGTC
GTAACCTATGTGGGTGACAAGGACAGCCGTGCCATCATCCGAGAGAATGAGTTCTCCTTT
GAAGACAATGCCATTCGTGGTGGCAAGAAGGCCTCCCGCATGAAGAAAGAGGCATCTGTG
AAATTCCATGTGCTGCTGACATCCTATGAATTGATCACCATTGACATGGCTATTTTGGGC
TCTATTGATTGGGCCTGCCTCATCGTGGATGAAGCCCATCGGCTGAAGAACAATCAGTCT
AAGTTCTTCCGGGTATTGAATGGTTACTCACTCCAGCACAAGCTGTTGCTGACTGGGACA
CCATTACAAAACAATCTGGAAGAGTTGTTTCATCTGCTCAACTTTCTCACCCCCGAGAGG
TTCCACAATTTGGAAGGTTTTTTGGAGGAGTTTGCTGACATTGCCAAGGAGGACCAGATA
AAAAAACTGCATGACATGCTGGGGCCGCACATGTTGCGGCGGCTCAAAGCCGATGTGTTC
AAGAACATGCCCTCCAAGACAGAACTAATTGTGCGTGTGGAGCTGAGCCCTATGCAGAAG
AAATACTACAAGTACATCCTCACTCGAAATTTTGAAGCACTCAATGCCCGAGGTGGTGGC
AACCAGGTGTCTCTGCTGAATGTGGTGATGGATCTTAAGAAGTGCTGCAACCATCCATAC
CTCTTCCCTGTGGCTGCAATGGAAGCTCCTAAGATGCCTAATGGCATGTATGATGGCAGT
GCCCTAATCAGAGCATCTGGGAAATTATTGCTGCTGCAGAAAATGCTCAAGAACCTTAAG
GAGGGTGGGCATCGTGTACTCATCTTTTCCCAGATGACCAAGATGCTAGACCTGCTAGAG
GATTTCTTGGAACATGAAGGTTATAAATACGAACGCATCGATGGTGGAATCACTGGGAAC
ATGCGGCAAGAGGCCATTGACCGCTTCAATGCACCGGGTGCTCAGCAGTTCTGCTTCTTG
CTTTCCACTCGAGCTGGGGGCCTTGGAATCAATCTGGCCACTGCTGACACAGTTATTATC
TATGACTCTGACTGGAACCCCCATAATGACATTCAGGCCTTTAGCAGAGCTCACCGGATT
GGGCAAAATAAAAAGGTAATGATCTACCGGTTTGTGACCCGTGCGTCAGTGGAGGAGCGC
ATCACGCAGGTGGCAAAGAAGAAAATGATGCTGACGCATCTAGTGGTGCGGCCTGGGCTG
GGCTCCAAGACTGGATCTATGTCCAAACAGGAGCTTGATGATATCCTCAAATTTGGCACT
GAGGAACTATTCAAGGATGAAGCCACTGATGGAGGAGGAGACAACAAAGAGGGAGAAGAT
AGCAGTGTTATCCACTACGATGATAAGGCCATTGAACGGCTGCTAGACCGTAACCAGGAT
GAGACTGAAGACACAGAATTGCAGGGCATGAATGAATATTTGAGCTCATTCAAAGTGGCC
CAGTATGTGGTACGGGAAGAAGAAATGGGGGAGGAAGAGGAGGTAGAACGGGAAATCATT
AAACAGGAAGAAAGTGTGGATCCTGACTACTGGGAGAAATTGCTGCGGCACCATTATGAG
CAGCAGCAAGAAGATCTAGCCCGAAATCTGGGCAAAGGAAAAAGAATCCGTAAACAGGTC
AACTACAATGATGGCTCCCAGGAGGACCGAGGTGTGTGTGGCCGGCCCCGCCCCCCACCC
ATGGGCCGTTCCACTAGAGCAGTGGGCCCCGCTCATCTGCCCTCTCTCCCTCCAGATTGG
CAGGACGACCAGTCCGACAACCAGTCCGATTACTCAGTGGCTTCAGAGGAAGGTGATGAA
GACTTTGATGAACGTTCAGAAGCTCCCCGTAGGCCCAGTCGTAAGGGCCTGCGGAATGAT
AAAGATAAGCCATTGCCTCCTCTGTTGGCCCGTGTTGGTGGGAATATTGAAGTACTTGGT
TTTAATGCTCGTCAGCGAAAAGCCTTTCTTAATGCAATTATGCGATATGGTATGCCACCT
CAGGATGCTTTTACTACCCAGTGGCTTGTAAGAGACCTGCGAGGCAAATCAGAGAAAGAG
TTCAAGGCATATGTCTCTCTTTTCATGCGGCATTTATGTGAGCCGGGGGCAGATGGGGCT
GAGACCTTTGCTGATGGTGTCCCCCGAGAAGGCCTGTCTCGCCAGCATGTCCTTACTAGA
ATTGGTGTTATGTCTTTGATTCGCAAGAAGGTTCAGGAGTTTGAACATGTTAATGGGCGC
TGGAGCATGCCTGAACTGGCTGAGGTGGAGGAAAACAAGAAGATGTCCCAGCCAGGGTCA
CCCTCCCCAAAAACTCCTACACCCTCCACTCCAGGGGACACGCAGCCCAACACTCCTGCA
CCTGTCCCACCTGCTGAAGATGGGATAAAAATAGAGGAAAATAGCCTCAAAGAAGAAGAG
AGCATAGAAGGAGAAAAGGAGGTTAAATCTACAGCCCCTGAGACTGCCATTGAGTGTACA
CAGGCCCCTGCCCCTGCCTCAGAGGATGAAAAGGTCGTTGTTGAACCCCCTGAGGGAGAG
GAGAAAGTGGAAAAGGCAGAGGTGAAGGAGAGAACAGAGGAACCTATGGAGACAGAGCCC
AAAGGTGCTGCTGATGTAGAGAAGGTGGAGGAAAAGTCAGCAATAGATCTGACCCCTATT
GTGGTAGAAGACAAAGAAGAGAAGAAAGAAGAAGAAGAGAAAAAAGAGGTGATGCTTCAG
AATGGAGAGACCCCCAAGGACCTGAATGATGAGAAACAGAAGAAAAATATTAAACAACGT
TTCATGTTTAACATTGCAGATGGTGGTTTTACTGAGTTGCACTCCCTTTGGCAGAATGAA
GAGCGGGCAGCCACAGTTACCAAGAAGACTTATGAGATCTGGCATCGACGGCATGACTAC
TGGCTGCTAGCCGGCATTATAAACCATGGCTATGCCCGGTGGCAAGACATCCAGAATGAC
CCACGCTATGCCATCCTCAATGAGCCTTTCAAGGGTGAAATGAACCGTGGCAATTTCTTA
GAGATCAAGAATAAATTTCTAGCTCGAAGGTTTAAGCTCTTAGAACAAGCTCTGGTGATT
GAGGAACAGCTGCGCCGGGCTGCTTACTTGAACATGTCAGAAGACCCTTCTCACCCTTCC
ATGGCCCTCAACACCCGCTTTGCTGAGGTGGAGTGTTTGGCGGAAAGTCATCAGCACCTG
TCCAAGGAGTCAATGGCAGGAAACAAGCCAGCCAATGCAGTCCTGCACAAAGTTCTGAAA
CAGCTGGAAGAACTGCTGAGTGACATGAAAGCTGATGTGACTCGACTCCCAGCTACCATT
GCCCGAATTCCCCCAGTTGCTGTGAGGTTACAGATGTCAGAGCGTAACATTCTCAGCCGC
CTGGCAAACCGGGCACCCGAACCTACCCCACAGCAGGTAGCCCAGCAGCAGTTG
Protein sequence predicted based on nucleotide sequence
MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPEDPEEDLSETETPKLKKKKKPKKPRDP KIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPK KEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQ FVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTA TEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGS KRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVD GYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEA KEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEI PNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQF FVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDP KFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQD YDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATG GTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAP LSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASV KFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGT PLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVF KNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPY LFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLE DFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVII YDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGL GSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQD ETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYE QQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPMGRSTRAVGPAHLPSLPPDW QDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLG FNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGA ETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGS PSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECT QAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPI VVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNE ERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFL EIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHL SKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSR LANRAPEPTPQQVAQQQL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
163
End on reference sequence
5976
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
