Gene:
Gene Symbol:
CNTNAP5
Gene Name:
contactin associated protein-like 5
Original Clone ID:
FLH264823.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080204
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGATTCTTTACCACGGCTGACCAGCGTTTTGACTTTGCTGTTCTCTGGCTTGTGGCAT
TTAGGATTAACAGCGACAAACTACAACTGTGATGATCCACTAGCATCCCTGCTCTCTCCA
ATGGCTTTTTCCAGTTCCTCAGACCTCACTGGCACTCACAGCCCAGCTCAACTCAACTGG
AGAGTTGGAACTGGCGGTTGGTCCCCAGCAGATTCCAATGCTCAACAGTGGCTCCAGATG
GACCTGGGAAACAGAGTAGAGATTACAGCAGTGGCCACGCAGGGAAGATACGGAAGCTCT
GACTGGGTGACGAGTTACAGCCTGATGTTCAGTGACACAGGACGCAACTGGAAACAGTAC
AAACAAGAAGACAGCATCTGGACCTTTGCAGGAAACATGAATGCTGACAGCGTGGTGCAC
CACAAGCTATTGCACTCAGTGAGAGCCCGATTTGTTCGCTTTGTGCCCCTGGAATGGAAT
CCCAGTGGGAAGATTGGCATGAGAGTCGAGGTCTACGGATGTTCCTATAAATCAGATGTT
GCTGACTTTGATGGCCGAAGCTCACTTCTGTACAGGTTCAATCAGAAGTTGATGAGTACT
CTCAAAGATGTGATCTCCCTGAAGTTCAAGAGCATGCAAGGAGATGGGGTCCTGTTCCAT
GGAGAAGGTCAGCGTGGAGACCACATCACCTTGGAACTCCAGAAGGGGAGGCTCGCCCTA
CACCTCAATTTGGGTGACAGCAAAGCGCGGCTCAGCAGCAGCTTGCCCTCTGCCACCCTG
GGCAGCCTCCTGGATGACCAGCACTGGCACTCGGTCCTCATTGAGCGGGTGGGCAAGCAG
GTGAACTTCACGGTGGACAAGCACACACAGCACTTCCGCACCAAGGGCGAGACGGATGCC
TTAGACATTGACTATGAGCTTAGTTTTGGAGGAATTCCAGTACCAGGAAAACCTGGGACC
TTTTTAAAGAAAAACTTCCATGGATGCATCGAAAACCTTTACTACAATGGAGTAAACATA
ATTGACCTGGCTAAGAGACGAAAGCATCAGATCTATACTGGCAATGTCACTTTTTCCTGC
TCCGAACCACAGATTGTGCCCATCACATTTGTCAACTCCAGCGGCAGCTATTTGCTGCTG
CCCGGCACCCCCCAAATTGATGGGCTCTCAGTGAGTTTCCAGTTTCGAACATGGAACAAG
GATGGTCTGCTTCTGTCCACAGAGCTGTCTGAGGGCTCGGGAACCCTGCTGCTGAGCCTG
GAGGGTGGAATCCTGAGACTCGTGATTCAGAAAATGACAGAACGCGTAGCTGAAATCCTC
ACAGGCAGCAACTTGAATGATGGCCTGTGGCACTCGGTTAGCATCAACGCCAGGAGGAAC
CGCATCACGCTCACTCTGGATGATGAAGCAGCACCCCCGGCTCCAGACAGCACTTGGGTG
CAGATTTATTCTGGAAATAGCTACTATTTTGGAGGGTGCCCCGACAATCTCACCGATTCC
CAATGTTTAAATCCCATTAAGGCTTTCCAAGGCTGCATGAGGCTCATCTTTATTGATAAC
CAGCCCAAGGACCTCATTTCAGTTCAGCAAGGTTCCCTGGGGAATTTTAGTGATTTACAC
ATTGATCTGTGTAGCATCAAAGACAGGTGTTTGCCAAACTACTGTGAACATGGAGGAAGC
TGCTCCCAGTCCTGGACTACCTTCTATTGTAACTGCAGTGACACAAGTTACACTGGTGCC
ACCTGCCACAACTCCATCTACGAGCAATCCTGCGAGGTGTACAGGCACCAGGGGAATACA
GCCGGCTTCTTCTACATCGACTCAGATGGCAGCGGCCCACTGGGACCTCTCCAGGTGTAC
TGCAATATCACTGAGGACAAGATCTGGACATCAGTGCAGCACAACAATACAGAGCTGACC
CGAGTGCGGGGCGCTAACCCTGAGAAGCCCTATGCCATGGCCTTGGACTACGGGGGCAGC
ATGGAACAGCTGGAGGCCGTGATCGACGGCTCTGAGCACTGTGAGCAGGAGGTGGCCTAC
CACTGCAGGAGGTCCCGCCTGCTCAACACGCCGGATGGAACACCATTTACCTGGTGGATT
GGGCGGTCCAATGAAAGGCACCCTTACTGGGGAGGTTCCCCTCCTGGGGTCCAGCAGTGT
GAGTGTGGCCTAGACGAGAGCTGCCTGGACATTCAGCACTTTTGCAATTGCGACGCTGAC
AAGGATGAATGGACAAATGATACTGGCTTTCTTTCCTTCAAAGACCACTTGCCTGTCACT
CAGATAGTTATCACTGATACCGACAGATCAAACTCAGAAGCCGCTTGGAGAATTGGTCCC
TTGCGTTGCTATGGTGACCGACGCTTCTGGAACGCCGTCTCATTTTATACAGAAGCCTCT
TACCTCCACTTTCCTACCTTCCATGCGGAATTCAGTGCCGATATTTCCTTCTTTTTTAAA
ACCACAGCATTATCCGGAGTTTTCCTAGAAAATCTTGGCATTAAAGACTTCATTCGACTC
GAAATAAGCTCTCCTTCAGAGATCACCTTTGCCATCGATGTTGGGAATGGTCCTGTGGAG
CTTGTAGTCCAGTCTCCTTCTCTTCTGAATGACAACCAATGGCACTATGTCCGGGCTGAG
AGGAACCTCAAGGAGACCTCCCTGCAGGTGGACAACCTTCCAAGGAGCACCAGGGAGACG
TCGGAGGAGGGCCATTTTCGACTGCAGCTGAACAGCCAGTTGTTTGTAGGGGGAACGTCA
TCCAGACAGAAAGGCTTCCTAGGATGCATTCGCTCCTTACACTTGAATGGACAGAAAATG
GACCTGGAAGAGAGGGCAAAGGTCACATCTGGAGTCAGGCCAGGCTGCCCCGGCCACTGC
AGCAGCTACGGCAGCATCTGCCACAACGGGGGCAAGTGTGTGGAGAAGCACAATGGCTAC
CTGTGTGATTGCACCAATTCACCTTATGAAGGGCCCTTTTGCAAAAAAGAGGTTTCTGCT
GTTTTTGAGGCTGGCACGTCGGTTACTTACATGTTTCAAGAACCCTATCCTGTGACCAAG
AATATAAGCCTCTCATCCTCAGCTATTTACACAGATTCAGCTCCATCCAAGGAAAACATT
GCACTTAGCTTTGTGACAACCCAGGCACCCAGTCTTTTGCTCTTTATCAATTCTTCTTCT
CAGGACTTCGTGGTTGTTCTGCTCTGCAAGAATGGAAGCTTACAGGTTCGCTATCACCTA
AACAAGGAAGAAACCCATGTATTCACCATTGATGCAGATAACTTTGCTAACAGAAGGATG
CACCACTTGAAGATTAACCGAGAGGGAAGAGAGCTTACCATTCAGATGGACCAGCAACTT
CGACTCAGTTATAACTTCTCTCCGGAAGTAGAGTTCAGGGTTATAAGGTCACTCACCTTG
GGCAAAGTCACAGAGAATCTTGGTTTGGATTCTGAAGTTGCTAAAGCAAATGCCATGGGT
TTTGCTGGATGCATGTCTTCCGTCCAGTACAACCACATAGCACCACTGAAGGCTGCCCTG
CGCCATGCCACTGTCGCGCCTGTGACTGTCCATGGGACCTTGACGGAATCCAGCTGTGGC
TTCATGGTGGACTCAGATGTGAATGCAGTGACCACGGTGCATTCTTCATCAGATCCTTTT
GGGAAGACAGATGAGCGGGAACCACTCACAAATGCTGTTCGAAGTGATTCGGCAGTCATC
GGAGGGGTGATAGCAGTGGTGATATTCATCATCTTCTGTATCATCGGCATCATGACCCGG
TTCCTCTACCAGCACAAGCAGTCACATCGTACGAGCCAGATGAAGGAGAAGGAATATCCA
GAAAATTTGGACAGTTCCTTCAGAAATGAAATTGACTTGCAAAACACAGTGAGCGAGTGT
AAACGGGAATATTTCATC
Protein sequence predicted based on nucleotide sequence
MDSLPRLTSVLTLLFSGLWHLGLTATNYNCDDPLASLLSPMAFSSSSDLTGTHSPAQLNW RVGTGGWSPADSNAQQWLQMDLGNRVEITAVATQGRYGSSDWVTSYSLMFSDTGRNWKQY KQEDSIWTFAGNMNADSVVHHKLLHSVRARFVRFVPLEWNPSGKIGMRVEVYGCSYKSDV ADFDGRSSLLYRFNQKLMSTLKDVISLKFKSMQGDGVLFHGEGQRGDHITLELQKGRLAL HLNLGDSKARLSSSLPSATLGSLLDDQHWHSVLIERVGKQVNFTVDKHTQHFRTKGETDA LDIDYELSFGGIPVPGKPGTFLKKNFHGCIENLYYNGVNIIDLAKRRKHQIYTGNVTFSC SEPQIVPITFVNSSGSYLLLPGTPQIDGLSVSFQFRTWNKDGLLLSTELSEGSGTLLLSL EGGILRLVIQKMTERVAEILTGSNLNDGLWHSVSINARRNRITLTLDDEAAPPAPDSTWV QIYSGNSYYFGGCPDNLTDSQCLNPIKAFQGCMRLIFIDNQPKDLISVQQGSLGNFSDLH IDLCSIKDRCLPNYCEHGGSCSQSWTTFYCNCSDTSYTGATCHNSIYEQSCEVYRHQGNT AGFFYIDSDGSGPLGPLQVYCNITEDKIWTSVQHNNTELTRVRGANPEKPYAMALDYGGS MEQLEAVIDGSEHCEQEVAYHCRRSRLLNTPDGTPFTWWIGRSNERHPYWGGSPPGVQQC ECGLDESCLDIQHFCNCDADKDEWTNDTGFLSFKDHLPVTQIVITDTDRSNSEAAWRIGP LRCYGDRRFWNAVSFYTEASYLHFPTFHAEFSADISFFFKTTALSGVFLENLGIKDFIRL EISSPSEITFAIDVGNGPVELVVQSPSLLNDNQWHYVRAERNLKETSLQVDNLPRSTRET SEEGHFRLQLNSQLFVGGTSSRQKGFLGCIRSLHLNGQKMDLEERAKVTSGVRPGCPGHC SSYGSICHNGGKCVEKHNGYLCDCTNSPYEGPFCKKEVSAVFEAGTSVTYMFQEPYPVTK NISLSSSAIYTDSAPSKENIALSFVTTQAPSLLLFINSSSQDFVVVLLCKNGSLQVRYHL NKEETHVFTIDADNFANRRMHHLKINREGRELTIQMDQQLRLSYNFSPEVEFRVIRSLTL GKVTENLGLDSEVAKANAMGFAGCMSSVQYNHIAPLKAALRHATVAPVTVHGTLTESSCG FMVDSDVNAVTTVHSSSDPFGKTDEREPLTNAVRSDSAVIGGVIAVVIFIIFCIIGIMTR FLYQHKQSHRTSQMKEKEYPENLDSSFRNEIDLQNTVSECKREYFI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
365
End on reference sequence
4285
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
