Gene:
Gene Symbol:
CRYBG3
Gene Name:
beta-gamma crystallin domain containing 3
Original Clone ID:
FLH264838.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080219
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCAGATTTTCAACCTGGGGATACCACAGTAAGACTAGACAAAAGAATGTCTCTTACT
GCAATATATGACAAGAGGAGAGAGACAGATTATAGTGACAAAGGATATAATTTAGCTTTT
GTTTCTCAAGATGAACAAGAAAATTCTTCCTTTACTATATTATACGAAGAGCCCCTTCAA
GAGGAGGACAAGTATGCTTCCGCAGAAGCAAGACAAACACAGTCTGTCTTGTTTCATGAT
ACGTCCGCTGACAGCATGCCTGTTCTGGCATGTGAAAGGTCTGAGAGTAGAACTGACCTT
GTCCATCACTTTGAAAAAGGTACTAAATTAGGTGAGACATTTGATAGTGATAGTTCAGAA
ATGTTCTTATCAGTGGAGGCCAAAAGGTACAAAATTTATCCTTTAGCATTGTCTCCCATT
TATGAGGATGACAGCTCACAGGAGGACATTCTATCTAGTGAGGTTTCACCTGGTCACCAT
GGCCCCAGGAAATCAAGAGACAGTGAAAACCAGTCCTCTTCTGTTTTATCCCTTCTCCAG
TCAGTGTCAGAACGTTTAAAGATGAATTTTGATGAAGATGACAGAGAGGCAGCTGATGAG
GAAGAAGAGGAGGAGGAGGCAGCAGTATTGCATAAAGGAGATCTGAGAGCTGGAAGTGGG
GAGCGTGTTACCTTCCAGTTGCCAGATCCTTCCATCACATTTTACCCTGATGACCAGGAG
AGCGTTGGAATTTCTAAGAATTCATATGTGATGCCAAATGAACCTACTACCTCCAATCTG
CAAGTTGGTCTGTGGCCAGAAAAGACCTCGTTTCTCCAGAAATCTGACCTTACTTCTAAA
CTACATTCTTCTTTAAAGAGTGCTTATCATCAGTATCTGCAGACTTCCCAAAGTCATTCC
TCAGAAAAAGGAGCCAGATTTGGTGGAATTTTTCAGGAACCAGTGTCAAAATATTTCCGT
GTTCAAGACAGCCCAGGCAGATTGAGCCCATTTATAGAGAATGTTGACAAACAAACTCTG
AGATGTAACCCAAGACCTGGGAAGATGGTTATCTATGATCTCCATGAAAGTACATATAAA
CAAGAAGTCTACTGTAATATTCCTGATGCTACATCATGGTCTTTTCCAAATGGAGTTCTA
ATAAAAGTTGTAAGGGGCTGCTGGATTTTATATGAGAAACCACATTTCCGAGGTCAGAAA
TGTGTGCTAGAAGAAGGGGAAAAGGTGTTAAATCGTGACTGGATTCTTCAGAACAGAAGG
CATCCACAAAGAAACTTTATATTGGGTTCTCTCAAACGTGTCTTAAAGGACTGCAGCATT
CCAGAAATAGAGCTTTTCCCACAATCTGACCCAGCCTGTTGTCCTGTCTACATACAGAGA
GCAGTCCCCAATTTGGAAGAACTGAATATCTCCAAATCTGTGTCCTTCACTGTGAAGTCA
GGAGTTTGGCTTGCCTACCCAGATATTAATTTTAAGGGACAAGCTACAGTTCTGGAGGAA
GACCATGGGCTCTTTGAGATTTCTACAGCAGAAATGAAATCATTACATCCGCTTCAAATG
GGTGGACTGAAAGTGGAAATGCCCATGAACTTAAAGGTTATTATTTATGAAAAACCTCAC
TTCCATGGACAGGCTAAAGAGTTTAGTGAACATATAGATTCTGTTCCTAATTTTTTGAAA
AATAATGGAGATTTTCACAGAATTGGATCAATTCGTGTCATTGGTGGAGTGTGGGTTGCC
TATGAAAAAGAACATTTTAAAGGCCAGCAGTTTCTGCTTGAAGAAGGAGACTTTGAAGAC
AGTAATGCTTGTGGTGCATTAAGTAGCCCTATCTTGTCTTTCCGGTACTTACAAGCTAAT
TTTATAGAATCTTCTGTCACACTATTTGAATCTGACCTAGAAAGTGGGAAGTTTATTGAC
ATTACAAATCAGGAAATTTCTGATTTGGAAGAAATTGGCTTTGGCAGTAAAACAAGATCC
ATTCATGTTAAAAGTGGAGTATGGGTTGCCTACCAGCAAAAGTTCTTCTGTGGAGAACAA
TACATTTTAGAAAAAGGGAAATACAAATGCTTTTTTGACTGGGGAGGATCAAATAATATA
ATCATGTCGATACGGCCAATCCAACTGGAACCACTTGGGATAAATGAACCTCCGCATTTG
CTCAAAGCATTCAGCAAACCAGGGTTCCAAGGTGAATGTATAGATTTTACAGAAGAAACT
TCTGATTTGACTTCACTCATGCCATGTTCTTTTAAAGTTCTTCGAGGTTGCTGGCTCCTC
TATTACCAAGAAGACATGTTTGTTAATCACTGTGTGTTAGAAGAAGGCCTCTATGCTGAC
CTTACTTCCTGCGGTTGCCCAGCATCTAAAGTCAAATCTCTCAAGCCCATTGACTATGTT
TTTGAAGAACCCTCCATCAGCCTTTTTGCTCTGGAGCATTGTGAGGGAAGAGAGTTACAT
CTAGAAGAGGCTGTGAACTCTGTTCTGAACAAGGACCTACACTTCTACACCCAGTCTGTG
TGGGTAAAAAGTGGACTATGGATAGCTTATGAAGGATCCAATTTCTTGGGAAGACAAATC
CTACTCAGGCCTAATGAGATCCCAAACTGGACAGCATTCAGCAGATGGAAAACAATTGGT
TCCCTCCGTCCTATGAAGCAGCCTGCAGTGTACATCAGAATAAAGAACCGTGCCCAGGGT
GAATATCTGACAGTCACTGGAAGTCTAGCAGACACCAGGGCAACATCTGTGTGCATTTCT
CCCTATAGTGGAAAGAATACTCAGATCTGGTACTACTGCCGAGGACTCTTTAAATCCAAG
GCCAGTGATACATGTCTTGATGTGATTGGTGGCCGGGACACACCTGGAGCTAAAGTAGCT
CTATGGACTGAACATGGGCAATTCAGGCAGAAGTGGAGACTGAATAAAAATGGAACTATC
AGCTCTTATCTCAGTGATCAACTTGTCCTTGATGTTAAAGGAGGAAATTATTGTGACAAG
ACTCATGTAATTGTAAATCAGCCCCTGGAGGGAGAAGAAACACAGAAATGGGACATTGAA
ATATTG
Protein sequence predicted based on nucleotide sequence
MPDFQPGDTTVRLDKRMSLTAIYDKRRETDYSDKGYNLAFVSQDEQENSSFTILYEEPLQ EEDKYASAEARQTQSVLFHDTSADSMPVLACERSESRTDLVHHFEKGTKLGETFDSDSSE MFLSVEAKRYKIYPLALSPIYEDDSSQEDILSSEVSPGHHGPRKSRDSENQSSSVLSLLQ SVSERLKMNFDEDDREAADEEEEEEEAAVLHKGDLRAGSGERVTFQLPDPSITFYPDDQE SVGISKNSYVMPNEPTTSNLQVGLWPEKTSFLQKSDLTSKLHSSLKSAYHQYLQTSQSHS SEKGARFGGIFQEPVSKYFRVQDSPGRLSPFIENVDKQTLRCNPRPGKMVIYDLHESTYK QEVYCNIPDATSWSFPNGVLIKVVRGCWILYEKPHFRGQKCVLEEGEKVLNRDWILQNRR HPQRNFILGSLKRVLKDCSIPEIELFPQSDPACCPVYIQRAVPNLEELNISKSVSFTVKS GVWLAYPDINFKGQATVLEEDHGLFEISTAEMKSLHPLQMGGLKVEMPMNLKVIIYEKPH FHGQAKEFSEHIDSVPNFLKNNGDFHRIGSIRVIGGVWVAYEKEHFKGQQFLLEEGDFED SNACGALSSPILSFRYLQANFIESSVTLFESDLESGKFIDITNQEISDLEEIGFGSKTRS IHVKSGVWVAYQQKFFCGEQYILEKGKYKCFFDWGGSNNIIMSIRPIQLEPLGINEPPHL LKAFSKPGFQGECIDFTEETSDLTSLMPCSFKVLRGCWLLYYQEDMFVNHCVLEEGLYAD LTSCGCPASKVKSLKPIDYVFEEPSISLFALEHCEGRELHLEEAVNSVLNKDLHFYTQSV WVKSGLWIAYEGSNFLGRQILLRPNEIPNWTAFSRWKTIGSLRPMKQPAVYIRIKNRAQG EYLTVTGSLADTRATSVCISPYSGKNTQIWYYCRGLFKSKASDTCLDVIGGRDTPGAKVA LWTEHGQFRQKWRLNKNGTISSYLSDQLVLDVKGGNYCDKTHVIVNQPLEGEETQKWDIE IL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3133
Start on reference sequence
65
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
