Gene:
Gene Symbol:
SCRIB
Gene Name:
scribbled planar cell polarity protein
Original Clone ID:
FLH264939.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080318
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCTCAAGTGCATCCCGCTGTGGCGCTGCAACCGGCACGTGGAGTCGGTGGACAAGCGG
CACTGTTCGCTGCAGGCCGTGCCGGAGGAGATCTACCGCTACAGCCGCAGCCTGGAGGAG
CTGCTGCTCGACGCCAACCAGCTGCGCGAGCTGCCCAAGCCTTTTTTCCGGCTGCTGAAC
TTGCGCAAGCTGGGCCTGAGCGACAACGAGATCCAGCGGTTGCCTCCCGAGGTGGCCAAC
TTCATGCAGCTGGTGGAGCTGGACGTGTCCCGGAACGATATCCCTGAGATCCCGGAGAGC
ATCAAGTTCTGCAAGGCTCTGGAGATCGCGGACTTCAGCGGGAACCCCCTCTCCAGGCTC
CCTGATGGCTTCACTCAGCTGCGCAGCCTGGCTCACCTGGCCCTGAATGATGTGTCTCTG
CAGGCACTGCCCGGGGACGTGGGCAACCTCGCCAACCTGGTGACCCTGGAGCTCCGGGAG
AACCTGCTCAAGTCCCTGCCAGCGTCCCTGTCATTTCTGGTCAAGCTGGAACAGCTGGAT
CTGGGAGGCAACGATCTGGAAGTGCTGCCAGACACTCTGGGGGCTCTGCCCAATCTTCGG
GAGCTGTGGCTTGACCGGAACCAGCTGTCAGCACTGCCCCCGGAGCTCGGGAACCTGCGG
CGCCTGGTGTGCCTGGACGTGTCGGAAAACCGGCTGGAGGAGCTGCCTGCTGAGCTCGGC
GGGCTGGTGCTGCTCACTGACCTGCTGCTGTCCCAGAACCTGCTGCGGAGGCTGCCCGAC
GGCATCGGTCAGCTGAAGCAGCTATCCATCCTAAAGGTAGACCAGAATCGGCTGTGCGAG
GTGACTGAGGCCATCGGGGACTGTGAGAACCTCTCTGAGCTGATCCTCACGGAGAACCTG
CTGATGGCCCTGCCCCGCTCCCTGGGAAAGCTGACTAAGCTGACCAACCTCAACGTGGAC
CGGAACCACCTCGAGGCGCTGCCGCCCGAGATCGGGGGCTGTGTGGCACTCAGCGTCCTC
TCCTTGAGGGACAACCGCCTGGCCGTCCTGCCACCAGAGCTGGCCCACACGACAGAGCTG
CACGTGCTGGACGTGGCGGGGAACCGCCTGCAGAGTCTGCCGTTCGCGCTCACCCACCTC
AATCTCAAGGCCCTGTGGCTGGCAGAGAACCAGGCGCAGCCCATGCTCCGGTTCCAGACG
GAGGATGATGCCCGGACCGGCGAGAAGGTGCTCACCTGCTACTTGCTGCCCCAGCAGCCC
CCACTCAGCCTCGAGGATGCTGGGCAGCAGGGGAGCCTCTCGGAGACCTGGAGCGATGCC
CCGCCGAGCCGCGTCAGCGTCATCCAGTTCCTGGAGGCCCCCATAGGTGATGAGGACGCT
GAGGAAGCTGCAGCTGAGAAGCGGGGCCTACAGCGCCGGGCCACACCTCACCCCAGCGAG
CTCAAGGTGATGAAGAGGAGCATCGAGGGGCGGCGGAGCGAGGCCTGCCCTTGCCAGCCA
GACTCTGGGTCGCCCTTGCCTGCAGAGGAGGAGAAGCGGCTGAGTGCCGAGTCTGGCCTG
AGTGAAGACTCTCGCCCATCTGCCAGCACAGTCTCTGAGGCTGAGCCCGAGGGCCCGTCG
GCTGAGGCACAGGGTGGGAGCCAGCAGGAAGCCACGACTGCTGGCGGGGAGGAAGACGCC
GAAGAGGACTACCAGGAGCCCACGGTGCATTTCGCAGAGGACGCACTGCTGCCCGGGGAT
GACAGGGAGATCGAGGAGGGGCAGCCTGAGGCCCCCTGGACCCTGCCAGGCGGGAGGCAG
CGGCTCATCCGCAAGGACACACCTCACTACAAAAAGCACTTCAAGATCTCCAAGCTGCCC
CAGCCCGAGGCCGTTGTGGCTCTGCTGCAGGGCATGCAGCCTGATGGGGAGGGCCCTGTG
GCTCCCGGGGGCTGGCACAATGGCCCCCACGCACCCTGGGCTCCTCGGGCCCAGAAGGAG
GAGGAGGAGGAGGAAGAGGGTAGTCCTCAGGAGGAGGAGGAAGAGGAGGAGGAGGAAAAC
AGGGCTGAAGAGGAAGAGGCCAGCACTGAGGAGGAGGACAAGGAGGGGGCCGTGGTTTCT
GCGCCCTCTGTCAAGGGAGTGTCGTTTGACCAGGCCAATAACCTGCTGATAGAGCCTGCT
CGCATTGAGGAGGAAGAGCTGACCCTCACCATCCTGCGGCAGACTGGGGGCCTGGGCATC
AGCATTGCGGGCGGCAAGGGCTCCACACCCTATAAGGGGGACGACGAGGGCATATTCATC
TCTCGGGTGTCCGAGGAAGGCCCTGCGGCCCGGGCTGGAGTCCGTGTGGGTGACAAGCTC
CTGGAGGTGAATGGTGTGGCTCTGCAGGGCGCCGAGCACCACGAGGCCGTGGAGGCGCTC
CGGGGGGCCGGCACTGCCGTGCAGATGCGAGTGTGGCGGGAGCGCATGGTGGAGCCTGAG
AACGCGGTCACCATCACGCCGCTGCGGCCCGAGGATGATTACAGCCCCCGAGAGCGGCGG
GGAGGGGGGCTGCGCCTGCCCCTGCTCCCGCCTGAGAGCCCCGGGCCCCTCCGTCAGCGC
CACGTGGCCTGCCTGGCACGCAGCGAGAGGGGGCTGGGCTTCAGCATTGCTGGTGGGAAA
GGCTCCACACCCTACAGGGCTGGTGATGCGGGCATCTTCGTCTCCCGCATTGCCGAGGGC
GGTGCTGCTCACCGCGCGGGCACACTGCAGGTTGGCGACCGCGTCCTCTCTATTAATGGA
GTGGACGTGACTGAGGCCAGGCATGACCACGCCGTCTCCCTGCTGACCGCTGCCTCCCCC
ACCATCGCCCTGCTGTTGGAGCGGGAGGCTGGGGGCCCTCTTCCTCCCAGCCCTCTGCCA
CATTCCTCACCCCCCACCGCTGCTGTTGCCACCACCAGCATAACCACTGCCACCCCCGGG
GTGCCTGGGTTGCCGAGCCTGGCCCCCAGCCTGCTGGCTGCCGCGTTGGAAGGGCCATAC
CCAGTGGAGGAGATCCGTCTGCCAAGAGCTGGGGGCCCTCTGGGGCTTAGTATTGTCGGA
GGCTCCGACCATTCCAGCCACCCGTTTGGTGTCCAGGAGCCTGGTGTGTTCATCTCCAAG
GTGCTCCCGCGGGGCCTGGCCGCTCGCAGCGGCCTGCGGGTTGGGGACCGCATCCTGGCA
GTGAACGGGCAAGACGTGCGGGATGCCACGCACCAAGAAGCAGTCAGTGCCCTGCTCCGG
CCCTGCCTGGAGCTGTCGCTGCTGGTGCGGAGGGACCCGGCACCCCCGGGCCTACGGGAA
CTGTGCATCCAGAAGGCACCTGGGGAGAGGCTGGGCATCAGCATCCGCGGGGGTGCCAGG
GGCCACGCTGGCAACCCCCGCGACCCCACAGACGAGGGCATCTTCATCTCCAAGGTGAGC
CCCACGGGGGCAGCCGGGCGCGACGGTCGGCTGCGTGTGGGTTTGCGGCTGTTGGAGGTG
AACCAGCAGAGCCTGCTGGGCCTGACGCACGGCGAGGCGGTGCAGCTGCTCCGCAGTGTG
GGCGACACCCTCACCGTGCTGGTCTGTGACGGCTTCGAGGCCAGCACCGACGCAGCCCTG
GAGGTGTCCCCAGGTGTCATTGCCAACCCCTTTGCGGCAGGCATCGGCCACCGGAACAGC
CTGGAGAGCATCTCTTCCATCGACCGGGAGCTGAGCCCTGAGGGCCCAGGCAAGGAGAAG
GAGCTGCCTGGACAGACCCTGCACTGGGGGCCCGAGGCCACAGAAGCCGCAGGTCGGGGT
CTGCAGCCCCTGAAGCTGGACTACCGCGCCCTGGCCGCCGTGCCCAGCGCTGGCAGCGTG
CAGAGGGTACCGTCTGGAGCAGCTGGAGGGAAGATGGCTGAATCTCCCTGCTCCCCTAGT
GGCCAGCAGCCGCCCTCCCCGCCTTCTCCGGATGAGCTGCCCGCCAATGTGAAGCAGGCC
TACAGGGCCTTCGCGGCCGTGCCCACTTCTCACCCGCCTGAGGATGCCCCTGCCCAGCCC
CCCACGCCTGGGCCTGCAGCCTCCCCGGAGCAGCTGTCCTTCCGGGAGCGGCAGAAGTAC
TTTGAGCTGGAGGTGCGCGTGCCCCAGGCCGAGGGCCCCCCTAAGCGCGTGTCCCTGGTG
GGTGCTGACGACCTGCGGAAGATGCAGGAGGAGGAAGCCAGAAAACTACAGCAGAAGAGA
GCGCAGATGCTGCGGGAGGCGGCAGAGGCTGGGGCCGAAGCGAGGCTCGCCCTGGACGGG
GAGACGCTGGGCGAGGAGGAACAGGAGGATGAGCAGCCACCCTGGGCCAGCCCGAGCCCC
ACCTCAAGGCAGAGCCCGGCGTCCCCCCCGCCCCTGGGAGGTGGCGCCCCGGTGCGGACG
GCCAAAGCTGAACGGCGCCACCAGGAGCGGCTGCGCGTGCAGAGTCCGGAGCCACCGGCA
CCCGAGCGTGCCCTGTCCCCTGCCGAGCTCCGGGCCCTGGAGGCCGAGAAGCGTGCGCTG
TGGAGGGCAGCCAGGATGAAGTCATTGGAACAGGACGCTCTCCGAGCACAGATGGTCCTC
AGCAGGTCCCAGGAAGGCCGGGGCACGCGGGGGCCCCTGGAGCGACTGGCCGAGGCCCCT
TCCCCTGCGCCCACCCCGTCGCCCACCCCTGTGGAAGACCTCGGCCCCCAGACCAGCACC
TCCCCGGGACGCCTGCCCTTGTCTGGAAAGAAGTTTGACTACAGGGCCTTTGCGGCCCTG
CCTTCTTCCAGACCTGTCTATGACATCCAGTCACCGGACTTTGCTGAGGAGTTGAGGTCC
CTGGAACCATCTCCCAGCCCTGGCCCGCAGGAGGAGGATGGAGAAGTGGCTCTGGTGCTT
CTGGGCAGGCCCTCACCCGGCGCTGTGGGCCCTGAAGATGTGGCACTGTGCAGCAGCCGC
CGCCCCGTAAGGCCTGGGCGCCGTGGCCTGGGCCCTGTGCCCTCC
Protein sequence predicted based on nucleotide sequence
MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLN LRKLGLSDNEIQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRL PDGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLD LGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPAELG GLVLLTDLLLSQNLLRRLPDGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENL LMALPRSLGKLTKLTNLNVDRNHLEALPPEIGGCVALSVLSLRDNRLAVLPPELAHTTEL HVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDARTGEKVLTCYLLPQQP PLSLEDAGQQGSLSETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSE LKVMKRSIEGRRSEACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSEAEPEGPS AEAQGGSQQEATTAGGEEDAEEDYQEPTVHFAEDALLPGDDREIEEGQPEAPWTLPGGRQ RLIRKDTPHYKKHFKISKLPQPEAVVALLQGMQPDGEGPVAPGGWHNGPHAPWAPRAQKE EEEEEEGSPQEEEEEEEEENRAEEEEASTEEEDKEGAVVSAPSVKGVSFDQANNLLIEPA RIEEEELTLTILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKL LEVNGVALQGAEHHEAVEALRGAGTAVQMRVWRERMVEPENAVTITPLRPEDDYSPRERR GGGLRLPLLPPESPGPLRQRHVACLARSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAEG GAAHRAGTLQVGDRVLSINGVDVTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLP HSSPPTAAVATTSITTATPGVPGLPSLAPSLLAAALEGPYPVEEIRLPRAGGPLGLSIVG GSDHSSHPFGVQEPGVFISKVLPRGLAARSGLRVGDRILAVNGQDVRDATHQEAVSALLR PCLELSLLVRRDPAPPGLRELCIQKAPGERLGISIRGGARGHAGNPRDPTDEGIFISKVS PTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCDGFEASTDAAL EVSPGVIANPFAAGIGHRNSLESISSIDRELSPEGPGKEKELPGQTLHWGPEATEAAGRG LQPLKLDYRALAAVPSAGSVQRVPSGAAGGKMAESPCSPSGQQPPSPPSPDELPANVKQA YRAFAAVPTSHPPEDAPAQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLV GADDLRKMQEEEARKLQQKRAQMLREAAEAGAEARLALDGETLGEEEQEDEQPPWASPSP TSRQSPASPPPLGGGAPVRTAKAERRHQERLRVQSPEPPAPERALSPAELRALEAEKRAL WRAARMKSLEQDALRAQMVLSRSQEGRGTRGPLERLAEAPSPAPTPSPTPVEDLGPQTST SPGRLPLSGKKFDYRAFAALPSSRPVYDIQSPDFAEELRSLEPSPSPGPQEEDGEVALVL LGRPSPGAVGPEDVALCSSRRPVRPGRRGLGPVPS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4975
Start on reference sequence
8
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
