Gene:
Gene Symbol:
TBC1D4
Gene Name:
TBC1 domain family, member 4
Original Clone ID:
FLH264949.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080328
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCCCCTGGAGCCCGAGCCG
GGCGTCTCAGCTCAGCCCGGCCCCGGGAAGCCAAGCGATAAGCGGTTCCGGCTGTGGTAC
GTTGGGGGGTCGTGCCTGGACCACAGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCC
GAGATCCGCAGGCGCAGCCAGAAGCCCGAGGCGGGCGGCTGCGGGGCGCCGGCGGCCCGA
GAGGTGATCCTGGTGCTCAGCGCGCCCTTCCTGCGTTGCGTCCCCGCGCCGGGCGCTGGG
GCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGCGGTATTCATCTTCGAG
CACAAGGCGCAGCATATCTCGCGCTTCATCCACAACAGCCACGACCTCACCTACTTTGCC
TACCTGATCAAGGCGCAGCCCGACGACCCCGAGTCGCAGATGGCCTGCCACGTTTTCCGC
GCCACAGACCCCAGCCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCG
GCCATGAAAGAGGATGCCAAACCCAGCAAAGATAATGAGGACGCCTTTTACAACTCTCAG
AAGTTCGAAGTCCTGTACTGTGGAAAGGTGACCGTGACCCACAAGAAGGCCCCCTCAAGC
CTCATCGATGACTGCATGGAGAAGTTCAGCCTGCACGAACAGCAGCGCCTGAAGATCCAA
GGGGAGCAGCGCGGTCCGGACCCAGGAGAGGACCTGGCTGACTTGGAGGTGGTGGTGCCC
GGGTCCCCCGGAGACTGCCTGCCGGAGGAGGCTGACGGCACCGACACCCACCTTGGCTTA
CCTGCCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAGCGGATT
TTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCGGTGCAGCAGTGTCACC
GGCGTGCAACGGAGAGTTCACGAGGGCAGCCAGAAATCCCAGCCGCGACGGAGACACGCG
AGCGCACCCAGTCACGTCCAGCCCTCGGACTCGGAGAAGAACAGGACCATGCTCTTCCAG
GTTGGGCGATTTGAGATTAACCTTATCAGTCCAGACACTAAATCAGTTGTGCTAGAAAAG
AATTTTAAAGATATCTCCTCTTGTTCTCAGGGTATAAAGCATGTGGATCACTTTGGCTTT
ATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATTTGTTATGTATTCCAGTGT
GCCAGCGAATCTCTGGTTGATGAGGTAATGCTGACTCTGAAACAGGCCTTCAGTACGGCG
GCTGCCCTGCAGAGTGCCAAGACGCAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCT
TTGCATAAGCTCTGTGAAAGGATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATA
CAGAGGCATCTCTCATCACTGACAGATAATGAGCAAGCTGACATCTTTGAAAGAGTTCAG
AAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGAACTTGTGATTTTACACCTGAGGCAG
CTGTGTGAAGCCAAGCAGAAGACACACGTGCACATCGGGGAAGGCCCTTCTACTATTTCA
AATAGTACAATCCCAGAAAATGCAACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAA
AATAAAGCTAAGAGATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAGCTAAC
AGAATGAGAGGTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTTGCTTCA
GAGAAGGACTACTCACCAGGGGATTCTCCACCAGGGACACCGCCAGCGTCCCCACCGTCC
TCAGCTTGGCAAACGTTTCCCGAAGAGGATTCCGACTCCCCGCAGTTTCGAAGACGGGCA
CACACGTTCAGCCACCCACCTTCAAGCACAAAGAGAAAGCTGAATTTGCAGGATGGGAGG
GCTCAGGGTGTGCGTTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGCAATCTT
TCGTCAGTTCGACGCATGTACAAGGAGAGTAATTCTTCCTCCAGTCTTCCAAGTCTTCAC
ACTTCCTTCTCTGCCCCTTCCTTCACTGCCCCCTCTTTCCTGAAAAGCTTTTACCAGAAT
TCAGGTAGACTGTCCCCACAGTATGAAAATGAAATCAGACAAGACACTGCTTCAGAATCA
AGTGATGGAGAAGGGAGAAAAAGGACCTCATCTACCTGCAGCAATGAGTCCCTAAGTGTG
GGAGGAACCTCTGTCACTCCTCGCCGGATCTCCTGGCGGCAGCGCATTTTCCTCAGGGTT
GCTTCTCCCATGAACAAATCTCCCTCAGCAATGCAACAGCAAGATGGATTGGACAGGAAC
GAGCTGCTGCCACTGTCCCCCCTCTCTCCAACCATGGAGGAGGAACCGCTGGTTGTATTC
CTGTCTGGGGAGGATGACCCAGAAAAGATTGAAGAAAGAAAGAAATCAAAAGAACTGAGG
AGCTTGTGGAGAAAAGCTATACACCAACAAATCTTGTTACTTCGAATGGAAAAAGAAAAC
CAGAAACTTGAAGCAAGCAGAGATGAACTCCAGTCCAGAAAAGTTAAATTAGACTATGAA
GAAGTTGGTGCATGTCAGAAAGAGGTCTTAATAACTTGGGATAAGAAGTTGTTAAACTGC
AGAGCTAAAATCAGATGTGATATGGAAGATATTCATACTCTTCTTAAAGAAGGAGTTCCC
AAAAGTCGACGAGGAGAAATTTGGCAGTTTCTGGCTTTACAGTACCGACTCAGACACAGA
TTGCCTAATAAACAACAGCCTCCTGACATATCCTATAAGGAACTTTTGAAGCAGCTCACT
GCTCAGCAGCATGCGATTCTCGTGGATTTAGGAAGGACGTTTCCTACTCACCCTTACTTT
TCAGTACAGCTTGGGCCAGGACAGCTGTCACTGTTTAACCTCCTGAAAGCCTATTCTTTG
CTGGACAAAGAAGTGGGATACTGTCAGGGGATCAGCTTTGTGGCTGGAGTCCTGCTTCTG
CACATGAGTGAAGAGCAAGCCTTTGAAATGCTGAAATTCCTCATGTATGACCTCGGCTTC
CGCAAGCAGTACAGACCTGACATGATGTCGCTGCAGATTCAAATGTACCAGCTGTCCAGG
CTCCTTCATGACTATCACAGAGATCTCTACAATCACCTTGAAGAAAATGAAATCAGCCCC
AGTCTTTATGCTGCCCCCTGGTTCCTCACATTGTTTGCCTCTCAGTTTTCATTAGGATTT
GTAGCCAGAGTTTTTGATATTATTTTTCTTCAGGGAACTGAAGTTATATTCAAGGTTGCA
CTCAGCCTACTGAGCAGCCAAGAGACACTTATAATGGAATGTGAGAGCTTTGAAAATATT
GTTGAGTTTCTTAAAAACACGCTACCTGATATGAATACCTCTGAAATGGAAAAAATTATT
ACCCAGGTTTTTGAGATGGATATTTCTAAGCAGTTGCATGCCTATGAGGTGGAATATCAT
GTGCTACAGGATGAGCTTCAGGAATCTTCATATTCCTGTGAGGATAGTGAAACTTTGGAG
AAGCTGGAGAGGGCCAATAGCCAACTGAAAAGACAAAACATGGACCTCCTAGAAAAATTA
CAGGTAGCTCATACTAAAATCCAGGCCTTGGAATCAAACCTGGAAAATCTTTTGACGAGA
GAGACCAAAATGAAGTCTTTAATCCGGACCCTGGAACAAGAAAAAATGGCTTATCAAAAG
ACAGTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGCTCTAGTCAATTGTGACCTGTTG
CTGAGAGACCTAAACTGCAACCCTAACAACAAAGCCAAGATAGGAAATAAGCCA
Protein sequence predicted based on nucleotide sequence
MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMA EIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFE HKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKA AMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQ GEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERI LEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQ VGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYICYVFQC ASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVI QRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPSTIS NSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLAS EKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGR AQGVRSPLLRQSSSEQCSNLSSVRRMYKESNSSSSLPSLHTSFSAPSFTAPSFLKSFYQN SGRLSPQYENEIRQDTASESSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRV ASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVVFLSGEDDPEKIEERKKSKELR SLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNC RAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLT AQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLL HMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISP SLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENI VEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLE KLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQK TVEQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
348
End on reference sequence
4244
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
