Gene:
Gene Symbol:
HELLS
Gene Name:
helicase, lymphoid-specific
Original Clone ID:
FLH264950.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080329
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCAGCGGAACGGCCCGCGGGCAGCGGCGGCTCGGAGGCTCCAGCAATGGTTGAACAA
CTGGACACTGCTGTGATTACCCCGGCCATGCTAGAAGAGGAAGAACAGCTTGAAGCTGCT
GGACTAGAGAGAGAGCGGAAGATGCTGGAAAAGGCTCGCATGTCTTGGGATAGAGAGTCG
ACAGAAATTCGGTACCGTAGACTTCAACATTTGCTTGAAAAAAGCAATATATACTCCAAA
TTTTTATTGACGAAAATGGAACAGCAACAATTAGAGGAACAGAAGAAGAAAGAAAAATTG
GAGAGAAAAAAGGAGTCTTTAAAAGTTAAAAAGGGTAAAAATTCAATTGATGCAAGTGAA
GAGAAGCCAGTTATGAGGAAAAAAAGAGGAAGAGAAGATGAATCATACAATATTTCAGAG
GTCATGTCAAAAGAGGAAATTTTGTCTGTGGCTAAAAAAAATAAAAAGGAGAATGAGGAT
GAAAACTCCTCCTCTACTAATCTCTGTGTGGAAGATCTTCAGAAAAATAAAGATTCGAAT
AGTATAATTAAAGATAGATTGTCTGAAACGGTTAGGCAGAATACTAAATTCTTTTTTGAC
CCAGTCCGGAAGTGTAATGGTCAGCCAGTACCTTTTCAACAACCAAAGCACTTCACTGGA
GGAGTGATGCGATGGTACCAAGTAGAAGGCATGGAATGGCTTAGGATGCTTTGGGAAAAT
GGAATTAATGGCATTTTAGCAGATGAAATGGGATTGGGTAAGACAGTTCAGTGCATTGCT
ACTATTGCATTGATGATTCAGAGAGGAGTACCAGGACCTTTTCTTGTCTGTGGCCCTTTG
TCTACACTTCCTAACTGGATGGCTGAATTCAAAAGATTTACACCAGATATCCCTACAATG
TTATATCATGGAACCCAGGAGGAACGTCAAAAATTGGTAAGAAATATTTACAAACGGAAA
GGGACTTTGCAGATTCATCCTGTGGTAATCACGTCATTTGAAATAGCCATGAGAGACCGA
AATGCGTTACAGCATTGCTATTGGAAATACTTAATAGTAGATGAAGGACACAGGATTAAG
AATATGAAGTGCCGTCTAATCAGGGAGTTAAAACGATTCAATGCTGATAACAAACTTCTT
TTGACTGGTACTCCCTTGCAAAACAATTTATCAGAACTTTGGTCATTGCTAAACTTTTTG
TTGCCAGATGTATTTGATGACTTGAAAAGCTTTGAGTCTTGGTTTGACATCACTAGTCTT
TCTGAAACTGCTGAAGATATTATTGCTAAAGAAAGAGAACAGAATGTATTGCATATGCTG
CACCAGATTTTAACACCTTTCTTATTGAGAAGACTGAAGTCTGATGTTGCTCTTGAAGTT
CCTCCTAAACGAGAAGTAGTCGTTTATGCTCCACTTTCAAAGAAGCAGGAGATCTTTTAT
ACAGCCATTGTGAACCGTACAATTGCAAACATGTTTGGATCCAGTGAGAAAGAAACAATT
GAGTTAAGTCCTACTGGTCGACCAAAACGACGAACTAGAAAATCAATAAATTACAGCAAA
ATAGATGATTTCCCTAATGAATTGGAAAAACTGATCAGTCAAATACAGCCAGAGGTGGAC
CGAGAAAGAGCTGTTGTGGAAGTGAATATCCCTGTAGAATCTGAAGTTAATCTGAAGCTG
CAGAATATAATGATGCTACTTCGTAAATGTTGTAATCATCCATATTTGATTGAATATCCT
ATAGACCCTGTTACACAAGAATTTAAGATCGATGAAGAATTGGTAACAAATTCTGGGAAG
TTCTTGATTTTGGATCGAATGCTGCCAGAACTAAAAAAAAGAGGTCACAAGGTGCTGCTT
TTTTCACAAATGACAAGCATGTTGGACATTTTGATGGATTACTGCCATCTCAGAGATTTC
AACTTCAGCAGGCTTGATGGGTCCATGTCTTACTCAGAGAGAGAAAAAAACATGCACAGC
TTCAACACGGATCCAGAGGTGTTTATCTTCTTAGTGAGTACACGAGCTGGTGGCCTGGGC
ATTAATCTGACTGCAGCAGATACAGTTATCATTTATGATAGTGATTGGAACCCCCAGTCG
GATCTTCAGGCCCAGGATAGATGTCATAGAATTGGTCAGACAAAGCCAGTTGTTGTTTAT
CGCCTTGTTACAGCAAATACTATCGATCAGAAAATTGTGGAAAGAGCAGCTGCTAAAAGG
AAACTGGAAAAGTTGATCATCCATAAAAATCATTTCAAAGGTGGTCAGTCTGGATTAAAT
CTGTCTAAGAATTTCTTAGATCCTAAGGAATTAATGGAATTATTAAAATCTAGAGATTAT
GAAAGGGAAATAAAAGGATCAAGAGAGAAGGTCATTAGTGATAAAGATCTAGAGTTGTTG
TTAGATCGAAGTGATCTTATTGATCAAATGAATGCTTCAGGACCAATTAAAGAGAAGATG
GGGATATTCAAGATATTAGAAAATTCTGAAGATTCCAGTCCTGAATGTTTGTTT
Protein sequence predicted based on nucleotide sequence
MPAERPAGSGGSEAPAMVEQLDTAVITPAMLEEEEQLEAAGLERERKMLEKARMSWDRES TEIRYRRLQHLLEKSNIYSKFLLTKMEQQQLEEQKKKEKLERKKESLKVKKGKNSIDASE EKPVMRKKRGREDESYNISEVMSKEEILSVAKKNKKENEDENSSSTNLCVEDLQKNKDSN SIIKDRLSETVRQNTKFFFDPVRKCNGQPVPFQQPKHFTGGVMRWYQVEGMEWLRMLWEN GINGILADEMGLGKTVQCIATIALMIQRGVPGPFLVCGPLSTLPNWMAEFKRFTPDIPTM LYHGTQEERQKLVRNIYKRKGTLQIHPVVITSFEIAMRDRNALQHCYWKYLIVDEGHRIK NMKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSL SETAEDIIAKEREQNVLHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLSKKQEIFY TAIVNRTIANMFGSSEKETIELSPTGRPKRRTRKSINYSKIDDFPNELEKLISQIQPEVD RERAVVEVNIPVESEVNLKLQNIMMLLRKCCNHPYLIEYPIDPVTQEFKIDEELVTNSGK FLILDRMLPELKKRGHKVLLFSQMTSMLDILMDYCHLRDFNFSRLDGSMSYSEREKNMHS FNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVY RLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDY EREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
106
End on reference sequence
2622
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
