Gene:
Gene Symbol:
ITGA8
Gene Name:
integrin, alpha 8
Original Clone ID:
FLH264990.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080369
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCGCCCGGGGCCAGCCGCGGTCCCCGGGGAAGCCAGGCGCCGCTGATCGCGCCCCTC
TGCTGCGCCGCGGCCGCGCTGGGGATGTTGCTGTGGTCCCCCGCCTGTCAGGCGTTCAAC
CTGGACGTGGAAAAGCTCACAGTGTACAGCGGCCCCAAGGGCAGCTACTTCGGCTACGCC
GTGGACTTCCACATACCCGACGCCCGCACAGCGAGTGTCTTGGTGGGGGCGCCCAAAGCC
AACACCAGCCAGCCCGATATCGTGGAAGGGGGAGCCGTCTATTACTGTCCTTGGCCCGCG
GAGGGGTCTGCGCAGTGCAGGCAGATACCGTTTGACACCACCAACAACAGAAAGATCAGA
GTTAATGGAACCAAAGAACCTATCGAGTTCAAATCCAATCAGTGGTTTGGAGCAACAGTG
AAAGCTCACAAAGGAAAAGTTGTGGCCTGTGCTCCTTTATATCACTGGAGAACTCTTAAA
CCGACACCAGAAAAGGACCCAGTTGGCACCTGCTATGTAGCAATTCAGAACTTCAGCGCC
TATGCCGAGTTCTCTCCTTGCCGGAACAGCAATGCTGATCCGGAAGGCCAGGGTTACTGC
CAAGCAGGATTTAGTCTGGATTTTTATAAGAATGGAGACCTTATTGTGGGAGGACCTGGG
AGTTTCTACTGGCAAGGACAAGTGATCACTGCCAGTGTTGCAGATATCATTGCAAATTAC
TCATTCAAGGATATCCTCAGGAAACTGGCAGGAGAAAAGCAGACGGAAGTGGCTCCAGCT
TCCTATGATGACAGTTACCTTGGATACTCAGTTGCTGCTGGGGAGTTTACTGGGGATTCT
CAGCAAGAATTGGTTGCTGGAATTCCAAGAGGAGCACAGAATTTTGGATATGTTTCCATC
ATTAACTCTACGGATATGACGTTTATTCAGAATTTCACGGGAGAACAGATGGCATCTTAT
TTTGGATATACCGTTGTCGTATCAGATGTTAACAGTGATGGACTGGATGATGTCCTGGTT
GGGGCACCTCTCTTTATGGAACGTGAATTTGAGAGCAACCCCAGAGAAGTAGGGCAAATC
TACCTGTATTTGCAAGTGAGCTCTCTCCTCTTCAGAGACCCCCAGATCCTCACTGGCACC
GAGACGTTTGGGAGATTCGGTAGTGCTATGGCACACTTAGGAGACCTGAACCAAGATGGA
TACAATGACATTGCCATCGGAGTGCCTTTTGCAGGCAAGGATCAAAGAGGCAAAGTGCTC
ATTTATAATGGGAACAAAGATGGCTTAAACACCAAGCCTTCCCAAGTTCTGCAAGGAGTG
TGGGCCTCACATGCTGTCCCTTCCGGATTTGGCTTTACTTTAAGAGGAGATTCAGACATA
GACAAGAATGATTACCCAGATTTGATTGTGGGTGCATTTGGAACAGGAAAAGTCGCTGTT
TACAGAGCAAGACCGGTTGTGACTGTAGATGCCCAGCTTCTGCTGCACCCAATGATTATC
AATCTTGAAAATAAAACTTGCCAGGTTCCAGACTCTATGACATCTGCTGCCTGCTTTTCT
TTAAGAGTATGTGCATCTGTCACAGGCCAGAGCATTGCAAACACAATAGTCTTGATGGCA
GAGGTGCAATTAGATTCCCTGAAACAGAAAGGAGCTATTAAACGGACGCTCTTCCTTGAT
AACCATCAGGCTCATCGCGTCTTCCCTCTTGTGATAAAAAGGCAGAAATCCCACCAGTGC
CAGGATTTCATCGTTTACCTTCGAGATGAAACTGAATTCCGAGATAAATTATCTCCAATC
AACATTAGTTTGAATTACAGTTTGGACGAATCCACCTTTAAAGAAGGCCTGGAAGTGAAA
CCAATATTGAACTACTACAGAGAAAACATTGTTAGTGAACAGGCTCACATTCTGGTGGAC
TGTGGAGAAGACAATCTGTGTGTTCCTGACTTGAAGCTGTCGGCTAGACCAGATAAGCAT
CAGGTAATCATTGGAGATGAAAATCACCTTATGCTCATAATAAATGCAAGAAATGAAGGG
GAAGGAGCATATGAAGCTGAACTCTTTGTAATGATACCAGAAGAGGCAGATTATGTTGGA
ATCGAACGCAACAACAAGGGATTTCGACCACTGAGCTGTGAGTACAAGATGGAAAATGTA
ACCAGGATGGTGGTGTGTGACCTTGGGAACCCTATGGTGTCTGGAACAAATTATTCCCTG
GGCCTCCGATTTGCAGTTCCACGTCTTGAGAAAACAAACATGAGCATTAACTTCGATCTC
CAAATCAGAAGTTCCAACAAGGACAATCCAGACAGCAATTTTGTGAGCCTGCAAATCAAC
ATCACTGCTGTAGCGCAGGTGGAAATAAGAGGAGTGTCACACCCTCCGCAGATTGTTCTG
CCCATTCATAACTGGGAACCAGAAGAGGAGCCCCACAAAGAGGAGGAGGTTGGACCATTG
GTGGAACATATTTATGAGCTGCACAATATTGGACCAAGTACCATCAGTGACACCATCCTG
GAGGTGGGCTGGCCTTTCTCTGCCCGGGATGAATTTCTTCTCTATATTTTCCATATTCAA
ACTCTGGGACCTCTGCAGTGCCAACCAAATCCTAATATCAATCCACAGGATATAAAGCCT
GCTGCCTCCCCAGAGGACACCCCTGAGCTCAGCGCCTTTTTGCGAAACTCTACTATTCCT
CATCTTGTCAGGAAGAGGGATGTACATGTGGTCGAATTCCACAGACAGAGCCCTGCAAAA
ATACTGAATTGTACAAATATCGAGTGTTTACAAATCTCCTGTGCAGTGGGACGACTCGAA
GGAGGAGAAAGCGCAGTCCTGAAAGTCAGGTCACGATTATGGGCCCACACCTTCCTCCAG
AGAAAAAATGATCCCTATGCTCTTGCATCCCTGGTGTCCTTTGAAGTTAAGAAGATGCCT
TATACAGATCAGCCAGCAAAACTCCCAGAAGGAAGCATAGCAATTAAGACATCAGTTATT
TGGGCAACTCCGAATGTTTCCTTCTCAATCCCATTATGGGTAATAATACTAGCAATACTT
CTTGGATTGTTGGTTCTCGCCATTTTAACCTTAGCTTTATGGAAGTGTGGATTCTTTGAC
AGAGCCAGACCTCCTCAGGAGGACATGACCGACAGGGAACAGCTGACAAATGACAAGACC
CCTGAGGCA
Protein sequence predicted based on nucleotide sequence
MSPGASRGPRGSQAPLIAPLCCAAAALGMLLWSPACQAFNLDVEKLTVYSGPKGSYFGYA VDFHIPDARTASVLVGAPKANTSQPDIVEGGAVYYCPWPAEGSAQCRQIPFDTTNNRKIR VNGTKEPIEFKSNQWFGATVKAHKGKVVACAPLYHWRTLKPTPEKDPVGTCYVAIQNFSA YAEFSPCRNSNADPEGQGYCQAGFSLDFYKNGDLIVGGPGSFYWQGQVITASVADIIANY SFKDILRKLAGEKQTEVAPASYDDSYLGYSVAAGEFTGDSQQELVAGIPRGAQNFGYVSI INSTDMTFIQNFTGEQMASYFGYTVVVSDVNSDGLDDVLVGAPLFMEREFESNPREVGQI YLYLQVSSLLFRDPQILTGTETFGRFGSAMAHLGDLNQDGYNDIAIGVPFAGKDQRGKVL IYNGNKDGLNTKPSQVLQGVWASHAVPSGFGFTLRGDSDIDKNDYPDLIVGAFGTGKVAV YRARPVVTVDAQLLLHPMIINLENKTCQVPDSMTSAACFSLRVCASVTGQSIANTIVLMA EVQLDSLKQKGAIKRTLFLDNHQAHRVFPLVIKRQKSHQCQDFIVYLRDETEFRDKLSPI NISLNYSLDESTFKEGLEVKPILNYYRENIVSEQAHILVDCGEDNLCVPDLKLSARPDKH QVIIGDENHLMLIINARNEGEGAYEAELFVMIPEEADYVGIERNNKGFRPLSCEYKMENV TRMVVCDLGNPMVSGTNYSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNFVSLQIN ITAVAQVEIRGVSHPPQIVLPIHNWEPEEEPHKEEEVGPLVEHIYELHNIGPSTISDTIL EVGWPFSARDEFLLYIFHIQTLGPLQCQPNPNINPQDIKPAASPEDTPELSAFLRNSTIP HLVRKRDVHVVEFHRQSPAKILNCTNIECLQISCAVGRLEGGESAVLKVRSRLWAHTFLQ RKNDPYALASLVSFEVKKMPYTDQPAKLPEGSIAIKTSVIWATPNVSFSIPLWVIILAIL LGLLVLAILTLALWKCGFFDRARPPQEDMTDREQLTNDKTPEA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3192
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
