Gene:
Gene Symbol:
NLRP5
Gene Name:
NLR family, pyrin domain containing 5
Original Clone ID:
FLH265077.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080456
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAAGGTTGCAGGAGGACTTGAACTTGGAGCTGCTGCTCTGCTCTCAGCATCACCACGT
GCTCTTGTCACTCTTTCCACAGGTCCTACTTGCTCTATATTACCAAAGAATCCACTTTTC
CCCCAAAACCTGAGCTCTCAGCCTTGTATCAAGATGGAAGGAGACAAATCGCTCACCTTT
TCCAGCTACGGGCTGCAATGGTGTCTCTATGAGCTAGACAAGGAAGAATTTCAGACATTC
AAGGAATTACTAAAGAAGAAATCTTCAGAATCGACCACATGCTCTATTCCACAGTTTGAA
ATCGAGAATGCCAACGTGGAATGTCTGGCACTCCTCTTGCATGAGTATTATGGAGCATCG
CTGGCCTGGGCTACGTCCATTAGCATCTTTGAAAACATGAACCTGCGAACCCTCTCGGAG
AAGGCACGGGATGACATGAAAAGACATTCACCAGAAGATCCTGAAGCAACGATGACTGAC
CAAGGACCAAGCAAGGAAAAAGTGCCAGGAATTTCACAAGCTGTGCAACAAGATAGTGCC
ACAGCTGCAGAGACAAAAGAACAAGAAATTTCACAAGCTATGGAACAAGAAGGTGCCACA
GCAGCAGAGACAGAAGAACAAGGACATGGAGGTGACACATGGGACTACAAGAGTCACGTG
ATGACCAAATTCGCTGAGGAGGAGGATGTACGTCGTAGTTTTGAAAACACTGCTGCTGAC
TGGCCGGAAATGCAAACGTTGGCTGGTGCTTTTGATTCAGACCGGTGGGGCTTCCGGCCT
CGCACGGTGGTTCTGCACGGAAAGTCAGGAATTGGGAAATCGGCTCTAGCCAGAAGGATC
GTGCTGTGCTGGGCGCAAGGTGGACTCTACCAGGGAATGTTCTCCTACGTCTTCTTCCTC
CCCGTTAGAGAGATGCAGCGGAAGAAGGAGAGCAGTGTCACAGAGTTCATCTCCAGGGAG
TGGCCAGACTCCCAGGCTCCGGTGACGGAGATCATGTCCCGACCAGAAAGGCTGTTGTTC
ATCATTGACGGTTTCGATGACCTGGGCTCTGTCCTCAACAATGACACAAAGCTCTGCAAA
GACTGGGCTGAGAAGCAGCCTCCGTTCACCCTCATACGCAGTCTGCTGAGGAAGGTCCTG
CTCCCTGAGTCCTTCCTGATCGTCACCGTCAGAGACGTGGGCACAGAGAAGCTCAAGTCA
GAGGTCGTGTCTCCCCGTTACCTGTTAGTTAGAGGAATCTCCGGGGAACAAAGAATCCAC
TTGCTCCTTGAGCGCGGGATTGGTGAGCATCAGAAGACACAAGGGTTGCGTGCGATCATG
AACAACCGTGAGCTGCTCGACCAGTGCCAGGTGCCCGCCGTGGGCTCTCTCATCTGCGTG
GCCCTGCAGCTGCAGGACGTGGTGGGGGAGAGCGTCGCCCCCTTCAACCAAACGCTCACA
GGCCTGCACGCCGCTTTTGTGTTTCATCAGCTCACCCCTCGAGGCGTGGTCCGGCGCTGT
CTCAATCTGGAGGAAAGAGTTGTCCTGAAGCGCTTCTGCCGTATGGCTGTGGAGGGAGTG
TGGAATAGGAAGTCAGTGTTTGACGGTGACGACCTCATGGTTCAAGGACTCGGGGAGTCT
GAGCTCCGTGCTCTGTTTCACATGAACATCCTTCTCCCAGACAGCCACTGTGAGGAGTAC
TACACCTTCTTCCACCTCAGTCTCCAGGACTTCTGTGCCGCCTTGTACTACGTGTTAGAG
GGCCTGGAAATCGAGCCAGCTCTCTGCCCTCTGTACGTTGAGAAGACAAAGAGGTCCATG
GAGCTTAAACAGGCAGGCTTCCATATCCACTCGCTTTGGATGAAGCGTTTCTTGTTTGGC
CTCGTGAGCGAAGACGTAAGGAGGCCACTGGAGGTCCTGCTGGGCTGTCCCGTTCCCCTG
GGGGTGAAGCAGAAGCTTCTGCACTGGGTCTCTCTGTTGGGTCAGCAGCCTAATGCCACC
ACCCCAGGAGACACCCTGGACGCCTTCCACTGTCTTTTCGAGACTCAAGACAAAGAGTTT
GTTCGCTTGGCATTAAACAGCTTCCAAGAAGTGTGGCTTCCGATTAACCAGAACCTGGAC
TTGATAGCATCTTCCTTCTGCCTCCAGCACTGTCCGTATTTGCGGAAAATTCGGGTGGAT
GTCAAAGGGATCTTCCCAAGAGATGAGTCCGCTGAGGCATGTCCTGTGGTCCCTCTATGG
ATGCGGGATAAGACCCTCATTGAGGAGCAGTGGGAAGATTTCTGCTCCATGCTTGGCACC
CACCCACACCTGCGGCAGCTGGACCTGGGCAGCAGCATCCTGACAGAGCGGGCCATGAAG
ACCCTGTGTGCCAAGCTGAGGCATCCCACCTGCAAGATACAGACCCTGATGTTTAGAAAT
GCACAGATTACCCCTGGTGTGCAGCACCTCTGGAGAATCGTCATGGCCAACCGTAACCTA
AGATCCCTCAACTTGGGAGGCACCCACCTGAAGGAAGAGGATGTAAGGATGGCGTGTGAA
GCCTTAAAACACCCAAAATGTTTGTTGGAGTCTTTGAGGCTGGATTGCTGTGGATTGACC
CATGCCTGTTACCTGAAGATCTCCCAAATCCTTACGACCTCCCCCAGCCTGAAATCTCTG
AGCCTGGCAGGAAACAAGGTGACAGACCAGGGAGTAATGCCTCTCAGTGATGCCTTGAGA
GTCTCCCAGTGCGCCCTGCAGAAGCTGATACTGGAGGACTGTGGCATCACAGCCACGGGT
TGCCAGAGTCTGGCCTCAGCCCTCGTCAGCAACCGGAGCTTGACACACCTGTGCCTATCC
AACAACAGCCTGGGGAACGAAGGTGTAAATCTACTGTGTCGATCCATGAGGCTTCCCCAC
TGTAGTCTGCAGAGGCTGATGCTGAATCAGTGCCACCTGGACACGGCTGGCTGTGGTTTT
CTTGCACTTGCGCTTATGGGTAACTCATGGCTGACGCACCTGAGCCTTAGCATGAACCCT
GTGGAAGACAATGGCGTGAAGCTTCTGTGCGAGGTCATGAGAGAACCATCTTGTCATCTC
CAGGACCTGGAGTTGGTAAAGTGTCATCTCACCGCCGCGTGCTGTGAGAGTCTGTCCTGT
GTGATCTCGAGGAGCAGACACCTGAAGAGCCTGGATCTCACGGACAATGCCCTGGGTGAC
GGTGGGGTTGCTGCGCTGTGCGAGGGACTGAAGCAAAAGAACAGTGTTCTGGCGAGACTC
GGGTTGAAGGCATGTGGACTGACTTCTGATTGCTGTGAGGCACTCTCCTTGGCCCTTTCC
TGCAACCGGCATCTGACCAGTCTAAACCTGGTGCAGAATAACTTCAGTCCCAAAGGAATG
ATGAAGCTGTGTTCGGCCTTTGCCTGTCCCACGTCTAACTTACAGATAATTGGGCTGTGG
AAATGGCAGTACCCTGTGCAAATAAGGAAGCTGCTGGAGGAAGTGCAGCTACTCAAGCCC
CGAGTCGTAATTGACGGTAGTTGGCATTCTTTTGATGAAGATGACCGGTACTGGTGGAAA
AAC
Protein sequence predicted based on nucleotide sequence
MKVAGGLELGAAALLSASPRALVTLSTGPTCSILPKNPLFPQNLSSQPCIKMEGDKSLTF SSYGLQWCLYELDKEEFQTFKELLKKKSSESTTCSIPQFEIENANVECLALLLHEYYGAS LAWATSISIFENMNLRTLSEKARDDMKRHSPEDPEATMTDQGPSKEKVPGISQAVQQDSA TAAETKEQEISQAMEQEGATAAETEEQGHGGDTWDYKSHVMTKFAEEEDVRRSFENTAAD WPEMQTLAGAFDSDRWGFRPRTVVLHGKSGIGKSALARRIVLCWAQGGLYQGMFSYVFFL PVREMQRKKESSVTEFISREWPDSQAPVTEIMSRPERLLFIIDGFDDLGSVLNNDTKLCK DWAEKQPPFTLIRSLLRKVLLPESFLIVTVRDVGTEKLKSEVVSPRYLLVRGISGEQRIH LLLERGIGEHQKTQGLRAIMNNRELLDQCQVPAVGSLICVALQLQDVVGESVAPFNQTLT GLHAAFVFHQLTPRGVVRRCLNLEERVVLKRFCRMAVEGVWNRKSVFDGDDLMVQGLGES ELRALFHMNILLPDSHCEEYYTFFHLSLQDFCAALYYVLEGLEIEPALCPLYVEKTKRSM ELKQAGFHIHSLWMKRFLFGLVSEDVRRPLEVLLGCPVPLGVKQKLLHWVSLLGQQPNAT TPGDTLDAFHCLFETQDKEFVRLALNSFQEVWLPINQNLDLIASSFCLQHCPYLRKIRVD VKGIFPRDESAEACPVVPLWMRDKTLIEEQWEDFCSMLGTHPHLRQLDLGSSILTERAMK TLCAKLRHPTCKIQTLMFRNAQITPGVQHLWRIVMANRNLRSLNLGGTHLKEEDVRMACE ALKHPKCLLESLRLDCCGLTHACYLKISQILTTSPSLKSLSLAGNKVTDQGVMPLSDALR VSQCALQKLILEDCGITATGCQSLASALVSNRSLTHLCLSNNSLGNEGVNLLCRSMRLPH CSLQRLMLNQCHLDTAGCGFLALALMGNSWLTHLSLSMNPVEDNGVKLLCEVMREPSCHL QDLELVKCHLTAACCESLSCVISRSRHLKSLDLTDNALGDGGVAALCEGLKQKNSVLARL GLKACGLTSDCCEALSLALSCNRHLTSLNLVQNNFSPKGMMKLCSAFACPTSNLQIIGLW KWQYPVQIRKLLEEVQLLKPRVVIDGSWHSFDEDDRYWWKN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3546
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
