Gene:
Gene Symbol:
SMC4
Gene Name:
structural maintenance of chromosomes 4
Original Clone ID:
FLH265086.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080465
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCCCGTAAAGGCACCCAGCCCTCCACTGCCCGGCGCAGAGAGGAAGGGCCGCCGCCG
CCGTCCCCTGACGGCGCCAGCAGCGACGCGGAGCCTGAGCCGCCGTCCGGCCGCACGGAG
AGCCCAGCCACCGCCGCAGAGACTGCAAGTGAGGAACTTGATAATAGAAGTTTAGAAGAG
ATTTTGAACAGCATTCCTCCTCCCCCGCCTCCAGCAATGACCAATGAAGCTGGAGCTCCT
CGGCTTATGATAACTCATATTGTAAACCAGAACTTCAAATCCTATGCTGGGGAGAAAATT
CTGGGACCTTTCCATAAGCGCTTTTCCTGTATTATCGGGCCAAATGGCAGTGGCAAATCC
AATGTTATTGATTCTATGCTTTTTGTGTTTGGCTATCGAGCACAAAAAATAAGATCTAAA
AAACTCTCAGTATTAATACATAATTCTGATGAACACAAGGACATTCAGAGTTGTACAGTA
GAAGTTCATTTTCAAAAGATAATTGATAAGGAAGGGGATGATTATGAAGTCATTCCTAAC
AGTAATTTCTATGTATCCAGAACGGCCTGCAGAGATAATACTTCTGTCTATCACATAAGT
GGAAAGAAAAAGACATTTAAGGATGTTGGAAATCTTCTTCGAAGCCATGGAATTGACTTG
GACCATAATAGATTTTTAATTTTACAGGGTGAAGTTGAACAAATTGCTATGATGAAACCA
AAAGGCCAGACTGAACACGATGAGGGTATGCTTGAATATTTAGAAGATATAATTGGTTGT
GGACGGCTAAATGAACCTATTAAAGTCTTGTGTCGGAGAGTTGAAATATTAAATGAACAC
AGAGGAGAGAAGTTAAACAGGGTAAAGATGGTGGAAAAGGAAAAGGATGCCTTAGAAGGA
GAGAAAAACATAGCTATCGAATTTCTTACCTTGGAAAATGAAATATTTAGAAAAAAGAAT
CATGTTTGTCAATATTATATTTATGAGTTGCAGAAACGAATTGCTGAAATGGAAACTCAA
AAGGAAAAAATTCATGAAGATACCAAAGAAATTAATGAGAAGAGCAATATACTATCAAAT
GAAATGAAAGCTAAGAATAAAGATGTAAAAGATACAGAAAAGAAACTGAATAAAATTACA
AAATTTATTGAGGAGAATAAAGAAAAATTTACACAGCTAGATTTGGAAGATGTTCAAGTT
AGAGAAAAGTTAAAACATGCCACGAGTAAAGCCAAAAAACTGGAGAAACAACTTCAAAAA
GATAAAGAAAAGGTTGAAGAATTTAAAAGTATACCTGCCAAGAGTAACAATATCATTAAT
GAAACAACAACCAGAAACAATGCCCTCGAGAAGGAAAAAGAGAAAGAAGAAAAAAAATTA
AAGGAAGTTATGGATAGCCTTAAACAGGAAACACAAGGGCTTCAGAAAGAAAAAGAAAGT
CGAGAGAAAGAACTTATGGGTTTCAGCAAATCGGTAAATGAAGCACGTTCAAAGATGGAT
GTAGCCCAGTCAGAACTTGATATCTATCTCAGTCGTCATAATACTGCAGTGTCTCAATTA
ACTAAGGCTAAGGAAGCTCTAATTGCAGCTTCTGAGACTCTCAAAGAAAGGAAAGCTGCA
ATCAGAGATATAGAAGGAAAACTCCCTCAAACTGAACAAGAATTAAAGGAGAAAGAAAAA
GAACTTCAAAAACTTACACAAGAAGAAACAAACTTTAAAAGTTTGGTTCATGATCTCTTT
CAAAAAGTTGAAGAAGCAAAGAGCTCATTAGCAATGAATCGAAGTAGGGGGAAAGTCCTT
GATGCAATAATTCAAGAAAAAAAATCTGGCAGGATTCCAGGAATATATGGAAGATTGGGG
GACTTAGGAGCCATTGATGAAAAATACGACGTGGCTATATCATCCTGTTGTCATGCACTG
GACTACATTGTTGTTGATTCTATTGATATAGCCCAAGAATGTGTAAACTTCCTTAAAAGA
CAAAATATTGGAGTTGCAACCTTTATAGGTTTAGATAAGATGGCTGTATGGGCGAAAAAG
ATGACCGAAATTCAAACTCCTGAAAATACTCCTCGTTTATTTGATTTAGTAAAAGTAAAA
GATGAGAAAATTCGCCAAGCTTTTTATTTTGCTTTACGAGATACCTTAGTAGCTGACAAC
TTGGATCAAGCCACAAGAGTAGCATATCAAAAAGATAGAAGATGGAGAGTGGTAACTTTA
CAGGGACAAATCATAGAACAGTCAGGTACAATGACTGGTGGTGGAAGCAAAGTAATGAAA
GGAAGAATGGGTTCCTCACTTGTTATTGAAATCTCTGAAGAAGAGGTAAACAAAATGGAA
TCACAGTTGCAAAACGACTCTAAAAAAGCAATGCAAATCCAAGAACAGAAAGTACAACTT
GAAGAAAGAGTAGTTAAGTTACGGCATAGTGAACGAGAAATGAGGAACACACTAGAAAAA
TTTACTGCAAGCATCCAGCGTTTAATAGAGCAAGAAGAATATTTGAATGTCCAAGTTAAG
GAACTTGAAGCTAATGTACTTGCTACAGCCCCTGACAAAAAAAAGCAGAAATTGCTAGAA
GAAAACGTTAGTGCTTTCAAAACAGAATATGATGCTGTGGCTGAGAAAGCTGGTAAAGTA
GAAGCTGAGGTTAAACGCTTACACAATACCATCGTAGAAATCAATAATCATAAACTCAAG
GCCCAACAAGACAAACTTGATAAAATAAATAAGCAATTAGATGAATGTGCTTCTGCTATT
ACTAAAGCCCAAGTAGCAATCAAGACTGCTGACAGAAACCTTCAAAAGGCACAAGACTCT
GTCTTGCGTACAGAGAAAGAAATAAAAGATACTGAGAAAGAGGTGGATGACCTAACAGCA
GAGCTGAAAAGTCTTGAGGACAAAGCAGCAGAGGTCGTAAAGAATACAAATGCTGCAGAG
GAATCCTTACCAGAGATCCAGAAAGAACATCGCAATCTGCTTCAAGAATTAAAAGTTATT
CAAGAAAATGAACATGCTCTTCAAAAAGATGCACTTAGTATTAAGTTGAAACTTGAACAA
ATAGATGGTCACATTGCTGAACATAATTCTAAAATAAAATATTGGCACAAAGAGATTTCA
AAAATATCACTGCATCCTATAGAAGATAATCCTATTGAAGAGATTTCGGTTCTAAGCCCA
GAGGATCTTGAAGCGATCAAGAATCCAGATTCTATAACAAATCAAATTGCACTTTTGGAA
GCCCGGTGTCATGAAATGAAACCAAACCTCGGTGCCATCGCAGAGTATAAAAAGAAGGAA
GAATTGTATTTGCAACGGGTAGCAGAATTGGACAAAATTACTTATGAAAGAGACAGTTTT
AGACAGGCATATGAAGATCTTCGGAAACAAAGGCTTAATGAATTTATGGCAGGTTTTTAT
ATAATAACAAATAAATTAAAGGAAAATTACCAAATGCTTACTTTGGGAGGGGACGCCGAA
CTCGAGCTTGTAGACAGCTTGGATCCTTTCTCTGAAGGAATCATGTTCAGTGTTCGACCA
CCTAAGAAAAGTTGGAAAAAGATCTTCAACCTTTCGGGAGGAGAGAAAACACTTAGTTCA
TTGGCTTTAGTATTTGCTCTTCACCACTACAAGCCCACTCCCCTTTACTTCATGGATGAG
ATTGATGCAGCCCTTGATTTTAAAAATGTGTCCATTGTTGCATTTTATATATATGAACAA
ACAAAAAATGCACAGTTCATAATAATTTCTCTTCGAAATAATATGTTTGAGATTTCGGAT
AGACTTATTGGAATTTACAAGACATACAACATAACAAAAAGTGTTGCTGTAAATCCAAAA
GAAATTGCATCTAAGGGACTTTGT
Protein sequence predicted based on nucleotide sequence
MPRKGTQPSTARRREEGPPPPSPDGASSDAEPEPPSGRTESPATAAETASEELDNRSLEE ILNSIPPPPPPAMTNEAGAPRLMITHIVNQNFKSYAGEKILGPFHKRFSCIIGPNGSGKS NVIDSMLFVFGYRAQKIRSKKLSVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPN SNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKP KGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEG EKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSN EMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQK DKEKVEEFKSIPAKSNNIINETTTRNNALEKEKEKEEKKLKEVMDSLKQETQGLQKEKES REKELMGFSKSVNEARSKMDVAQSELDIYLSRHNTAVSQLTKAKEALIAASETLKERKAA IRDIEGKLPQTEQELKEKEKELQKLTQEETNFKSLVHDLFQKVEEAKSSLAMNRSRGKVL DAIIQEKKSGRIPGIYGRLGDLGAIDEKYDVAISSCCHALDYIVVDSIDIAQECVNFLKR QNIGVATFIGLDKMAVWAKKMTEIQTPENTPRLFDLVKVKDEKIRQAFYFALRDTLVADN LDQATRVAYQKDRRWRVVTLQGQIIEQSGTMTGGGSKVMKGRMGSSLVIEISEEEVNKME SQLQNDSKKAMQIQEQKVQLEERVVKLRHSEREMRNTLEKFTASIQRLIEQEEYLNVQVK ELEANVLATAPDKKKQKLLEENVSAFKTEYDAVAEKAGKVEAEVKRLHNTIVEINNHKLK AQQDKLDKINKQLDECASAITKAQVAIKTADRNLQKAQDSVLRTEKEIKDTEKEVDDLTA ELKSLEDKAAEVVKNTNAAEESLPEIQKEHRNLLQELKVIQENEHALQKDALSIKLKLEQ IDGHIAEHNSKIKYWHKEISKISLHPIEDNPIEEISVLSPEDLEAIKNPDSITNQIALLE ARCHEMKPNLGAIAEYKKKEELYLQRVAELDKITYERDSFRQAYEDLRKQRLNEFMAGFY IITNKLKENYQMLTLGGDAELELVDSLDPFSEGIMFSVRPPKKSWKKIFNLSGGEKTLSS LALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQTKNAQFIIISLRNNMFEISD RLIGIYKTYNITKSVAVNPKEIASKGLC
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
269
End on reference sequence
4135
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
