SLC5A4 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SLC5A4
Gene Name:  solute carrier family 5 (glucose activated ion channel), member 4
Original Clone ID:
FLH265093.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080472
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1977nts
Open reading frame: 1 to 1977

ATGGCCAGTACGGTTAGCCCCAGCACCATAGCTGAGACCCCAGAGCCACCTCCATTGTCT
GACCACATCCGAAATGCTGCTGACATCTCAGTCATTGTCATCTATTTTCTGGTGGTGATG
GCTGTTGGGCTGTGGGCGATGCTGAAGACCAACCGAGGTACTATAGGAGGCTTCTTCCTC
GCTGGTCGTGATATGGCCTGGTGGCCGATGGGCGCCTCTCTCTTTGCCAGTAACATCGGC
AGCAACCACTATGTGGGGCTGGCTGGGACAGGAGCAGCTTCAGGAGTCGCCACCGTAACA
TTTGAATGGACTTCCTCAGTAATGTTGCTGATTCTTGGGTGGATCTTTGTCCCTATCTAC
ATCAAGTCGGGGGTGATGACCATGCCGGAATATCTCAAGAAGCGGTTTGGTGGGGAGCGA
CTCCAGGTCTACCTCTCCATCCTCTCCCTCTTCATCTGTGTGGTTTTGTTAATTTCTGCA
GACATATTTGCTGGAGCCATATTCATCAAGCTGGCCTTGGGATTGGACCTTTACCTGGCA
ATCTTCATCCTCTTGGCTATGACTGCTGTTTACACCACCACTGGGGGCTTGGCCTCGGTG
ATTTACACAGACACCCTCCAGACCATCATCATGCTGATTGGCTCTTTTATTCTCATGGGG
TTTGCATTTAACGAAGTTGGAGGTTATGAGAGCTTTACCGAGAAGTACGTGAATGCCACC
CCATCCGTAGTCGAGGGGGACAACTTGACAATCAGTGCCAGTTGCTACACACCTCGGGCG
GACTCCTTCCATATCTTCCGAGATGCTGTGACTGGGGACATTCCATGGCCAGGAATTATA
TTTGGAATGCCCATTACAGCTTTGTGGTACTGGTGCACAAATCAGGTCATTGTGCAGCGC
TGCCTGTGTGGCAAGGACATGTCTCACGTGAAGGCCGCTTGCATTATGTGTGCTTACCTG
AAGCTGCTGCCCATGTTCCTCATGGTGATGCCGGGGATGATCAGCCGCATCCTGTACACA
GATATGGTAGCATGTGTGGTACCTTCTGAATGCGTGAAACACTGTGGCGTTGATGTTGGC
TGCACCAACTACGCATACCCCACGATGGTGCTGGAACTGATGCCCCAAGGACTGCGAGGC
CTGATGCTTTCGGTCATGCTGGCCTCTCTCATGAGCTCCCTGACCTCCATCTTCAACAGC
GCCAGCACCCTCTTCACCATTGACCTCTACACCAAGATGCGGAAGCAAGCGTCGGAGAAA
GAGCTCCTGATAGCTGGACGGATATTTGTTCTTCTATTAACTGTTGTGAGCATTGTGTGG
GTCCCACTGGTACAAGTTTCTCAAAATGGACAACTAATCCATTACACAGAATCAATTTCT
AGCTACCTTGGGCCTCCAATTGCAGCTGTCTTTGTGCTTGCCATCTTCTGTAAAAGAGTC
AATGAACAGGGAGCATTCTGGGGTCTAATGGTTGGACTTGCAATGGGCCTCATTCGTATG
ATAACAGAGTTTGCTTATGGAACAGGGAGTTGCTTGGCTCCCAGTAACTGTCCCAAGATT
ATCTGTGGAGTGCACTATCTGTACTTTTCCATCGTTCTCTTTTTTGGGTCCATGCTGGTC
ACCCTGGGAATTTCCCTCTTAACAAAACCCATTCCTGATGTACATCTGTACCGCCTGTGC
TGGGTTCTTCGGAACAGTACAGAGGAGCGAATCGATATAGATGCAGAAGAGAAAAGTCAG
GAAGAAACAGATGATGGTGTTGAAGAAGATTATCCTGAGAAATCACGTGGATGCCTCAAG
AAAGCTTATGACTTGTTCTGCGGTTTGCAGAAGGGACCCAAGCTAACCAAGGAGGAGGAG
GAAGCCTTGAGCAAGAAGCTCACAGACACGTCTGAGAGGCCCTCGTGGAGGACAATAGTG
AACATCAACGCCATCCTCCTCCTGGCTGTGGTGGTCTTTATTCACGGCTACTATGCC

Protein sequence predicted based on nucleotide sequence

MASTVSPSTIAETPEPPPLSDHIRNAADISVIVIYFLVVMAVGLWAMLKTNRGTIGGFFL
AGRDMAWWPMGASLFASNIGSNHYVGLAGTGAASGVATVTFEWTSSVMLLILGWIFVPIY
IKSGVMTMPEYLKKRFGGERLQVYLSILSLFICVVLLISADIFAGAIFIKLALGLDLYLA
IFILLAMTAVYTTTGGLASVIYTDTLQTIIMLIGSFILMGFAFNEVGGYESFTEKYVNAT
PSVVEGDNLTISASCYTPRADSFHIFRDAVTGDIPWPGIIFGMPITALWYWCTNQVIVQR
CLCGKDMSHVKAACIMCAYLKLLPMFLMVMPGMISRILYTDMVACVVPSECVKHCGVDVG
CTNYAYPTMVLELMPQGLRGLMLSVMLASLMSSLTSIFNSASTLFTIDLYTKMRKQASEK
ELLIAGRIFVLLLTVVSIVWVPLVQVSQNGQLIHYTESISSYLGPPIAAVFVLAIFCKRV
NEQGAFWGLMVGLAMGLIRMITEFAYGTGSCLAPSNCPKIICGVHYLYFSIVLFFGSMLV
TLGISLLTKPIPDVHLYRLCWVLRNSTEERIDIDAEEKSQEETDDGVEEDYPEKSRGCLK
KAYDLFCGLQKGPKLTKEEEEALSKKLTDTSERPSWRTIVNINAILLLAVVVFIHGYYA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 17
End on reference sequence 1996
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable