Gene:
Gene Symbol:
NRAP
Gene Name:
nebulin-related anchoring protein
Original Clone ID:
FLH265119.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR/D-TOPO
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080498
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAATGTGCAGCCCTGTTCTAGGTGTGGGTATGGGGTTTATCCTGCCGAGAAGATCAGC
TGTATAGATCAGATATGGCATAAAGCCTGTTTTCACTGTGAAGTTTGCAAGATGATGCTG
TCTGTTAATAACTTTGTGAGTCACCAGAAAAAGCCGTACTGTCACGCCCATAACCCTAAG
AACAACACTTTCACCAGTGTCTATCACACTCCATTAAATCTAAATGTGAGGACATTTCCA
GAGGCCATCAGTGGGATCCATGACCAAGAAGATGGTGAACAGTGTAAATCAGTTTTTCAT
TGGGACATGAAATCCAAGGATAAGGAAGGTGCACCTAACAGGCAGCCACTGGCAAATGAG
AGAGCCTATTGGACTGGATATGGGGAAGGGAATGCTTGGTGCCCAGGAGCTCTGCCAGAC
CCCGAAATTGTAAGGATGGTTGAGGCTCGAAAGTCTCTTGGTGAGGAATATACAGAAGAC
TATGAGCAACCCAGGGGCAAGGGGAGCTTTCCAGCCATGATCACACCTGCTTATCAAAGG
GCCAAGAAAGCCAACCAGCTGGCCAGCCAAGTGGAGTATAAGAGAGGGCATGATGAACGC
ATCTCCAGGTTCTCCACGGTGGCGGATACTCCTGAGCTGCTACGGAGCAAGGCTGGGGCA
CAGCTTCAAAGTGATGTGAGATACACAGAGGACTATGAACAACAAAGAGGGAAAGGCAGT
TTCCCTGCGATGATCACACCCGCCTGTCAGATAGCCAAAAGAGCCAATGAGCTGGCAAGT
GATGTGAGGTACCATCAACAATATCAAAAAGAAATGAGGGGAATGGCTGGTCCAGCCATT
GGAGCTGAGGGCATCTTGACAAGGGAATGTGCAGACCAATATGGCCAGGGTTACCCGGAG
GAGTATGAGGAGCACAGGGGAAAGGGCAGCTTCCCAGCTATGATCACTCCAGCATATCAG
AACGCCAAGAAAGCTCACGAACTCGCTAGTGACATAAAATACAGGCAGGACTTCAATAAG
ATGAAAGGCACTGCACATTATCACTCGCTTCCAGCTCAAGACAACTTGGTTCTCAAACGG
GCTCAGAGCGTAAACAAACTCGTGAGTGAGGTGGAGTATAAGAAGGATCTGGAAAGTAGT
AGAGGTCACAGTATCAACTACTGTGAAACACCTCAATTCAGGAACGTGAGCAAGATCTCA
AAATTTACCAGTGATAATAAATATAAAGAAAACTACCAGAACCACATGAGAGGCCGCTAT
GAAGGAGTTGGTATGGACAGACGCACTCTGCATGCTATGAAAGTTGGCAGCCTGGCAAGC
AACGTTGCCTACAAAGCTGATTATAAACATGATATTGTCGACTACAACTACCCAGCCACT
CTCACGCCTTCCTATCAAACAGCTATGAAACTGGTGCCCTTGAAAGATGCCAATTATAGG
CAGAGCATCGACAAGTTGAAGTACAGCTTGGTGACTGACACCCCACAGATTGTTCAAGCC
AAAATCAATGCCCAGCAGCTGAGTCATGTGAATTACCGTGCTGACTATGAGAAAAATAAG
TTGAATTACACATTGCCCCAGGATGTTCCTCAGCTGGTGAAGGCCAAAACCAATGCCAAA
CTCTTCAGTGAGGTTAAGTATAAAGAAGGCTGGGAGAAGACAAAGGGGAAAGGATTTGAG
ATGAAGCTGGATGCCATGTCTCTGCTGGCCGCCAAAGCCTCTGGGGAGCTTGCTAGCAAT
ATTAAATATAAAGAAGAATATGAAAAGACAAAAGGCAAAGCCATGGGAACAGCCGACTCT
AGGCTTCTGCACTCCCTGCAGATTGCTAAGATGAGCAGTGAGGTTGAATATAAGAAAGGC
TTTGAAGAGAGTAAGACCCGGTTTCACCTGCCCATGGATATGGTAAACATCAGGCATGCT
AAGAAGGCCCAAACTCTCGCCAGTGACCTGGACTACAGGAAGAAACTGCATGAATACACT
GTGCTGCCTGAAGATATGAAGACTCAGTGGGCCAAGAAGGCCTATGGGCTCCAGAGCGAG
CTGCAGTACAAGGCTGACCTGGCATGGATGAAGGGAGTTGGGTGGCTGACAGAGGGGAGT
CTCAACTTGGAACAAGCCAAGAAGGCTGGACAGCTGGTCAGCGAGAAAAACTACCGGCAG
AGGGTCGATGAGCTGAAGTTCACCAGCGTGACCGACAGCTCCCAGATGGAGCACGCCAAG
AAGAGCCAGGAGCTACAGAGCGGGGTGGCCTACAAGGCAGGAAATGAGCAGTCTGTCCAT
CAGTATACCATCAGCAAAGACGAGCCTCTCTTCCTGCAGGCCCGAGCCAATGCTGCAAAT
CTCAGCGAGAAACTGTATAAGAGCAGCTGGGAAAACCAGAAAGCAAAAGGGTTTGAGCTG
CGTCTTGATTCCTTGACCTTCCTGGCAGCCAAAGCCAAGAGGGACCTGGCTAGCGAGGTG
AAGTACAAGGAGGATTATGAGAGATCCAGAGGGAAGCTCATTGGGGCAAAAGATGTACAG
GGAGATTCGCAAATGAGCCACTCACTGCAAATGTCCAAGCTGCAGAGTGAGCTGGAGTAC
AAGAAGGGATTCGAGGACACCAAATCCCAATGCCACGTCTCCCTGGACATGGTCCACCTC
GTGCATGCCCGCAAAGCTCAGCATTTAGCCACAGACGTAGGCTACAAGACAGCGGAACAT
CACTTTACGGCTTTGCCCACAGACATGAAGGTGGAATGGGCCAAGAAGGCTTATGGCTTA
CAGAGTGATAACCAATACAGGGCAGATGTGAAGTGGATGAAAGGCATGGGCTGGGTCGCC
ACCGGGTCATTAAATGTGGAGCAGGCGAAGAAGGCAGGAGAACTCATTAGCGAGAAGAAG
TACCGTCAGCATCCAGATGCTTTGAAGTTTACCAGTATTAAAGACACTCCGGAGATGGTC
CAGGCCAGAATTAGTTATACCCAAGCAGTGGATAGATTATACAGGGAACAAGGAGAAAAC
ATAAAGCATCATTACACACCTACTGCTGACCTCCCTGAAGTCCTGCTGGCCAAGCTGAAT
GCCATGAATATCAGTGAGACGCGTTATAAGGAATCCTGGAGCAAACTTCGAGATGGTGGC
TATAAACTGAGGTTGGATGCCCTTCCATTCCAAGCAGCAAAGGCTTCTGGTGAAATCATA
AGTGATTACAAATACAAAGAGGCATTTGAGAAAATGAAAGGACAGATGCTTGGTTCCCGG
AGCTTGGAAGATGATATCAGCCTTGCACATTCAGTGTATGCGACCTCACTGCAGAGTGAT
GTGAATTACAAGAAAGGCTTTGAACACTCAAAGGCGCAGTTCCATCTGCCGTTGGACATG
GCCGCCCTGGTGCATGCCAAGAAGGCTCAGACCCTGGCCAGCAATCAGGACTACAAACAT
CCACTGCCCCAGTACACTTCCTTGGCAGAAGACCTGAGGCTGAGCTGTGCCAAGAAAGCT
CACAAATTGCAGAGTGAGAATTTGTACCGGTCAGACCTGAACTTTATGCGAGGTGTTGCA
TGTGTCGTTCCAGGCACGTTAGAGATCGAAGGGAGGAAGAAAGCATCGGAACTCATCAGT
GAGAGTAAATATCGGCAGCATCCCCACTCATTCAAGTACACAGCTGTGACAGACACTCCC
AACCTCCTTCATGCGAAATTCAGCAACCAGATAACGAATGAGCGCCTCTATAAAGCAGCT
GGAGAGGATGCAAGACACGAGTATACAATGACCCTGGGTCTGCCCGAGTTCATCCGAGCA
AAAACGAATGCAGCCAACCTGAGTGACGCAAGATACAAAGAGTCCTGGCGTAATCTTCGT
GCTCAAGGCTACAAGCTGACAATAGAAGCGCTCCCCTTCCAGGCTGCCCGGGCCTCTGGA
GATATAGCCAGTGATTTTCTCTACAGACATGACTTTGTGAAGGAGCGAGGGAAACTCATA
GGGCCCCAGAGTGTAAGAGACGACCCCCGGATCCAGCACTGCCGGCGCATGGGCCAGCTG
CAGAGCGAGCTTCAGTACAGGAGGGGGGCGACCAGCAGCCAAGCCCAGCTCCATCTGCCC
ATGGACATGGTGCACCTGGTCCATGCCAAGAATGCCCAGGCTCTGGCCAGCGACCACGAC
TACAGGACACAGTATCACAAGTTCACAGCACTGCCCGAGGACCTGAAGATGGCCTGGGCC
AAGAAAGCCCATGCCCTGCAGAGTGAGTTGCGCTACAAGTCAGACCTGATCGGCATGAAG
GGCATAGGATGGCTGGCGCTGAGATCCCCACAGATGGAGAGTGCAAAGAAGGCTGGAGAA
CTCATCAGCGAGACCAAGTACCGTAAAAAACCAGACAGTATCAAGTTCACCACAGTGGTT
GACTCCCCAGACCTGGTTCATGCCAAGAACAGCTATATGCACTGCAATGAGCGCATGTAT
AGATCTGGAGATGCAGAATCCCTGCACAGATACACCCTGATCCCCGACCATCCCGATTTC
ACCCGAGCTCGCCTCAATGCGCTGCATCTGAGTGACAAAGTCTACAGAAACTCCTGGGAG
CAGACCCGGGCTGGCAGTTATGACTTCAGGCTGGATGCCATCCCCTTCCAGACTGCCCGG
GCATCTAGGGAGATCGCCAGTGATTTCCGGTACAAAGAGGCTTTCCTGCGGGACCGAGGC
CTGCAGATCGGGTACCGCAGTGTCGACGATGACCCAAGGATGAAGCATTTCCTCAACGTT
GGCAGGCTCCAGAGTGACAATGAGTACAAGAAGGACTTTGCCAAGAGTCGGTCCCAGTTT
CACAGCAGCACAGACCAGCCCGGCCTCCTTCAGGCCAAGAGGAGCCAGCAGCTGGCCAGT
GATGTGCACTACAGGCAGCCCCTGCCCCAGCCCACCTGCGACCCGGAGCAGCTGGGCCTC
AGGCATGCTCAGAAGGCCCACCAGCTGCAGAGTGATGTCAAGTATAAATCAGACTTGAAC
CTGACCAGAGGTGTTGGCTGGACCCCTCCTGGCTCCTACAAAGTGGAAATGGCTCGGCGG
GCTGCGGAACTGGCCAACGCAAGGGGCCTGGGTCTCCAGGGAGCTTACCAGGGGGCAGAA
GCAGTGGAGGCTGGAGATCATCAGAGTGGGGAGGTGAACCCAGATGCCACTGAGATTCTG
CACGTCAAAAAGAAGAAGGCCCTGCTGTTG
Protein sequence predicted based on nucleotide sequence
MNVQPCSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPK NNTFTSVYHTPLNLNVRTFPEAISGIHDQEDGEQCKSVFHWDMKSKDKEGAPNRQPLANE RAYWTGYGEGNAWCPGALPDPEIVRMVEARKSLGEEYTEDYEQPRGKGSFPAMITPAYQR AKKANQLASQVEYKRGHDERISRFSTVADTPELLRSKAGAQLQSDVRYTEDYEQQRGKGS FPAMITPACQIAKRANELASDVRYHQQYQKEMRGMAGPAIGAEGILTRECADQYGQGYPE EYEEHRGKGSFPAMITPAYQNAKKAHELASDIKYRQDFNKMKGTAHYHSLPAQDNLVLKR AQSVNKLVSEVEYKKDLESSRGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGRY EGVGMDRRTLHAMKVGSLASNVAYKADYKHDIVDYNYPATLTPSYQTAMKLVPLKDANYR QSIDKLKYSLVTDTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAK LFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTADS RLLHSLQIAKMSSEVEYKKGFEESKTRFHLPMDMVNIRHAKKAQTLASDLDYRKKLHEYT VLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQ RVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYTISKDEPLFLQARANAAN LSEKLYKSSWENQKAKGFELRLDSLTFLAAKAKRDLASEVKYKEDYERSRGKLIGAKDVQ GDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMVHLVHARKAQHLATDVGYKTAEH HFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGMGWVATGSLNVEQAKKAGELISEKK YRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTPTADLPEVLLAKLN AMNISETRYKESWSKLRDGGYKLRLDALPFQAAKASGEIISDYKYKEAFEKMKGQMLGSR SLEDDISLAHSVYATSLQSDVNYKKGFEHSKAQFHLPLDMAALVHAKKAQTLASNQDYKH PLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVACVVPGTLEIEGRKKASELIS ESKYRQHPHSFKYTAVTDTPNLLHAKFSNQITNERLYKAAGEDARHEYTMTLGLPEFIRA KTNAANLSDARYKESWRNLRAQGYKLTIEALPFQAARASGDIASDFLYRHDFVKERGKLI GPQSVRDDPRIQHCRRMGQLQSELQYRRGATSSQAQLHLPMDMVHLVHAKNAQALASDHD YRTQYHKFTALPEDLKMAWAKKAHALQSELRYKSDLIGMKGIGWLALRSPQMESAKKAGE LISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERMYRSGDAESLHRYTLIPDHPDF TRARLNALHLSDKVYRNSWEQTRAGSYDFRLDAIPFQTARASREIASDFRYKEAFLRDRG LQIGYRSVDDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSSTDQPGLLQAKRSQQLAS DVHYRQPLPQPTCDPEQLGLRHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARR AAELANARGLGLQGAYQGAEAVEAGDHQSGEVNPDATEILHVKKKKALLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
116
End on reference sequence
5308
Mutations:
None
Discrepancy:
t1313c,L438P
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR/D-TOPO
Synonyms:
pENTR-D-TOPO
Description:
Gateway type (lambda att-type) recombinational cloning system entry (master) vector for TOPO cloning of insert; kanamycin resistance; recombinational cloning, TOPO cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://www.orfeomecollaboration.org/html/vectors.shtml.
Size (bp):
2580
Parent Vector:
None
Properties:
Gateway, TOPO Cloning, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (NotI and AscI sites flank the TOPO cloning site)
680
693
TOPO recognition site
TOPO 1
TOPO recognition site 1
680
684
TOPO recognition site
TOPO 2
TOPO recognition site 2
689
693
bacterial origin
pUC ori
pUC-type origin of replication
1904
2577
primer site
M13 F
M13 forward priming site
537
552
primer site
M13 Rev
M13 reverse primer site
845
861
promoter
T7
T7promoter and primer binding site
821
840
recombination site
attL1
attL1 recombination site
569
668
recombination site
attL2
attL2 recombination site
705
804
selectable marker
KanR
kanamycin resistance gene
974
1783
trxn termination sequence
rrnB trxn term
rrnB T2 transcription termination sequence
268
295
trxn termination sequence
rrnB txn term
rrnB T1 transcription termination sequence
427
470
