SEMA4F (Homo sapiens) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SEMA4F
Gene Name:  sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F
Original Clone ID:
FLH265173.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080552
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2310nts
Open reading frame: 1 to 2310

ATGCCGGCCTCTGCTGCGCGGCCCCGCCCGGGTCCCGGGCAGCCTACAGCCTCGCCCTTC
CCGCTACTGCTGCTGGCGGTGCTGAGCGGCCCGGTATCCGGCCGCGTCCCCCGCTCGGTG
CCCAGAACCTCGCTTCCAATCTCTGAGGCTGACTCCTGTCTCACCCGGTTCGCAGTCCCT
CACACATACAATTACTCTGTTCTCCTTGTGGATCCTGCCTCCCACACACTTTATGTTGGC
GCCCGGGACACCATCTTCGCTTTATCCCTGCCCTTCTCAGGGGAGAGACCCCGCAGGATT
GACTGGATGGTTCCTGAGGCTCACAGACAGAACTGTAGGAAGAAAGGCAAGAAAGAGGAC
GAATGTCACAATTTTGTCCAGATTCTCGCCATTGCCAATGCCTCTCACCTCCTCACTTGT
GGCACCTTCGCTTTTGATCCGAAGTGCGGGGTTATTGATGTGTCCAGGTTCCAGCAGGTT
GAAAGACTTGAGAGTGGCCGGGGGAAATGTCCTTTTGAGCCAGCTCAGCGGTCAGCAGCT
GTAATGGCTGGGGGGGTCCTCTATGCTGCCACTGTGAAAAACTACCTGGGGACGGAGCCA
ATTATCACCAGAGCAGTGGGTCGTGCCGAGGACTGGATTCGGACAGATACCTTGCCTTCC
TGGCTGAACGCCCCAGCCTTTGTCGCAGCCGTGGCCTTGAGCCCAGCCGAATGGGGGGAT
GAAGATGGAGACGACGAAATCTACTTCTTCTTTACGGAGACTTCCCGAGCATTTGACTCA
TACGAGCGCATTAAAGTCCCACGGGTGGCCCGTGTGTGTGCGGGGGACCTCGGGGGCCGG
AAGACCCTCCAGCAGAGATGGACGACGTTTTTGAAAGCTGACCTGCTCTGTCCAGGGCCT
GAGCATGGCCGGGCCTCCAGTGTCCTGCAGGATGTTGCTGTGCTTCGACCTGAGCTTGGG
GCAGGGACTCCCATCTTTTATGGCATCTTTTCTTCCCAGTGGGAGGGGGCTACTATCTCT
GCTGTCTGTGCCTTCCGACCACAAGACATTCGGACAGTGCTGAATGGTCCCTTCAGAGAA
CTAAAACATGACTGCAACAGAGGACTGCCTGTCGTGGACAATGATGTGCCCCAGCCCAGA
CCTGGAGAGTGCATCACCAACAACATGAAGCTCCGGCACTTTGGCTCATCTCTCTCCCTG
CCTGACCGCGTACTCACCTTCATCCGGGACCACCCACTCATGGACAGGCCAGTGTTTCCA
GCTGATGGCCACCCCCTGCTGGTCACTACAGATACAGCCTATCTCAGAGTCGTGGCCCAC
AGGGTGACCAGCCTCTCAGGGAAAGAGTATGATGTGCTCTACCTGGGGACAGAGGATGGA
CACCTCCACCGAGCAGTGCGGATCGGAGCTCAGCTCAGCGTTCTTGAAGATCTGGCCTTA
TTCCCAGAGCCACAGCCAGTTGAGAACATGAAATTGTACCACAGCTGGCTCCTGGTTGGC
TCCCGTACTGAGGTGACACAAGTGAATACAACCAACTGTGGCCGTCTCCAGAGCTGCTCA
GAGTGCATCCTGGCCCAGGACCCAGTCTGTGCCTGGAGCTTCCGGCTGGATGAGTGTGTG
GCCCATGCCGGGGAGCACCGAGGGTTGGTCCAAGACATAGAGTCAGCAGATGTCTCCTCT
TTGTGTCCTAAAGAGCCTGGAGAACGTCCAGTAGTGTTTGAAGTTCCCGTGGCTACAGCT
GCGCATGTGGTCTTGCCATGTTCTCCAAGCTCAGCATGGGCATCCTGTGTGTGGCACCAG
CCCAGTGGAGTGACTGCACTCACCCCCCGGCGGGATGGACTGGAGGTGGTGGTGACCCCA
GGGGCCATGGGCGCTTATGCCTGTGAATGTCAGGAGGGTGGGGCAGCCCATGTGGTAGCA
GCTTACAGCTTGGTATGGGGCAGCCAGCGAGATGCTCCGAGCCGGGCCCACACAGTGGGG
GCGGGACTGGCTGGCTTCTTCTTGGGGATTCTCGCAGCATCCCTGACTCTCATTCTGATT
GGTCGGCGTCAGCAGCGACGGCGACAGAGGGAACTTCTGGCTAGAGACAAGGTGGGCCTG
GACCTGGGGGCTCCACCTTCTGGGACCACAAGCTACAGCCAAGACCCTCCCTCCCCCTCT
CCTGAAGATGAGCGGTTGCCGCTGGCCCTGGCCAAGAGGGGCAGTGGCTTTGGTGGATTC
TCACCACCCTTCCTGCTTGATCCTTGCCCAAGCCCAGCCCACATTCGGCTAACTGGGGCT
CCTCTAGCCACATGTGATGAAACATCCATC

Protein sequence predicted based on nucleotide sequence

MPASAARPRPGPGQPTASPFPLLLLAVLSGPVSGRVPRSVPRTSLPISEADSCLTRFAVP
HTYNYSVLLVDPASHTLYVGARDTIFALSLPFSGERPRRIDWMVPEAHRQNCRKKGKKED
ECHNFVQILAIANASHLLTCGTFAFDPKCGVIDVSRFQQVERLESGRGKCPFEPAQRSAA
VMAGGVLYAATVKNYLGTEPIITRAVGRAEDWIRTDTLPSWLNAPAFVAAVALSPAEWGD
EDGDDEIYFFFTETSRAFDSYERIKVPRVARVCAGDLGGRKTLQQRWTTFLKADLLCPGP
EHGRASSVLQDVAVLRPELGAGTPIFYGIFSSQWEGATISAVCAFRPQDIRTVLNGPFRE
LKHDCNRGLPVVDNDVPQPRPGECITNNMKLRHFGSSLSLPDRVLTFIRDHPLMDRPVFP
ADGHPLLVTTDTAYLRVVAHRVTSLSGKEYDVLYLGTEDGHLHRAVRIGAQLSVLEDLAL
FPEPQPVENMKLYHSWLLVGSRTEVTQVNTTNCGRLQSCSECILAQDPVCAWSFRLDECV
AHAGEHRGLVQDIESADVSSLCPKEPGERPVVFEVPVATAAHVVLPCSPSSAWASCVWHQ
PSGVTALTPRRDGLEVVVTPGAMGAYACECQEGGAAHVVAAYSLVWGSQRDAPSRAHTVG
AGLAGFFLGILAASLTLILIGRRQQRRRQRELLARDKVGLDLGAPPSGTTSYSQDPPSPS
PEDERLPLALAKRGSGFGGFSPPFLLDPCPSPAHIRLTGAPLATCDETSI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 112
End on reference sequence 2424
Mutations:
None
Discrepancy:
a350g,K117R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable