Gene:
Gene Symbol:
Ephb2
Gene Name:
Eph receptor B2
Original Clone ID:
FLH265293.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080672
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2958nts
Open reading frame: 1 to 2958
ATGGCCGTGCGCAGGCTGGGGGCCGCGCTGCTGCTGCTGCCGCTGCTAGCCGCCGTGGAA
GAAACCCTGATGGACTCTACGACAGCAACGGCTGAGCTGGGCTGGATGGTACATCCCCCA
TCAGGGTGGGAAGAGGTGAGCGGCTACGACGAGAACATGAACACTATCCGTACCTACCAG
GTGTGCAATGTCTTTGAGTCAAGCCAGAACAACTGGCTGCGGACCAAATTCATCCGGCGC
CGCGGCGCCCACCGCATCCACGTGGAGATGAAGTTCTCGGTGCGTGACTGCAGCAGCATT
CCCAGCGTGCCGGGCTCCTGCAAGGAGACCTTCAACCTCTACTACTATGAGGCTGATTTT
GACTTAGCCACCAAAACCTTTCCCAACTGGATGGAGAATCCGTGGGTGAAGGTGGACACC
ATCGCGGCCGATGAGAGCTTCTCTCAGGTGGACCTGGGTGGCCGCGTCATGAAAATCAAC
ACTGAGGTGCGAAGCTTCGGTCCCGTGTCCCGCAATGGTTTCTACCTGGCCTTCCAGGAC
TATGGCGGCTGTATGTCCCTCATTGCTGTGCGCGTCTTCTACCGGAAGTGCCCCCGAATC
ATCCAGAATGGTGCCATCTTCCAGGAGACACTGTCGGGGGCTGAGAGCACTTCGCTGGTG
GCAGCTCGGGGCAGCTGCATCGCCAATGCTGAAGAAGTGGATGTGCCCATCAAACTCTAC
TGTAACGGGGACGGCGAATGGCTGGTGCCCATAGGTCGCTGCATGTGCAAGGCGGGCTTC
GAGGCTGTGGAGAACGGCACCGTCTGCCGAGGTTGTCCATCAGGAACCTTCAAGGCCAAC
CAAGGGGACGAAGCCTGCACCCACTGTCCCATCAACAGCCGCACCACCTCCGAGGGTGCC
ACCAACTGTGTATGCCGCAACGGCTACTACAGGGCCGACCTGGACCCCTTAGACATGCCT
TGCACAACCATCCCCTCTGCGCCCCAGGCTGTGATCTCCAGCGTCAACGAGACGTCCCTC
ATGCTAGAGTGGACCCCACCCCGAGACTCGGGGGGTCGCGAGGATCTTGTTTACAACATC
ATCTGCAAGAGCTGTGGCTCCGGCCGGGGCGCATGCACGCGCTGCGGGGACAACGTGCAG
TACGCGCCCCGCCAGCTGGGCCTGACTGAGCCGCGCATCTACATCAGTGACCTGCTGGCA
CACACGCAGTACACCTTCGAGATCCAGGCCGTGAACGGTGTGACTGACCAGAGTCCCTTC
TCACCTCAGTTCGCCTCTGTGAACATCACCACCAACCAAGCAGCACCATCGGCCGTGTCC
ATCATGCACCAGGTGAGCCGCACTGTGGACAGCATCACCCTGTCGTGGTCCCAGCCAGAC
CAGCCCAACGGTGTGATCCTGGACTACGAGCTGCAGTACTATGAGAAGGAGCTCAGTGAG
TACAACGCCACGGCCATAAAAAGCCCCACCAACACAGTCACTGTGCAGGGCCTCAAAGCC
GGCGCCATCTATGTCTTCCAGGTGCGGGCACGCACCGTTGCAGGCTATGGGCGCTACAGT
GGCAAGATGTACTTCCAAACCATGACAGAAGCCGAGTACCAGACCAGCATCAAGGAAAAG
CTACCCCTCATCGTTGGCTCCTCCGCCGCCGGCTTAGTCTTCCTCATCGCTGTGGTCGTC
ATTGCCATCGTATGTAACAGACGGGGGTTTGAGCGTGCCGACTCAGAGTACACGGACAAG
CTACAACACTACACCAGCGGACACATGACCCCAGGCATGAAGATCTATATAGACCCTTTC
ACCTATGAAGATCCTAATGAGGCAGTGCGGGAGTTTGCCAAGGAAATTGACATCTCCTGT
GTCAAGATTGAGCAGGTGATCGGAGCAGGGGAATTTGGTGAGGTCTGCAGTGGCCATTTG
AAGCTGCCAGGCAAGAGAGAGATCTTTGTAGCCATCAAGACCCTCAAGTCAGGATACACG
GAGAAACAGCGCCGGGACTTCCTGAGTGAGGCATCCATCATGGGCCAGTTCGACCACCCC
AATGTCATCCATCTGGAAGGGGTTGTCACCAAGAGCACACCTGTCATGATCATCACTGAA
TTCATGGAGAACGGATCTCTGGACTCCTTCCTCCGGCAAAACGATGGGCAGTTCACAGTC
ATCCAACTGGTGGGCATGCTGAGGGGCATTGCAGCCGGCATGAAGTACCTGGCGGACATG
AACTACGTGCACCGTGACCTTGCTGCTCGAAACATCCTCGTCAACAGCAACCTGGTGTGT
AAGGTGTCTGATTTTGGGCTCTCACGCTTCCTGGAGGATGACACGTCTGACCCCACCTAT
ACCAGCGCTCTGGGTGGGAAGATCCCCATCCGTTGGACGGCACCGGAAGCCATCCAGTAC
CGGAAATTCACCTCGGCCAGTGATGTGTGGAGCTATGGCATCGTCATGTGGGAGGTGATG
TCCTACGGGGAACGACCCTACTGGGACATGACCAATCAAGACGTAATCAACGCCATTGAA
CAGGACTACAGACTACCTCCGCCCATGGACTGCCCTAGTGCCCTGCACCAGCTCATGCTG
GACTGCTGGCAGAAGGACCGCAACCACCGGCCCAAGTTCGGCCAGATTGTCAACACGCTG
GACAAGATGATCCGAAACCCCAACAGCCTCAAAGCCATGGCACCCCTGTCCTCTGGCATC
AACCTGCCACTGCTGGACCGCACGATACCGGACTACACCAGCTTTAACACGGTGGATGAG
TGGCTAGAGGCCATCAAGATGGGCCAGTACAAGGAGAGCTTTGCCAACGCCGGCTTTACC
TCTTTCGACGTTGTATCTCAGATGATGATGGAGGACATTCTCCGCGTTGGGGTCACTCTA
GCTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGTGATGCGGGCCCAGATGAACCAG
ATCCAGTCTGTAGAGGTT
Protein sequence predicted based on nucleotide sequence
MAVRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQ VCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADF DLATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRNGFYLAFQD YGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLY CNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGA TNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNI ICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPF SPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSE YNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIKEK LPLIVGSSAAGLVFLIAVVVIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPF TYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGHLKLPGKREIFVAIKTLKSGYT EKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTV IQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTY TSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIE QDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGI NLPLLDRTIPDYTSFNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVTL AGHQKKILNSIQVMRAQMNQIQSVEV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
106
End on reference sequence
3066
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
