Gene:
Gene Symbol:
Zfp628
Gene Name:
zinc finger protein 628
Original Clone ID:
FLH265299.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080678
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3093nts
Open reading frame: 1 to 3093
ATGGCGCCAGCTTCCACCACCGAGGGAACTGGGGAAAAGCCAGGGCCCACTGCGCCAGCA
CCCACCCCAGCTGCTCAGTATGAGTGTGGGGAATGCGGCAAGTCCTTCAGGTGGTCATCT
CGGCTCCTGCACCATCAGCGCACTCACACAGGTGAACGGCCCTATAAATGCCCGGACTGC
CCCAAAGCCTTCAAGGGTTCCTCAGCCCTCCTCTACCACCAGCGGGGCCATACAGGCGAG
CGGCCCTATCAGTGCCCAGACTGCCCCAAAGCCTTCAAGCGCTCTTCCTTGCTGCAGATC
CACCGCAGTGTGCACACAGGCCTGCGGGCCTTCACCTGCGGCCAGTGTGGTCTGGCCTTC
AAGTGGTCATCCCATTACCAATACCACTTGAGACAGCATACCGGTGAGCGGCCCTACCCG
TGCCCTGACTGCCCCAAGGCCTTCAAGAACTCCTCCAGCTTGCGGCGCCACCGGCATGTG
CACACAGGAGAACGCCCTTACACCTGTGGCATTTGTGGGAAGAGCTTTACTCAGAGCACC
AATCTGCGGCAGCACCAGCGGGTGCACACGGGCGAGCGCCCCTTCCGTTGCCCACTCTGC
CCCAAGACCTTCACACACTCCTCCAACCTGCTGCTGCACCACCGCACCCACGGCCCTGCC
CCCGGCCCCGCCCCTGCCCCCGCCCCCCCAGGGGAGACCAGCAGAGCTGACACCAAGGTT
CTGGTCTCTGATGCCTACTTGCAGCCCCGTAGCCCGCCTGAGCCTCCAGCCCCACCACCC
CAGCCCCCACCCGTGGTGCCCGAGCTTTTTTTGGCTGCTGCTGAGACTACAGTGGAGCTA
GTGTACCGCTGCGATGGCTGCGAGCAGGGCTTCAGCAGCGAGGAGCTCCTCCTGGAGCAC
CAGCCATGCCCGGGGCCCCCTGTAGCCACCCAGTCCCAGGATGTGCCTGCCGAGCTGCCC
CAGGCTGACTCCGCCCTACCACAGCCCCCACCTGCCACTCCAGGCCCTCCTAACTTTGCT
TGTCTTCCTTGTGGGAAGTCCTTCCGGACGGTGGCTGGCCTGTCCCGTCACCAGCACAGC
CATGGTGCGGCCAGTGGACAGGCCTTTCGCTGTGGCAGCTGTGATGGTGCCTTCCCACAA
CTGGCCAGCCTCTTGGCTCACCAGCAGTGTCACGTGGAGGAGGCGGCAGCCGGACGCCCA
CCTCCCCAGGCTGAGGTTGCGGAGGTCACCTGTCCCCAGGAACCTGTAGCCCCAGCCACT
CCTGCCCCACCCCCTCCTCCTCCACCTGCCCCGGTGGTTTCTGCAGAGCGGCCCTACAAA
TGTGCCGAGTGTGGGAAGGCCTTCAAGGGTTCTTCCGGGCTGCGCTACCACCTTCGGGAC
CACACAGGTGAGCGGCCCTACCAGTGTGGGGAGTGCGGCAAAGCCTTCAAACGCTCCTCC
CTGCTGGCCATCCACCAGCGTGTGCACACAGGCCTGCGAGCCTTCACCTGCGGCCAGTGT
GGCCTCACCTTCAAGTGGTCGTCCCACTATCAGTACCATCTGCGACTTCACTCGGGTGAG
CGGCCCTATGCCTGCACTGAGTGTGGCAAAGCCTTCCGTAACACTTCGTGCCTGCGGCGC
CACCGGCATGTGCACACCGGTGAGCGGCCCCACTCATGTAGTGTGTGTGGCAAGAGCTTC
GCACAGACGTCCAACTTGCGGCAGCACCAGCGGGTGCACACGGGCGAGCGCCCCTTCCGT
TGCCCGCTCTGCCCCAAGACCTTCACACACTCCTCCAACCTGCTGCTGCACCAGCGCACT
CATTCTGCCGAGCGCCCTTTTGCCTGCCCCATTTGTGGCCGTGGCTTTGTTATGGCTGCC
TACCTGCAGCGGCATCTGAGGACGCACACCCCAGCCACCACAACTTCGGGCACCACCGGC
TCTGCTGTAGCATCGCAGCCCCCTGCCCCATTGGCTGCAGCTCCAACTCCACTGGCTGCT
CAAGATGTTCACGTACTCCCTAACCTACAGGCCACACTTTCACTTGAAGTAGCTGGGGGA
ACAGCCCAGCCTACGCCCCCAGGCCCGGCTGCTCCCAGTTCTCAAACATTTCTCCTGGTA
CAGACTGCCCAGGGCCTCCAGCTGATTCCAAGCAGTGTCCAATCCCCTACTCCTCCACCC
CCGCCTCCACCCCCTAAAGTCATTCTGCTGCCTCCTGCCAGTGCTGGAGGTCCGGGCAGT
GGTGCTGCAAGGCCAGGCCCTCGGTCTGTTGGGAAAGCTGGACAGGGGACAGGGGTGGTG
TGGTTTCCAGGCCCAGGTGGTCTGGGGCTGCAAGGAGGGGCCAATGCAGGGGCTAGCGGG
GGAGGGCAGAGCCTCATTGTTCTGCAGAATGTAGGGAGTGGGGAGACAGGACCACAGGAA
GTGAGTGGGGTCCAGCTTCAGCCAGCACAGGAAGTGGCCACGGTACAACTACAGCCTGCA
CAGGAAGTGACTACAGTCCAGCTACAACCAGCACAGGAGGTGACTACGGTCCAGCTCCAG
CCCCTGACAGGCCAAGTCTCCAATTCTAATGGAGGAGCTGGCACTACAGAAGCTCCAAAC
CTGCTGCTGGTTCAGAGTGGGGCAACTGAAGAGTTGCTGACCGGCCCTGGGCCCGGGGAA
GTGGGAGACAGTGAGGCCGGTGCTGGTGTGGTCCAGGACGTCCTGTTTGAGACACTGCAG
ACAGATGAGGGGCTGCAGAGTGTGCTGGTGCTGAGTGGGGCGGATGGTGAGCAGACTAGG
CTCTGCGTGCAGGAGGTGGAAACGCTTTCCCCCGGTCTGGCGGAGCCAGCGGCCACTGGC
CCATCAGGACAGAAACTCCTTATCATTCGTAGCGCCCCAGCTACTGATCTGCTAGAAAAT
AGCAGCGTTGCAGGAGGCACCACAACACTGCAACTCCTGGCCCCGTCAGCACCTGGCCCG
GTCTCTGCCCCTGTGGGGGTGCCCGTAGCTCCCCCGTCCCAGATGGTACAAGTGGTCCCC
GCAGTGGCTGGACCAGGGGTCATGGCTCCCCAGAACTTGCCCTCCATCCAGATTGTGCAG
ACTCTTCCTGCAGTCCAACTGGTACACACGTTT
Protein sequence predicted based on nucleotide sequence
MAPASTTEGTGEKPGPTAPAPTPAAQYECGECGKSFRWSSRLLHHQRTHTGERPYKCPDC PKAFKGSSALLYHQRGHTGERPYQCPDCPKAFKRSSLLQIHRSVHTGLRAFTCGQCGLAF KWSSHYQYHLRQHTGERPYPCPDCPKAFKNSSSLRRHRHVHTGERPYTCGICGKSFTQST NLRQHQRVHTGERPFRCPLCPKTFTHSSNLLLHHRTHGPAPGPAPAPAPPGETSRADTKV LVSDAYLQPRSPPEPPAPPPQPPPVVPELFLAAAETTVELVYRCDGCEQGFSSEELLLEH QPCPGPPVATQSQDVPAELPQADSALPQPPPATPGPPNFACLPCGKSFRTVAGLSRHQHS HGAASGQAFRCGSCDGAFPQLASLLAHQQCHVEEAAAGRPPPQAEVAEVTCPQEPVAPAT PAPPPPPPPAPVVSAERPYKCAECGKAFKGSSGLRYHLRDHTGERPYQCGECGKAFKRSS LLAIHQRVHTGLRAFTCGQCGLTFKWSSHYQYHLRLHSGERPYACTECGKAFRNTSCLRR HRHVHTGERPHSCSVCGKSFAQTSNLRQHQRVHTGERPFRCPLCPKTFTHSSNLLLHQRT HSAERPFACPICGRGFVMAAYLQRHLRTHTPATTTSGTTGSAVASQPPAPLAAAPTPLAA QDVHVLPNLQATLSLEVAGGTAQPTPPGPAAPSSQTFLLVQTAQGLQLIPSSVQSPTPPP PPPPPKVILLPPASAGGPGSGAARPGPRSVGKAGQGTGVVWFPGPGGLGLQGGANAGASG GGQSLIVLQNVGSGETGPQEVSGVQLQPAQEVATVQLQPAQEVTTVQLQPAQEVTTVQLQ PLTGQVSNSNGGAGTTEAPNLLLVQSGATEELLTGPGPGEVGDSEAGAGVVQDVLFETLQ TDEGLQSVLVLSGADGEQTRLCVQEVETLSPGLAEPAATGPSGQKLLIIRSAPATDLLEN SSVAGGTTTLQLLAPSAPGPVSAPVGVPVAPPSQMVQVVPAVAGPGVMAPQNLPSIQIVQ TLPAVQLVHTF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
27
End on reference sequence
3122
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
