Gene:
Gene Symbol:
Sos1
Gene Name:
son of sevenless homolog 1 (Drosophila)
Original Clone ID:
FLH265305.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080684
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3957nts
Open reading frame: 1 to 3957
ATGCAGGCGCAGCAGCTGCCTTACGAGTTTTTCAGCGAGGAGAACGCGCCCAAGTGGCGG
GGGCTGCTGGTGCCTGCGCTGAAAAAGGTTCAGGGGCAAGTTCACCCTACTCTTGAGTCT
AATGATGATGCTCTTCAGTATGTTGAAGAATTAATTTTGCAATTACTAAATATGCTATGC
CAAGCTCAGCCCCGGAGTGCTTCAGATGTGGAGGAACGTGTTCAAAAGAGTTTTCCTCAT
CCAATTGATAAGTGGGCAATAGCTGATGCCCAATCAGCCATTGAAAAGAGGAAGAGACGA
AATCCTTTATCGCTGCCAGCAGAAAGAATTCATCATTTATTAAGGGAGGTCCTCGGTTAT
AAAATTGACCACCAGGTTTCTGTTTACATAGTAGCAGTATTAGAATACATTTCTGCAGAT
ATTTTAAAGCTCGTGGGGAATTATGTAAGAAATATACGGCATTATGAAATTACAAAACAA
GACATTAAAGTGGCAATGTGTGCTGATAAGGTATTGATGGATATGTTTCATCAAGATGTA
GAAGATATAAATATCTTATCTTTAACTGATGAAGAGCCTTCCACCTCAGGAGAACAAACT
TATTATGATTTGGTAAAAGCATTCATGGCAGAAATTCGACAGTATATAAGAGAATTAAAT
CTAATTATAAAAGTTTTTCGAGAGCCCTTTGTCTCTAATTCCAAATTGTTTTCATCTAAT
GATGTAGAAAACATATTCAGTCGTATAGTAGATATACATGAACTTAGTGTAAAGTTACTG
GGCCATATAGAAGATACTGTAGAAATGACAGATGAAGGCAGTCCCCACCCATTAGTAGGA
AGCTGTTTTGAAGACTTAGCAGAAGAACTGGCATTTGACCCGTATGAGTCATATGCTCGG
GATATTTTACGACCCGGATTCCATGGCCATTTTCTTAGTCAGTTATCAAAGCCTGGGGCA
GCACTTTATTTGCAGTCCATAGGCGAAGGCTTCAAAGAAGCTGTCCAGTACGTCCTGCCC
CGGCTGCTGCTTGCCCCTGTGTACCACTGTCTGCATTACTTTGAACTTCTGAAGCAGTTA
GAAGAAAAGAGTGAAGATCAAGAAGACAAGGAGTGTATGAAGCAAGCAATAACAGCCCTG
CTTAATGTCCAAAGTGGCATGGAAAAAATTTGCTCCAAAAGTCTTGCAAAACGAAGACTA
AGTGAGTCTGCATGTCGGTTTTACAGCCAGCAGATGAAGGGGAAACAGCTAGCCATCAAG
AAGATGAACGAGATCCAGAAGAACATTGATGGCTGGGAGGGGAAGGACATTGGACAGTGT
TGCAATGAGTTCATAATGGAAGGAACTCTTACACGTGTAGGAGCCAAACACGAGAGACAC
ATATTTCTCTTCGATGGCTTAATGATTTGCTGTAAATCAAACCATGGGCAGCCAAGACTC
CCTGGTGCTAGCAGTGCAGAATACCGGCTTAAAGAAAAGTTTTTTATGCGAAAGGTACAG
ATTAATGATAAAGATGACACCAGTGAGTACAAGCATGCTTTTGAAATCATTCTGAAAGAT
GGCAATAGTGTTATATTTTCTGCCAAGTCAGCTGAAGAGAAAAACAACTGGATGGCAGCA
CTGATCTCTTTGCAGTACCGCAGCACCCTGGAGAGGATGCTGGACGTAACGGTGCTGCAG
GAGGAGAAGGAGGAGCAGATGAGGCTGCCCAGTGCTGAAGTGTACAGGTTTGCAGAACCT
GACTCCGAGGAGAATATTCTATTCGAAGAGAATGTGCAGCCCAAAGCTGGGATCCCCATT
ATCAAGGCAGGGACAGTGCTTAAGCTCATTGAGAGGCTTACCTACCACATGTACGCAGAT
CCAAATTTTGTTCGGACGTTTCTTACAACATACAGGTCCTTTTGCAGACCTCAAGAACTA
CTGAGTCTTCTGATAGAAAGATTTGAAATTCCAGAGCCTGAGCCAACAGAAGCTGATCGC
ATAGCTATAGAGAATGGAGATCAGCCCCTGAGTGCAGAGCTGAAGAGGTTTAGAAAGGAA
TATATTCAGCCTGTGCAGTTGAGGGTGTTAAATGTGTGTCGGCACTGGGTGGAGCACCAT
TTCTATGACTTTGAAAGAGATGCAGACCTTTTACAGAGAATGGAGGAATTTATTGGAACA
GTAAGAGGTAAAGCAATGAAAAAATGGGTCGAATCCATCACTAAGATAATCCAAAGGAAA
AAAATTGCAAGAGACAATGGCCCAGGTCATAACATTACATTTCAGAGCTCACCTCCCACA
GTTGAGTGGCACATAAGCAGACCTGGGCACATAGAGACTTTTGACTTGCTCACCTTACAC
CCAATAGAAATTGCTCGGCAACTCACTTTACTTGAATCAGATCTATACCGGGCTGTGCAG
CCATCAGAATTAGTTGGAAGTGTGTGGACAAAAGAAGATAAAGAAATTAATTCTCCCAAC
CTTCTGAAGATGATTCGGCACACCACTAACCTCACTTTGTGGTTTGAGAAATGTATTGTA
GAAACAGAAAACTTAGAAGAAAGAGTAGCTGTAGTAAGTCGGATAATTGAGATTCTACAA
GTCTTTCAAGAGCTGAACAACTTCAATGGTGTCCTGGAAGTTGTCAGTGCTATGAACTCG
TCACCTGTTTACAGACTAGACCACACATTTGAGCAAATACCAAGCAGACAAAAGAAAATT
TTAGAAGAAGCTCATGAATTGAGTGAAGATCACTATAAGAAATATTTGGCAAAACTCAGG
TCTATTAATCCACCGTGTGTGCCTTTCTTTGGAATTTATCTCACAAATATCCTGAAGACA
GAAGAGGGCAACCCTGAGGTCCTGAGGAGACACGGGAAAGAGCTTATTAACTTCAGCAAG
AGGAGGAGAGTGGCCGAGATCACAGGCGAGATCCAGCAGTACCAGAACCAGCCCTACTGC
TTACGGGTGGAGCCGGACATCAAGAGGTTCTTTGAAAACTTGAATCCAATGGGAAACAGC
ATGGAGAAAGAATTTACAGACTATCTGTTCAACAAATCCCTAGAAATAGAACCCCGGCAC
CCTAAGCCTCTTCCGAGATTCCCAAAAAAATACAGCTATCCCCTAAAATCTCCTGGTGTT
CGTCCATCAAATCCAAGACCAGGAACCATGAGACATCCCACACCTCTGCAGCAGGAGCCA
AGAAAAATTAGCTACAGTCGGATTCCTGAAAGTGAGACGGAAAGCACAGCATCTGCACCA
AACTCCCCTCGGACCCCACTGACGCCGCCCCCTGCATCTGGCACCTCCAGCAACACAGAT
GTTTGCAGCGTGTTCGATTCTGACCACTCGGCAAGCCCTTTTCATTCAAGATCTGCTTCA
GTCTCATCTATAAGTTTATCCAAGGGCACTGATGAAGTGCCTGTCCCCCCTCCTGTACCC
CCTCGAAGACGTCCAGAGTCTGCCCCAGCTGAATCCTCCCCATCCAAGATTATGTCTAAG
CACTTGGACAGCCCCCCAGCTATTCCTCCTAGGCAACCCACATCCAAAGCCTATTCACCA
CGCTATTCAATATCAGATCGGACCTCTATATCAGATCCTCCTGAAAGCCCTCCCTTGTTA
CCACCACGGGAACCTGTGAGGACACCTGATGTTTTCTCAAGCTCACCATTACATCTCCAA
CCTCCTCCTTTGGGCAAAAAGAGTGATCATGGCAACGCCTTCTTCCCAAACAGCCCATCC
CCTTTTACACCGCCACCCCCCCAAACCCCCTCTCCTCATGGCACGAGAAGGCATCTGCCA
TCACCACCACTGACACAGGAGATGGACCTCCATTCCATTGCTGGGCCTCCTGTTCCTCCA
CGACAAAGCACTTCTCAACTTATCCCCAAACTCCCTCCAAAAACTTACAAAAGGGAGCAC
ACACACCCATCCATGCATAGAGATGGACCACCACTGCTGGAGAATGCCCATTCTTCC
Protein sequence predicted based on nucleotide sequence
MQAQQLPYEFFSEENAPKWRGLLVPALKKVQGQVHPTLESNDDALQYVEELILQLLNMLC QAQPRSASDVEERVQKSFPHPIDKWAIADAQSAIEKRKRRNPLSLPAERIHHLLREVLGY KIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDV EDINILSLTDEEPSTSGEQTYYDLVKAFMAEIRQYIRELNLIIKVFREPFVSNSKLFSSN DVENIFSRIVDIHELSVKLLGHIEDTVEMTDEGSPHPLVGSCFEDLAEELAFDPYESYAR DILRPGFHGHFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHCLHYFELLKQL EEKSEDQEDKECMKQAITALLNVQSGMEKICSKSLAKRRLSESACRFYSQQMKGKQLAIK KMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRL PGASSAEYRLKEKFFMRKVQINDKDDTSEYKHAFEIILKDGNSVIFSAKSAEEKNNWMAA LISLQYRSTLERMLDVTVLQEEKEEQMRLPSAEVYRFAEPDSEENILFEENVQPKAGIPI IKAGTVLKLIERLTYHMYADPNFVRTFLTTYRSFCRPQELLSLLIERFEIPEPEPTEADR IAIENGDQPLSAELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDADLLQRMEEFIGT VRGKAMKKWVESITKIIQRKKIARDNGPGHNITFQSSPPTVEWHISRPGHIETFDLLTLH PIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIV ETENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKI LEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLRRHGKELINFSK RRRVAEITGEIQQYQNQPYCLRVEPDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRH PKPLPRFPKKYSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESTASAP NSPRTPLTPPPASGTSSNTDVCSVFDSDHSASPFHSRSASVSSISLSKGTDEVPVPPPVP PRRRPESAPAESSPSKIMSKHLDSPPAIPPRQPTSKAYSPRYSISDRTSISDPPESPPLL PPREPVRTPDVFSSSPLHLQPPPLGKKSDHGNAFFPNSPSPFTPPPPQTPSPHGTRRHLP SPPLTQEMDLHSIAGPPVPPRQSTSQLIPKLPPKTYKREHTHPSMHRDGPPLLENAHSS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4490
Start on reference sequence
531
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
