DNASU Plasmid | Rufy1 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Rufy1

Gene Name: RUN and FYVE domain containing 1

Original Clone ID:

FLH265346.01F

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00080724

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2136nts

Open reading frame: 1 to 2136

ATGGCCGCCCGCGGAGAATGCCGCCGGGCGGGGCAGGATTCGGAGCCGGAGCCGGAGCGG
GAGCCGGAGTCCGAGCCGGGGCCGGAGCCTGAGCCCCAAGCAGGCCTAGAGTCCGGGGAA
GCGTTCGAGATCGTGGACCGGAGCCAGCTGCCGAGTCCCGGCGAGCTGCGGAGTGCGTCG
CGGCCTCGAGTGGCCGACAGCTGGTCGGCGCCCATCCTGACGCTGGCGCGCAGAGCCACC
GGGAACCTGTCGGCGAGCTGCGGCAGCGCGCTGCGCGCGGCCGCGGGGCTGGGAGACGGC
GGCGGCGGCGGGGAGCGCGCAGCTTCCAAAGGCCAGATGATGGAGGAGCGTGCCAACCTA
ATGCACATGATGAAACTCAGCATTAAGGTCTTACTCCAGTCTGCCCTTAGCCTGGGACGC
AGTCTGGATGCAGACTATGCCCCCTTGCAGCAGTTCTTTGTAGTGATGGAGCATTGCCTC
AAACATGGGCTGAAAGTCAAGAAGAGTTTCATTGGCCAAAATAAGTCTTTCTTTGGTCCT
TTGGAGCTGGTGGAAAAACTTTGTCCTGAAGCATCAGATATAGCTACCAGTGTCAGGAAT
CTTCCAGAACTAAAGACAGCCGTGGGAAGAGGCAGAGCGTGGCTTTATCTTGCTCTCATG
CAAAAGAAACTGGCAGATTATCTGAAAGTCCTCATAGACAACAAGCAGCTCCTAAGTGAG
TTCTATGAGCCCGAGGCCTTAATGATGGAGGAGGAAGGAATGGTGATCGTGGGTCTGCTG
GTGGGACTCAACGTTCTGGATGCCAACCTCTGCTTAAAGGGAGAAGACTTGGATTCTCAG
GTCGGGGTGATTGACTTCTCTCTCTGTCTGAAGGACGCACAGGATCTTGACAGCGGCAGA
GAGCATGAAAGAATTACCGATGTCCTTGATCAGAAAAATTACGTGGAGGAACTTAACCGG
CACCTAAGCTGCACAGTTGGGGATCTTCAGACGAAGATAGATGGCTTGGAAAAGACGAAC
TCAAAGCTTCAAGAAGAGCTTTCAGCTGCAACAGACCGAATTTGTTCCCTACAAAAAGAA
CAACAGCAGCTGAGAGAACAGAACGAAGTAATTCGAGAAAGAAGCGAGAAGAGCGTGGAG
ATAACAAAACAGGACACCAAGGTTGAACTGGAGACTTATAAGCAGACCCGGCAGGGCCTG
GATGAAATGTACAGCGATGTGTGGAAACAGCTGAAGGAGGAGAAGAAAGTCCGACTGGAA
CTGGAAAAGGAGCTGGAGTTGCAGATTGGAATGAAGACGGAGATGGAGATCGCCATGAAG
TTGCTGGAGAAGGACACCCATGAGAAGCAAGACACGCTCGTTGCCCTCCGCCAGCAGCTG
GAGGAAGTCAAAGCTATTAACTTACAGATGTTTCACAAAGTTCAGAGTGCAGAGAGCAGT
TTACAGCAGAAGAATGAAGCCATCGCATCTTTTGAAGGAAAAACCACTCAGGTGATGTCC
AGCATGAAGCAAATGGAGGAAAGGTTACAGCAGGCGGAACGGGCCAGGCAGGCGGCAGAA
GAGCGAAGCCACAAGTTGCAGCAGGAGCTCAGCGGGAGGGGCAGTGCCCTGCAGCTCCAG
CTGTCCCAGCTGCGGGACCAATGCTCAGGTCTAGAGAAAGAACTGAAGTCAGAAAAAGAA
CAAAGACAAGCTCTCCAGAGAGAACTACAGCGTGAGAAAGACACCTCTTGTCTGCTCCAG
ACGGAGCTGCAGCAAGTGGAAGGCCTGAAGAAGGAGCTGCGGGAGCTCCAAGACGAGAAG
GCAGAGTTACGGAAGGTCTGTGAGGAGCAGGAGCAAGCCCTCCAGGAAATGGGACTACAC
CTTAGCCAGTCCAAACTGAAGATGGAAGACATAAAGGAAGTAAATAAGGCACTGAAGGGC
CACACATGGTTGAAAGATGACGAGGCAACACATTGTAAGCAGTGTGAGAAGGACTTCTCC
ATTTCTCGGAGGAAGCACCACTGCCGAAACTGTGGCCACATTTTCTGCAACACCTGCTCT
AGCAACGAGCTGGCCCTGCCCTCCTACCCTAAGCCAGTGCGTGTGTGTGACAGCTGCCAT
ACCCTGCTACTGCAGCGCTGCTCCTCCACTGCCTCC

Protein sequence predicted based on nucleotide sequence

MAARGECRRAGQDSEPEPEREPESEPGPEPEPQAGLESGEAFEIVDRSQLPSPGELRSAS
RPRVADSWSAPILTLARRATGNLSASCGSALRAAAGLGDGGGGGERAASKGQMMEERANL
MHMMKLSIKVLLQSALSLGRSLDADYAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGP
LELVEKLCPEASDIATSVRNLPELKTAVGRGRAWLYLALMQKKLADYLKVLIDNKQLLSE
FYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVIDFSLCLKDAQDLDSGR
EHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAATDRICSLQKE
QQQLREQNEVIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLE
LEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKVQSAESS
LQQKNEAIASFEGKTTQVMSSMKQMEERLQQAERARQAAEERSHKLQQELSGRGSALQLQ
LSQLRDQCSGLEKELKSEKEQRQALQRELQREKDTSCLLQTELQQVEGLKKELRELQDEK
AELRKVCEEQEQALQEMGLHLSQSKLKMEDIKEVNKALKGHTWLKDDEATHCKQCEKDFS
ISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2146

Start on reference sequence 8

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Rufy1

Symbol Nomenclature :

Rufy1

SYNONYM :

3000002E04Rik; Rabip4; ZFYVE12

Designation :

FYVE-finger containing protein|RUN and FYVE domain-containing protein 1|Rab4a interacting protein|rab4-interacting protein

Full Nomenclature :

RUN and FYVE domain containing 1

GENEID :

216724

Locus Tag :

RP23-121P11.2

GI :

151556605

GenBank Accession :

BC148344

Mouse Genome Informatics :

2429762

Vega :

OTTMUSG00000005584

Target GenBank:

NM_172557

Protein

SMART domain :

FYVE : Protein present in Fab1, YOTB, Vac1, and EEA1

SMART domain :

RUN :

UniProt :

Q8BIJ7

Clone

Cloning Information :

265346

HIP Master Clone ID :

197444

IMAGE ID :

100015279

Original Clone ID :

FLH265346.01F

Organism

Species Specific ID:

216724

Genome

Chromosome :

Map Location :

11 B1.3|11

Ensembl :

ENSMUSG00000020375

Reagents

Labome :

Rabip4-antibody