Gene:
Gene Symbol:
Clcn6
Gene Name:
chloride channel 6
Original Clone ID:
FLH265347.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080725
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2610nts
Open reading frame: 1 to 2610
ATGGCGGGGTGCAGGGGGTCCGTGTGCTGCTGCTGCCGGTGGTGTTGCTGCTGCGGTGAA
CGGGAGAGCCGCACCCCCGAGGAGCTGACCATCCTTGGGGAGACACAGGAAGAAGAGGAT
GAGATTCTTCCAAGGAAAGACTATGAGAGTCTGGACTATGACCGCTGCATCAATGACCCT
TACCTGGAGGTGTTGGAGACCATGGATAATAAGAAAGGGCGAAGATACGAGGCGGTGAAG
TGGATGGTGGTGTTCGCCATTGGCGTCTGTACGGGGCTGGTGGGTCTCTTTGTGGACTTT
TCCGTGCGACTCTTCACCCAACTGAAGTTCGGAGTGGTTCAAACGTCGGTGGAGGAATGC
AGTCAGAAGGGCTGCCTTGCCCTGTCCCTCCTGGAGCTCCTGGGCTTTAACTTGACCTTT
GTCTTCCTGGCAAGCCTTCTTGTCTTGATAGAGCCAGTGGCAGCAGGTTCTGGAATCCCC
GAGATCAAGTGCTACCTGAATGGTGTGAAAGTGCCAGGAATTGTCCGGCTCCGGACCCTG
CTCTGCAAAGTCTTTGGGGTGCTGTTCAGTGTGTCTGGAGGGCTCTTTGTGGGGAAAGAA
GGCCCCATGATCCACAGTGGTGCTGTGGTAGGAGCTGGCCTCCCTCAGTTCCAGAGCATC
TCCTTACGGAAGATCCAGTTTAACTTCCCCTACTTCCGCAGCGACAGAGACAAGCGTGAC
TTTGTGTCAGCGGGGGCAGCTGCTGGCGTTGCTGCAGCTTTTGGGGCCCCTATAGGGGGC
ACCTTGTTCAGTCTGGAGGAGGGTTCATCCTTCTGGAACCAGGGGCTCACGTGGAAAGTG
CTCTTCTGTTCCATGTCCGCCACCTTCACCCTCAACTTCTTCCGCTCTGGGATTCAGTTT
GGAAGTTGGGGTTCCTTCCAGCTGCCTGGATTGCTGAACTTTGGTGAATTTAAGTGCTCT
GACTCTGATAAAAAATGTCATCTCTGGACAGCTATGGATTTGGGTTTCTTCGTCGTGATG
GGGGTCATTGGGGGCCTCCTGGGAGCCACATTCAACTGTCTGAACAAGAGGCTTGCAAAG
TACCGTATGCGCAACGTGCACCCGAAACCTAAGCTCGTCAGAGTCTTAGAGAGCCTCTTG
GTGTCCCTGGTGACCACTGTGGTGGTGTTTGTGGCCTCCATGGTGTTAGGAGAATGCCGA
CAGATGTCTTCCACGAGTCAAACCGGTAATGGCTCATTCCAGCTCCAGGTCACATCAGAA
GATGTGAATTCAACTATCAAGGCTTTTTTCTGTCCCAATGACACTTACAATGACATGGCC
ACACTCTTCTTCAACTCCCAGGAGTCTGCCATCCTGCAGCTCTTCCATCAGGATGGGACT
TTCAGCCCCGTCACCCTGGCCTTATTCTTCATCCTCTATTTCCTGCTCGCATGCTGGACT
TTTGGCACATCTGTCCCCAGTGGCCTCTTCGTGCCTTCCCTGCTGTGTGGAGCAGCTTTT
GGACGTTTAGTTGCCAATGTCCTGAAAAGCTACATTGGACTGGGCCACCTCTATTCTGGG
ACCTTTGCCCTGATTGGTGCGGCAGCTTTCTTGGGTGGGGTTGTAAGGATGACCATCAGC
CTCACAGTCATCCTGATCGAGTCTACAAATGAGATCACCTATGGGCTTCCGATCATGGTC
ACTCTGATGGTGGCCAAGTGGACTGGAGACTTGTTCAACAAGGGCATTTATGACGTCCAT
ATTGGGCTGAGAGGTGTGCCTCTTCTGGAGTGGGAGACAGACGTGGAAATGGACAAGCTG
AGAGCCAGTGACATCATGGAGCCCAACCTGACGTACGTCTACCCGCACACTCGCATCCAG
TCTCTGGTGAGCATCCTGCGCACTACGGTCCACCATGCCTTCCCAGTGGTCACGGAGAAC
CGGGGCAACGAGAAGGAGTTCATGAAGGGCAACCAGCTCATAAGCAACAACATCAAGTTC
AAGAAATCCAGTATCCTGACCCGTGCTGGTGAGCAGCGCAAGCGGGGCCAGTCCATGAAG
TCGTACCCTTCCAGCGAGCTGCGCAACGTGTGTGATGAGCATGTGGCTTCTGAGGAGCCG
GCAGAGAAGGAGGACCTGCTACAGCAGATGCTGGAGAGGAGATACACTCCCTACCCCAAC
CTATACCCTGACCAGTCCCCGAGTGAAGACTGGACCATGGAGGAGCGTTTCCGTCCGCTG
ACCTTTCACGGCCTTGTCCTGCGCTCTCAGCTTGTCACCCTGCTTGTCCGAGGAGTGTGC
TATTCGGAAAGTCAGTCCAGTGCCAGCCAACCACGTCTTTCTTACGCTGAGATGGCTGAG
GACTACCCGCGATACCCGGACATACATGACCTGGACCTCACACTGCTGAATCCCCGAATG
ATTGTGGATGTCACCCCATATATGAATCCTTCACCATTTACCGTCTCCCCCAACACCCAT
GTTTCTCAAGTGTTCAACCTGTTCAGAACCATGGGCCTGCGCCACTTGCCTGTGGTGAAT
GCAGTAGGAGAGATCGTGGGGATCATTACACGGCACAACCTGACAAATGAATTCCTGCAG
GCCCGGCTGCGGCAGCATTACCAGACCCTC
Protein sequence predicted based on nucleotide sequence
MAGCRGSVCCCCRWCCCCGERESRTPEELTILGETQEEEDEILPRKDYESLDYDRCINDP YLEVLETMDNKKGRRYEAVKWMVVFAIGVCTGLVGLFVDFSVRLFTQLKFGVVQTSVEEC SQKGCLALSLLELLGFNLTFVFLASLLVLIEPVAAGSGIPEIKCYLNGVKVPGIVRLRTL LCKVFGVLFSVSGGLFVGKEGPMIHSGAVVGAGLPQFQSISLRKIQFNFPYFRSDRDKRD FVSAGAAAGVAAAFGAPIGGTLFSLEEGSSFWNQGLTWKVLFCSMSATFTLNFFRSGIQF GSWGSFQLPGLLNFGEFKCSDSDKKCHLWTAMDLGFFVVMGVIGGLLGATFNCLNKRLAK YRMRNVHPKPKLVRVLESLLVSLVTTVVVFVASMVLGECRQMSSTSQTGNGSFQLQVTSE DVNSTIKAFFCPNDTYNDMATLFFNSQESAILQLFHQDGTFSPVTLALFFILYFLLACWT FGTSVPSGLFVPSLLCGAAFGRLVANVLKSYIGLGHLYSGTFALIGAAAFLGGVVRMTIS LTVILIESTNEITYGLPIMVTLMVAKWTGDLFNKGIYDVHIGLRGVPLLEWETDVEMDKL RASDIMEPNLTYVYPHTRIQSLVSILRTTVHHAFPVVTENRGNEKEFMKGNQLISNNIKF KKSSILTRAGEQRKRGQSMKSYPSSELRNVCDEHVASEEPAEKEDLLQQMLERRYTPYPN LYPDQSPSEDWTMEERFRPLTFHGLVLRSQLVTLLVRGVCYSESQSSASQPRLSYAEMAE DYPRYPDIHDLDLTLLNPRMIVDVTPYMNPSPFTVSPNTHVSQVFNLFRTMGLRHLPVVN AVGEIVGIITRHNLTNEFLQARLRQHYQTL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2613
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
