Gene:
Gene Symbol:
Col11a2
Gene Name:
collagen, type XI, alpha 2
Original Clone ID:
FLH265352.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080730
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4950nts
Open reading frame: 1 to 4950
ATGGAGCGGTGCAGCCGCTGCCACCGCCTCCTTCTGTTCCTACCTCTGGTGCTGGGTCTG
AGCGCTGCCCCGGGATGGGCAGGTGCTCCCTCTGTGGATGTGCTTCGTGCCCTGAGGTTC
CCCTCCCTTCCCGATGGTGTTCGGAGATCAAAAGGGGTCTGTCCGGGTGATGTGGCTTAC
CGTGTGGCACGGCCTGCCCAGCTCAGCGCACCCACGCGCCAGCTCTTCCCAGGAGGCTTT
CCCAAAGACTTCTCTCTGCTGACGGTTGTCCGGACCCGCCCTGGCCTCCAGGCTCCCCTC
TTGACTCTATACAGCGCCCAGGGAGTCCAGCAGCTGGGCTTGGAGCTCGGCCGCCCTGTC
CGCTTTCTCTATGAGGACCAGAGGGGACGGCCACAAGCCTCCGCTCAGCCCATCTTCCGA
GGCCTCAGCCTAGCAGATGGCAAATGGCACCACGTGGCTGTGGCTGTGAAGGGTCAGTCT
GTCACTCTCATTGTGGACTGTAAGAAGCGAGTTACCCGGCCCCTTCCCAGAAGTGTGCAT
CCGGTGTTGGACACCCACGGGGTGGTGATCTTTGGTGCCCACATCCTCGACGATGAAGTC
TTTGAAGGCGATGTTCAGGAGCTCCTCGTTGTCCCAGGCGTCCAAGCTGCCTATCAGTCT
TGTGGGCAGAAGGATCTGGAATGTGAGAGAGAACAGAGGGACGGCCCTCAGACTCAGAAG
CCTCACAGAGCCCAGAGATCTCCAAAGAAGGAACCAGCAAGACTTCATAAGCCACAGAGC
CAGGAGCCCCAGAAGCAGTACCCCACCCCAGGTGAAGAGGAAGGAGTCCTGGAGTCCAGT
CCCTTGCCATTCCTTGAAGAGGTTGCCCACGGACCCCGGGGGCTAAAGGGAGAGAAGGGA
GAGCCTGCAGTGCTGGAGCCTGGTATGTTTGTAGAGGGACCCCCAGGCCCAGAAGGCCCA
GCGGGATTAGCTGGACCCCCTGGCATCCAGGGGAACCCAGGCCCGGTTGGAGACCCCGGT
GAGAGGGGCCCCCCTGGCCGAGCAGGGCTCCCCGGATCAGATGGACCCCCTGGTCCTCCC
GGCACATCTCTGATGCTTCCATTCCGGTTTGGCAGTAGTGGGGGTGACAAGGGCCCCGTG
GTGGCAGCCCAGGAGGCCCAGGCCCAGGCGATTCTGCAGCAGGCACGGCTGGCACTCCGT
GGGCCCCCTGGCCCCATGGGTTACACGGGCCGCCCTGGACCATTGGGTCAGCCTGGGAGC
CCTGGCTTGAAGGGAGAATCTGGAGATCTGGGCCCACAGGGCCCCAGAGGACCTCAGGGC
CTCACAGGTCCTCCTGGCAAGGCTGGACGAAGGGGCCGAGCAGGTGCTGATGGAGCCCGT
GGGATGCCGGGAGAACCTGGCATGAAGGGTGACCGAGGTTTCGACGGACTTCCAGGGCTA
CCTGGCGAGAAGGGACAAAGGGGTGATACAGGTGCTCAGGGCCTTCCTGGGCCTCCTGGT
GAGGACGGAGAGAGGGGTGATGATGGAGAGATTGGGCCACGGGGGCTGCCTGGAGAGTCG
GGACCTAGAGGACTCCTTGGCCCTAAAGGCCCGCCTGGTATTCCTGGGCCGCCGGGAGTC
CGAGGCATGGACGGTCCCCACGGCCCCAAAGGGAGCTTGGGACCTCAAGGAGAGCCAGGA
CCTCCTGGACAACAGGGTACTCCTGGGGCCCAGGGCCTCCCCGGACCTCAGGGAGCCATC
GGTCCTCATGGAGAGAAGGGTGCTCGTGGGAAACCAGGCCTCCCTGGCATGCCTGGATCA
GATGGACTCCCGGGTCACCCAGGGAAGGAAGGTCCCCCTGGAACCAAAGGGAACCAGGGC
CCGTCCGGACCACAGGGTCCTCTAGGATACCCAGGCCCTCGAGGCGTCAAGGGTGTGGAT
GGAATTCGGGGCCTGAAGGGCCACAAGGGTGAAAAGGGCGAGGACGGCTTTCCTGGGTTC
AAAGGTGACATAGGAGTGAAAGGAGACAGGGGCGAGGTTGGAGTCCCTGGTTCCAGGGGC
GAAGACGGCCCTGAAGGGCCAAAAGGGCGCACTGGACCTACAGGAGACCCTGGACCCACT
GGGCTCATGGGCGAGAAGGGCAAGCTAGGTGTTCCTGGTCTGCCTGGCTATCCTGGACGC
CAGGGCCCCAAGGGATCTCTGGGTTTCCCTGGTTTTCCTGGAGCCAGTGGAGAGAAGGGA
GCTCGGGGCCTGTCTGGGAAATCAGGACCTCGGGGAGAACGGGGCCCCACGGGTCCAAGG
GGTCAGCGGGGACCTCGAGGTGCCACTGGGAAATCTGGAGCTAAGGGAACATCAGGTGGT
GACGGTCCCCACGGGCCACCCGGAGAGAGGGGTCTTCCTGGACCTCAAGGCCCCAATGGA
TTTCCTGGCCCCAAAGGCCCTCCGGGCCCTGCAGGGAAGGATGGGCTGCCGGGACACCCC
GGCCAGAGAGGAGAAGTGGGATTCCAAGGAAAGACCGGCCCACCAGGCCCGCCCGGAGTG
GTGGGACCTCAGGGAACAGCTGGAGAAAGTGGTCCCATGGGAGAGAGAGGTCACTCTGGC
CCCCCAGGACCTCCTGGAGAGCAAGGATTGCCTGGAACATCTGGGAAAGAAGGGACCAAG
GGTGACCCTGGTCCTCCTGGGGCCCCAGGGAAGGATGGTCCTGCTGGTCTGAGAGGCTTC
CCAGGAGAGCGAGGCCTTCCAGGCACTGCTGGTGGACCCGGCTTGAAAGGAAATGAAGGT
CCAGCTGGCCCTCCTGGCCCTGCAGGCTCTCCTGGCGAGCGAGGTGCAGCAGGATCAGGG
GGCCCCATTGGTCCCCCGGGACGTCCAGGCCCACAAGGTCCCCCTGGAGCAGCAGGAGAG
AAAGGCGTACCGGGCGAGAAAGGCCCTATTGGTCCCACTGGTCGTGATGGGGTGCAGGGC
CCCGTGGGGCTTCCTGGTCCTGCAGGACCCCCAGGCGTGGCTGGAGAGGATGGAGACAAG
GGTGAAGTGGGAGACCCTGGACAGAAGGGAACCAAAGGAAACAAGGGTGAACATGGCCCT
CCTGGACCTCCTGGTCCCATCGGGCCTGTGGGGCAACCTGGAGCTGCGGGAGCTGATGGT
GAGCCTGGAGCTCGGGGACCCCAGGGACACTTTGGAGCCAAAGGTGATGAAGGAACAAGA
GGGTTCAATGGACCCCCGGGACCCATCGGCCTACAGGGCCTGCCAGGACCCTCTGGGGAG
AAAGGAGAAACAGGAGACGGGGGGCCTATGGGACCCCCTGGCCCTCCAGGACCTCGAGGC
CCCGCTGGACCCAATGGTGCTGATGGCCCACAAGGTTCCCCTGGAGGTGTTGGAAACTTG
GGTCCCCCTGGAGAAAAGGGTGAACCGGGGGAGTCAGGGTCTCCAGGCGTCCAGGGCGAG
CCGGGCGTCAAGGGACCACGTGGAGAGCGTGGTGAGAAAGGAGAGTCTGGGCAGGCGGGA
GAGGCTGGACCACCGGGGCCCAAAGGCCCTACAGGCGACAATGGCCCCAAGGGGAACCCT
GGTCCTGTTGGCTTTCCTGGGGACCCTGGCCCCCCTGGAGAAGCTGGCCCACGGGGCCAG
GATGGTGCTAAGGGAGACCGAGGCGAGGATGGCGAGCCAGGACAACCTGGATCCCCTGGT
CCCACCGGGGAGAATGGGCCCCCTGGACCCCTTGGAAAGCGGGGACCTGCTGGCACTCCT
GGTCCAGAAGGACGGCAAGGAGAGAAGGGAGCTAAGGGGGACCCTGGTGCTGTGGGGGCC
CCGGGAAAGACAGGCCCTGTGGGTCCTGCAGGCCTAGCAGGAAAGCCCGGCCCCGATGGT
CTTCGGGGGCTCCCGGGTTCAGTGGGTCAGCAAGGCCGCCCTGGAGCCACAGGCCAGGCT
GGGCCCCCAGGTCCTGTGGGACCCCCAGGGCTTCCTGGCCTCCGGGGTGATGCTGGAGCC
AAGGGGGAAAAGGGTCACCCAGGTCTCATCGGACTGATTGGGCCGACTGGAGAGCAAGGC
GAGAAGGGCGACCGTGGCCTCCCTGGACCTCAGGGCTCACCCGGACAGAAGGGAGAGACG
GGTATCCCAGGAGCATCTGGCCCCATCGGTCCTGGAGGGCCTCCTGGCCTGCCTGGACCC
TCTGGCCCCAAAGGAGCCAAAGGAGCCACAGGCCCAGCTGGACCCAAGGGAGAGAAGGGT
GTCCAGGGCCCTCCAGGACACCCGGGCCCCCCGGGAGAGGTGATCCAGCCACTGCCCATC
CAGATGCCCAAGAAGACCCGCCGTTCCGTGGACGGAAGCAAACTGATACAGGATGAGGAG
GCTGTGCCCACTGGCGGTGCTCCGGGCAGTCCTGCGGGGCTGGAGGAGATCTTTGGCTCA
CTGGACTCTCTGCGGGAGGAGATCGAGCAGATGAGGAGGCCGGCGGGGACCCAGGACAGC
CCTGCTCGCACCTGCCAGGACTTGAAGCTGTGCCACCCGGAGCTTCCTGATGGAGAGTAC
TGGGTTGACCCTAACCAGGGCTGTGCTCGGGATGCCTTCCGGGTGTTCTGCAACTTCACA
GCAGGAGGGGAGACGTGTGTCACACCCAGGGATGACGTCACACAGTTCTCCTACGTGGAC
TCCGAGGGCTCCCCAGTGGGCGTGGTCCAGCTCACCTTCCTGCGGCTGCTCAGCGTCTCT
GCCCACCAGGATGTCTCCTACCCTTGCTCTGGAGTATCCCAGGATGGTCCCCTGAAACTC
CGAGGGGCCAACGAGGATGAGCTGAGCCCTGAGACCAGCCCTTATGTCAAGGAGTTCAGA
GATGGCTGTCAGACCCAGCAAGGCCGGACGGTGTTGGAGGTGCGCACGCCTGTACTGGAG
CAGCTGCCCGTGCTGGATGCCTCCTTCGCAGACCTGGGGGCCCCCACAAGACGGGGAGGG
GTGCTTCTGGGGCCTGTCTGCTTCATGGGC
Protein sequence predicted based on nucleotide sequence
MERCSRCHRLLLFLPLVLGLSAAPGWAGAPSVDVLRALRFPSLPDGVRRSKGVCPGDVAY RVARPAQLSAPTRQLFPGGFPKDFSLLTVVRTRPGLQAPLLTLYSAQGVQQLGLELGRPV RFLYEDQRGRPQASAQPIFRGLSLADGKWHHVAVAVKGQSVTLIVDCKKRVTRPLPRSVH PVLDTHGVVIFGAHILDDEVFEGDVQELLVVPGVQAAYQSCGQKDLECEREQRDGPQTQK PHRAQRSPKKEPARLHKPQSQEPQKQYPTPGEEEGVLESSPLPFLEEVAHGPRGLKGEKG EPAVLEPGMFVEGPPGPEGPAGLAGPPGIQGNPGPVGDPGERGPPGRAGLPGSDGPPGPP GTSLMLPFRFGSSGGDKGPVVAAQEAQAQAILQQARLALRGPPGPMGYTGRPGPLGQPGS PGLKGESGDLGPQGPRGPQGLTGPPGKAGRRGRAGADGARGMPGEPGMKGDRGFDGLPGL PGEKGQRGDTGAQGLPGPPGEDGERGDDGEIGPRGLPGESGPRGLLGPKGPPGIPGPPGV RGMDGPHGPKGSLGPQGEPGPPGQQGTPGAQGLPGPQGAIGPHGEKGARGKPGLPGMPGS DGLPGHPGKEGPPGTKGNQGPSGPQGPLGYPGPRGVKGVDGIRGLKGHKGEKGEDGFPGF KGDIGVKGDRGEVGVPGSRGEDGPEGPKGRTGPTGDPGPTGLMGEKGKLGVPGLPGYPGR QGPKGSLGFPGFPGASGEKGARGLSGKSGPRGERGPTGPRGQRGPRGATGKSGAKGTSGG DGPHGPPGERGLPGPQGPNGFPGPKGPPGPAGKDGLPGHPGQRGEVGFQGKTGPPGPPGV VGPQGTAGESGPMGERGHSGPPGPPGEQGLPGTSGKEGTKGDPGPPGAPGKDGPAGLRGF PGERGLPGTAGGPGLKGNEGPAGPPGPAGSPGERGAAGSGGPIGPPGRPGPQGPPGAAGE KGVPGEKGPIGPTGRDGVQGPVGLPGPAGPPGVAGEDGDKGEVGDPGQKGTKGNKGEHGP PGPPGPIGPVGQPGAAGADGEPGARGPQGHFGAKGDEGTRGFNGPPGPIGLQGLPGPSGE KGETGDGGPMGPPGPPGPRGPAGPNGADGPQGSPGGVGNLGPPGEKGEPGESGSPGVQGE PGVKGPRGERGEKGESGQAGEAGPPGPKGPTGDNGPKGNPGPVGFPGDPGPPGEAGPRGQ DGAKGDRGEDGEPGQPGSPGPTGENGPPGPLGKRGPAGTPGPEGRQGEKGAKGDPGAVGA PGKTGPVGPAGLAGKPGPDGLRGLPGSVGQQGRPGATGQAGPPGPVGPPGLPGLRGDAGA KGEKGHPGLIGLIGPTGEQGEKGDRGLPGPQGSPGQKGETGIPGASGPIGPGGPPGLPGP SGPKGAKGATGPAGPKGEKGVQGPPGHPGPPGEVIQPLPIQMPKKTRRSVDGSKLIQDEE AVPTGGAPGSPAGLEEIFGSLDSLREEIEQMRRPAGTQDSPARTCQDLKLCHPELPDGEY WVDPNQGCARDAFRVFCNFTAGGETCVTPRDDVTQFSYVDSEGSPVGVVQLTFLRLLSVS AHQDVSYPCSGVSQDGPLKLRGANEDELSPETSPYVKEFRDGCQTQQGRTVLEVRTPVLE QLPVLDASFADLGAPTRRGGVLLGPVCFMG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
219
End on reference sequence
5171
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
