DNASU Plasmid | Zfp352 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Zfp352

Gene Name: zinc finger protein 352

Original Clone ID:

FLH265356.01F

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00080734

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1647nts

Open reading frame: 1 to 1647

ATGGCAGAAAACACAGGTGCTTCCCAGCCAGGACCCTGCAATACACAGCCTTCCCCTGTG
GAGTCCAGCCAGCTTTTCTCAGGTCAGAGCTCAGAGATGCTTACCTGGAAACAAACAGTG
ATGGAATCTGCATCCTGTAGCACACAGGAGACAACCTGTACTCAGAACTCAACAGCATAC
CCAGGAAAAGCCATTGGTTTCCATTTTGGACAGCCATTTGAGAACACTTCTTGGAACCAG
CAAGTTGTGTCTGCTGAGCAGAAAATATCTCCTGCAGGTCTTTACCATACACAGCTCAAC
AGCGCCCCTCAGATATGCCTTCCTCCCCAGAAGACAACAAGAGTTGACACCAGGGAGACT
TTCTACAATGAAGACTACTGGATGAGGACACTGAACTCTGACAAGACTCTCTTGACCCAA
AATACAGCCATGTGTGAGAATCAGAGGGTTACCTTTGATAATCATCAAATTGCTCTCTAT
GGAAGCCATGCTGGCCAGGACCCAAATGTGGGTCACGCCTTTGCTTCTGAACATGACTGG
ACCTCCTACAACAGTCATATGACAACCAGGAATGGAGGGCATCTGACTCTCTATGAGAGT
CAGATGACAGCACCCAGTTGTAGCCAGACCCTTCATCCCAATCAAATCATAACATCCTTT
TCTGAACAGAATTATTTTGAGGATCAGCAGAGTAACCTAGCAGTTAATAATGGATTCTAT
GGAAATCAGGTGATATTGCCAAATGGATACCAGGCTTTCTATGAGCCTCAGATGAGAGCT
AACTTTGACCAAACAAATGACCAAATGAAATCCTTCAGTGGTCAGAATGTCTGCAAAGGT
CAGGAGAATCTCCTCAGTGGTGAGTGCTCCCTCTCTGGTTATCAGACATCAGGCTACAGA
TGTGACCAGGATTTGATTGTGAATCCTCAGGTGACAAGTCCAATTGGTGGCCAGCCTCTC
TCTGACTTTCAGACACAAACCTCAAGTTTTGATACAGCTCTTCCTGGGTCTAAGAGGACA
AGACCCAGTGCAGAAGACAACCTGGCTTGCCCTTTGGAGACAAGTCCCACATCTGAAGAA
ACACTCTACTTGGGTCAGATGAGGACCTCTGTTGATCAAAATTTCTACTCAAGTCAGAAT
GGAACACCTAATATTAAAGAAAGCCTTGATCCTCAGGTGACTTCATTTAGTTTGCAAGCT
CCATATGTGGGTGAATCCTCATACCCTTCATGTTCCCCTTTGATTCAAAAACAACCTCAG
AAAAACTCTTCGGCTTCCAGTTTAGTCCAGAGACAGCATCCAGAGATGAAGTTAGACTTC
AAGACCCAGAGCCCTGTGTCACAGAAGGACCCTAGTCCTTTGAAGCTCTACTTTTGTACT
TATAAGGGTTGTACGAAATTTTACAAAAGAGCTTACCACCTCAAAGAACACCAGAAGAAA
CACACTGATAAGAGGAAATACGGGTGTGATGAGCCTGGGTGCACATGGTCGTTTTTCCGC
CTATGTGATCTCAACAGACACAAAGAAAAGCACAATGGTGAGAGGTTCTACGCTTGTCCC
TTGTGCTCTACAAACTATTCCAGACTGCTTTATCTCAAAAAGCATCTGGAGAAGAAACAT
GCTCAAGCACAACCCACCACTGCAACC

Protein sequence predicted based on nucleotide sequence

MAENTGASQPGPCNTQPSPVESSQLFSGQSSEMLTWKQTVMESASCSTQETTCTQNSTAY
PGKAIGFHFGQPFENTSWNQQVVSAEQKISPAGLYHTQLNSAPQICLPPQKTTRVDTRET
FYNEDYWMRTLNSDKTLLTQNTAMCENQRVTFDNHQIALYGSHAGQDPNVGHAFASEHDW
TSYNSHMTTRNGGHLTLYESQMTAPSCSQTLHPNQIITSFSEQNYFEDQQSNLAVNNGFY
GNQVILPNGYQAFYEPQMRANFDQTNDQMKSFSGQNVCKGQENLLSGECSLSGYQTSGYR
CDQDLIVNPQVTSPIGGQPLSDFQTQTSSFDTALPGSKRTRPSAEDNLACPLETSPTSEE
TLYLGQMRTSVDQNFYSSQNGTPNIKESLDPQVTSFSLQAPYVGESSYPSCSPLIQKQPQ
KNSSASSLVQRQHPEMKLDFKTQSPVSQKDPSPLKLYFCTYKGCTKFYKRAYHLKEHQKK
HTDKRKYGCDEPGCTWSFFRLCDLNRHKEKHNGERFYACPLCSTNYSRLLYLKKHLEKKH
AQAQPTTAT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1854

Start on reference sequence 205

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Zfp352

Symbol Nomenclature :

Zfp352

SYNONYM :

2czf48

Full Nomenclature :

zinc finger protein 352

GENEID :

236537

Locus Tag :

RP23-354E5.1

GI :

147898090

GenBank Accession :

BC140425

Mouse Genome Informatics :

2387418

Vega :

OTTMUSG00000007775

Target GenBank:

NM_153102

Protein

SMART domain :

ZnF_C2H2 : zinc finger

UniProt :

A2AML7

Clone

Cloning Information :

265356

HIP Master Clone ID :

197454

IMAGE ID :

100014466

Original Clone ID :

FLH265356.01F

Organism

Species Specific ID:

236537

Genome

Chromosome :

Map Location :

4 C5|4

Ensembl :

ENSMUSG00000070902

Reagents

Labome :

Zfp352-siRNA