Gene:
Gene Symbol:
Lamc2
Gene Name:
laminin, gamma 2
Original Clone ID:
FLH265387.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080765
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3579nts
Open reading frame: 1 to 3579
ATGCCTGCGCTCTGGCTCAGCTGCTGCCTTGGTGTCGCGCTCCTGCTGCCCGCAGCCCAG
GCCACCTCCAGGAGGGAAGTCTGTGATTGCAATGGGAAGTCCAGGCAATGTGTCTTTGAT
CAGGAGCTCCATCGACAGACAGGCAGCGGGTTCCGTTGCCTCAACTGCAATGACAATACA
GCGGGGGTTCACTGCGAGCGGTGCAGGGAGGGGTTTTACCGACATCGAGACAGAGACCGC
TGCCTACCCTGCAACTGCCACTCAAAGGGTTCCCTCAGTGCTGGATGTGACAACTCTGGA
CAATGCAGGTGTAAGCCAGGTGTGACAGGACAAAGATGTGACCGATGTCAGCCAGGCTTC
CATATGCTCACCGATGCTGGATGCACCCGAGACCAGGGGCAACTAGATTCCAAGTGTGAC
TGTGACCCAGCTGGCATCTCTGGACCCTGTGATTCTGGCCGATGTGTCTGCAAACCAGCC
GTCACTGGAGAGCGCTGTGATAGGTGCCGACCAGGCTACTATCATCTGGACCGGGCAAAC
CCTGAGGGCTGTACCCAGTGTTTCTGCTATGGGCATTCAGCCAGCTGCCACGCCTCTGCC
GACTTCAGTGTCCACAAAATCACTTCAACTTTCAGTCAGGATGTGGATGGTTGGAAGGCG
GTTCAGAGAAACGGGGCACCTGCAAAACTCCACTGGTCACAGCGCCATCGGGACGTGTTT
AGTTCTGCCCGAAGATCAGACCCCGTCTATTTCGTGGCCCCTGCCAAATTCCTCGGTAAC
CAGCAAGTGAGTTACGGGCAGAGCCTGTCTTTTGACTACCGCGTGGACAGAGGAGGTAGA
CAGCCGTCTGCCTACGATGTGATCCTGGAAGGTGCTGGTCTACAGATCAGAGCTCCTCTG
ATGGCTCCAGGCAAGACACTTCCCTGTGGGATCACAAAGACTTACACATTCAGACTGAAT
GAACATCCAAGCAGTCACTGGAGTCCCCAGCTGAGTTATTTCGAATATCGAAGGTTACTG
CGGAACCTCACAGCCCTCCTGATCCGAGCTACGTACGGAGAATATAGTACAGGGTACATT
GATAACGTGACCCTGGTTTCAGCCCGCCCTGTCTCTGGAGCCCCAGCCCCTTGGGTTGAA
CGTTGTGTATGCCCTGCTGGGTACAAGGGACAATTCTGCCAGGAATGTGCTTCTGGTTAC
AAAAGAGATTCGGCAAGATTGGGGCCTTTTGGCGCCTGTGTTCCCTGTAACTGCCAAGGG
GGAGGGGCCTGTGATCCAGACACGGGAGATTGCTACTCGGGGGACGAGAATCCTGACATT
GAGTGTGCTGACTGTCCCATCGGTTTCTACAATGACCCACATGACCCCCGCAGCTGCAAG
CCATGTCCCTGTCACAATGGGTTCAGCTGTTCAGTGATGCCTGAGACAGAGGAGGTGGTG
TGTAACAACTGTCCCCCTGGGGTCACAGGTGCCCGCTGTGAGCTCTGTGCTGATGGCTTC
TTTGGGGATCCCTTTGGGGAACGTGGCCCAGTGAGGCCTTGTCAACGCTGCCAATGCAAC
AACAACGTGGACCCCAATGCCTCTGGGAACTGTGACCAGTTGACAGGCAGATGCTTGAAA
TGTATCTACAACACGGCCGGTGTCTACTGTGACCAGTGCAAAGCAGGTTACTTTGGAGAC
CCATTGGCTCCCAACCCAGCAGACAAGTGTCGAGCTTGCAACTGCAGCCCCATGGGCTCG
GAGCCTGGAGAGTGTCGAGGTGATGGCAGCTGTGTTTGCAAGCCAGGCTTTGGCGGCCTC
AACTGTGATCACGCAGCCCTAACCAGTTGTCCTGCTTGCTACAATCAAGTGAAGATTCAG
ATGGACCAGTTTACCCAGCAGCTCCAGAGCCTGGAGGCCCTGGTTTCAAAGGCTCAGGGT
GGTGGTGGTGGTGGTACAGTCCCCAGTGGGGAGCTGGAAGGCAGGATGCAGCAGGCTGAG
CAGGCCCTTCGAGACATTCTAAGAGAAGCTCAGATTTCAGAAGGGGCAATGAGAGCCCTT
GGCTTCCGGCTGGCCAAGGCAAGGAGCCAAGAGAACGACTACAAGACCCGCCTGGATGAC
CTCAAGATGACTGCAGAAAGGATCCGGGCCCTGGGCAGTCAGCATCAGAACAGAGTTCAG
GATACGAGCAGACTCATCTCTCAGATGCGCCTGAGTCTGGCAGGAAGCGAAGCTTCCCTG
CAAAACACTAATATCCATTCTTCTGAGCACTACGTGGGGCCGAATGGATTTAAAAGTCTG
GCTCAGGAGGCTACAAGATTGGCAGACAGCCACGCTGAGTCAGCTAACGCAATGAAGCAA
CTAGCAAGGGAAACTGAGGACTACTCCAAACAAGCACTTTCATTGGCCCGCAAGGCTCTG
AGTGGAGGAGGCGGAAGTGGCGTCTTGGACAGCTCCGTGGTACAAGGTCTTATGGGAAAA
TTAGAGAAAACCAAGTCCCTGAGCCAGCAGCTGTCAAGGGAGGGCACCCAAGCCGACATT
GAAGCTGATAGGTCGTATCAGCACAGTCTCCGCCTCCTGGATTCTGCCTCTCAGCTTCAG
GGAGTCAGTGATCTGTCCTTTCAGGTGGAAGCAAAGAGGATCAGACAAAAGGCTGATTCT
CTCTCAAACCTGGTGACCAGACAAACGGATGCATTCACGCGTGTGCGAAACAATCTGGGG
AACTGGGAAAAAGAAACACGGCAGCTTTTACAGACTGGAAAGGATAGGAGACAGACTTCA
GATCAGCTGCTTTCCCGTGCCAACCTTGCTAAAAACAGAGCCCAAGAAGCGCTAAGTATG
GGCAATGCCACTTTTTATGAAGTTGAGAACATCCTGAAGAACCTCCGAGAGTTTGATCTG
CAGGTTGAAGACAGAAAAGCAGAGGCTGAAGAGGCCATGAAGAGACTCTCCTCTATTAGC
CAGAAGGTTGCAGATGCCAGTGACAAGACCCAGCAAGCAGAAACGGCCCTGGGGAGCGCC
ACTGCCGACACCCAACGGGCAAAGAACGCAGCTAGGGAGGCCCTGGAGATCAGCAGCGAG
ATAGAGCAGGAGATAGGGAGTCTGAACTTGGAAGCTAATGTGACAGCAGATGGGGCCTTG
GCCATGGAGAAGGGACTGGCCACTCTGAAGAGCGAGATGAGAGAGATGGAGGGGGAGCTG
GCCAGAAAGGAGCTGGAGTTTGACACGGATAAGGACACGGTGCAGCTGGTGATTACTGAA
GCCCAGCAAGCTGATGCCAGAGCCACGAGTGCCGGAGTTACCATCCAAGACACACTCAAC
ACATTGGACGGCATCCTACACCTCATAGACCAGCCTGGCAGTGTGGATGAAGAAGGGATG
ATGCTATTAGAACAAGGGCTTTTCCAAGCCAAGACCCAGATCAACAGTCGACTTCGGCCC
TTGATGTCTGACCTGGAGGAGAGGGTGCGTCGGCAGAGGAACCACCTCCATCTGCTGGAG
ACCAGCATAGATGGAATTCTAGCTGATGTGAAGAACCTGGAGAACATTCGAGACAACCTG
CCCCCAGGCTGCTACAATACCCAAGCTCTTGAGCAACAG
Protein sequence predicted based on nucleotide sequence
MPALWLSCCLGVALLLPAAQATSRREVCDCNGKSRQCVFDQELHRQTGSGFRCLNCNDNT AGVHCERCREGFYRHRDRDRCLPCNCHSKGSLSAGCDNSGQCRCKPGVTGQRCDRCQPGF HMLTDAGCTRDQGQLDSKCDCDPAGISGPCDSGRCVCKPAVTGERCDRCRPGYYHLDRAN PEGCTQCFCYGHSASCHASADFSVHKITSTFSQDVDGWKAVQRNGAPAKLHWSQRHRDVF SSARRSDPVYFVAPAKFLGNQQVSYGQSLSFDYRVDRGGRQPSAYDVILEGAGLQIRAPL MAPGKTLPCGITKTYTFRLNEHPSSHWSPQLSYFEYRRLLRNLTALLIRATYGEYSTGYI DNVTLVSARPVSGAPAPWVERCVCPAGYKGQFCQECASGYKRDSARLGPFGACVPCNCQG GGACDPDTGDCYSGDENPDIECADCPIGFYNDPHDPRSCKPCPCHNGFSCSVMPETEEVV CNNCPPGVTGARCELCADGFFGDPFGERGPVRPCQRCQCNNNVDPNASGNCDQLTGRCLK CIYNTAGVYCDQCKAGYFGDPLAPNPADKCRACNCSPMGSEPGECRGDGSCVCKPGFGGL NCDHAALTSCPACYNQVKIQMDQFTQQLQSLEALVSKAQGGGGGGTVPSGELEGRMQQAE QALRDILREAQISEGAMRALGFRLAKARSQENDYKTRLDDLKMTAERIRALGSQHQNRVQ DTSRLISQMRLSLAGSEASLQNTNIHSSEHYVGPNGFKSLAQEATRLADSHAESANAMKQ LARETEDYSKQALSLARKALSGGGGSGVLDSSVVQGLMGKLEKTKSLSQQLSREGTQADI EADRSYQHSLRLLDSASQLQGVSDLSFQVEAKRIRQKADSLSNLVTRQTDAFTRVRNNLG NWEKETRQLLQTGKDRRQTSDQLLSRANLAKNRAQEALSMGNATFYEVENILKNLREFDL QVEDRKAEAEEAMKRLSSISQKVADASDKTQQAETALGSATADTQRAKNAAREALEISSE IEQEIGSLNLEANVTADGALAMEKGLATLKSEMREMEGELARKELEFDTDKDTVQLVITE AQQADARATSAGVTIQDTLNTLDGILHLIDQPGSVDEEGMMLLEQGLFQAKTQINSRLRP LMSDLEERVRRQRNHLHLLETSIDGILADVKNLENIRDNLPPGCYNTQALEQQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
309
End on reference sequence
3890
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
