DNASU Plasmid | D030010E02Rik (M. musculus )<br>In pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Cdcp2

Gene Name: CUB domain containing protein 2

Original Clone ID:

FLH265430.01F

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00080808

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1281nts

Open reading frame: 1 to 1281

ATGCTAGCTGAGCTGGGGGCCTGCCTACTGCTGGCAATGGTACTGTTGGACTCCGATCCT
GGGACCCAAGCCATGGAAGGTGTCAAGTGTGGGGGTGTGCTCTCAGCGCCATCTGGGAAC
TTCTCCAGCCCAAACTTCCCAAGTCTGTACCCCTACAACACGGAGTGCAGCTGGCTGATC
GTGGTGGCCGAGGGCTCCTCTGTGCTGCTCACCTTCCACGCCTTTGACCTGGAATACCAT
GACACCTGTGGCTTTGACTTCCTGGAGATCTACAACGGGGCCTCTGGAGACAAGGGCAAT
CTGCTGGGCAGGTTCTGTGGCCAGGTGCCCCCACCGCCCTTCACCTCCTCCTGGCACGTC
ATGTCTGTCGTCTTTCACTCAGACAAGCACGTGGCCAGCCGAGGCTTTTCTGCAGGCTAC
CAGAAAGATGTGTGCGGCGGTGTCTTGACCGGCCTGTCAGGGGTTCTCAGCAGCCCTGAG
TACCCCAACAATTACCCCAACAATGTGGAGTGCCACTGGCTGATCCGCGCCTCAGGACCT
GCTGCTGTCAAGTTGGTGTTCGTGGACTTCCAGGTTGAGGGCAGCGACGAATGCATGTAT
GACTACGTAGCTGTGCTTGGAGCGCCTGGCCCTGCCCACGGGCACCACTACTGTGGCCGC
ACCAGACCCCCCACCTTCGTGTCCCTGGGCCATGAGCTGCAGGTGGTCTTCAAGTCTGAC
TTCAACATTGGAGGCCGGGGCTTCAAGGCCCACTACTTCTCAGGAGAATGCCAGGAGGTG
TTCACAGCTGTGCGTGGAAACTTCTCCAGCCCACAGTACCCTGGCGCCTACCCCAACAAT
ATCCGTTGCCACTGGACCATCCGCCTGCCCCCCGGCTACCGGGTCAAGGTCTTCATCCTG
GACCTGGGCTTGGAGGAGCCCAATAGCCTAACCAGAACCTGTGACTTCGACCATCTGGCA
GCCTTTGATGGGGCCAGTGAGGAGGCACAGCTGCTGGGGAAGTGGTGTGGGCATCACCTG
CCGCCGCCTGTCACCTCGAGTCGCAACCAGGTCCTGCTCCTGCTGCACACTGACCGCAGC
ACCTCCGGCAGGGGCTTCTCCGTGGCCTACATCGGAGGTCAGCTGGGTGTGTGTCCTTGG
GAACTCTGCGGCCCCTTCCTGTGTCCACTGAGACACAGCCCCTCCACTGACTCCTCCAGA
CCCCACTCAGTCGCCTACCCTGGGGACCTTGGAGGCCACTCCAGTACGGCCCTCATTTAT
TTCCACTGTCACCTCTTTTCA

Protein sequence predicted based on nucleotide sequence

MLAELGACLLLAMVLLDSDPGTQAMEGVKCGGVLSAPSGNFSSPNFPSLYPYNTECSWLI
VVAEGSSVLLTFHAFDLEYHDTCGFDFLEIYNGASGDKGNLLGRFCGQVPPPPFTSSWHV
MSVVFHSDKHVASRGFSAGYQKDVCGGVLTGLSGVLSSPEYPNNYPNNVECHWLIRASGP
AAVKLVFVDFQVEGSDECMYDYVAVLGAPGPAHGHHYCGRTRPPTFVSLGHELQVVFKSD
FNIGGRGFKAHYFSGECQEVFTAVRGNFSSPQYPGAYPNNIRCHWTIRLPPGYRVKVFIL
DLGLEEPNSLTRTCDFDHLAAFDGASEEAQLLGKWCGHHLPPPVTSSRNQVLLLLHTDRS
TSGRGFSVAYIGGQLGVCPWELCGPFLCPLRHSPSTDSSRPHSVAYPGDLGGHSSTALIY
FHCHLFS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2102

Start on reference sequence 819

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Cdcp2

Symbol Nomenclature :

Cdcp2

SYNONYM :

D030010E02Rik

Designation :

CUB domain-containing protein 2

Full Nomenclature :

CUB domain containing protein 2

GENEID :

242603

Locus Tag :

RP23-423N6.1

GI :

148921917

GenBank Accession :

BC146400

Mouse Genome Informatics :

3045328

Vega :

OTTMUSG00000008474

Target GenBank:

NM_172873

Protein

SMART domain :

CUB : Domain first found in C1r, C1s, uEGF, and bone morphogenetic protein.

UniProt :

Q8BQH6

Clone

Cloning Information :

265430

HIP Master Clone ID :

197528

IMAGE ID :

100015100

Original Clone ID :

FLH265430.01F

Organism

Species Specific ID:

242603

Genome

Chromosome :

Map Location :

4 C7|4

Ensembl :

ENSMUSG00000047636

Reagents

Labome :

Cdcp2-siRNA

D030010E02Rik (M. musculus )In pENTR223.1 (Gateway donor/master vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Organism

Genome

Reagents

D030010E02Rik (M. musculus )
In pENTR223.1 (Gateway donor/master vector)