DNASU Plasmid | Mrcl (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Clec18a

Gene Name: C-type lectin domain family 18, member A

Original Clone ID:

FLH265499.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00080877

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1434nts

Open reading frame: 1 to 1434

ATGGCTCGGTCTCTTGCTTCTGCTCCTGTCTCTCCTTGGCATCACATGGACAGAGGTTCA
ACCACCCCAGCCAAAGCAAGATCCCACTCTGCAAGCTACCACTGCCCCTCCTCATCTCAG
ATCTGTGTTCCTGGGGTGGGGTCACCCTCAGCTGGCTATGGCGATGTCCCAGGAGCCTCT
GGTCATCGGCCTAACCTTCTCTCCTGGCCTCCAGCCCTGAGCAGGAAGGAGAGTTTCTTA
ATCCTCACCGCACACAACCGACTGCGCAGCCGGGTCCACCCTCCTGCAGCCAACATGCAG
AGAATGGACTGGAGCGAGAGCCTGGCTCAGCTAGCTGAAGCCAGGGCAGCCCTTTGTGTC
ACTTCAGTCACCCCAAACCTGGCATCTACCCCAGGGCACAACTCACATGTGGGCTGGAAT
GTGCAGCTAATGCCCATGGGCTCAGCATCCTTTGTCGAAGTGGTCAACCTCTGGTTTGCT
GAAGGGCTGCAGTACAGACATGGGGATGCTGAGTGTGCCCACAATGCCACCTGTGCCCAC
TACACACAACTTGTGTGGGCTACCTCCAGCCAGCTGGGCTGTGGGCGGCAGCCATGCTTT
GTGGACCAGGAAGCCATGGAAGCCTTTGTCTGTGCCTACTCCCCTGGAGGCAACTGGGAT
ATAAATGGGAAGACAGTCGCCCCTTACAAGAAAGGCACCTGGTGCTCCCTCTGCACAGCC
AGGGTCTCGGGCTGCTTCAAGGCCTGGGATCACGCAGGGGGGCTCTGTGAGGTTCCCAGG
AACCCATGCCGCATGAGCTGCCGAAATCTCGGACATCTCAACATTAGCACTTGTCGCTGT
CACTGTCAACCTGGCTACACAGGGAGGTACTGCCAAGTGCGCTGCAGTGTACAGTGTGTG
CACGGCCAATTCCGGAAAGAAGAATGTTCCTGTATCTGTGATGTCGGCTATGGGGGAGCC
CAGTGTGCCACCAAGGTGGGCTTTCCCTTCCACACCTGTGACCTGAGGATTGACGGAGAC
TGCTTCATGGTGTCTCCTGAGGCAGACACCTACTATGGAGCCAAGATGAAATGTCAGGGA
AAAGGTGGGGTGCTAGCCCAGATCGAGAGCCAGAAAGTGCAAGACATCCTTGCCTTCTAC
CTGGGCCGCCTGGAGACCACCAACGAGGTGACTGACAGTGACTTTGAGACCAAGAATTTC
TGGATTGGGCTCACCTACAAGGCAGCTAAGGATTCCTTCCGCTGGACTACTGGAGAACAC
CAGTCCTTCACCAGTTTTGCCTTCGGGCAGCCTGACAACCAGGGGTTTGGGAATTGTGTG
GAAATGCAGGCATCTGCTGCCTTCAACTGGAATGACCAACGTTGTAAAACTCGAAACCGT
TATATCTGCCAGTTTGCTCAGAAGCACTATTCCCGGTGGGAGCCAGGGCCCTAA

Protein sequence predicted based on nucleotide sequence

MARSLASAPVSPWHHMDRGSTTPAKARSHSASYHCPSSSQICVPGVGSPSAGYGDVPGAS
GHRPNLLSWPPALSRKESFLILTAHNRLRSRVHPPAANMQRMDWSESLAQLAEARAALCV
TSVTPNLASTPGHNSHVGWNVQLMPMGSASFVEVVNLWFAEGLQYRHGDAECAHNATCAH
YTQLVWATSSQLGCGRQPCFVDQEAMEAFVCAYSPGGNWDINGKTVAPYKKGTWCSLCTA
RVSGCFKAWDHAGGLCEVPRNPCRMSCRNLGHLNISTCRCHCQPGYTGRYCQVRCSVQCV
HGQFRKEECSCICDVGYGGAQCATKVGFPFHTCDLRIDGDCFMVSPEADTYYGAKMKCQG
KGGVLAQIESQKVQDILAFYLGRLETTNEVTDSDFETKNFWIGLTYKAAKDSFRWTTGEH
QSFTSFAFGQPDNQGFGNCVEMQASAAFNWNDQRCKTRNRYICQFAQKHYSRWEPGP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1634

Start on reference sequence 201

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Clec18a

Symbol Nomenclature :

Clec18a

SYNONYM :

Mrcl

Designation :

C-type lectin domain family 18 member A|mannose receptor-like protein

Full Nomenclature :

C-type lectin domain family 18, member A

GENEID :

353287

GI :

148922424

GenBank Accession :

BC146578

Mouse Genome Informatics :

2672935

Target GenBank:

NM_181549

Protein

SMART domain :

CLECT : C-type lectin (CTL) or carbohydrate-recognition domain (CRD)

SMART domain :

SCP : SCP / Tpx-1 / Ag5 / PR-1 / Sc7 family of extracellular domains.

Clone

Cloning Information :

265499

HIP Master Clone ID :

197597

IMAGE ID :

100014801

Original Clone ID :

FLH265499.01X

Organism

Species Specific ID:

353287

Genome

Chromosome :

Map Location :

8 E1|8

Ensembl :

ENSMUSG00000033633

Reagents

Labome :

Clec18a-antibody