DNASU Plasmid | Rasgrp4 (M. musculus )<br>In pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Rasgrp4

Gene Name: RAS guanyl releasing protein 4

Original Clone ID:

FLH265521.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00080899

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2022nts

Open reading frame: 1 to 2022

ATGAACCGGAAAGACATCAAAAGGAAGTCTCATCAGGAATGCTCTGGGAAGGCAGGAGGG
CGGGGTCGGTCACGTCAGGCCCGCCGCCACAAGACGTGCCCCACCCCCCGGGAAATCAGC
AAGGTCATGGCGTCCATGAATCTGGGAGTGCTGAGTGAGAGCAGCTGCAGCGAAGATGAG
CTATTGGAGGAATGTATCCGCTGCTTTGACTCGGCTGGCAGCCTGCGCCGAGGGGACCAC
ATTCTCAAGATGGTGCTCACGATGCACAGCTGGGTGCTGCCATCCTCAGAGCTCGCTGCC
CGTCTGCTGACTTCGTATCAGAAGGCTGCCAAAGATGCCCAAGAGCTAAGACAGCTACAG
ATCTGTTACTTGGTCAGGTACTGGCTGACCCATCACCATGAGGCAGTGCACCAGGAACCC
CAGCTGGAAGCAGTCATAAGCCGGTTTTGGACCACTGTCGCTCAGGAGGGCAACATGGCC
CAAAGGAGCCTGGGAGATGCCTCCAGCCTGCTAAGTCCTGGAGGGCCCGGTCCCCCACCT
CCCATGAGCAGCCCAGGCCTGGGCAAGAAGCGGAAAGTGTCATTGCTATTCGATCACCTG
GAGACAGAGGAGCTGGCACAACACCTCACTTACCTGGAGTTCCGGTCTTTCCAGGCTATC
ACACCCCAAGACCTGCGGGGCTATGTTTTGCAGGGTTCAGTGAGAGGTTGTCCAGCTCTG
GAAGGTTCTGTGGGTCTGAGCAACAGTGTGTCCCGCTGGGTGCAGGTCATGGTGCTGAGT
CGTCCTGGGCCTGCACAACGTGCCCAGGTCCTGGACAAATTCATTCGTGTGGCACAGAGG
CTTCACCAGCTGCAGAATTTCAACACACTGATGGCGGTCACGGGGGGTCTGTGTCATAGC
GCCATCTCCAGACTCAAGGACTCCCACGTTCATCTGAGCCCTGACAGCACAAAGGCCCTG
CTGGAGCTGACAGAGCTCCTCTCATCCCACAACAACTATGCCCACTACCGCCGCACCTGG
GCCGGCTGTACTGGCTTCCGGCTGCCAGTACTGGGTGTGCACCTCAAGGACCTGGTATCT
CTATATGAGGCTCATCCAGACAGATTGCCAGATGGCCGCCTGCACCTACCCAAGCTGAAT
AGCCTCTATCTACGGCTGCAGGAGCTGATGGCGCTCCAGGGACAGCATCCTCCCTGCAGT
GCCAATGAGGACCTGCTGCACCTGCTGACGCTCTCCCTGGATCTCTTCTACACGGAAGAT
GAGATCTACGAGCTTTCTTATGCCCGGGAACCACGCTGTCCCAAGAGTCTGCCACCGTCC
CCCTTCAAAGCACCAGTGGTGGTAGAGTGGGCCCAGGGTGTGACACCAAAGCCAGACAGC
GTGACTCTGGGTCAGCATGTGGAACAGCTGGTGGAGTCCGTGTTCAAGAACTACGACCCA
GAAGGCCGTGGCTCCATCTCTCTGGAGGACTTTGAGCGGCTGTCAGGCAACTTCCCGTTC
GCCTGCCATGGGCTTCACCCACCTCCCCGCCATGGGAGTGGCTCCTTCAGCAGAGAGGAG
CTGACCAAGTACCTGCTCCATGCCAGTGCCATCTGCTCCAAGCTGGGCCTGGCCTTCCTG
CACGCCTTCCAGGAGGTCACCTTCCGAAAGCCCACGTTCTGTCACAGCTGCAGCGGCTTC
CTCTGGGGGGTCACCAAGCAAGGCTATCGCTGTCGGGACTGTGGGCTGTGTTGTCACAGA
CACTGCAGGGATCAAGTGAGAGTGGAGTGTAAGAAGAGGCCAGAGACTAAGGGTGACCCG
GGCCCCCCAGGTGCACCTGTGCCAGCCACATCACTTCCTCCTGCCAACTGTGGCTCAGAG
GAAAGTCTCTCCTATACACTCTCCCCGGATCCGGAGTCTGGTTGCCACCTTCGCCATGCT
TGGACCCAGACGGAATCCTCACACTCTTCCTGGGAGCCAGAGGTGGTGCCCTGCCCAGCA
CGGGTCTTACCATCCAGAGCTTCCTCGAAGCCCAGCGTCTAA

Protein sequence predicted based on nucleotide sequence

MNRKDIKRKSHQECSGKAGGRGRSRQARRHKTCPTPREISKVMASMNLGVLSESSCSEDE
LLEECIRCFDSAGSLRRGDHILKMVLTMHSWVLPSSELAARLLTSYQKAAKDAQELRQLQ
ICYLVRYWLTHHHEAVHQEPQLEAVISRFWTTVAQEGNMAQRSLGDASSLLSPGGPGPPP
PMSSPGLGKKRKVSLLFDHLETEELAQHLTYLEFRSFQAITPQDLRGYVLQGSVRGCPAL
EGSVGLSNSVSRWVQVMVLSRPGPAQRAQVLDKFIRVAQRLHQLQNFNTLMAVTGGLCHS
AISRLKDSHVHLSPDSTKALLELTELLSSHNNYAHYRRTWAGCTGFRLPVLGVHLKDLVS
LYEAHPDRLPDGRLHLPKLNSLYLRLQELMALQGQHPPCSANEDLLHLLTLSLDLFYTED
EIYELSYAREPRCPKSLPPSPFKAPVVVEWAQGVTPKPDSVTLGQHVEQLVESVFKNYDP
EGRGSISLEDFERLSGNFPFACHGLHPPPRHGSGSFSREELTKYLLHASAICSKLGLAFL
HAFQEVTFRKPTFCHSCSGFLWGVTKQGYRCRDCGLCCHRHCRDQVRVECKKRPETKGDP
GPPGAPVPATSLPPANCGSEESLSYTLSPDPESGCHLRHAWTQTESSHSSWEPEVVPCPA
RVLPSRASSKPSV*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2109

Start on reference sequence 88

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Rasgrp4

Symbol Nomenclature :

Rasgrp4

Designation :

RAS guanyl-releasing protein 4

Full Nomenclature :

RAS guanyl releasing protein 4

GENEID :

233046

GI :

148921861

GenBank Accession :

BC146526

Mouse Genome Informatics :

2386851

Vega :

OTTMUSG00000033130

Target GenBank:

NM_145149

Protein

SMART domain :

C1 : Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

SMART domain :

RasGEF : Guanine nucleotide exchange factor for Ras-like small GTPases

UniProt :

Q8BTM9

Clone

Cloning Information :

265521

HIP Master Clone ID :

197619

IMAGE ID :

100014918

Original Clone ID :

FLH265521.01X

Organism

Species Specific ID:

233046

Genome

Chromosome :

Map Location :

7 B1|7 16.94 cM

Ensembl :

ENSMUSG00000030589

Reagents

Labome :

Rasgrp4-antibody

Rasgrp4 (M. musculus )In pENTR223.1 (Gateway donor/master vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Organism

Genome

Reagents

Rasgrp4 (M. musculus )
In pENTR223.1 (Gateway donor/master vector)