Gene:
Gene Symbol:
Fsd2
Gene Name:
fibronectin type III and SPRY domain containing 2
Original Clone ID:
FLH265545.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080923
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2148nts
Open reading frame: 1 to 2148
ATGGAGGAGGAAGCAGAGGAGGAATCCGGGCTGGGCAGGTCGGCAGCCCCCAAAGACTTC
CACTTTTATCACATGGACCTGTATGACTCTGAAGATAGACTGCAGCTCTTCCCAGGAGAC
AGCAGCAGAATCAGGAGGGAAGTAACCCAAGCCGAAGATGACCGTGAATTAGGGGATGAA
TTTGTGGATGAACACAGACTGGGGACTCTGGGGTATCCTTCTTACGGGATGAGGAGGAGG
GACCCAGGCAGGGAGCCGAGAGACTGGGGTGAGGCAGCTGAGGCGGAGGACCTGGGCTAC
GGAGGAGGGCAAGGCCCTCTTGACCAGTGCCAAGACCTCCGGGAAGCCTACAGGTACACA
CATGGCCGTGCCAGCGAGGAGTATGAATGCTATGTCATCCCAGAGGAGGAAGACGAAGAA
GAGCCTGCTGATGTCTTCTGCATCACTTGCAAAACTCCAGTAAGAACTGTTGAGAAGGAT
TTTGATACACACAAGGAACACGAAGTAACCCCAATCAGCAAAGCTCTGGAACATGCCAAG
GATGAGGTTCACAAAAATATGTGCAAATTGGAACAGCAGATAATTGAGATGGAAAATTTC
GCTAGCCACCTGGAAGAAGTGTTCATTACTGTGGAGGAGAATTTTGGAAGACAGGAACAA
AACTTTGAGTGCCATTACAATGGGATCTTGGAAACCCTCGCTCAAAAGTATGAAGAAAAA
ATTCAAGCTCTGGGGGAGAAAAAGAGAGAGAAACTGGAAGCCTTATACGGACAACTGGTC
AGCTGTGGAGAGAACCTCGATGCCTGCAGGGAGCTGATGGAAACCGTAGAGGAGATGTGT
CATGAGGAAAAGGTGGAGTTCTTAAAGGATGCAGTGGCTATGACTGACAGACTGGGGAAA
TTCCTGAAAACCAAAACTGATGTGGAGCTCTCTGCACAACCTGAGTTTGAAGACCAGACC
TTGGACTTCTCTGATGTGGAGCAGCTGATGGACGCCATCAATACCATTCCAGCGCCCTCT
GCTCCAGTGATAAATCCCCAGGCACCCAACTCCGCCACCGGCTCCTCAGTGCGTGTATGC
TGGAGCTTATATTCTGACGACACCGTGGAGAGCTACCAGCTGTCCTATAGGCCGGTGCAG
GACAGCTCATCTGGCAAGGACCGAGCAGAATTCACCATGATGGTCAAAGAAACATATTGC
TCAGTGACAAATCTTGAGCCAAATACTCAGTATGAGTTTTGGGTCATAGCTCAGAACAGG
ACTGGCCCCAGCCCCTGCAGTGAGCACGCAGTGTATATGACAGCCCCCTCTCCCCCCTCT
ATAAAAACCGAGGCAATCAGAAGCTGTGAAGAGGCTGTGCTGATTTGCTGGGAGTCTGGG
AACCTGAATCCGGTGGACTCCTACACGGTGGAGCTAATCCAGGCAGAGACGCCAGAAGCC
TCGGGGGTAACTGAATCTGTCGTAGGCATCCCAACCTGTGAATCCCTGATACAGCTGCAG
CCTAGACACAGCTATACCATCTATGTGCGAGCCCTCAACGTGGGAGGCACCAGTGCCAGA
AGTGAGCCTGCTACAGTGCACACCACAGGAAGCTACTTCCAATTGAACAAAGACACCTGC
CATCCCTGGCTGACCATTTCTGAAGATGGGTTTACGGTAGTTCGAAGTGAGAAGAAAAGT
TTCAGAAAGGAGCTGCCGCCGAGCAAGACGCAATTTACCAGGTGTGTTGCTGTCATGGGG
AATCTGATTCCGGTCCGAGGGCGCCATTACTGGGAAGTGGAGGTGGCTGAGCATTTGGAC
TACACTGTGGGTGTGGCCTGTGAAGATGTCCCTAAGCAGGAAGACCTGGGAGCAAACTCC
TTGTCCTGGTGCATGAGGCATACATTTGTTTCCAAAAGGCACCGGTATGAGTTTCTGCAC
AACAAGATGACTCCAGACATCAGGATCACAGTTGCTCCGAGGAAGATTGGCGTGCTGCTG
GACTATGAGAACGCCAAGCTGTCCTTTTTCAATGTGGACATTGCTCAGCACCTGTACACA
TTTAGCTGTCAGCTCCACCAGTTTGTGCATCCCTGCTTTTCCCTGGAAAAGTCTGGATGC
CTAAAGATCTGCAATGGCATTTCAATGCCAAAGCATGTCACTTTCTTT
Protein sequence predicted based on nucleotide sequence
MEEEAEEESGLGRSAAPKDFHFYHMDLYDSEDRLQLFPGDSSRIRREVTQAEDDRELGDE FVDEHRLGTLGYPSYGMRRRDPGREPRDWGEAAEAEDLGYGGGQGPLDQCQDLREAYRYT HGRASEEYECYVIPEEEDEEEPADVFCITCKTPVRTVEKDFDTHKEHEVTPISKALEHAK DEVHKNMCKLEQQIIEMENFASHLEEVFITVEENFGRQEQNFECHYNGILETLAQKYEEK IQALGEKKREKLEALYGQLVSCGENLDACRELMETVEEMCHEEKVEFLKDAVAMTDRLGK FLKTKTDVELSAQPEFEDQTLDFSDVEQLMDAINTIPAPSAPVINPQAPNSATGSSVRVC WSLYSDDTVESYQLSYRPVQDSSSGKDRAEFTMMVKETYCSVTNLEPNTQYEFWVIAQNR TGPSPCSEHAVYMTAPSPPSIKTEAIRSCEEAVLICWESGNLNPVDSYTVELIQAETPEA SGVTESVVGIPTCESLIQLQPRHSYTIYVRALNVGGTSARSEPATVHTTGSYFQLNKDTC HPWLTISEDGFTVVRSEKKSFRKELPPSKTQFTRCVAVMGNLIPVRGRHYWEVEVAEHLD YTVGVACEDVPKQEDLGANSLSWCMRHTFVSKRHRYEFLHNKMTPDIRITVAPRKIGVLL DYENAKLSFFNVDIAQHLYTFSCQLHQFVHPCFSLEKSGCLKICNGISMPKHVTFF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
184
End on reference sequence
2334
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
