Gene:
Gene Symbol:
Pikfyve
Gene Name:
phosphoinositide kinase, FYVE finger containing
Original Clone ID:
FLH265559.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080936
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6156nts
Open reading frame: 1 to 6156
ATGGCCACAGATGACAAGAGTTCCCCGACACTGGACTCTGCTAATGATTTGCCTCGCTCT
CCTGCCAGTCCTTCTCACCTCACTCACTTTAAACCCTTGACTCCTGACCAGGATGAGCCC
CCCTTCAAGTCAGCATATAGTTCTTTTGTAAACCTTTTTCGTTTTAACAAAGAGCGAGGA
GAAGGGGGCCAAGGAGAGCAGCAGTCTCCGAGTTCAAGTTGGGCCAGCCCTCAGATCCCT
TCAAGAACACAGTCTGTGAGGTCGCCTGTACCTTATAAAAAACAGCTTAATGAGGAGCTC
CACCGGCGCTCTTCAGTGTTAGAGAACACTTTGCCACATCCTCAGGAGAGCACAGACTCC
AGAAGGAAAGCAGAACCAGCCTGTGGAGGTCATGACCCACGTACAGCTGTTCAGCTTCGA
AGCCTCAGCACAGTATTGAAACGCCTCAAAGAAATCATGGAAGGAAAAAGCCAGGACAGT
GACCTGAAGCAATATTGGATGCCAGATAGCCAGTGTAAAGAGTGCTATGACTGCAGTGAA
AAGTTTACAACATTTAGGCGCAGACACCATTGCAGACTGTGTGGGCAGATTTTCTGCAGT
CGTTGTTGTAATCAAGAAATCCCTGGAAAATTTATGGGCTATACAGGAGACCTCCGAGCA
TGCACCTACTGTAGAAAAATAGCCTTAAGTTATGCTCATTCTACAGACAGTAATTCCATT
GGGGAAGACTTGAATGCTCTTTCAGATTCAACTTGCTCTGTGTCTATACTTGATCCAAGC
GAACCTCGGACACCAGTTGGGAGTAGAAAAGCCAGTCGTAACATATTCTTAGAGGATGAT
TTAGCCTGGCAAAGCTTGATTCATCCGGATTCCTCAAATAGTGCTCTTTCAACAAGACTC
GTATCTGTTCAAGAGGATGCTGGGAAGTCTCCTGCTCGAAACAGATCAGCCAGCATTACT
AATCTGTCACTGGATCGGTCTGGTTCTCCTATGGTTCCTTCATATGAGACATCTGTCAGT
CCCCAGGCTAACCGAAACTACATTAGGACAGAGACGACTGAGGATGAACGCAAAATTCTT
CTGGACAGTGCTCAGTTAAAGGATCTGTGGAAGAAAATCTGCCATCACACCAGTGGGATG
GAATTTCAAGATCACCGTTACTGGTTGAGAACACATCCCAACTGCATTGTAGGGAAGGAA
TTAGTCAACTGGCTAATCAGAAATGGACACATCGCTACAAGGGCACAAGCTATAGCAATT
GGACAAGCAATGGTTGATGGACGTTGGTTGGATTGTGTTAGTCATCATGATCAGCTTTTC
AGGGACGAATATGCGTTGTATAGACCACTTCAGAGTACAGAATTTTCTGAGACACCTTCT
CCAGACAGTGACTCTGTGAACTCTGTGGAAGGACACTCCGAGCCATCCTGGTTTAAAGAC
ATAAAATTTGATGACAGTGACACAGAACAGATTGCTGAAGAAGGTGACGATAATTTGGCT
AAGTATTTGGTTTCTGACACTGGAGGACAGCAGCTCTCAATAAGTGATGCCTTCATCAAA
GAGTCCTTATTTAATCGACGAGTAGAGGAAAAATCCAAAGAGCTGCCTTTTACCCCTTTG
GGCTGGCATCATAACAACCTGGAACTCTTGCGAGAGGAGAATGAGGAGAAGCAAGCCATG
GAAAGGCTGCTTTCAGCTAATCATAACCACATGATGGCCCTACTCCAGCAGTTGCTTCAA
AACGAGTCATTGTCATCGTCTTGGAGGGACATCATTGTGTCACTAGTCTGCCAGGTTGTT
CAGACAGTCCGACCTGATGTCAAGCACCAGGATGATGACATGGATATCCGTCAGTTTGTC
CATATCAAGAAGATCCCAGGTGGAAAGAAATTTGACTCTGTGGTTGTCAATGGCTTTGTT
TGTACCAAGAACATTGCACATAAAAAGATGAATTCCTGTATTAAAAACCCCAAAATCCTT
CTGTTGAAGTGTTCTATTGAGTATCTCTATAGAGAAGAAACTAAGTTTACCTGCATTGAT
CCTATTGTGCTTCAGGAAAGGGAATTCTTGAAGAATTATGTTCAACGAATAGTTGATGTT
CGACCCACATTGGTTCTTGTTGAGAAAACAGTGTCTCGGATTGCTCAGGACATGTTACTG
GAACATGGCATTACTCTGGTCATTAATGTAAAGTCACAAGTTTTAGAAAGAATCAGTCGA
ATGACCCAAGGTGATTTAGTGGTGTCCATGGACCAGCTGCTCACCAAACCCCACTTGGGC
ACTTGCCACAAATTTTATATGCAGATATTTCAGCTGCCTAATGAACAAACCAAAACACTG
ATGTTTTTTGAAGGCTGTCCACAGCATCTAGGCTGCACAATCAAGCTCAGAGGAGGCTCT
GATTATGAGCTGGCTCGAGTTAAGGAGATCCTAATATTTATGATCTGTGTAGCTTATCAT
TCTCAGCTAGAAATCTCTTTTCTCATGGATGAGTTCGCTATGCCTCCAACATTAATGCAA
AGCCCTTCATTCCATCTTCTGACGGAGGGACGAGGTGAAGAGGGAGCCTCTCAGGAGCAG
GTCAGTGGCAGCTCCCTTCCTCAGGATCCTGAGTGCCCTCGTGAGGCCCTGTCTTCTGAG
GATAGCACTTTGTTGGAATCAAGGACTGTGCTAGAGAAGGGTGAACTAGACAATAAAAGT
ATTCCACAAGCTGTTGCCTCTTTGAAGCATCAAGATTATACCACCCCCACTTGCCCAGCA
GGTATTCCCTGTGCTCTTTTTGCATTGGTACCAGAGTCATTGTTGCCTCTCCATATGGAT
CAACAGGATGCCGTAGGAAATGAACAGCCAGAGACTTCACAGCAAACGGATGAGCAACAG
GATCCCAAAAGCCAGATGAAAGCTTTTAGAGACCCTTTACAGGATGACACTGGAATGTAC
GTTACTGAGGAAGTCACCTCCTCTGAAGATCAACGAAAGACTTATGCCTTGACATTTAAA
CAGGAGTTAAAAGATGTAATCCTCTGTATCTCTCCAGTTATTACATTCCGTGAACCTTTC
CTTTTAACTGAAAAGGGGATGAGATGCTCAACTCGAGATTATTTTCCAGAGCAGATTTAC
TGGTCTCCTCTTCTCAACAAAGAGGTGAAGGAAATGGAGAGCAGGAGGAAGAAACAGCTG
CTCAGGGATCTCTCTGGACTTCAGGGCATGAATGGCAGTGTTCAGGCCAAGTCTATTCAA
GTCTTACCCTCACATGAGCTAGTGAGCACCAGGATTGCTGAACATCTGGGTGACAGCCAG
ACCTTGGGTAGAATGCTAGCTGATTATCGAGCTAGAGGAGGAAGAATTCAGTCAAAACAT
TTGGACCCCTTTGTCCATTCAAAAGATGCATCATGTACTTCAGGTGGCAAATCAGGAAAC
AAAACTGAGAGTGATGAAGAGAGGGGATTGATTCCAAGTGATGTAATATGGCCAACAAAG
GTGGACTGTCTGAACCCTGCTAACCACCAGAGGCTCTGTGTGCTCTTCAGCAGCTCTTCT
GCCCAGTCCAGCAATGCTCCCAGTGCTTGTGTCAGTCCTTGGATTGTAACAATGGAGTTT
TATGGAAAGAATGACCTTACACTGGGGATATTTTTAGAAAGATACTGTTTCAGGTCTTCT
TACCAGTGTCCGAGCATGTTCTGTGACACCCCCATGGTTCATCACATTCGACGCTTTGTT
CATGGCCAAGGCTGTGTACAGATAATTCTGAAGGAGTTGGATTCTCCAGTGCCTGGATAT
CAACATACAATTCTCACATATTCCTGGTGCAGAATCTGCAAACAAGTAACACCAGTTGTT
GCTCTTTCAAATGAATCCTGGTCTATGTCATTTGCAAAGTACCTTGAACTTCGATTTTAT
GGCCACCAGTACACACGCAGAGCCAACGCTGAGCCCTGCGGTCACTCTATCCACCATGAT
TATCACCAGTATTTCTCTTATAACCAGATGGTGGCATCTTTCAGTTACTCTCCTATTCGG
CTTCTTGAAGTATGTGTTCCACTACCAAAAATATTCATTAAGCGTCAAGCCCCACTGAAG
GTATCTCTTCTTCAGGACCTCAAAGACTTTTTTCAGAAGGTTTCACAGGTGTACCTAGCT
GTTGATGAGAGACTTGCATCCTTGAAAACGGATACATTTAGCAAAACTAGAGAGGAAAAG
ATGGAAGATATCTTTGCACAAAAGGAGATGGAAGAGGGTGAGTTTAAGAACTGGACAGAG
AAGATGCAAGCAAGGCTCATGTCTTCCTCTGTGGATACCCCTCAGCAACTGCAGTCCATT
TTTGAGTCACTGATTGCCAAGAAGCAAAGCCTCTGTGAGGTGCTCCAGGCGTGGAACAGC
AGGTTGCAGGACCTCTTCCAGCAGGAAAAAGGTAGAAAGAGGCCTTCAGTTCCTCCCAGT
CCTGGGAGACTGAGACAAGGTGAAGAAAGCAAGATAAATGCAATGGACACATCTCCAAGG
AATATTTCTCCAGGACTTCACAATGGAGAAAAAGAGGATCGCTTCTTGACAACCCTGTCC
AGCCAGAGCTCTACGAGCTCCACCCACCTCCAGCTGCCCACTCCTCCCGAGGCCCTGGCC
GAGCAGGTAGTGGGAGGGCCGACTGATCTGGATTCAGCCAGTGGCTCTGAAGATGTATTT
GATGGTCATTTGCTGGGATCCACAGACAGCCAGGTGAAGGAAAAGTCAACCATGAAAGCC
ATCTTTGCTAATTTGCTTCCAGGAAACAGCTACAATCCCATTCCATTTCCTTTTGATCCA
GATAAACACTACTTAATGTATGAACATGAACGGGTGCCCATTGCTGTCTGTGAGAAAGAG
CCCAGCTCCATCATTGCTTTTGCACTCAGTTGTAAAGAATACCGCAATGCCTTAGAGGAA
TTGTCCAAAGCAACTCTGCGGAACAGTGCTGAAGAAGGGCTCCCAGCCAATAGTGCTTTA
GATAACAGACCTAAGAGTAGCAGCCCTATTAGACTACCTGAAATCAGTGGAGGACAGACA
AACCGCACAGTAGAAGCAGAACCTCAGCCAACCAAAAAAGCTTCAGGAATGTTGTCCTTC
TTCAGAGGAACAGCAGGGAAGAGCCCTGATCTGTCTTCCCAGAAGAGGGAGACCTTGCGA
GGGGCAGACAGTGCTTACTACCAGGTTGGGCAGGCCGGCAAGGAGGGGTTGGAGAGTCAA
GGCCTGGAGCCTCAAGATGAAGTAGATGGAGGAGATACACAGAAGAAACAACTCACAAAT
CCTCATGTGGAACTTCAATTTTCTGATGCTAATGCCAAGTTTTACTGTCGGCTGTACTAC
GCGGGAGAGTTCCACAAGATGCGTGAAGTGATTCTGGGCAGCAGTGAGGAGGAATTCATC
CGTTCCCTTTCTCACTCATCTCCCTGGCAGGCCCGGGGAGGCAAGTCAGGAGCTGCTTTC
TATGCCACCGAAGATGATAGATTCATTCTGAAGCAAATGCCTCGTTTGGAAGTCCAGTCT
TTCCTTGACTTTGCACCACACTACTTCAATTATATCACAAATGCTGTTCAACAAAAGAGG
CCCACCGCCTTGGCTAAAATTCTTGGAGTTTACAGAATTGGTTATAAGAACTCTCAGAAC
AACACTGAGAAGAAGTTAGATCTCCTTGTCATGGAAAATCTTTTCTATGGGAGAAAGATG
GCACAGGTTTTTGATTTGAAGGGTTCACTTAGGAATCGAAATGTAAAAACTGACACTGGG
AAAGAGAGCTGTGATGTGGTTCTGTTGGATGAAAACCTCCTAAAGATGGTTCGAGACAAC
CCTCTCTATATTCGTTCCCATTCCAAATCTGTGCTGAGAACCTCCATCCACAGCGACGCC
CATTTCCTTTCCAGCCACCTCATTATAGACTATTCTCTGCTGGTTGGGCGAGATGACACT
AGCAATGAGCTTGTGGTTGGCATCATAGATTACATTCGAACATTTACATGGGACAAAAAA
CTTGAGATGGTTGTGAAGTCAACAGGAATTTTAGGAGGACAAGGTAAAATGCCAACTGTG
GTCTCTCCAGAGTTGTATAGGACTAGATTTTGTGAAGCAATGGACAAGTATTTCTTGATG
GTGCCAGACCACTGGACAGGGTTGGATCTGAATTGC
Protein sequence predicted based on nucleotide sequence
MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERG EGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENTLPHPQESTDS RRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSE KFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSI GEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRL VSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKIL LDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAI GQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKD IKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPL GWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVV QTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKIL LLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLL EHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTL MFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQ SPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKS IPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQ DPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPF LLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQ VLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGN KTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEF YGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGY QHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHD YHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLA VDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSI FESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPR NISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVF DGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKE PSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQT NRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQ GLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFI RSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKR PTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTG KESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDT SNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLM VPDHWTGLDLNC
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
159
End on reference sequence
6317
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
