Sash1 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Sash1
Gene Name:  SAM and SH3 domain containing 1
Original Clone ID:
FLH265561.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080938
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3690nts
Open reading frame: 1 to 3690

ATGGAGGAGGACGCGGGAGCTGCTAGCCCGGCGCCGGAGCCTGAGCCGGAGGTGGATCCC
GCCCGGGAGCTGGAGCCCGAGGCCGGTGTCTCCGAGTCAATCTCCCGCCTCTGGACCGAC
GTGATGGGCATCCTGGATGGTTCCCTGGGAAACATTGATGACCTGGCACAGCAGTATGCA
GATTATTACAATACCTGCTTTTCTGACGTGTGTGAGCGGATGGAGGAGCTCCGGAAACGG
CGCGTTTCCCAGGACCTGGATGTGGAGAAACCCGACGCCAGCCCCACGTCACTTCAGCTG
CGGTCCCAGATCGAAGAGTCTCTAGGCTTCTGCAGCGCCGTGTCAACACCAGAAGTGGAA
AGAAAGTACCCTCTCCATAAATCAAACTCAGAAGACGGCTGTGTAGGCAAAGGAGATTGG
AAGAAGAAAAATAAATACTTCTGGCAGAATTTCCGAAAGAACCAAAAAGGAATAATGAGA
CAGACTTCAAAAGGAGAAGATGTGGGCTATGTAGCCAGCGAGATTACGATGAGTGACGAG
GAGCGGATTCAGCTGATGATGATGGTCAAGGAGAAAATGATCACCATTGAAGAAGCGCTC
GCCAGGCTGAAGGAATACGAGGCCCAGCACCGTCAGTCATCCACCTTGGACCCCGCAGAC
TGGCCCGATGGCTCCTACCCGACATTGGATGGCTCCTCCACCTGCAACTCAAGAGAGCAG
TCGGATGATGAGACAGAGGACTCGGTGAAGTTTAAGAGGTTGCACAAGCTGGTAAACTCT
ACTCGAAGAGTCAGAAAGAAACTAATTAGGGTGGAAGAAATGAAAAAGCCCAGCACAGAA
GGTGGAGAGGAACATGTATTTGAGAATTCCCCAGTCCAAGATGAAAGATCTGCACTCTAC
TCTGGAGTGCACAAAAAGCCATTTTTCTATGATGGCTCTCCTGAGAAACCTCCAGAAGAT
GATGCAGACTCTCTCACCCCATCTCCATCATCCAGCAGCCTGGATACGTGGGGAGCTGGC
CGGAAGTTGGTCAAAACCTTCAGCAAAGGAGAGAGTCGGGGCCTGATTAAGCCCCCTAAG
AAGATGGGGACATTCTTCTCCTATCCAGAAGAAGAGAAGGCCCAGAAAGTGTCCCGCTCC
CTCACAGAGGGGGAAATGAAGAAAGGTCTTGGGTCTTTGAGTCACGGGAGAACCTGCAGT
TTTGGTGGATTCGACCTGACAAACCGTTCTCTGCATGTTGGCAGTAATAATTCTGACCCT
GCGGGTAAAGAGGGAGATTTTGTGTACAAAGAAGTCATCAAATCCCCTCCTGCCCCCCGC
ATCTCTCTCGGGAAAAAGGTGAGATCAGTGAAAGAGACCATGAGGAAGAGGATGTCTAAA
AAGTACAGCAGCCCGGTCTCAGAGCAGGACTCGGGGCTCGATGGAATGCCCAGCTCCCCC
GCCTCAGGAAAGCCTGACTCCGAACACGTGGACAAGCCTAAGCTCAAAGCCGGGGGCTCT
GTGGAAAGCCTTCGGAGTTCTCTGAGTGGCCAGAGCTCAATGAGTGGTCAGACAGTGAGC
ACCACTGATTCCTCCACCAGCAACCGGGAGAGTGTCAAGTCAGAAGATGGGGATGACGAA
GAGCCACCCTATCGGGGTCCCTTCTGTGGGCGTGCCCGAGTGCACACTGACTTCACCCCC
AGTCCCTATGACACGGACTCTCTGAAGCTCAAGAAAGGAGATATCATTGACATAATTAGC
AAACCACCCATGGGTACCTGGATGGGCCTGCTGAACAACAAAGTCGGCACGTTCAAGTTC
ATCTACGTGGATGTGCTAAATGAGGAAGAGGAGAAGCCCAAGCGCCCTACCAGGAGGAGG
AAGAAGGGAAGACCATCTCAGCCGAAGTCTGTGGAGGATCTCCTGGACCGGATCAACCTG
AAAGAACACATGCCCACCTTCCTGTTCAATGGCTATGAAGACCTGGACACCTTCAAACTC
CTGGAGGAAGAAGACTTAGATGAATTAAATATCAGGGACCCAGAACACAGAGCAGTTCTT
CTGACTGCGGTGGAGCTGTTGCAGGAATATGACAGTAACAGTGACCAGTCCGGTTCTCAG
GAAAAGCTGCTGGTGGACAACCAGGGCCTGAGTGGACGATCCCCACGAGACTCCGGATGC
TACGAAAGCAGCGAGAACTTGGAAAACGCCAAAACTCACAAACCCAGCGTTCTGTCTACC
AAGTCGTCCACGGAGTCCAACTTAAAGTCTTTCACCAGGAGTCAGCCAGGCAACTACCCG
ACATTGCCTTTAATGAAATCAGGGGAGGTGCGCAAGCAGGGAGAGGAGGGCAGGCTGGGA
CGAGGTCTAGCCCCGGACACTGCCAAGAGCTGTGACGTGCCCTCTGTGACTGATTTGAGT
AAAAACCGAAGGAGCCTCCCGGTCTCCATCTGTCGCAGCTGTGAAACCCTGGAGGGTCCT
GAGCCTGTGGAAAGTTGGCCCCGATCCCATTCCCTGGATGACCTTCAGGGAGATGCTGAT
GTTGGCAAGAATGTGCCTACCGAGATGCCAGAAACCTGTTCTCAGAATGTACCAGAAGTG
CCGCAGAAGACGTCTGCCTGCACCTCCAAGGCTCTGCCACGAGGCCGAGATCCCACAGCA
GATGTGATGCTTCTGACCCAAAGCAAGAGATTTTCCGACCCCCCGAAAACAATGGCTAAG
AAACTGGATGGCTCCGTGGTGGCCTCCAACCTTGGGATAGCTCCTCCTCAGTGTATACCC
AGAGATTTCGAGGCCCAGCCGCCTGTTAAACCCGGCTTAACAAGGACGTCTCTTGAGGGT
CTCAGAAAAGGACATGATCACCACCCCCTGGGCACCAAAGAAGGGGTAGACGGGGAGCAG
AGCGCCCCTGAGACCAGGACACAGTCCCGACATCCGTCGCAGCCCCCTCCTGTGCCAGCC
AAGAAAAGCCGAGAGCGCCTGGCCAATGGCCTGCACTTGGTCCCCAGCCCAGAGGCACCC
ATCCTGCCACTGAAGAAAGCCAGCCCTGCCAGCCCAGTCAGTCCCAGCGACTGCCCCTCT
CCCCGGGAACCCAGGCCTTCCTCCGGGACTGAGCCGGGCAGCCCAGCCTGTACGAGACCT
CCACCCTGGCTGGCTGAGCTGCCCGAGAGCACTAGCCTGCAGGAGCATGGTGTGAAACTG
GGCCCAGTGCTGAGCAGGAAGGTTTCCTGTGTCCGGGGTGTGGACCTAGAGATGCTCACG
GAGAACAAGCTGCAGGCGGAGGGCATTGATCTCACTGAGGAGCCCTACTCTGATAAGCAT
GGCCGTTGTGGGATCCCGGAAGCCCTGGTACAGAGATACGCAGAGGACCTAGAGCAGCCT
GAGAGGGATGTTGCCACCAACATGGACCAGATCCGGGTGAAGCTGCTTCGGAAGCAACAT
CGGATGGCGATCCCGAGTGGTGGGCTGACGGAAATCTGCAGAAAGCCCCTCTCTCCCGGC
TGTGTTGCGTCCATGTCGGATTGGCTCATCTCTATCGGCCTGCCCATGTACACGAGCACC
CTTTCTGACGCAGGGTTCAGCACGCTGAGCCAAGTGCCTTCCCTGTCCCACTCTTGCCTT
CAGGAGGCCGGCATCACCGAGGAGAGACACATAAGGAAACTCATAACGGCAGCCAGACTC
TTCAAACTGCCACCAAGCCCTGAGGCTATG

Protein sequence predicted based on nucleotide sequence

MEEDAGAASPAPEPEPEVDPARELEPEAGVSESISRLWTDVMGILDGSLGNIDDLAQQYA
DYYNTCFSDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGFCSAVSTPEVE
RKYPLHKSNSEDGCVGKGDWKKKNKYFWQNFRKNQKGIMRQTSKGEDVGYVASEITMSDE
ERIQLMMMVKEKMITIEEALARLKEYEAQHRQSSTLDPADWPDGSYPTLDGSSTCNSREQ
SDDETEDSVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGGEEHVFENSPVQDERSALY
SGVHKKPFFYDGSPEKPPEDDADSLTPSPSSSSLDTWGAGRKLVKTFSKGESRGLIKPPK
KMGTFFSYPEEEKAQKVSRSLTEGEMKKGLGSLSHGRTCSFGGFDLTNRSLHVGSNNSDP
AGKEGDFVYKEVIKSPPAPRISLGKKVRSVKETMRKRMSKKYSSPVSEQDSGLDGMPSSP
ASGKPDSEHVDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDE
EPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKF
IYVDVLNEEEEKPKRPTRRRKKGRPSQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKL
LEEEDLDELNIRDPEHRAVLLTAVELLQEYDSNSDQSGSQEKLLVDNQGLSGRSPRDSGC
YESSENLENAKTHKPSVLSTKSSTESNLKSFTRSQPGNYPTLPLMKSGEVRKQGEEGRLG
RGLAPDTAKSCDVPSVTDLSKNRRSLPVSICRSCETLEGPEPVESWPRSHSLDDLQGDAD
VGKNVPTEMPETCSQNVPEVPQKTSACTSKALPRGRDPTADVMLLTQSKRFSDPPKTMAK
KLDGSVVASNLGIAPPQCIPRDFEAQPPVKPGLTRTSLEGLRKGHDHHPLGTKEGVDGEQ
SAPETRTQSRHPSQPPPVPAKKSRERLANGLHLVPSPEAPILPLKKASPASPVSPSDCPS
PREPRPSSGTEPGSPACTRPPPWLAELPESTSLQEHGVKLGPVLSRKVSCVRGVDLEMLT
ENKLQAEGIDLTEEPYSDKHGRCGIPEALVQRYAEDLEQPERDVATNMDQIRVKLLRKQH
RMAIPSGGLTEICRKPLSPGCVASMSDWLISIGLPMYTSTLSDAGFSTLSQVPSLSHSCL
QEAGITEERHIRKLITAARLFKLPPSPEAM

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 213
End on reference sequence 3905
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable