Gene:
Gene Symbol:
Nlrp9c
Gene Name:
NLR family, pyrin domain containing 9C
Original Clone ID:
FLH265575.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080952
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3012nts
Open reading frame: 1 to 3012
ATGGTTGACTCATCTAGCTATGGCTTGCTGCAGTATTTTCAAAAACTCAGTGATGAAGAG
TTCCAGAGATTTAAAGAGCTTCTCCAGAAAGAGCAAGAGAAATTTAAACTCAAACCACTC
TCCTGGACTAAGATTAAGAACACTTCCAAAGAAGACCTGGTAACGCAATTGTACACACAT
TATCCAAGACAGGTATGGGATATGGTGTTGAACCTGTTTCTACAGGTCAATAGGAAAGAC
CTCTCAACCATGGCTCAGATAGAAAGGAGAGATAAACAAAATAAATACAAAGAGTTTATG
AAGAATTTATTTCAATACATATGGACATCTGAGACCAACACTTATATGCCTGATAGAAGC
TACAACACCATTATAGATCGTCAATACAAAGCATTACTGGACATATTTGATTCTGAGTCA
GACCCAGCTACTGCAGTTGTATTAGGTACTAGAGGAAAAGGAAAAACAGTTTTTTTAAGA
AAAGCAATGTTGGATTGGGCATCAGGAGTTTTACTGCAGAACCGGTTTCAGTATGTTTTC
TTCTTCTCTGTCTTCTCACTTAACAATACCACAGAGTTAAGCTTAGCTGAGCTTATCTCA
AGTAAATTGCCTGAGTGTTCTGAGACACTGGATGACATTTTATCCAATCCAAAGAGAATC
TTGTTTGTTCTGGATGGATTTGATTACCTGAAATTTGACTTGGAACTCAGGACAAACTTG
TGCAATGATTGGAGGAAGAGGCTGCCAACACAAAATGTTTTGAGCAGTTTGCTACAGAAA
ATAATGCTCCCAGAGTGCTCTCTGCTTCTTGAATTGGGAGAGTCAAGTTGTTCAAAAATT
ATTCCTTTGCTGCAGAATCCTAGGGAGATCATTATGTCAGGATTATCTGAACAGTCTATA
TATTTCTACTGTGTGTCCTTCTTCAAAATTCAGTTGGGTGTAGAAGTCTTTAAGGATTTA
AAGAAAAACGAACCATTGTTTACTTTATGCAGCAATCCTAGTATGTTGTGGATGATCTGT
AGTTCTTTAATGTGGGGGCATTATTCGAGAGAGGAAGTAATCTCTTCCAGTGAATCAACA
TCAGCTATCCACACGATATTTATCATGAGTGCATTCAAATCAATATTTGGCTTGGGCTCA
TCTAAATACAAGAGGTTCAAATTAAAGACCCTGTGCACCTTGGCTGTAGAGGGAATGTGG
AAACAGGTATATGTGTTTGACTCTGAGGACCTCAGGAGGAATAAGATATCTGAATCTGAT
AAGACAGTGTGGCTGAAAATGAAATTTCTGCAAATTCAAGGTAACAACATTATGTTTTAC
CATTCTACACTACAGTGGTACTTTGCTACCCTGTTCTATTTTCTCAAACAGGACAAAGAC
ACATATCACCCAGTTATTGGAAGCCTACCTCAACTTTTAGGAGAAATATATGCTCATAAA
CAGAACCAATGGACCCATGCACAGACATTTTTCTTTGGAATTGCCACTAAACATGTGATC
ACTTTGCTTAAACCATGCTTTGGCAATATATCATTCAAAACAATAAGACAGGAAATCATT
AGATACCTTAAAAGTCTCAGTCAACCAGAGTGTAATGAGAAACTGGTGCATCCCAAGAAG
TTGTTCTTCTGTCTAATTGAAAACCAGGAGGAAAGATTTGTAAGTCAAGTGATGAATCTA
TTTGAAGAAATTACTGTTGATATTAGTGATAGTGATGATTTGGGAGCAGCTGAATACAGT
CTGCTGCGAGCCTCAAAATTGAAAAACCTTCACCTGCATATACAAAAGAAAGTTTTTTCA
GAAATCCATGATCCCGAATATGGTAGCTTAGAAAACTTTAAACTGGACCAAAAATTTTCA
GCCATCAATTGGACTATGTTATCCATTCTTTTCTGTAACTTACATGTGTTGGATCTGGGC
AGCTGTCATTTCAATAAAAAGGTCATAGAGGTCCTCTGTAATTCTTTGTCTCCAACTCCT
AATATGCCTCTGACTGTTTTTAAGCTCCAAAGATTGTTGTGTTCCTTCATGACTAACTTT
GGAGATGGTTCATTATTTTGCACATTTCTTCAGATACCACAACTGAAATACCTGAACCTG
TATGGCACTGATCTCTCTAATGATGTGGTTGAGATGCTGTGCTCAGCTCTGAAATGTTCA
ACATGTCGAGTGGAGGAGCTGTTGTTGGGAAAATGTGACATATCAAGTGAGGCTTGTGGC
ATAATGGCAACTTTCCTTATCAACTCTAAGGTGAAACATCTCTCATTGGTTGAAAATCCC
TTGAAGAACAAAGGAGTGATGTTTCTGTGTAAAATGCTGAAGGACCCAAGCTGTGTTCTG
GAATCACTGATGTTGTCTTACTGCTGTCTCACATTCATTGCCTGTGGACATCTCTATGAA
GCTCTTTTAAGCAACAAACACCTCTCTCTCCTTGATCTGGGATCAAATTTCCTGGAAGAT
ATTGGAGTGAACCTTCTGTGTGAAGCTTTGAAATATCCAAACTGCACCCTCAAGGAGTTA
TGGTTGCCTGGTTGCTATTTAACTTCAGAATGCTGTGAAGAGATCTCGGCTGTCCTGACT
TGCAATAAAAATCTAAAGACCCTGAAACTTGGCAACAATAACATACAAGACACTGGTGTC
AAACGGTTATGTGAAGCTCTGTGTCATCCTAAATGTAAAGTGCAGTGTCTTGGGTTAGAC
ATGTGTGAGCTCAGCAATGACTGTTGTGAAGACCTGGCATTGGCTCTTATCACTTGCAAC
ACATTGAAGAGCTTAAATCTGGACTGGAATGCCTTGCATCACTCTGGGCTGGTGATGCTC
TGTGAGGCTCTGAACCACAAAAAGTGTAAACTGAATATGCTTGGACTGGACAAATCTTCA
TTTTCTGAGGAATCACAGACATTTCTGCAAGCTGTGGAAAAGAAAAATAACAATCTGAAT
GTTTTGCATTTTCCCTGGGTTGAAGATGAACTCAAAAAGAGGGGGGTTCGTCTGGTATGG
AACAGCAAGAAT
Protein sequence predicted based on nucleotide sequence
MVDSSSYGLLQYFQKLSDEEFQRFKELLQKEQEKFKLKPLSWTKIKNTSKEDLVTQLYTH YPRQVWDMVLNLFLQVNRKDLSTMAQIERRDKQNKYKEFMKNLFQYIWTSETNTYMPDRS YNTIIDRQYKALLDIFDSESDPATAVVLGTRGKGKTVFLRKAMLDWASGVLLQNRFQYVF FFSVFSLNNTTELSLAELISSKLPECSETLDDILSNPKRILFVLDGFDYLKFDLELRTNL CNDWRKRLPTQNVLSSLLQKIMLPECSLLLELGESSCSKIIPLLQNPREIIMSGLSEQSI YFYCVSFFKIQLGVEVFKDLKKNEPLFTLCSNPSMLWMICSSLMWGHYSREEVISSSEST SAIHTIFIMSAFKSIFGLGSSKYKRFKLKTLCTLAVEGMWKQVYVFDSEDLRRNKISESD KTVWLKMKFLQIQGNNIMFYHSTLQWYFATLFYFLKQDKDTYHPVIGSLPQLLGEIYAHK QNQWTHAQTFFFGIATKHVITLLKPCFGNISFKTIRQEIIRYLKSLSQPECNEKLVHPKK LFFCLIENQEERFVSQVMNLFEEITVDISDSDDLGAAEYSLLRASKLKNLHLHIQKKVFS EIHDPEYGSLENFKLDQKFSAINWTMLSILFCNLHVLDLGSCHFNKKVIEVLCNSLSPTP NMPLTVFKLQRLLCSFMTNFGDGSLFCTFLQIPQLKYLNLYGTDLSNDVVEMLCSALKCS TCRVEELLLGKCDISSEACGIMATFLINSKVKHLSLVENPLKNKGVMFLCKMLKDPSCVL ESLMLSYCCLTFIACGHLYEALLSNKHLSLLDLGSNFLEDIGVNLLCEALKYPNCTLKEL WLPGCYLTSECCEEISAVLTCNKNLKTLKLGNNNIQDTGVKRLCEALCHPKCKVQCLGLD MCELSNDCCEDLALALITCNTLKSLNLDWNALHHSGLVMLCEALNHKKCKLNMLGLDKSS FSEESQTFLQAVEKKNNNLNVLHFPWVEDELKKRGVRLVWNSKN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3020
Start on reference sequence
6
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
