Gene:
Gene Symbol:
Iqsec2
Gene Name:
IQ motif and Sec7 domain 2
Original Clone ID:
FLH265582.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080959
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2847nts
Open reading frame: 1 to 2847
ATGGAACCCCCCTGGAGGAGCAGCAGGTCCACAGCGTCTCATACTCTACACCAGTATTGC
TGTCCTACTCAGGTCCTTGACTCCATGAAGCTTACCCCCTCAGGCAGGCTGGCAGAGAGC
AGTGTGGAGGGTGATGCCCCAGGCAGTGACCTGAGCACAGCGGTTGATAGTCCTGGGAGC
CAACCCCCCTACCGGCTGAGCCAACTACCCCCCACCAGCAGCCACATGGGGGGCCCCCCT
GCCGGGGTGGGCCTTCCTTGGGCTCAGAGGGCACGCCTGCAGCCAGCCAGTGTCGCCCTG
AGGAAGCAGGAGGAGGAGGAGATCAAGCGCTCCAAGGCCCTGTCAGACAGCTATGAACTC
TCCACAGACCTGCAGGACAAGAAGGTGGAAATGCTGGAGAGGAAGTATGGGGGCTCCTTC
CTGAGCCGCAGGGCTGCCAGGACCATCCAGACAGCCTTTCGACAGTACCGTATGAATAAG
AACTTTGAGCGGCTGCGCAGCTCAGCTTCAGAGAGCCGCATGTCCCGCCGCATCATCTTG
TCCAACATGCGGATGCAGTTCTCCTTTGAGGAGTACGAGAAAGCACAGAACCCTGCATAC
TTCGAGGGCAAGCCTGCCTCATTGGATGAGGGTGCCATGGCTGGCGCCCGGAGCCACCGG
CTTGAACGGGGCCTCCCTTATGGAGGCTCCTGTGGTGGGGGCATCGATGGTGGTGGAAGT
TCAGTCACCACATCTGGAGAGTTTTCTAATGACATCACAGAGCTCGAGGACTCCTTCTCC
AAACAGGTAAAGTCTCTGGCTGAATCCATTGATGAGGCCCTGAACTGCCACCCATCAGGG
CCTATGTCTGAGGAGCCAGGGTCAGCCCAACTGGAGAAGCGGGAATCAAAGGAACAGCAA
GAAGACAGCTCAGCCACATCCTTCAGTGACCTCCCCCTGTACTTGGATGACCCAGTTCCT
CCTCCATCACCTGAGCGACTGCCCAGCACAGAGCCCCCACCCCAAGGCCGCCCTGAGTTC
TGGGCACCAGCTCCCCTCCCACCAGTTCCTCCACCAATGCCACCAGGGACCCGGGAAGAT
GGTAGCCGTGAGGAAGGCACTCGAAGGGGTCCTGGGTGCTTGGAGTGCCGGGATTTCCGG
CTCCGGGCTGCACACCTCCCCCTCCTCACCATTGAGCCTCCCAGTGACAGTTCTGTAGAC
CTGAGTGACCGCTCAGATCGCGGCTCTGTCCACCGCCAGCTGGTGTATGAGGCTGATGGC
TGCAGCCCCCATGGGACCCTGAAGCACAAGGGGCCACCAGGCAGGGCCCCAATCCCACAC
CGCCACTACCCTGCCCCTGAGGGTCCAGCTCCAGCCCCACCAGGGCCCCTGCCACCAGCC
CCCAATAGTGGTACTGGGCCCAGTGGTGTGGCTGGAGGTCGGAGGTTGGGGAAGTGTGAG
GCAGCAGGTGAGAACTCTGATGGTGGAGACAATGAGAGTCTGGAGAGCTCCAGCAACTCT
AATGAGACCATCAACTGCAGCTCTGGCTCCTCCTCTCGGGATAGCCTGAGGGAACCGCCA
GCCACTGGCCTGTGCAAGCAGACTTACCAGCGGGAGACAAGGCACAGCTGGGACTCGCCA
GCCTTCAACAATGATGTGGTACAAAGGCGACATTACAGAATTGGCCTCAACCTCTTCAAT
AAGAAGCCAGAGAAGGGTATCCAGTATTTGATCGAACGAGGCTTCCTGTCTGACACTCCT
GTTGGGGTGGCTCACTTCATCCTGGAGAGGAAAGGCCTGAGCCGGCAGATGATAGGGGAA
TTCCTGGGGAATCGGCAGAAGCAGTTCAACAGAGATGTGTTGGACTGTGTGGTGGATGAG
ATGGACTTCTCCTCTATGGATTTGGACGATGCACTCAGGAAGTTCCAATCCCATATCCGA
GTTCAGGGGGAGGCCCAGAAAGTGGAGCGACTCATTGAAGCTTTCAGCCAGCGGTACTGT
GTCTGTAACCCTGCCCTCGTACGCCAGTTCCGGAACCCAGACACCATCTTCATCCTTGCT
TTTGCCATCATCCTCCTCAATACCGATATGTACAGCCCCAGTGTCAAGGCTGAACGCAAG
ATGAAGCTGGATGACTTCATTAAGAACCTGAGAGGAGTTGACAATGGTGAAGACATCCCC
CGCGACCTTCTAGTAGGCATCTACCAGCGCATCCAAGGTCGTGAACTGAGGACCAATGAT
GACCATGTGTCCCAGGTGCAGGCTGTGGAGCGCATGATTGTTGGCAAGAAACCGGTCCTG
TCGCTCCCTCACCGTCGACTGGTTTGCTGCTGCCAGCTCTATGAGGTGCCAGATCCAAAC
CGTCCCCAAAGGCTGGGGCTGCATCAGCGGGAGGTCTTCCTCTTCAATGACCTCCTTGTG
GTCACCAAAATTTTCCAGAAGAAGAAGATCTTGGTGACATACAGCTTCCGTCAGTCCTTT
CCCCTGGTGGAAATGCATATGCAGCTCTTCCAGAATTCATATTATCAGTTTGGGATCAAG
TTACTGTCTGCAGTACCTGGAGGGGAACGAAAAGTCCTCATCATCTTTAATGCTCCTAGC
CTCCAAGACAGGCTACGCTTTACCTCTGACCTGCGGGAGTCCATTGCTGAGGTGCAGGAG
ATGGAGAAGTATCGTGTGGAATCGGAGCTGGAAAAGCAGAAAGGTATGATGCGGCCTAAT
GCCTCACAGCCTGGAGGAGCCAAGGACTCAGTGAATGGGACTCTGGCCCGCAGTAGTCTG
GAGGACACTTATGGGGCAGGCGATGGGCTCAAGCGGGGTGCACTCAGTAGTTCCCTGCGA
GACCTCTCTGATGCAGGGGTCTGTTAT
Protein sequence predicted based on nucleotide sequence
MEPPWRSSRSTASHTLHQYCCPTQVLDSMKLTPSGRLAESSVEGDAPGSDLSTAVDSPGS QPPYRLSQLPPTSSHMGGPPAGVGLPWAQRARLQPASVALRKQEEEEIKRSKALSDSYEL STDLQDKKVEMLERKYGGSFLSRRAARTIQTAFRQYRMNKNFERLRSSASESRMSRRIIL SNMRMQFSFEEYEKAQNPAYFEGKPASLDEGAMAGARSHRLERGLPYGGSCGGGIDGGGS SVTTSGEFSNDITELEDSFSKQVKSLAESIDEALNCHPSGPMSEEPGSAQLEKRESKEQQ EDSSATSFSDLPLYLDDPVPPPSPERLPSTEPPPQGRPEFWAPAPLPPVPPPMPPGTRED GSREEGTRRGPGCLECRDFRLRAAHLPLLTIEPPSDSSVDLSDRSDRGSVHRQLVYEADG CSPHGTLKHKGPPGRAPIPHRHYPAPEGPAPAPPGPLPPAPNSGTGPSGVAGGRRLGKCE AAGENSDGGDNESLESSSNSNETINCSSGSSSRDSLREPPATGLCKQTYQRETRHSWDSP AFNNDVVQRRHYRIGLNLFNKKPEKGIQYLIERGFLSDTPVGVAHFILERKGLSRQMIGE FLGNRQKQFNRDVLDCVVDEMDFSSMDLDDALRKFQSHIRVQGEAQKVERLIEAFSQRYC VCNPALVRQFRNPDTIFILAFAIILLNTDMYSPSVKAERKMKLDDFIKNLRGVDNGEDIP RDLLVGIYQRIQGRELRTNDDHVSQVQAVERMIVGKKPVLSLPHRRLVCCCQLYEVPDPN RPQRLGLHQREVFLFNDLLVVTKIFQKKKILVTYSFRQSFPLVEMHMQLFQNSYYQFGIK LLSAVPGGERKVLIIFNAPSLQDRLRFTSDLRESIAEVQEMEKYRVESELEKQKGMMRPN ASQPGGAKDSVNGTLARSSLEDTYGAGDGLKRGALSSSLRDLSDAGVCY
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2906
Start on reference sequence
57
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
