DNASU Plasmid | Dvl3 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Dvl3

Gene Name: dishevelled 3, dsh homolog (Drosophila)

Original Clone ID:

FLH265591.01F

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00080968

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2148nts

Open reading frame: 1 to 2148

ATGGGCGAGACCAAGATCATCTACCACCTGGACGGCCAGGAGACGCCGTACCTGGTGAAG
CTGCCCCTGCCCGCCGAGCGCGTCACCTTGGCGGACTTTAAGGGCGTTCTGCAGCGGCCC
AGCTATAAGTTCTTCTTCAAGTCTATGGACGACGATTTTGGGGTGGTGAAGGAAGAGATC
TCGGACGACAATGCCAAGCTACCCTGCTTCAATGGCCGGGTGGTGTCCTGGCTGGTATCT
GCCGAGGGCTCACACCCAGAGCCGGCCCCTTTCTGTGCTGACAACCCGTCAGAGCTGCCA
CCTTCGATGGAACGCACAGGAGGCATTGGGGACTCTCGACCCCCCTCCTTCCACCCTCAT
GCTAGTGGGGGCAGCCAGGAGAATCTGGACAATGACACGGAGACCGACTCCTTGGTGTCT
GCCCAACGAGAGCGGCCCCGACGGAGGGATGGCCCGGAGCACGCAGCCCGACTCAATGGA
ACCACAAAGGGTGAGCGGCGACGGGAGCCAGGCGGCTATGACAGTTCATCTACCCTCATG
AGCAGCGAGCTGGAGACTACCAGTTTCTTTGATTCCGATGAGGATGATTCCACCAGCAGG
TTCAGCAGCTCCACAGAACAGAGCAGTGCCTCACGCCTCATGAGAAGACACAAGCGGCGG
CGGCGGAAGCAGAAGGTCTCCCGGATTGAGCGGTCCTCGTCTTTCAGCAGCATCACAGAC
TCCACTATGTCTCTCAACATCATCACCGTCACTCTCAACATGGAAAAGTATAACTTCTTG
GGCATCTCCATTGTGGGCCAAAGCAATGAGCGAGGTGATGGGGGCATCTACATTGGCTCC
ATCATGAAGGGTGGGGCTGTGGCAGCTGATGGACGCATTGAGCCAGGAGATATGCTACTG
CAGGTAAATGAGATCAACTTCGAGAACATGAGCAACGATGACGCAGTCCGAGTCCTTCGG
GAGATTGTGCACAAGCCAGGGCCCATCACTCTGACTGTAGCCAAGTGCTGGGACCCAAGT
CCTCGCGGCTGCTTCACATTACCCCGGAGCGAGCCCATCCGTCCCATTGACCCTGCAGCC
TGGGTCTCTCACACGGCAGCCATGACAGGCACCTTCCCCGCCTATGGCATGAGCCCCTCC
TTAAGCACCATCACCTCTACCAGCTCCTCCATCACCAGCTCCATCCCTGATACAGAGCGC
CTAGATGATTTCCACCTCTCCATCCACAGTGACATGGCGGCCATCGTAAAAGCCATGGCC
TCCCCTGAGTCAGGGTTGGAGGTCCGAGACCGCATGTGGCTCAAGATTACCATTCCCAAT
GCTTTCATCGGCTCGGATGTGGTGGACTGGCTGTACCACAACGTGGAAGGGTTCACCGAC
CGTCGGGAGGCCCGCAAGTATGCTAGCAACCTGCTGAAAGCTGGTTTCATCCGCCATACC
GTCAACAAGATCACGTTCTCCGAGCAGTGCTACTACATCTTTGGCGACCTCTGTGGTAAC
ATGGCCAACCTATCGCTTCACGACCATGATGGCTCCAGTGGCGCCTCTGACCAGGACACA
CTGGCTCCTTTGCCGCACCCAGGAGCTGCCCCTTGGCCTATGGCTTTCCCTTACCAGTAC
CCACCACCTCCGCATCCCTACAACCCACACCCAGGCTTCCCAGAACTGGGCTACAGCTAC
GGTGGGGGCAGCGCCAGCAGTCAGCACAGTGAAGGCAGTCGGAGCAGTGGCTCCAACCGA
AGTGGCAGCGACCGGCGCAAGGAGAAGGACCCAAAAGCTGGGGACTCCAAGTCAGGTGGT
AGCGGCAGCGAGTCAGACCACACTACACGCAGCAGTCTGAGGGGGCCACGGGAGCGGGCA
CCAAGCGAGCGATCAGGCCCAGCGGCCAGCGAGCACAGCCACCGCAGTCACCATTCGCTG
ACCAGCAGCCTGCGCAGCCACCACACACACCCAAGCTATGGCCCACCCGGAGTGCCCCCT
CTCTATGGTCCCCCGATGCTGATGATGACCCCGCCGCCGGCAGCCATGGGTCCCCCAGGA
GCCCCTCCAGGGCGTGACCTGGCCTCGGTGCCCCCGGAACTGACGGCCAGCAGACAGTCC
TTCCGCATGGCAATGGGAAACCCCAGTGAGTTCTTTGTGGATGTGATG

Protein sequence predicted based on nucleotide sequence

MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEI
SDDNAKLPCFNGRVVSWLVSAEGSHPEPAPFCADNPSELPPSMERTGGIGDSRPPSFHPH
ASGGSQENLDNDTETDSLVSAQRERPRRRDGPEHAARLNGTTKGERRREPGGYDSSSTLM
SSELETTSFFDSDEDDSTSRFSSSTEQSSASRLMRRHKRRRRKQKVSRIERSSSFSSITD
STMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLL
QVNEINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPRGCFTLPRSEPIRPIDPAA
WVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDTERLDDFHLSIHSDMAAIVKAMA
SPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYASNLLKAGFIRHT
VNKITFSEQCYYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQY
PPPPHPYNPHPGFPELGYSYGGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGG
SGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLTSSLRSHHTHPSYGPPGVPP
LYGPPMLMMTPPPAAMGPPGAPPGRDLASVPPELTASRQSFRMAMGNPSEFFVDVM

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 132

End on reference sequence 2282

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Dvl3

Symbol Nomenclature :

Dvl3

Designation :

DSH homolog 3|dishevelled-3|segment polarity protein dishevelled homolog DVL-3

Full Nomenclature :

dishevelled 3, dsh homolog (Drosophila)

GENEID :

13544

GI :

151556653

GenBank Accession :

BC148469

Mouse Genome Informatics :

108100

Vega :

OTTMUSG00000035778

Target GenBank:

NM_007889

Protein

SMART domain :

DEP : Domain found in Dishevelled, Egl-10, and Pleckstrin

SMART domain :

DAX : Domain present in Dishevelled and axin

SMART domain :

PDZ : Domain present in PSD-95, Dlg, and ZO-1/2.

Clone

Cloning Information :

265591

HIP Master Clone ID :

197689

IMAGE ID :

100015428

Original Clone ID :

FLH265591.01F

Organism

Species Specific ID:

13544

Genome

Chromosome :

Map Location :

16 A3|16 12.46 cM

Ensembl :

ENSMUSG00000003233

Reagents

Labome :

Dvl3-antibody