A930027K05Rik (M. musculus )
In pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Plch2
Gene Name:  phospholipase C, eta 2
Original Clone ID:
FLH265594.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080971
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3714nts
Open reading frame: 1 to 3714

ATGGGCGGACTGGCCTGGGGACCATCCAGGGCCGCAGGCAGCAGTTGGGTCAATGCCAGT
GGGACCTGGGAACAGCCCTTGCGGGGCTTCAGTGGGCTCCAAGGAGGAAGACGAAGAGGT
AGAGGAGAGAAGGGGATCCCTGAGGAGCCCCTGTGCCAGCTGACACCTCAGCTTGGCCTG
TCGCTGAGGGTCCCTTTTGGCTTGGGGGACTACGGCTTGGATATGCCTGGTCCCCAGCCG
TCTGCTGCCAGCCAGACCACAGGAGCCGTGGCTTGCCTGGCAGAGGTACTCCTCTGGGTT
GGAGGGAGCGTGGTAGTGTCACCAAGATGGCAGCTCAGCCTTGTAGTGGAGCGATGCATG
AGTGCCATGCAAGAGGGGACCCAGATGGTGAAGCTCCGTGGCAGCTCCAAGGGATTGGTC
CGCTTCTACTACCTGGATGAGCACCGCTCCTGCCTCCGATGGCGTCCCTCCCGCAAGAAC
GAGAAAGCCAAGATTTCCATCGACTCCATCCAGGAGGTGAGTGAGGGCCGGCAGTCTGAA
ATCTTCCAGCGCTACCCGGACAGCAGCTTTGACCCCAACTGCTGCTTCAGCATCTACCAT
GGCAGCCACAGAGAGTCGCTGGACTTGGTCTCACCCAGCAGTGAAGAGGCACGTACCTGG
GTCACCGGCCTTCGCTACCTCATGGCTGGCATCAGCGATGAAGACAGCCTAGCCCGTCGC
CAGCGTACCAGGGACCAATGGCTGAAGCAGACGTTTGATGAGGCTGACAAGAACGGGGAC
GGCAGCCTGAGCATCAGTGAGGTTCTGCAGCTGCTACATAAACTCAACGTGAACCTGCCC
CGGCAGAGGGTGAAACAGATGTTCAGGGAGGCGGACACAGATGACCATCAGGGGACATTA
GGCTTTGAGGAATTCTGCGCCTTCTACAAGATGATGTCTACCCGCCGAGACCTCTACCTG
CTCATGCTGACCTACAGCAACCATAAGGACCACTTGGATGCCTCCGACCTGCAGCGCTTT
CTGGAGGTGGAGCAGAAGATGAACGGTGTGACCCTGGAGAGCTGTCAGAACATCATTGAG
CAGTTTGAGCCTTGCCTGGAAAATAAGAGCAAGGGGATGCTGGGGATTGATGGCTTTACC
AACTACACCCGGAGCCCCGCCGGTGACATCTTCAACCCTGAGCACAACAGAGTGCACCAA
GACATGACGCAGCCGCTGAGTCACTACTTCATCACCTCGTCCCACAACACCTACCTCGTG
GGTGACCAGCTCATGTCCCAGTCTCGGGTGGACATGTATGCCTGGGTCCTGCAGGCCGGC
TGCCGCTGTGTGGAGGTGGACTGCTGGGACGGGCCCGATGGAGAACCCATTGTCCATCAT
GGCTACACTCTGACCTCCAAGATCCTTTTCAAAGATGTCATCGAAACCATCAACAAATAC
GCCTTCATCAAGAATGAGTACCCAGTGATCCTGTCCATTGAGAACCACTGTAGTGTTGTT
CAGCAGAAGAAGATGGCCCAGTATCTGACTGACATCCTCGGGGACAAGCTGGACCTCTCC
TCAGTGAGCAGCGAAGATGCCACCATGCTTCCATCTCCACAGATGCTCAAGGGCAAGATC
CTGGTGAAGGGGAAGAAGCTCCCTGCCAATATCAGTGAGGATGCAGAAGAAGGCGAAGTA
TCTGATGAGGACAGTGCTGATGAGATGGAGGATGACTGCAAGCTCCTCAATGGGGATGCC
TCCACCAATCGGAAGCGTGTGGAAAACATTGCAAAGAAGAAGCTGGATTCTCTGATCAAG
GAGTCAAAGATCCGTGACTGTGAAGACCCCAATGACTTCTCTGTGTCCACACTATCCCCC
TCTGGAAAGCTTGGGCGCAAAGCAGAGGCCAAGAAGGGTCAGAGCAAGGTTGAGGAAGAC
GTGGAGGCCGGGGAAGACAGCGGGGTGAGCAGGCAGAATAGCCGCCTCTTCATGAGCAGC
TTCTCCAAGCGCAAGAAAAAAGGCAGCAAGATAAAGAAGGTGGCCAGCGTGGAAGAGGGG
GACGAGACCCTGGACTCCCCAGGAAGCCAGAGCCGAGGGACTGCCCGGCAAAAGAAGACC
ATGAAGCTGTCACGAGCCCTCTCGGACCTAGTGAAATATACCAAGTCTGTGGGGACCCAT
GACGTGGAGATAGAGGTGGTATCTAGCTGGCAGGTGTCGTCCTTCAGTGAGACCAAGGCC
CATCAGATCCTGCAGCAGAAGCCCACCCAGTACCTGCGCTTCAACCAGCACCAGCTCTCG
CGCATATACCCCTCCTCCTACCGTGTGGACTCCAGCAACTACAATCCACAACCCTTCTGG
AACGCTGGTTGCCAGATGGTTGCCCTGAACTACCAGTCAGAGGGGCGGATGCTACAGCTG
AACAGGGCCAAGTTCAGCGCCAACGGTGACTGTGGCTACGTGCTCAAACCCCAGTGCATG
TGCCAGGGTGTCTTCAACCCCAACTCGGAGGATCCCCTGCCGGGGCAGCTCAAGAAGCAG
CTGGCCCTGAGGATCATCAGTGGCCAGCAGCTGCCCAAACCACGGGACTCGGTGCTGGGC
GACCGTGGGGAGATCATCGACCCCTTCGTGGAGGTGGAGGTCATTGGGCTCCCCGTGGAC
TGCAGCAAGGAGCAGACCCGAGTGGTGGACGACAACGGATTCAACCCCATGTGGGAGGAG
ACACTGGTGTTCACCGTGCACATGCCAGAGATTGCGCTTGTACGCTTCCTGGTCTGGGAC
CATGACCCCATTGGACGTGACTTCATCGGCCAGAGGACACTAGCCTTCAGCAGCATAATG
CCAGGCTACCGGCATGTGTACCTAGAAGGGATGGAAGAGGCCTCTATCTTTGTTCATGTG
GCTGTCAGTGACATTAGTGGTAAGGTCAAGCAGACTCTGGGTCTAAAAGGTCTCTTCCTC
CGAGGCACAAAGCCAGGCTCGCTGGACAGTCATGCTGCTGGACAGCCCCTCCCCCGGCCC
TCCGTTAGCCAGAGGCTCCTGCGGCGCACGGCCAGTGCCCCGACCAAAAGCCAGAAGCCA
AGTCGCAAGGGCTTCCCAGAGCTGGCCCTGGGCACACAGGACGCAGGCTCCGAGGGGGCA
GCTGATGACGTGGCACCCTCTAGCCCCAACCCAGCTCTGGAGGCCCCTACTCAAGAGAGG
TCAGGCAGCAGCAGCCCCCGAGGTAAGGCACCAGGAGGAGAAGCAACAGAAGAGAGGACA
CTGGCACAGGTGCGGTCCCCAAATGCCCCCGAAGGCCCTGGACCTGCAGGGATGGCCGCC
ACATGCATGAAGTGTGTGGTGGGCTCCTGCGCCGGTATGGACGTTGAGGGCCTTCAAAGG
GAGCAACAACCCAGCCCTGGGCCTGCAGGCAGCCACATGGCCATCAGCCATCAGCCCAGG
GCCCGGGTAGATTCACTGGGGGGCCCTTGCTGCAGCCCAAGTCCTCGAGCCACCCCAGGG
AGAAGCAAAGAGGCCCCCAAGGGTCCTAGGGCTCGGCGCCAGGGTCCAGGCGGCGGCTCT
GTGTCCTCGGACTCCAGCAGCCCAGACAGCCCAGGCAGCCCCAAGGTGGCCCCCTGCCAG
CCGGAGGGTGCTCACAGGCAGCAGGGGGCGCTGCAGGGAGAGATGAACGCCTTGTTCGTT
CAAAAGCTGGAGGAGATCAGGAGTCATTCCCCCATGTTCTCCACCGTTAGGGAT

Protein sequence predicted based on nucleotide sequence

MGGLAWGPSRAAGSSWVNASGTWEQPLRGFSGLQGGRRRGRGEKGIPEEPLCQLTPQLGL
SLRVPFGLGDYGLDMPGPQPSAASQTTGAVACLAEVLLWVGGSVVVSPRWQLSLVVERCM
SAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSE
IFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARR
QRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTL
GFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIE
QFEPCLENKSKGMLGIDGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLV
GDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKY
AFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKI
LVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIK
ESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSS
FSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTH
DVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFW
NAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQ
LALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEE
TLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHV
AVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKP
SRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERT
LAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPR
ARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQ
PEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTVRD

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3717
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable