DNASU Plasmid | Adrbk2 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Adrbk2

Gene Name: adrenergic receptor kinase, beta 2

Original Clone ID:

FLH265607.01F

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00080984

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2064nts

Open reading frame: 1 to 2064

ATGGCGGACCTGGAGGCCGTGCTGGCCGACGTCAGCTACCTGATGGCCATGGAGAAGAGC
AAGACGGCGCCGGCCGCGCGCGCCAGCAAGAAGGTCGTCCTGCCGGAGCCCAGTATCCGG
AGTGTGATGCAGAGGTATCTGGCAGAGAGAAACGAAATAACCTTTGACAAGATTTTCAAT
CAGAAAATCGGCTTCCTACTATTTAAAGACTTCTGCTTGAATGAAATTGGGGAAGCAGTG
CCTCAGGTGAAATTTTATGAAGAGATAAAAGAATATGAGAAGCTGGACAACGAAGAGGAT
AGGCTACGCCGGAGCCGGCAGATGTACGACGCCTACATCATGAGGGAGCTCCTATCCAGC
ACACACCAATTCTCAAAGCAAGCTGTAGAACATGTCCAGAGCCATCTCTCCAAGAAACAG
GTGACGGCTACACTTTTCCAGCCATACATAGAAGAAATCTGTGAAAGCCTTCGTGGGGAT
ATTTTCCAAAAGTTTATGGAAAGCGATAAATTCACTAGATTCTGTCAGTGGAAGAACGTG
GAGTTGAATATTCACCTGAGCATGAACGACTTCAGTGTGCACAGGATCATCGGCCGAGGA
GGGTTTGGGGAAGTTTATGGCTGCAGGAAAGCAGACACCGGTAAAATGTATGCCATGAAG
TGCTTAGACAAGAAAAGGGTGAAGATGAAGCAGGGGGAGACTCTGGCTTTGAACGAGAGG
ATCATGCTGTCTCTCGTTAGCACTGGGGATTGTCCCTTTATTGTCTGCATGACCTACGCC
TTCCACACGCCGGACAAACTCTGCTTCATCCTGGACCTGATGAACGGGGGCGACATGCAC
TACCATCTCTCTCAACACGGGGTGTTTTCTGAGAAGGAGATGCGGTTTTATGCCAGCGAG
ATCATCCTGGGCCTCGAGCACATGCACACCTGCTTCGTAGTCTACAGAGACCTGAAGCCT
GCGAACATCCTCCTAGATGAATATGGGCACGTGAGGATATCGGATCTCGGCCTTGCCTGC
GATTTCTCCAAAAAGAAGCCTCATGCCAGCGTGGGCACCCATGGGTACATGGCTCCCGAG
GTGTTGCAGAAGGGAACGTGCTATGACAGCAGCGCCGACTGGTTCTCCCTGGGCTGTATG
CTCTTCAAGCTTCTGCGGGGCCACAGCCCCTTCAGGCAGCATAAAACCAAAGACAAGCAT
GAGATAGACCGAATGACCCTGACCGTGAACGTGCAGCTTCCAGATGCCTTCTCCCCTGAG
CTGAGGTCCCTCTTAGAGGGTTTGCTCCAGCGGGACGTGAGCCAGCGGCTGGGCTGCGGA
GGAGGAGGGGCACGAGAGTTGAAGGAGCACATCTTCTTCAAGGGCATTGACTGGCAGCAT
GTGTACTTACGGAAGTACCCGCCACCCCTAATCCCTCCTCGGGGAGAGGTCAACGCTGCA
GATGCCTTCGATATCGGCTCCTTCGATGAGGAAGACACCAAAGGCATTAAGCTGTTGGAC
TGTGACCAGGACCTCTATAAGAACTTCCCACTGGTGATCTCCGAGCGCTGGCAGCAAGAA
GTGGTGGAGACCATCTATGACGCCGTCAATGCTGATACTGATAAAATCGAGGCCAGGAGG
AAGGCTAAAAATAAGCAACTTGGTCAAGAGGAAGATTACGCTATGGGGAAGGACTGCATC
ATGCACGGGTACATGCTGAAGCTGGGGAACCCCTTTCTCACACAGTGGCAAAGACGCTAT
TTTTACCTGTTCCCCAACAGACTGGAGTGGAGAGGAGAGGGCGAGTCTCGGCAAAGTCTA
CTGACCATGGAACAGATCATGTCTGTGGAGGAGACCCAGATTAAAGACAGAAAGTGCATC
TTACTCAGGATAAAGGGAGGGAAGCAATTTGTCTTGCAATGTGAGAGTGACCCCGAGTTT
GCACAGTGGCTGAAGGAGCTGACCTGCACCTTCAATGAGGCCCAGAGACTGCTGCGCCGT
GCCCCCAAATTCCTCAACAAACCACGGGCCGCCATCCTGGAGTTCTCCAAGCCACCACTG
TGTCACAGAAATAGCAGCGGCCTC

Protein sequence predicted based on nucleotide sequence

MADLEAVLADVSYLMAMEKSKTAPAARASKKVVLPEPSIRSVMQRYLAERNEITFDKIFN
QKIGFLLFKDFCLNEIGEAVPQVKFYEEIKEYEKLDNEEDRLRRSRQMYDAYIMRELLSS
THQFSKQAVEHVQSHLSKKQVTATLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNV
ELNIHLSMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRVKMKQGETLALNER
IMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDMHYHLSQHGVFSEKEMRFYASE
IILGLEHMHTCFVVYRDLKPANILLDEYGHVRISDLGLACDFSKKKPHASVGTHGYMAPE
VLQKGTCYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVQLPDAFSPE
LRSLLEGLLQRDVSQRLGCGGGGARELKEHIFFKGIDWQHVYLRKYPPPLIPPRGEVNAA
DAFDIGSFDEEDTKGIKLLDCDQDLYKNFPLVISERWQQEVVETIYDAVNADTDKIEARR
KAKNKQLGQEEDYAMGKDCIMHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQSL
LTMEQIMSVEETQIKDRKCILLRIKGGKQFVLQCESDPEFAQWLKELTCTFNEAQRLLRR
APKFLNKPRAAILEFSKPPLCHRNSSGL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2102

Start on reference sequence 36

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Adrbk2

Symbol Nomenclature :

Adrbk2

SYNONYM :

4833444A01Rik; AI851927; AW551196; Adrbk-2; Bark-2; GRK3

Designation :

beta ARK2|beta-ARK-2|beta-adrenergic receptor kinase 2

Full Nomenclature :

adrenergic receptor kinase, beta 2

GENEID :

320129

GI :

151556633

GenBank Accession :

BC148419

Mouse Genome Informatics :

87941

Target GenBank:

NM_177078

Protein

SMART domain :

PH : Pleckstrin homology domain.

SMART domain :

RGS : Regulator of G protein signalling domain

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

S_TK_X : Extension to Ser/Thr-type protein kinases

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

UniProt :

Q8BX71

UniProt :

Q3UYH7

Clone

Cloning Information :

265607

HIP Master Clone ID :

197705

IMAGE ID :

100015374

Original Clone ID :

FLH265607.01F

Organism

Species Specific ID:

320129

Genome

Chromosome :

Map Location :

5 F|5 55.29 cM

Ensembl :

ENSMUSG00000042249

Reagents

Labome :

Adrbk2-antibody