Gene:
Gene Symbol:
Itgad
Gene Name:
integrin, alpha D
Original Clone ID:
FLH265610.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00080987
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3492nts
Open reading frame: 1 to 3492
ATGGTCCGTGGAGTTGTGATCCTCCTGTGTGGCTGGGCCCTGGCTTCCTGTCATGGGTCT
AACCTGGATGTGGAGAAGCCCGTCGTGTTCAAAGAGGATGCAGCCAGCTTCGGACAGACT
GTGGTGCAGTTTGGTGGATCTCGACTCGTGGTGGGAGCCCCTCTGGAGGCGGTGGCAGTC
AACCAAACAGGACAGTTGTATGACTGTGCGCCTGCCACTGGCGTGTGCCAGCCCATCTTA
CTGCACATTCCCCTAGAGGCAGTGAACATGTCCCTGGGCCTGTCTCTGGTGGCTGACACC
AATAACTCCCAGTTGCTGGCTTGTGGTCCAACTGCACAGAGAGCTTGTGCAAAGAACATG
TATGCAAAAGGTTCCTGCCTCCTTCTGGGCTCCAGCTTGCAGTTCATCCAGGCAATCCCT
GCTACCATGCCAGAGTGTCCAGGACAAGAGATGGACATTGCTTTCCTGATTGATGGCTCC
GGCAGCATTGATCAAAGTGACTTTACCCAGATGAAGGACTTCGTCAAAGCTTTGATGGGC
CAGTTGGCGAGCACCAGCACCTCGTTCTCCCTGATGCAATACTCAAACATCCTGAAGACT
CATTTTACCTTCACGGAATTCAAGAGCAGCCTGAGCCCTCAGAGCCTGGTGGATGCCATC
GTCCAGCTCCAAGGCCTGACGTACACAGCCTCGGGCATCCAGAAAGTGGTGAAAGAGCTA
TTTCATAGCAAGAATGGGGCCCGAAAAAGTGCCAAGAAGATACTAATTGTCATCACAGAT
GGGCAGAAATTCAGAGACCCCCTGGAGTATAGACATGTCATCCCTGAAGCAGAGAAAGCT
GGGATCATTCGCTATGCTATAGGGGTGGGAGATGCCTTCCGGGAACCCACTGCCCTACAG
GAGCTGAACACCATTGGCTCAGCTCCCTCGCAGGACCACGTGTTCAAGGTGGGCAATTTT
GTAGCACTTCGCAGCATCCAGCGGCAAATTCAGGAGAAAATCTTTGCCATTGAAGTTCTC
TATAAGCAAAAGTTTAAAGATGATGGACCAGTTCTGGGGGCTGTGGGAAGCTTCAGCTGG
TCTGGAGGTGCCTTCTTGTACCCCTCAAATATGAGATCCACCTTCATCAACATGTCTCAG
GAGAACGAGGATATGAGGGACGCTTACCTGGGTTACTCCACCGCACTGGCCTTTTGGAAG
GGGGTCCACAGCCTGATCCTGGGGGCCCCTCGCCACCAGCACACGGGGAAGGTTGTCATC
TTTACCCAGGAATCCAGGCACTGGAGGCCCAAGTCTGAAGTCAGAGGGACACAGATCGGC
TCCTACTTTGGGGCATCTCTCTGTTCTGTGGACATGGATAGAGATGGCAGCACTGACCTG
GTCCTGATTGGAGTCCCCCATTACTATGAGCACACCCGAGGGGGGCAGGTGTCGGTGTGC
CCCATGCCTGGTGTGAGGAGCAGGTGGCATTGTGGGACCACCCTCCATGGGGAGCAGGGC
CATCCTTGGGGCCGCTTTGGGGCGGCTCTGACAGTGCTAGGGGACGTGAATGGGGACAGT
CTGGCGGATGTGGCTATTGGTGCACCCGGAGAGGAGGAGAACAGAGGTGCTGTCTACATA
TTTCATGGAGCCTCGAGACAGGACATCGCTCCCTCGCCTAGCCAGCGGGTCACTGGCTCC
CAGCTCTTCCTGAGGCTCCAATATTTTGGGCAGTCATTAAGTGGGGGTCAGGACCTTACA
CAGGATGGCCTGGTGGACCTGGCCGTGGGAGCCCAGGGGCACGTGCTGCTGCTTAGGAGT
CTGCCTTTGCTGAAAGTGGGGATCTCCATTAGATTTGCCCCCTCAGAGGTGGCAAAGACT
GTGTACCAGTGCTGGGGAAGGACTCCCACTGTCCTCGAAGCTGGAGAGGCCACCGTCTGT
CTCACTGTCCGCAAAGGTTCACCTGACCTGTTAGGCGAGTGTTTTCCTAAACGTGATGTC
CAAAGCTCTGTCAGGTATGATCTGGCGTTGGATCCGGGCCGTCTGATTTCTCGTGCCATT
TTTGATGAGACGAAGAACTGCACTTTGACCCGAAGGAAGACTCTGGGGCTTGGTGATCAC
TGCGAAACAATGAAGCTGCTTTTGCCAGACTGTGTGGAGGATGCAGTGACCCCTATCATC
CTGCGCCTTAACTTATCCCTGGCAGGGGACTCTGCTCCATCCAGGAACCTTCGTCCTGTG
CTGGCTGTGGGCTCACAAGACCATGTAACAGCTTCTTTCCCGTTTGAGAAGAACTGTAAG
CAGGAGCTCCTGTGTGAGGGGAACCTGGGCGTCAGCTTCAACTTCTCAGGCCTGCAGGTC
TTGGAGGTAGGAAGCTCCCCAGAGCTCACTGTGACAGTAACAGTTTGGAATGAGGGTGAG
GACAGCTATGGAACCTTAATCAAGTTCTACTACCCAGCAGAGCTATCTTACCGACGGGTG
ACAAGAGCCCAGCAACCTCATCCGTACCCACTACGCCTGGCATGTGAGGCTGAGCCCACG
GGCCAGGAGAGCCTGAGGAGCAGCAGCTGTAGCATCAATCACCCCATCTTCCGAGAAGGT
GCCAAGGCCACCTTCATGATCACATTTGATGTCTCCTACAAGGCCTTCCTGGGAGACAGG
TTGCTTCTGAGGGCCAGCGCAAGCAGTGAGAATAATAAGCCTGAAACCAGCAAGACTGCC
TTCCAGCTGGAGCTTCCGGTGAAGTACACGGTCTATACCGTGATCAGTAGGCAGGAAGAT
TCTACCAAGCATTTCAACTTCTCATCTTCCCACGGGGAGAGACAGAAAGAGGCCGAACAT
CGATATCGTGTGAATAACCTGAGTCCATTGACGCTGGCCATCAGCGTTAACTTCTGGGTC
CCCATCCTTCTGAATGGTGTGGCCGTGTGGGATGTGACTCTGAGGAGCCCAGCACAGAGG
GAACCTCCTCAACATTCCGACCTTCTGACCCAGATCCAAGGACGCTCTGTGCTGGTGAGA
AAAGCCCGTGTGCTGGGGACACGCACGGAATGCACTGATGACTCTGACTGTGTAGACTGC
GCCATCGCCGACTGCCTGCACCTCCGCTGTGACATCCCCTCCTTGGGCACCCTGGATGAG
CTTGACTTCATTCTGAAGGGCAACCTCAGCTTCGGCTGGATCAGTCAGACATTGCAGAAA
AAGGTGTTGCTCCTGAGTGAGGCTGAAATCACATTCAACACATCTGTGTATTCCCAGCTG
CCGGGACAGGAGGCATTTCTGAGAGCCCAGGTGTCAACGATGCTAGAAGAATACGTGGTC
TATGAGCCCGTCTTCCTCATGGTGTTCAGCTCAGTGGGAGGTCTGCTGTTACTGGCTCTC
ATCACTGTGGCGCTGTACAAGCTTGGCTTCTTCAAACGTCAGTATAAAGAGATGCTGGAT
CTACCATCTGCAGATCCTGACCCAGCCGGCCAGGCAGATTCCAACCATGAGACTCCTCCA
CATCTCACGTCC
Protein sequence predicted based on nucleotide sequence
MVRGVVILLCGWALASCHGSNLDVEKPVVFKEDAASFGQTVVQFGGSRLVVGAPLEAVAV NQTGQLYDCAPATGVCQPILLHIPLEAVNMSLGLSLVADTNNSQLLACGPTAQRACAKNM YAKGSCLLLGSSLQFIQAIPATMPECPGQEMDIAFLIDGSGSIDQSDFTQMKDFVKALMG QLASTSTSFSLMQYSNILKTHFTFTEFKSSLSPQSLVDAIVQLQGLTYTASGIQKVVKEL FHSKNGARKSAKKILIVITDGQKFRDPLEYRHVIPEAEKAGIIRYAIGVGDAFREPTALQ ELNTIGSAPSQDHVFKVGNFVALRSIQRQIQEKIFAIEVLYKQKFKDDGPVLGAVGSFSW SGGAFLYPSNMRSTFINMSQENEDMRDAYLGYSTALAFWKGVHSLILGAPRHQHTGKVVI FTQESRHWRPKSEVRGTQIGSYFGASLCSVDMDRDGSTDLVLIGVPHYYEHTRGGQVSVC PMPGVRSRWHCGTTLHGEQGHPWGRFGAALTVLGDVNGDSLADVAIGAPGEEENRGAVYI FHGASRQDIAPSPSQRVTGSQLFLRLQYFGQSLSGGQDLTQDGLVDLAVGAQGHVLLLRS LPLLKVGISIRFAPSEVAKTVYQCWGRTPTVLEAGEATVCLTVRKGSPDLLGECFPKRDV QSSVRYDLALDPGRLISRAIFDETKNCTLTRRKTLGLGDHCETMKLLLPDCVEDAVTPII LRLNLSLAGDSAPSRNLRPVLAVGSQDHVTASFPFEKNCKQELLCEGNLGVSFNFSGLQV LEVGSSPELTVTVTVWNEGEDSYGTLIKFYYPAELSYRRVTRAQQPHPYPLRLACEAEPT GQESLRSSSCSINHPIFREGAKATFMITFDVSYKAFLGDRLLLRASASSENNKPETSKTA FQLELPVKYTVYTVISRQEDSTKHFNFSSSHGERQKEAEHRYRVNNLSPLTLAISVNFWV PILLNGVAVWDVTLRSPAQREPPQHSDLLTQIQGRSVLVRKARVLGTRTECTDDSDCVDC AIADCLHLRCDIPSLGTLDELDFILKGNLSFGWISQTLQKKVLLLSEAEITFNTSVYSQL PGQEAFLRAQVSTMLEEYVVYEPVFLMVFSSVGGLLLLALITVALYKLGFFKRQYKEMLD LPSADPDPAGQADSNHETPPHLTS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
22
End on reference sequence
3516
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
