Gene:
Gene Symbol:
Baz2a
Gene Name:
bromodomain adjacent to zinc finger domain, 2A
Original Clone ID:
FLH265623.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00081000
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5661nts
Open reading frame: 1 to 5661
ATGGAGGCAAACGACCATTTTAACTTTACTGGCCTTCCTCCTGCACCAGCTGCCTCAGGA
CTGAAACCCTCTCCTTCCTCGGGGGAGGGCCTCTACACTAACGGGTCTCCCATGAACTTC
CCCCAGCAAGGGAAAAGTTTGAATGGGGATGTGAATGTTAATGGCTTATCTACTGTATCT
CACACTACTACTTCAGGGATTTTGAACTCTGCTCCCCACTCCTCTAGCACCTCACACCTC
CATCACCCTAACGTGGCCTACGACTGTCTTTGGAACTACTCACAGTACCCATCTGCCAAT
CCTGGCAACAACCTCAAGGACCCACCCCTTCTTTCTCAGTTCCCTGGGGGACAATACCCG
CTCAACGGTATCCTTGGGGGCAACCGACAACCTTCATCCCCAAGTCACAACACTAATCTT
CGAGCTGGGAGCCAAGAGTTCTGGGCCAATGGTACCCAGAGTCCCATGGGGCTTAACTTC
GATTCACAGGAACTGTATGATTCTTTTCCTGATCAGAATTTTGAGGTGATGCCCAATGGA
CCCCCAAGTTTTTTCACCTCCCCTCAGACTTCTCCAATGTTGGGGTCTAGTATCCAGACC
TTTGCACCTTCCCAGGATGTAAGCAGTGACATCCATCCTGATGAAGCAGCAGAAAAGGAG
CTGACTTCAGTTGTGGCAGAAAATGGCACTGGCTTGGTAGGCAGCCTGGAGCTGGAGGAA
GAGCAGCCAGAACTAAAGATGTGTGGCTACAATGGTTCTGTCTCCTCTGTGGAGTCTTTA
CACCAAGAAGTCTCCGTCCTGGTCCCTGATCCCACAGTGAGCTGTCTAGATGATCCTTCA
CATCTTCCTGATCAACTGGAAGACACTCCAATTCTCAGTGAAGACTCCCTGGAGCCCTTT
GACTCTCTGGCAGCAGAGCCAGTGAGTGGCAGTCTTTATGGTATAGATGATGCGGAGCTG
ATGGGTGCAGAAGACAAGTTGCCTCTGGAGGGCAACCCTGTGATCTCTGCCCTCGATTGC
CCTGCTCTCAGTAATGCTAATGCCTTCAGTCTCCTGGCAGACGACAGCCAGACATCAGCC
TCCATCTTTGTCAGCCCTACCTCCCCACCTGTCTTAGGGGAGTCTGTCTTGCAAGATAAC
AGCTTTGGACTGAACAGTTGCAGTGACTCTGAACAGGAAGAAATAGAGACCCAGTCTTCA
AACTTCCAACGTCCCCTGACTGAGCCAGCTCCTGACCAGCCACCTAGTACTCAACTACAT
CCAGCAGTTTCACCAACAGCCTCCCCAGCAGCCTCCTTGACAGCATCTGCAGAAATCTCT
CCAGCTGTCTCTCCAGTAGCATCCTCGCCTGTCCCTCCTGAAGTCTTTGTAGCAGTCTCT
CCAGCTTCCTCACCTGCTCTGCCAGCCATCTCTTTGGAAGCCTCTATGACAACTCCAGTA
ACTTCTCCTCAAGGTTCCCCTGAACCTTCTCCAGCAGCTGCCTTCCAGACTGTCTCCCCA
GCAAGGAAAAATGTCAGCAGTGCTCCTAAAGCGCGTGCTGATCGAGAAGAGACGACTGGA
GGAGCAGTTGCAGTCTCTGGTAGTGGTGATGTACTGAAGAGACGTATTGCTACCCCAGAA
GAAGTTCGTCTTCCCCTCCAGCATGGGTGGCGAAGAGAAGTGCGCATCAAGAAGGGCAGC
CATCGGTGGCAGGGGGAGACTTGGTACTATGGCCCCTGTGGGAAGAGAATGAAGCAATTT
CCAGAAGTTATCAAGTACCTGAGCCGAAATGTGGTGCACAGTGTCCGCCGTGAGCACTTC
AGCTTCAGTCCCCGCATGCCTGTTGGAGATTTCTTTGAAGAAAGAGATACACCAGAGGGC
TTGCAGTGGGTCCAGTTATCAGCAGAGGAGATTCCTTCCAGAATTCAAGCAATCACTGGC
AAACGAGGCCGACCTCGAAACAATGAGAAGGCTAAGAACAAGGAAGTTCCCAAAGTGAAG
CGGGGCCGAGGTCGGCCTCCTAAGATCAAAATGCCTGAGCTGTTGAATAAAACAGATAAC
CGACTTCCAAAGAAACTGGAAACCCAAGAAATACTGAGTGAGGACGATAAAGCAAAGATG
ACTAAAAACAAAAAGAAGATGAGGCAGAAGGTCCAACGGGGAGAAAGTCAGACTCCTGTC
CAAGGGCAGGCCAGAAACAAGAGGAAGCAAGACACCAAGAGCTTGAAGCAGAAGGACACT
AAGAAGAAATTGAAGGCTGAGAAAGAGAAGATGAAGACAAAGCAGGAAAAGCTGAAGGAA
AAGGTAAAGCGAGAAAAGAAAGAAAAGGTAAAAGCGAAGGGGAAGGAAGGGCCCAGAGCC
AGGCCATCCTGTAGAGCAGACAAGACCCTTGCCACACAGAAGCGGCTAGAGGAGCAGCAG
AGGCAGCAGGCTATCCTGGAGGAGATGAAGAAGCCCACAGAGGATATGTGTCTGTCTGAC
CACCAGCCCCTGCCTGACTTCACACGCATCCCTGGTTTGACACTGTCCAGTAGGGCTTTC
TCAGATTGCTTGACCATCGTGGAGTTCCTTCACAGTTTTGGCAAAGTGCTAGGCTTTGAC
CTTACCAAAGATGTTCCTAGTCTAGGAGTCCTGCAGGAGGGACTCTTATGTCAAGGTGAC
AGCTTGGACAAAGTGCAGGACCTGCTGGTGCGACTGCTGAAGGCTGCACTCCATGATCCT
GGTCTGCCCCCCTACTGTCAGTCCCTGAAGATATTGGGGGAGAAGATGTCAGAGATCCCA
TTGACCAGAGATAATGTGTCTGAGATACTGCGCTGCTTCCTCATGGCATATAGAGTGGAG
CCATCCTTCTGTGACAGTCTGCGTACCCAGCCTTTTCAGGCCCAGCCACCTCAACAGAAG
GCTGCTATTCTAGCCTTCCTTGTGCATGAGCTTAACAGCTCCACCATTATCATCAATGAG
ATTGACAAGACTCTGGAAAGTGTGTCTAGCTGCAGGAAGAACAAGTGGATTGTTGAAGGC
CGACTCCGGAGACTGAAAACTGCTCTGGCCAAGCGAACTGGGCGGCCTGAGGTTATGATG
GAAGGGGCAGAAGACGGCCTAGGACGGAGGCGCAGTTCTCGGATCATGGAGGAAACCAGT
GGCATAGAAGAGGAGGAAGAGGAAGAAAATACAACAGCTGTCCATGGCCGCAGGGGTCGA
AAAGAAGGAGAGATTGATGTTGCAGCATCTAGCATTCCAGAGCTAGAGCGCCATATAGAA
AAACTCAGTAAGCGTCAGCTCTTCTTTAGAAAAAAGCTGCTTCACTCATCCCAGATGCTT
CGGGCAGTGTCCTTGGGTCAAGACCGCTATAGACGCCATTACTGGGTATTACCGTATCTT
GCTGGTATCTTTGTGGAAGGATCGGAAGGGAGCACAGTTACTGAAGATGAAATAAAGCAA
GAAACTGAGTCCTTGATGGAAGTAGTCACTTCAACACCCAGCTCTGCCCGAGCCTCTGTA
AAGAGAGAATTAACTGGCTCCAATGCCTCTACTTCTCCTGCCCGGTCCCGAGGCCGACCT
CGAAAACCTAAGCCTGGGTCTCTGCAGCCTCAGCACCTTCAGTCCACCATTAGGGAATGT
GATTCAGAGCAAGCCCAGACTCAAGTTCACCCAGAACCCCAGCCTCAGCTTCAGGCCCCT
ACCCAGCCCCATCTTCAGCCAAGTAGTGGGTTCCTAGAGCCAGAAGGTTCCCCTTTCTCT
CTGGGTCAGAGCCAGCATGACCTCAGCCAGTCTGCCTTCTTGTCTTGGCTGAGCCAGACT
CAGAGCCACAACTCCCTGTTGAGCAGCTCAGTCCTCACGCCGGACAGCAGCCCAGGGAAA
CTAGACTCTGCTCCGTCTCAGTCCTTGGAGGAGCCCGAGCCTGATGAGGCTCAGTCCTGC
CCTGGTCCTCAAGGTCCCTGGTTTAACTTCTCAGCCCAGATACCCTGTGATGCTGCTCCT
ACACCACCTCCTGCAGTCTCTGAGGACCAACCTACTCCCTCCCTCCAGCTGCTGGCCTCC
TCTAAACCAATGAATACACCCGGTGCTGCCAATCCTTGTTCCCCAGTGCAGCTCTCTTCC
ACTCACTTGCCTGGAGGGACCCCTAAGAGGCTATCAGGGGACTCTGAAGAAATGTCACAG
AGTCCCACTGGGCTGGGGCAACCAAAGCGGAGGGGGAGACCCCCTAGCAAGTTCTTCAAG
CAGGTGGAGCAGCATTACTTAACCCAGCTGACAGCCCAGCCTATCCCCCCTGAGATGTGC
TCGGGCTGGTGGTGGATCCGAGACCCTGAGACACTGGATGTCCTGCTCAAGGCACTGCAT
CCCCGAGGCATCCGGGAGAAGGCGCTTCACAAACATCTTAGCAAGCACAAGGACTTTTTG
CAGGAAGTTTGTTTACAGCCCTTAACTGATCCCATCTTTGAGCCTAATGAGCTCCCTGCC
TTGGAAGAAGGCGTTATGAGCTGGTCCCCCAAAGAGAAGACGTACGAGACAGACCTAGCT
GTGCTCCAGTGGGTGGAGGAGCTGGAGCAGCGGGTTGTCCTCTCCGATCTGCAGATTCGG
GGCTGGACATGCCCTACCCCAGACTCCACCAGAGAAGACTTGACCTACTGTGAGCATCTG
CCTGACTCCCCGGAGGATATCCCTTGGAGGGGTCGGGGCAGGGAAGGAACAGTACCTCAG
CGGCAGAACAACAACCCTCTGGACCTCGCTGTGATGCGATTGGCTGTTCTGGAGCAAAAT
GTGGAGCGGCGGTACTTGCGGGAGCCCCTCTGGGCAGCCCATGAGGTGGTAGTGGAGAAG
GCCCTACTGAGCACACCCAATGGTGCCCCTGATGGCACCTCAACTGAGATATCCTATGAG
ATCACCCCTCGTGTCCGAGTTTGGCGGCAGACACTTGAAAGGTGCCGTAGTGCAGCCCAA
GTGTGCTTGTGCATGGGCCAGCTAGAAAGGTCCATCGCGTGGGAGAAGTCTGTCAACAAA
GTGACCTGCCTGGTCTGCCGGAAGGGCGATAATGATGAGTTTCTCCTGCTGTGTGATGGG
TGTGACCGAGGCTGCCACATTTACTGTCATCGGCCCAAGATGGAGGCTGTTCCAGAAGGA
GATTGGTTCTGTGCTGTCTGTCTGTCCCAGCAGGTAGAGGAAGAGTACACTCAGAGGCCT
GGTTTTCCAAAACGAGGTCAGAAGCGGAAAAGTAGTTTTCCACTGACCTTCCCAGAAGGT
GACAGCCGGCGGCGGATGTTGTCAAGGAGCCGAGATAGTCCAGCAGTGCCTCGGTACCCA
GAAGACGGGCTGTCTCCCCCCAAAAGACGGCGACATTCGATGAGAAGTCACCACAGTGAT
CTCACATTTTGCGAGATTATCCTGATGGAGATGGAGTCCCATGATGCAGCCTGGCCTTTC
CTAGAGCCTGTGAACCCTCGCTTGGTGAGTGGATACCGACGTGTCATCAAGAACCCTATG
GATTTTTCCACCATGCGAGAACGCCTGCTCCGTGGAGGGTACACTAGCTCAGAAGAGTTT
GCAGCTGATGCTCTGCTGGTTTTTGACAACTGCCAGACCTTCAATGAGGATGACTCTGAA
GTGGGCAAGGCTGGGCACGTCATGCGACGCTTCTTTGAGAGCCGCTGGGAGGAATTTTAT
CAGGGAAAACAGGCCAATCTG
Protein sequence predicted based on nucleotide sequence
MEANDHFNFTGLPPAPAASGLKPSPSSGEGLYTNGSPMNFPQQGKSLNGDVNVNGLSTVS HTTTSGILNSAPHSSSTSHLHHPNVAYDCLWNYSQYPSANPGNNLKDPPLLSQFPGGQYP LNGILGGNRQPSSPSHNTNLRAGSQEFWANGTQSPMGLNFDSQELYDSFPDQNFEVMPNG PPSFFTSPQTSPMLGSSIQTFAPSQDVSSDIHPDEAAEKELTSVVAENGTGLVGSLELEE EQPELKMCGYNGSVSSVESLHQEVSVLVPDPTVSCLDDPSHLPDQLEDTPILSEDSLEPF DSLAAEPVSGSLYGIDDAELMGAEDKLPLEGNPVISALDCPALSNANAFSLLADDSQTSA SIFVSPTSPPVLGESVLQDNSFGLNSCSDSEQEEIETQSSNFQRPLTEPAPDQPPSTQLH PAVSPTASPAASLTASAEISPAVSPVASSPVPPEVFVAVSPASSPALPAISLEASMTTPV TSPQGSPEPSPAAAFQTVSPARKNVSSAPKARADREETTGGAVAVSGSGDVLKRRIATPE EVRLPLQHGWRREVRIKKGSHRWQGETWYYGPCGKRMKQFPEVIKYLSRNVVHSVRREHF SFSPRMPVGDFFEERDTPEGLQWVQLSAEEIPSRIQAITGKRGRPRNNEKAKNKEVPKVK RGRGRPPKIKMPELLNKTDNRLPKKLETQEILSEDDKAKMTKNKKKMRQKVQRGESQTPV QGQARNKRKQDTKSLKQKDTKKKLKAEKEKMKTKQEKLKEKVKREKKEKVKAKGKEGPRA RPSCRADKTLATQKRLEEQQRQQAILEEMKKPTEDMCLSDHQPLPDFTRIPGLTLSSRAF SDCLTIVEFLHSFGKVLGFDLTKDVPSLGVLQEGLLCQGDSLDKVQDLLVRLLKAALHDP GLPPYCQSLKILGEKMSEIPLTRDNVSEILRCFLMAYRVEPSFCDSLRTQPFQAQPPQQK AAILAFLVHELNSSTIIINEIDKTLESVSSCRKNKWIVEGRLRRLKTALAKRTGRPEVMM EGAEDGLGRRRSSRIMEETSGIEEEEEEENTTAVHGRRGRKEGEIDVAASSIPELERHIE KLSKRQLFFRKKLLHSSQMLRAVSLGQDRYRRHYWVLPYLAGIFVEGSEGSTVTEDEIKQ ETESLMEVVTSTPSSARASVKRELTGSNASTSPARSRGRPRKPKPGSLQPQHLQSTIREC DSEQAQTQVHPEPQPQLQAPTQPHLQPSSGFLEPEGSPFSLGQSQHDLSQSAFLSWLSQT QSHNSLLSSSVLTPDSSPGKLDSAPSQSLEEPEPDEAQSCPGPQGPWFNFSAQIPCDAAP TPPPAVSEDQPTPSLQLLASSKPMNTPGAANPCSPVQLSSTHLPGGTPKRLSGDSEEMSQ SPTGLGQPKRRGRPPSKFFKQVEQHYLTQLTAQPIPPEMCSGWWWIRDPETLDVLLKALH PRGIREKALHKHLSKHKDFLQEVCLQPLTDPIFEPNELPALEEGVMSWSPKEKTYETDLA VLQWVEELEQRVVLSDLQIRGWTCPTPDSTREDLTYCEHLPDSPEDIPWRGRGREGTVPQ RQNNNPLDLAVMRLAVLEQNVERRYLREPLWAAHEVVVEKALLSTPNGAPDGTSTEISYE ITPRVRVWRQTLERCRSAAQVCLCMGQLERSIAWEKSVNKVTCLVCRKGDNDEFLLLCDG CDRGCHIYCHRPKMEAVPEGDWFCAVCLSQQVEEEYTQRPGFPKRGQKRKSSFPLTFPEG DSRRRMLSRSRDSPAVPRYPEDGLSPPKRRRHSMRSHHSDLTFCEIILMEMESHDAAWPF LEPVNPRLVSGYRRVIKNPMDFSTMRERLLRGGYTSSEEFAADALLVFDNCQTFNEDDSE VGKAGHVMRRFFESRWEEFYQGKQANL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
156
End on reference sequence
5819
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
