Gene:
Gene Symbol:
Osbpl1a
Gene Name:
oxysterol binding protein-like 1A
Original Clone ID:
FLH265625.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00081002
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2850nts
Open reading frame: 1 to 2850
ATGAACACAGAAGCAGAACAGCAGCTTCTCCATCATGCCAGAAATGGCAATGCTGAAGAG
GTGCGAAAGCTGCTGGCAGCCATGGCACGGATGGAAGTGGTCGCTGACATCGACTGTAAA
GGAAGAAGTAAGTCTAACTTGGGCTGGACACCTCTTCACCTGGCATGCTATTTTGGACAC
AAACAAGTGGTCGAGGATCTGTTGAAGGCTGGTGCAAAAGTGAACATGTTAAACGACATG
GGGGACACGCCACTGCATCGAGCCGCCTTCACAGGACGAAAGGAGCTGGTCTTGCTTCTT
TTAGAATACGATGCTGACAGTACAGTTGTAAATGGAAGTGGACAGACAGCAAAAGAAGCC
ACCCATGACAAAGAAATCAGAAACATGCTTGAAGCTGTGGAGAGGACTCAACAAAGAAAG
CTTGAAGAATTGCTTTTAGGAGCAGCTAGAGAAGGAAGAACAGCCGAAGTATCAGCTCTG
CTCAGCAGGCCCAATCCTCCTGATGTGAACTGCTCAGATCAGCTAGGAAATACCCCCTTA
CACTGTGCCGCCTACCGGGCCCACAAGCAGTGTGTCCTAAAGCTCCTGAGAAGCGGAGCT
GACCCCAGCCTGAAGAACAAGAACGATCAGAAACCTCTTGACCTTGCCCAGGGTGCTGAA
ATGAAGCATATTCTTGTTGGTAATAAGGTTGTCCACAAAGCACTGAAACGCTACGAAGGC
CCTCTCTGGAAGAGTTCAAGATTTTTTGGCTGGAAGTTATTTTGGGTAGTGTTAGAACAT
GGAGTCCTTTCCTGGTATAGGAAGCAGCCTGATGCAGTCCATAATTCTTATCGCCAAGGA
TGCAAACACCTGACTCAAGCCGTGTGCACGGTGAAACCTACAGATAGCTGCCTCTTCTCC
ATTCGATGCTTTGATGACACTGTTCACTGCTTCAGGGTTCCTAAGAACAGCGTACAGCAA
TCAAGAGAGAAGTGGCTGGAAGCAATTGAAGAACACTCCGCATACAGCACTCACTACTGT
TCCCAGGATCAGGTGACTGATGATGAAGAAGAAGATGTAGTTTCGGCCATGGACTTGAAG
GAGTCGTTAGCGAGAGCACAAACATGTCAACAGAGACTAGATAGGGAAATTTACAACTTT
CTCAAAATGATTAAGGAGTGTGATGTGGCCAAAGATATGCTTCCATCGTTTCTTCAGAAA
GCTGATATTGTCTCCGAAGCTTCTAGAGAGACTTGTGTGGCGTTGAATGACTGTCTTAAT
CTCTTCACTAAACAGGAAGGGGTGAGGAATTTTAAATTGGAACAGGAGCAAGAAAAAAAC
AAAATTTTGTCAGAAGCACTGGAGACTTTAGCCACTGAGCATCATGAACTAGAACGATCC
CTGGTTGAAGGATCCCCGCCTGTCAGCATCCTTAGTGAGGAGGAGTTCTATGATGCACTG
TCAGGTTCCGAGTCCGAGGGGTCGCTGACTTGCCTGGAAGCTGTGACAGCACACTCCTTT
GAAGAGAACGAAGTGCCTGGGAGCAGCGGAAAGCACAGAATGTCTGAAGGAAAAGACTGT
GGTGGGGGAGATGCGCTCTCCAATGGCATCAAAAAGCACAGAACAAGCCTGCCATCACCT
ATGTTCTCCAGAAACGATTTCAGCATCTGGAGCATCCTCAGAAAATGCATTGGGATGGAA
CTGTCCAAGATCACAATGCCAGTGATATTTAATGAGCCTCTGAGCTTCCTGCAGCGGCTA
ACTGAATACATGGAGCACACGTACCTCATCCACAAAGCCAGTTCACTTTCTGATCCTGTG
GAAAGGATGCAGTGTGTGGCTGCATTTGCTGTGTCTGCTGTCGCCTCTCAGTGGGAGCGC
ACCGGAAAGCCCTTCAACCCGCTTCTGGGAGAGACTTATGAATTAGTTCGAGATGACCTT
GGGTTTAGGCTCATCTCAGAACAGGTCAGCCATCATCCCCCAATCAGTGCATTCCATGCA
GAAGGGCTCAACAATGACTTCATCTTCCATGGCTCAATTTACCCCAAACTGAAGTTCTGG
GGCAAGAGTGTAGAAGCAGAGCCTAAAGGAACCATCACCTTGGAGCTTTTAGACCACAAC
GAAGCATACACATGGACAAACCCCACCTGTTGTGTGCATAACATCATCGTGGGCAAGCTC
TGGATTGAACAGTATGGCAACGTGGAAATCATAAACCACAAGACTGGGGACAAATGTGTG
CTGAATTTTAAGCCATGTGGTCTTTTTGGCAAGGAATTACACAAAGTTGAAGGCTACATA
CAAGATAAAAGCAAAAAGAAGCTCTGTGCTCTCTATGGGAAGTGGACTGAGTGCTTGTAC
AGCGTAGACCCTGCCACTTTTGATGCTTACAAAAAAAATGATAAGAAAAACACAGAAGAG
AAGAAGAACAGCAAACAGACAAGCTCATCTGAGGAGTCTGATGAAATGCCAGTGCCAGAT
TCCGAGAGCGTATTCATTATCCCTGGAAGTGTGCTCCTGTGGCGGATAGCCCCGCGGCCT
CCCAACTCTGCTCAGATGTATAACTTTACCAGCTTTGCCATGGTTTTGAATGAAGTCGAC
AAGGAGATGGAGAGTGTGATTCCCAAGACTGACTGCAGGCTACGGCCTGACATCAGAGCC
ATGGAGAACGGAGAGATAGATCTAGCTAGTGAAGAAAAGAAACGGCTTGAGGAAAAGCAA
AGAGCAGCCCGCAAGAACAGGTCCAAGTCGGAAGAGGACTGGAAGACAAGGTGGTTCCAT
CAAGGTCCTAACCCCTACAGTGGAGCACAGGACTGGATTTATTCTGGCAGCTACTGGGAC
AGAAACTACTTCAATTTGCCTGATATTTAT
Protein sequence predicted based on nucleotide sequence
MNTEAEQQLLHHARNGNAEEVRKLLAAMARMEVVADIDCKGRSKSNLGWTPLHLACYFGH KQVVEDLLKAGAKVNMLNDMGDTPLHRAAFTGRKELVLLLLEYDADSTVVNGSGQTAKEA THDKEIRNMLEAVERTQQRKLEELLLGAAREGRTAEVSALLSRPNPPDVNCSDQLGNTPL HCAAYRAHKQCVLKLLRSGADPSLKNKNDQKPLDLAQGAEMKHILVGNKVVHKALKRYEG PLWKSSRFFGWKLFWVVLEHGVLSWYRKQPDAVHNSYRQGCKHLTQAVCTVKPTDSCLFS IRCFDDTVHCFRVPKNSVQQSREKWLEAIEEHSAYSTHYCSQDQVTDDEEEDVVSAMDLK ESLARAQTCQQRLDREIYNFLKMIKECDVAKDMLPSFLQKADIVSEASRETCVALNDCLN LFTKQEGVRNFKLEQEQEKNKILSEALETLATEHHELERSLVEGSPPVSILSEEEFYDAL SGSESEGSLTCLEAVTAHSFEENEVPGSSGKHRMSEGKDCGGGDALSNGIKKHRTSLPSP MFSRNDFSIWSILRKCIGMELSKITMPVIFNEPLSFLQRLTEYMEHTYLIHKASSLSDPV ERMQCVAAFAVSAVASQWERTGKPFNPLLGETYELVRDDLGFRLISEQVSHHPPISAFHA EGLNNDFIFHGSIYPKLKFWGKSVEAEPKGTITLELLDHNEAYTWTNPTCCVHNIIVGKL WIEQYGNVEIINHKTGDKCVLNFKPCGLFGKELHKVEGYIQDKSKKKLCALYGKWTECLY SVDPATFDAYKKNDKKNTEEKKNSKQTSSSEESDEMPVPDSESVFIIPGSVLLWRIAPRP PNSAQMYNFTSFAMVLNEVDKEMESVIPKTDCRLRPDIRAMENGEIDLASEEKKRLEEKQ RAARKNRSKSEEDWKTRWFHQGPNPYSGAQDWIYSGSYWDRNYFNLPDIY
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2944
Start on reference sequence
92
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
