Gene:
Gene Symbol:
Gpr113
Gene Name:
G protein-coupled receptor 113
Original Clone ID:
FLH265664.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00081040
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2976nts
Open reading frame: 1 to 2976
ATGTCCTCTCTAGCTCTCTCCCAGCTGCTCCTGGCCGTGACTCTTCCCCTGCTGGAGTTG
GAGCCAACATTTGTCCCCACAGCCCAATCAGAGCTCAGTCCATATGGAGGGAAATCTGGG
CAGCAACTGAACCAGTACAGTGGAGAAGGGGAATCAGTGCTGGTCTCCAGCTATGTGCAT
CTGGAATTTTCAAGCACAGCCTGGCCACAGGAGCTCTCTAAAAACTTTACTCTTCCTACC
GCCCTGGCAGTCCTTCCCCCGAAAACGCTTACTGGCCTTGGCCTTACAACAGAGTGTCGT
GCTAACCACAGCGGAACCAGCCTCTGCACTTGCCACCCTGGCTACCAATGGAATGCCACA
CTTTGTTCCCTTTACCCACACTGCTGGGGCCGCAGGTCAGAAAGAGATTCCTGTATGTGT
CGCAGCTTCCACGGTCCTGTTACTGGCTACTGCCAGTTGCTGCCACCTGTTCCTGCAAAC
CTGATACTGGACTCCCAGCTGCAAATGCACGGCAACACCCTGAACCTCATTCTCCTCAAG
AAAGAGGAAGCCACAAACCTGAGATGGTTCTTGAGACACTCAAAGAACCACACACCCATC
AGCCTATGGCCAGGGACCAATGTGTTGCAGACGTCCAGTGAGGGCCAGTCTGGCCTTCGT
GTCGCCCGCATGTCCCGTCATTGGGCAGGTGAGTACGAGAGCATCTTCGAAGCCCAGGGG
TTCAGGTGGAGGCTGCGGCAGCTGGTGAAGGTGCCCTTGCAGGAGGAAGAAGTCGTTCGA
CTCCCAGACGCCCTGTCCATCTCCTGTACTGCCTCCACTGGCTTTCAGCTGAGCTGCTGC
ATCCCACTTACAACAGACTACACTGCTACCTGGAGTCCTGGGGAGGACAGCCAAGCCTCC
TTGCAAAAAATGTCGGATTCCCAATGCTTTGTGCTAGCTGTTCCGCACTGCCCAGCAACC
AATACCACATACACCTGTACCCTTCAGAGTCAGAACCTGCCTCCTCTCGTGGCTCCTGTC
TCTATCACGATTATCCAGGATGGAGACACTACCTGCCCTGAGGACTTCTCAGTGGTTAGC
TGGAATGTCACCAAGGCTGGCTTCGTGGCACAGGCCCCATGTCCTGTGAATAAGAAAGGT
GTGGTAAAGAGGCTCTGTGGGACTGATGGCATCTGGAGGCCAGTCCAGAACACCTGCACA
GAAGCAAAGATCCTAACCTTGTGTCTTAAAGCCAAGCTGCTGGAAGGCCAGGGAAAGCCC
TACGAGGAAGTGCCATGGATCTTGAGTGAGCTGCAAGAGCAGGTGGGCGTGGCCAGCACC
CCCTCTGACTTATGGGAAATGCTGCACACTGTGACCTTACTGGCCAAGGTGGTAGCAGAG
ACAAGCACAGAGCTCACAGGCAGTGCTCTGAAGGACCTCCTGACGACCACAGACAAGATT
CTAGACGCGAACATCAGCGCTCTGTGGACTCTGGCCCAGGCCCAGGAGCCCTCCATGGGC
TCAGATTTCCTGAAGGCTGTGGAGACCTTGGTTCACAGCTTAAGACCACAACAGCATCCT
TTTTCCTTCAGCTCAGCTAATGTGCTACTGCAGAGCCAGCTCCTCAGACACTCCTCTCCT
CCTGGCTACCAAATGTCCTTCTCCACCTGGCCCCTGCTGCAGGCACGGATTCCATGGCAT
TCACTGGCCCCACTGGTCCATAGTGGAACTAACGTCAGTATTACTAGCCTAGTACTGCAA
AAGCTGGACTACCGGCTGCCCTCAAACTACACACAAGGGCTGTGGAACACCCCATACACC
ACCCCTGGGCTGATCCTTGTGGTCTCCATAACAGCAGACGGCCAGGCCTTCACGCAGGCA
GAAGTTATCATGGACTATGAAGACATGAATGGAACCCTTCACTGTGTGTTCTGGGACCAC
AGGGTCTTCCAGGGTCAGGGAGGTTGGTCAGATGAAGGGTGTGAGGTACATGCAGCCAAT
GCCAGCATCACTCAGTGCATTTGCCAGCATCTCACAGCCTTTTCCATCCTCATGTCCCAA
CACACTGTCCCAGAAAATCCCACCCTGGACCTGCTGAGCCAGGTGGGTACGGGGGCTTCC
GTCCTGGCTTTGCTCGTCTGCCTGGCTATTTACGGGCTGGTGTGGAGAGTGGTGGTCCGG
AACAAAGTCGCCTTCTTCCGCCATACCACCCTGTTTAACATGGTGATCTGCTTGCTGGTC
GCAGACACATGCTTCTTGGGAAGCCCCTTCCTTCCTTCAGGCTACCACAGCCTGATCTGC
CTAGTCACTGCTTTCCTGTGTCACTTTTTCTACCTGGCCACCTTTTTCTGGATGCTGGCA
CAGGCCCTGGTGCTGGCCCACCAGCTGCTTTTTGTCTTCCACCAGCTGTCCAAGCACGTA
GTCCTCTCACTGATGGTGATGCTGGGCTACCTGTGCCCACTGGGGTTTGCGGGTGTCACC
CTGGGCCTCTACTTGCCTCAAAGGAAATACCTGTGGGAGGGGAAATGCTTTCTGAACGGA
GGTGGCGTCATGCTGTACAGCTTCAGTGAGCCAGTGCTGGCCATCGTCGGTGTCAACGGG
CTGGTCCTTGTCATCGCTGTGCTGAAGCTGCTGCGACCTTCACTGTCAGAGGGGCCCACA
GTGGAGAAGCGCCAAGCTCTTGTGGGGGTGCTTAAAGCCCTGCTTATTCTCACACCCATC
TTTGGCCTCACTTGGGGACTGGGCGTGGCCACTCTGTTTGATGGCTCCATAGTCTCTCAC
TACGCCTTTTCCATTCTCAACAGCCTCCAGGGTGTCTTCATATTAGTGTTTGGCTGCCTC
ACAGACAAGAAGGTACTAGAGGCCTTGCGCAAACGATTGCGAGGCAGCCGGTCCTCTAAC
TCTGCCATCTCCATGGTCACAAATGAAACCTATACCTCAGAACACAGCAAAGAAAGAAGT
GAGCCTGCCAGTTATGAAGAAAGGATGACTGATTAA
Protein sequence predicted based on nucleotide sequence
MSSLALSQLLLAVTLPLLELEPTFVPTAQSELSPYGGKSGQQLNQYSGEGESVLVSSYVH LEFSSTAWPQELSKNFTLPTALAVLPPKTLTGLGLTTECRANHSGTSLCTCHPGYQWNAT LCSLYPHCWGRRSERDSCMCRSFHGPVTGYCQLLPPVPANLILDSQLQMHGNTLNLILLK KEEATNLRWFLRHSKNHTPISLWPGTNVLQTSSEGQSGLRVARMSRHWAGEYESIFEAQG FRWRLRQLVKVPLQEEEVVRLPDALSISCTASTGFQLSCCIPLTTDYTATWSPGEDSQAS LQKMSDSQCFVLAVPHCPATNTTYTCTLQSQNLPPLVAPVSITIIQDGDTTCPEDFSVVS WNVTKAGFVAQAPCPVNKKGVVKRLCGTDGIWRPVQNTCTEAKILTLCLKAKLLEGQGKP YEEVPWILSELQEQVGVASTPSDLWEMLHTVTLLAKVVAETSTELTGSALKDLLTTTDKI LDANISALWTLAQAQEPSMGSDFLKAVETLVHSLRPQQHPFSFSSANVLLQSQLLRHSSP PGYQMSFSTWPLLQARIPWHSLAPLVHSGTNVSITSLVLQKLDYRLPSNYTQGLWNTPYT TPGLILVVSITADGQAFTQAEVIMDYEDMNGTLHCVFWDHRVFQGQGGWSDEGCEVHAAN ASITQCICQHLTAFSILMSQHTVPENPTLDLLSQVGTGASVLALLVCLAIYGLVWRVVVR NKVAFFRHTTLFNMVICLLVADTCFLGSPFLPSGYHSLICLVTAFLCHFFYLATFFWMLA QALVLAHQLLFVFHQLSKHVVLSLMVMLGYLCPLGFAGVTLGLYLPQRKYLWEGKCFLNG GGVMLYSFSEPVLAIVGVNGLVLVIAVLKLLRPSLSEGPTVEKRQALVGVLKALLILTPI FGLTWGLGVATLFDGSIVSHYAFSILNSLQGVFILVFGCLTDKKVLEALRKRLRGSRSSN SAISMVTNETYTSEHSKERSEPASYEERMTD*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
18
End on reference sequence
2993
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
