DNASU Plasmid | Mmp1b (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Mmp1b

Gene Name: matrix metallopeptidase 1b (interstitial collagenase)

Original Clone ID:

FLH265700.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00081076

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1392nts

Open reading frame: 1 to 1392

ATGTCTAGCCTTCCTTTGCTGTTGCTTCTCTGGGCTGCCAGCTCATACAGTTTCCCTGTG
ATTCAGGATGGACTCCAGAAAAATGTGAAGACAGTCTGGAAATACCTGGAAAACTACTAC
AACTTGGGCAAAAACATGCAAGCTAAAAACGTGAATGGCAAGGAGGTGATGGCTGAAAAG
CTGAGGCAAATGCAGCAGTTATTTGGGCTGAAAGTAACTGGAAATTCAGATCCTGAAACC
CTGAGAGCTATGAAGAAGCCCAGGTGTGGGGTGCCTGATGTGGCCCCATATGCCATTACT
CACAACAATCCTCGTTGGACCAAAACACATCTGACTTACAGCATTTTAAACTACACACCA
TATTTGTCAAAAGCAGTTGTGGAAGATGCCATCGCGAGAGCCTTTAGAGTCTGGAGTGAT
GTGACACCACTTACGTTCCAAAGAGTCTTTGAGGAGGAAGGCGATATTGTGCTCTCCTTC
CACAGAGGAGACCATGGTGACCTCTACACATTTGATGGATCTAAATATCACTTTGCTCAT
GCTTTTCTGCCAGGCCTAGGTTTGGGGGGCAATGTTCATTATGATCTTGATCAGAAGTGG
ACCGACAACAATGAGGATTTCAACTTGTTCTATGTTACGGCTCATGAACTGGGTCACTCC
CTTGGACTCTCCCATTCTAATGATGAAGAGGCACTAATGTTCCCCAGCTACACATGGAGC
AATAAAGACTTTGTGCTAAACCAGGATGATATTAATCGCATCCAGGCCTTATATGGACCT
TCCCCAAATCCCATCCAGTTAACAGATGCAACACTGGATCCATGTAATAGTGGTCTAACC
TTTGATGCTATAATTACATATCGGGGGGAGGTGATATTCTTTAAAGACAGGTTCTACATT
CGGGTAATTAGCTTCCTGCCAGAACCTTTGATCGATGTGATAGACCTTATCTGGCCAAAT
CTGCCAGGTAAATTTGATGCAGCTTATGAAGTTAGTGGGGTAGATGAACTCCGCTTTTTC
AAAGGCAGCAAAGTATGGGCTGTTCAGGAACAGAATGTACTGGAAGGATTTCCCATGGAC
ATCCAGAGTTTCTTTGGCTTCCCTAGCAATGTGACAAACATTGATGCTGCTGTTTGTGAA
GAAGAGACTGGAAAAACATACTTCTTTGTTGACCACATGTACTGGAGATATGATGAAAAT
ACACGGTCCATGGATCCAGGTTACCCCAGATTAATAGCAGAAGACTTTCCTGGAATCGAC
TATAAAGTTGATGATGTTATCCAGAAAGAGGATAATTTCTATTTCTTTCACCAATCAATT
CAATACAGATTTAACCTCAAAACAAGAAGAATTGATGATTCCAGTGATATTAATACTTGG
TTCAACTGCTAA

Protein sequence predicted based on nucleotide sequence

MSSLPLLLLLWAASSYSFPVIQDGLQKNVKTVWKYLENYYNLGKNMQAKNVNGKEVMAEK
LRQMQQLFGLKVTGNSDPETLRAMKKPRCGVPDVAPYAITHNNPRWTKTHLTYSILNYTP
YLSKAVVEDAIARAFRVWSDVTPLTFQRVFEEEGDIVLSFHRGDHGDLYTFDGSKYHFAH
AFLPGLGLGGNVHYDLDQKWTDNNEDFNLFYVTAHELGHSLGLSHSNDEEALMFPSYTWS
NKDFVLNQDDINRIQALYGPSPNPIQLTDATLDPCNSGLTFDAIITYRGEVIFFKDRFYI
RVISFLPEPLIDVIDLIWPNLPGKFDAAYEVSGVDELRFFKGSKVWAVQEQNVLEGFPMD
IQSFFGFPSNVTNIDAAVCEEETGKTYFFVDHMYWRYDENTRSMDPGYPRLIAEDFPGID
YKVDDVIQKEDNFYFFHQSIQYRFNLKTRRIDDSSDINTWFNC*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1426

Start on reference sequence 35

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Mmp1b

Symbol Nomenclature :

Mmp1b

SYNONYM :

Mcol-B; Mcolb

Designation :

MMP-1b|collagenase-like B|interstitial collagenase B|matrix metalloproteinase 1b (interstitial collagenase)|matrix metalloproteinase-1b

Full Nomenclature :

matrix metallopeptidase 1b (interstitial collagenase)

GENEID :

83996

GI :

151555538

GenBank Accession :

BC148744

Mouse Genome Informatics :

1933847

Target GenBank:

NM_032007

Protein

SMART domain :

HX : Hemopexin-like repeats.

SMART domain :

ZnMc : Zinc-dependent metalloprotease

Clone

Cloning Information :

265700

HIP Master Clone ID :

197798

IMAGE ID :

100015760

Original Clone ID :

FLH265700.01X

Organism

Species Specific ID:

83996

Genome

Chromosome :

Map Location :

9 A1|9

Reagents

Labome :

Mmp1b-antibody