Gene:
Gene Symbol:
Ipo7
Gene Name:
importin 7
Original Clone ID:
FLH265748.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00081124
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3114nts
Open reading frame: 1 to 3114
ATGGATCCCAACACCATCATCGAGGCCCTCCGGGGCACCATGGACCCAGCCCTGCGCGAG
GCCGCTGAGCGCCAGCTCAATGAAGCACACAAGTCTCTGAATTTCGTTTCAACACTGCTC
CAGATTACTATGTCAGAACAACTGGATTTACCTGTGAGACAGGCAGGTGTTATTTATTTA
AAAAATATGATAACACAATATTGGCCTGATCGGGAAGCCACACCAGGGGACATCGCTCCA
TATACTATTCCAGAAGAAGACCGGCATTGTATTCGAGAAAATATTGTAGAAGCCATTATC
CACTCACCTGAGCTTATCAGGGTACAGCTTACTACGTGCATCCATCATATCATCAAACAT
GACTATCCAAGCCGCTGGACTGCCATTGTGGACAAGATTGGCTTTTATCTTCAGTCTGAT
AACAGTGCTTGTTGGCTGGGAATCCTTCTTTGCCTTTATCAGCTTGTGAAAAATTATGAG
TACAAGAAACCAGAAGAGCGAAGTCCGTTAGTGGCAGCCATGCAGCACTTTCTGCCAGTT
CTGAAGGATCGCTTTATCCAGCTGCTTTCTGACCAATCCGACCAGTCTGTCCTCATTCAA
AAGCAGATATTCAAGATCTTCTATGCTCTTGTTCAGTATACACTACCCCTGGAGCTGATA
AACCAACAGAATCTAACTGAATGGGTAGAAATTTTGAAGACAGTTGTGAATAGGGATGTA
CCTAATGAAACACTTCAAGTTGAAGAAGATGATAGACCTGAGTTACCCTGGTGGAAATGT
AAGAAGTGGGCCTTACATATTTTAGCAAGACTTTTTGAAAGATATGGAAGCCCTGGGAAT
GTTTCTAAGGAGTATAATGAGTTTGCTGAAGTCTTTCTGAAGGCCTTTGCTGTTGGTGTC
CAGCAAGTTTTATTGAAAGTGTTATATCAGTACAAGGAAAAACAATATATGGCGCCTCGA
GTTTTACAGCAGACATTAAATTATATTAATCAAGGAGTTTCCCATGCACTTACGTGGAAG
AACCTGAAGCCCCATATACAAGGCATTATCCAAGATGTTATTTTTCCATTGATGTGCTAT
ACAGATGCTGATGAGGAGCTCTGGCAAGAAGATCCTTATGAATATATACGCATGAAGTTC
GATGTGTTTGAAGATTTCATTTCTCCTACTACTGCTGCTCAAACACTTTTGTTTACAGCC
TGTAGCAAAAGGAAAGAGGTACTGCAAAAGACTATGGGATTTTGTTACCAAATTCTTACT
GAACCCAATGCTGATCCTCGGAAAAAAGATGGAGCCTTGCATATGATTGGCTCTTTAGCT
GAAATACTTCTAAAGAAAAAGATCTACAAAGATCAAATGGAATACATGTTGCAAAATCAT
GTATTTCCTCTCTTCAGCAGTGAGCTAGGTTACATGAGAGCAAGGGCTTGCTGGGTTCTT
CACTATTTTTGTGAAGTGAAGTTCAAAAGTGATCAAAATCTCCAAACAGCTTTAGAGCTG
ACAAGAAGATGTCTGATTGATGATCGAGAAATGCCTGTGAAAGTGGAAGCTGCCATTGCA
CTACAGGTGTTGATCAGCAATCAAGAAAAAGCTAAAGAGTACATCACTCCATTTATCAGA
CCTGTCATGCAGGCTCTTCTTCATATTATAAGAGAAACAGAAAATGATGATCTTACAAAT
GTAATTCAGAAAATGATCTGTGAATATAGTGAAGAAGTTACTCCTATTGCTGTAGAAATG
ACACAACATCTGGCAATGACATTTAACCAAGTAATCCAGACTGGGCCAGATGAGGAAGGA
AGTGATGACAAAGCAGTTACTGCTATGGGAATTCTGAACACAATTGATACACTTCTGAGT
GTGGTTGAAGATCACAAAGAGATAACCCAGCAGCTTGAGGGAATCTGCTTACAAGTCATT
GGGACCGTTCTACAACAGCATGTCTTAGAATTCTATGAGGAGATCTTTTCTTTGGCACAT
AGTCTGACATGTCAACAAGTGTCTCCTCAGATGTGGCAGTTACTTCCCCTTGTGTTTGAG
GTATTTCAGCAAGATGGCTTTGATTACTTTACAGACATGATGCCTCTTCTACATAACTAT
GTAACAGTTGATACAGACACTCTTCTCTCTGATACCAAGTATCTGGAGATGATATACAGT
ATGTGTAAAAAGGTTCTTACTGGAGTCGCAGGAGAAGATGCGGAATGCCATGCGGCAAAA
TTGTTAGAGGTCATTATTCTGCAGTGCAAAGGCCGGGGCATTGATCAGTGTATACCATTA
TTCGTGGAAGCAGCTTTAGAAAGACTGACCAGAGAGGTTAAAACAAGTGAACTCAGAACA
ATGTGCCTGCAGGTTGCAATTGCAGCATTGTATTATAATCCACACCTACTGCTTAATACT
TTAGAAAATCTTCGCTTCCCAAATAATGTTGAACCTGTTACAAATCATTTTATCACACAA
TGGCTTAATGACGTTGACTGTTTTCTGGGGCTTCATGACAGGAAGATGTGTGTTCTTGGC
CTATGCGCCCTTATTGATATGGAACAAATACCACAGGTTTTAAATCAGGTTTCAGGACAG
ATTTTGCCAGCTTTTATCCTTTTATTTAATGGATTGAAAAGAGCATATGCCTGCCATGCA
GAACACGAGAATGACAGTGACGATGATGAGGATGCGGAAGATGATGATGAAACTGAAGAA
CTGGGGAGTGATGAAGATGATATTGATGAAGATGGACAAGAATATTTGGAGATCCTGGCT
AAACAAGCTGGTGAGGATGGAGATGATGAAGATTGGGAAGAAGATGATGCTGAGGAGACT
GCTCTGGAAGGCTATTCCACAATCATCGATGATGAAGACAACCCTGTTGATGAGTATCAA
ATCTTTAAAGCTATATTTCAAACTATTCAAAATCGCAATCCTGTATGGTATCAGGCTTTG
ACTCACGGTCTTAATGAAGAGCAAAGGAAACAGTTACAGGACATAGCAACTCTAGCTGAT
CAAAGAAGAGCAGCCCATGAGTCCAAAATGATTGAAAAGCATGGAGGATACAAATTCAGT
GCACCAGTTGTGCCAAGTTCTTTCAATTTTGGAGGCCCAGCACCGGGGATGAAT
Protein sequence predicted based on nucleotide sequence
MDPNTIIEALRGTMDPALREAAERQLNEAHKSLNFVSTLLQITMSEQLDLPVRQAGVIYL KNMITQYWPDREATPGDIAPYTIPEEDRHCIRENIVEAIIHSPELIRVQLTTCIHHIIKH DYPSRWTAIVDKIGFYLQSDNSACWLGILLCLYQLVKNYEYKKPEERSPLVAAMQHFLPV LKDRFIQLLSDQSDQSVLIQKQIFKIFYALVQYTLPLELINQQNLTEWVEILKTVVNRDV PNETLQVEEDDRPELPWWKCKKWALHILARLFERYGSPGNVSKEYNEFAEVFLKAFAVGV QQVLLKVLYQYKEKQYMAPRVLQQTLNYINQGVSHALTWKNLKPHIQGIIQDVIFPLMCY TDADEELWQEDPYEYIRMKFDVFEDFISPTTAAQTLLFTACSKRKEVLQKTMGFCYQILT EPNADPRKKDGALHMIGSLAEILLKKKIYKDQMEYMLQNHVFPLFSSELGYMRARACWVL HYFCEVKFKSDQNLQTALELTRRCLIDDREMPVKVEAAIALQVLISNQEKAKEYITPFIR PVMQALLHIIRETENDDLTNVIQKMICEYSEEVTPIAVEMTQHLAMTFNQVIQTGPDEEG SDDKAVTAMGILNTIDTLLSVVEDHKEITQQLEGICLQVIGTVLQQHVLEFYEEIFSLAH SLTCQQVSPQMWQLLPLVFEVFQQDGFDYFTDMMPLLHNYVTVDTDTLLSDTKYLEMIYS MCKKVLTGVAGEDAECHAAKLLEVIILQCKGRGIDQCIPLFVEAALERLTREVKTSELRT MCLQVAIAALYYNPHLLLNTLENLRFPNNVEPVTNHFITQWLNDVDCFLGLHDRKMCVLG LCALIDMEQIPQVLNQVSGQILPAFILLFNGLKRAYACHAEHENDSDDDEDAEDDDETEE LGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQ IFKAIFQTIQNRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAHESKMIEKHGGYKFS APVVPSSFNFGGPAPGMN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3117
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
