DNASU Plasmid | Tmtc3 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Tmtc3

Gene Name: transmembrane and tetratricopeptide repeat containing 3

Original Clone ID:

FLH265761.01F

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00081137

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1974nts

Open reading frame: 1 to 1974

ATGCTTGAAGGGAAGATGGCTGATATTAACTTCAAAGAAGTGACTTTAATAGTCAGCGTG
GTTGCCGCCTGCTACTGGAACAGCCTGTTCTGTGGGTTTGTTTTTGATGATGTTTCAGCA
ATATTGGATAATAAAGACCTGCATCCATCTACACCTTTAAAAACTTTATTTCAGAATGAC
TTCTGGGGAACTCCTATGTCTGAGGAGAGAAGCCACAAGTCATACAGGCCCTTAACGGTG
TTGACATTTCGCTTAAATTATCTCCTCAGTGAGCTGAAACCCATGTCTTACCACCTCCTG
AACACAGTGTTCCACGCTGTCGTCAGCGTGATCTTCCTTAAGGTCTGCAGACTCTTTCTG
GATAAGAGGAGCAGCATGATTGCGGCTCTGCTCTTCGCAGTGCACCCAATCCATACCGAG
GCAGTCACAGGTGTTGTAGGAAGAGCGGAACTCTTATCATCTGTCTTTTTCCTAGCTGCC
TTTCTCTCGTATACTAAGTCAAAAGGACCAGATAATTCCATAGTGTGGACCCCGATTGTA
TTAACCGTGTTTTTAGTGGCTGTTGCAACATTGTGTAAGGAACAAGGAATAACGGTTGTC
GGAATTTGTTGTGTATATGAAGTATTTGTGGCCCAAGGGTACACCTTGCCGATGTTATGC
ACCGTTGCCGGGCAGTTTCTGCGCGGGAAGGGCAGCATTCCGTTATCTATGCTGCAGACA
CTGGTGAAGCTCATTGTCCTGATGCTCAGTACCTTGCTACTCGTTGTGGTCAGAGTCCAA
GTTATTCAGTCACAACTTCCAGTGTTTACCAGGTTTGATAATCCAGCTGCTGTAAGCCCA
ACCCCGACACGACAGCTGACTTTTAACTATCTCCTTCCTGTGAATGCCTGGCTTCTGCTG
AATCCTTCGGAGCTCTGCTGTGATTGGACCATGGGGACAATACCACTGATAGAATCATTT
CTAGATGTTCGAAATCTCGCCACTTTTGCTTTCTTTTGCTTTCTGGGGGCTTTGGGAATA
TTCAGTCTCAGATACCCTGGTGACTCCTCAAAGACTGTCCTAATGGCGCTTTGTTTAATG
GCGTTACCATTTATTCCCGCATCAAACCTGTTCTTTCCTGTTGGATTTGTGGTTGCTGAG
CGAGTACTATATGTTCCTAGCATGGGGTTCTGTATTTTAGTAGCCCATGGATGGCAAAAA
ATTTCAAACAAAAGTGTGCTGAAAAAGCTCTCGTGGGTTTGTCTGTCCATGGTGATACTA
ACCCATGCCTTGAAAACACTTCATAGAAATTGGGACTGGGAGTCAGAATATACATTGTTT
ATGTCAGCCCTAAAGGTGAATAAAAACAATGCCAAATTATGGAATAATGTGGGTCATGCT
CTGGAGAATGAGAAGAACTTTGAGAAAGCTTTGAAATACTTCTTGCAGGCTACCCATGTT
CAGCCAGATGACATCGGTGCCCACATGAATGTAGGAAGAACTTATAAAAACTTAAACAGA
ACTAGAGAAGCTGAAGCGTCTTACATGCTGGCTAAATCACTGATGCCTCAGATTATCCCT
GGTAAAAAATATGCAGCCAGAATTGCCCCTAATCACCTAAACGTTTATATCAATCTGGCC
AACCTTATTCGAGCAAATGAGTCCCGCCTGGAGGAAGCGGACCAGCTGTACCGACAGGCC
ATCAGCATGAGGCCAGACTTCAAACAGGCTTACATTAGCAGGGGAGAGTTGCTTTTAAAA
ATGAATAAGCCTCTCAAAGCAAAGGAAGCATATCTTAAAGCACTAGAGCTGGACAGAAAT
AATGCAGATCTCTGGTACAACTTGGCAATTGTTTATATTGAACTTAAAGAACCAAATGAA
GCTCTGAAGAACTTTAATCGAGCTTTGGAACTGAACCCTAAACATAAGCTAGCACTGTTC
AATTCTGCTATTTTAATGCAGGAATCAGGTAAGTTTCCTGAAAATGTCTCTATT

Protein sequence predicted based on nucleotide sequence

MLEGKMADINFKEVTLIVSVVAACYWNSLFCGFVFDDVSAILDNKDLHPSTPLKTLFQND
FWGTPMSEERSHKSYRPLTVLTFRLNYLLSELKPMSYHLLNTVFHAVVSVIFLKVCRLFL
DKRSSMIAALLFAVHPIHTEAVTGVVGRAELLSSVFFLAAFLSYTKSKGPDNSIVWTPIV
LTVFLVAVATLCKEQGITVVGICCVYEVFVAQGYTLPMLCTVAGQFLRGKGSIPLSMLQT
LVKLIVLMLSTLLLVVVRVQVIQSQLPVFTRFDNPAAVSPTPTRQLTFNYLLPVNAWLLL
NPSELCCDWTMGTIPLIESFLDVRNLATFAFFCFLGALGIFSLRYPGDSSKTVLMALCLM
ALPFIPASNLFFPVGFVVAERVLYVPSMGFCILVAHGWQKISNKSVLKKLSWVCLSMVIL
THALKTLHRNWDWESEYTLFMSALKVNKNNAKLWNNVGHALENEKNFEKALKYFLQATHV
QPDDIGAHMNVGRTYKNLNRTREAEASYMLAKSLMPQIIPGKKYAARIAPNHLNVYINLA
NLIRANESRLEEADQLYRQAISMRPDFKQAYISRGELLLKMNKPLKAKEAYLKALELDRN
NADLWYNLAIVYIELKEPNEALKNFNRALELNPKHKLALFNSAILMQESGKFPENVSI

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 202

End on reference sequence 2178

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Tmtc3

Symbol Nomenclature :

Tmtc3

SYNONYM :

9130014E20Rik; B130008E12Rik; mSmile

Designation :

transmembrane and TPR repeat-containing protein 3

Full Nomenclature :

transmembrane and tetratricopeptide repeat containing 3

GENEID :

237500

GI :

146327259

GenBank Accession :

BC141573

Mouse Genome Informatics :

3036255

Vega :

OTTMUSG00000034150

Target GenBank:

NM_001033332

Protein

SMART domain :

TPR : Tetratricopeptide repeats

UniProt :

Q8BRH0

Clone

Cloning Information :

265761

HIP Master Clone ID :

197859

IMAGE ID :

100014528

Original Clone ID :

FLH265761.01F

Organism

Species Specific ID:

237500

Genome

Chromosome :

Map Location :

10 D1|10

Ensembl :

ENSMUSG00000036676

Reagents

Labome :

Tmtc3-antibody