Gene:
Gene Symbol:
Lig4
Gene Name:
ligase IV, DNA, ATP-dependent
Original Clone ID:
FLH265808.01F
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00081184
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2733nts
Open reading frame: 1 to 2733
ATGGCTTCCTCACAAACTTCACAAACTGTTGCAGCTCATGTCCCCTTTGCAGACTTATGT
TCCACACTAGAACGGATACAGAAAGGTAAAGACCGTGCAGAAAAAATCAGGCACTTCAAG
GAGTTTTTGGATTCGTGGAGAAAATTTCATGATGCCCTTCATAAGAACAGAAAGGACGTT
ACAGACTCTTTTTACCCTGCAATGAGACTCATTCTCCCCCAGTTAGAAAGAGAGAGGATG
GCTTATGGAATCAAAGAAACCATGCTCGCTAAGCTCTACATCGAATTGCTGAACTTACCA
CGAGAAGGCAAGGATGCCCAGAAGCTCCTCAATTACCGAACCCCCAGTGGAGCTCGCACG
GATGCTGGGGACTTTGCCATGATTGCATACTTTGTTTTGAAGCCAAGGTGCTTACAGAAA
GGAAGCTTAACCATACAGCAGGTAAATGAACTCTTAGACTTAGTTGCCAGCAATAACTCT
GGCAAAAAAAAAGACCTAGTGAAAAAGAGCCTTCTTCAGTTAATAACCCAGAGTTCAGCA
CTGGAGCAAAAATGGCTGATTCGCATGATTATCAAAGACTTAAAGCTTGGCATCAGTCAG
CAAACAATATTTTCCATTTTCCACAATGATGCAGTTGAGTTGCACAACGTCACCACAGAT
CTGGAAAAGGTCTGCAGGCAGCTGCATGACCCCTCTGTAGGGCTTAGTGACATCTCTATC
ACTCTGTTTTCTGCCTTTAAGCCAATGCTAGCTGCTGTAGCAGACGTGGAGCGTGTGGAG
AAGGACATGAAGCAGCAGAGTTTCTACATCGAAACTAAGCTTGATGGTGAGCGCATGCAG
ATGCACAAAGATGGCGCGCTGTACCGGTACTTCTCCAGAAACGGTTACAACTATACCGAC
CAGTTTGGTGAATCTCCACAGGAAGGCTCTCTCACCCCATTTATTCACAATGCGTTCGGG
ACAGATGTGCAAGCGTGCATCCTTGACGGTGAGATGATGGCCTACAACCCAACAACACAG
ACTTTCATGCAGAAGGGGGTCAAGTTTGATATCAAAAGGATGGTGGAAGATTCTGGCCTA
CAGACTTGTTACTCTGTTTTTGACGTACTGATGGTTAATAAGAAGAAGCTAGGGCGCGAG
ACTCTTAGGAAGAGGTATGAGATCCTTAGTAGCACTTTCACACCCATACAAGGCCGCATA
GAAATAGTGCAGAAAACTCAAGCTCATACAAAGAAGGAAGTAGTGGATGCATTAAATGAT
GCCATAGACAAGAGAGAAGAGGGGATCATGGTTAAACACCCTCTGTCCATTTACAAGCCA
GACAAAAGAGGTGAAGGGTGGCTAAAGATTAAACCAGAGTACGTCAGTGGACTAATGGAT
GAATTAGACGTCCTAATTGTGGGGGGCTACTGGGGTAAAGGTTCACGAGGTGGCATGATG
TCTCACTTTTTGTGTGCAGTGGCAGAGACACCACCTCCTGGTGACAGGCCATCTGTATTC
CACACTCTGTGCCGTGTTGGGTCTGGTTACACCATGAAAGAACTCTATGACCTGGGCTTG
AAATTGGCAAAATACTGGAAGCCTTTTCATAAGAAATCTCCACCAAGTAGCATTCTGTGT
GGTACAGAGAAGCCGGAAGTGTACATAGAGCCTCAGAACTCTGTCATTGTTCAGATCAAG
GCAGCTGAGATCGTCCCCAGTGACATGTACAAGACTGGCTCCACCCTGCGCTTCCCACGC
ATCGAAAAGATCAGAGATGACAAAGAGTGGCACGAATGTATGACACTGGGTGACTTGGAG
CAGCTGAGGGGGAAAGCATCTGGGAAGCTTGCCACGAAACACCTTCATGTAGGTGATGAT
GATGAACCTAGAGAAAAAAGGCGGAAGCCCATCTCCAAGACGAAGAAAGCCATTAGAATC
ATTGAACACTTAAAAGCACCCAACCTTTCTAACGTAAACAAAGTTTCCAATGTATTTGAA
GATGTTGAGTTTTGTGTTATGAGTGGATTAGATGGTTATCCAAAGGCTGACCTAGAGAAC
AGAATTGCAGAATTCGGTGGTTATATAGTACAGAATCCAGGCCCGGATACATACTGTGTT
ATTGCAGGTTCTGAGAACGTTAGAGTGAAAAACATTATTTCTTCAGATAAAAATGATGTT
GTCAAGCCCGAGTGGCTTTTAGAGTGTTTTAAGACAAAAACATGCGTGCCGTGGCAACCT
CGCTTTATGATTCACATGTGCCCGTCGACAAAGCAGCATTTTGCCCGTGAGTATGACTGC
TATGGTGATAGCTATTTTGTTGACACAGATTTGGATCAATTGAAAGAAGTGTTTCTAGGA
ATTAAACCCAGTGAGCAGCAGACTCCTGAAGAAATGGCCCCTGTGATTGCTGACTTAGAA
TGTCGTTATTCCTGGGACCACTCTCCTCTCAGTATGTTTCGACATTACACCATTTATTTG
GACTTGTATGCTGTTATTAATGACTTGAGTTCCAGAATTGAAGCCACGAGATTAGGTATT
ACAGCCCTTGAGCTGCGGTTTCATGGAGCAAAGGTGGTTTCCTGCTTATCTGAAGGGGTA
TCTCATGTTATCATTGGGGAGGATCAGAGACGAGTTACTGACTTTAAAATATTCAGAAGA
ATGCTTAAGAAAAAGTTTAAAATCCTGCAAGAAAGTTGGGTGTCCGATTCAGTAGACAAG
GGCGAACTGCAGGAGGAAAACCAGTATTTGCTT
Protein sequence predicted based on nucleotide sequence
MASSQTSQTVAAHVPFADLCSTLERIQKGKDRAEKIRHFKEFLDSWRKFHDALHKNRKDV TDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPREGKDAQKLLNYRTPSGART DAGDFAMIAYFVLKPRCLQKGSLTIQQVNELLDLVASNNSGKKKDLVKKSLLQLITQSSA LEQKWLIRMIIKDLKLGISQQTIFSIFHNDAVELHNVTTDLEKVCRQLHDPSVGLSDISI TLFSAFKPMLAAVADVERVEKDMKQQSFYIETKLDGERMQMHKDGALYRYFSRNGYNYTD QFGESPQEGSLTPFIHNAFGTDVQACILDGEMMAYNPTTQTFMQKGVKFDIKRMVEDSGL QTCYSVFDVLMVNKKKLGRETLRKRYEILSSTFTPIQGRIEIVQKTQAHTKKEVVDALND AIDKREEGIMVKHPLSIYKPDKRGEGWLKIKPEYVSGLMDELDVLIVGGYWGKGSRGGMM SHFLCAVAETPPPGDRPSVFHTLCRVGSGYTMKELYDLGLKLAKYWKPFHKKSPPSSILC GTEKPEVYIEPQNSVIVQIKAAEIVPSDMYKTGSTLRFPRIEKIRDDKEWHECMTLGDLE QLRGKASGKLATKHLHVGDDDEPREKRRKPISKTKKAIRIIEHLKAPNLSNVNKVSNVFE DVEFCVMSGLDGYPKADLENRIAEFGGYIVQNPGPDTYCVIAGSENVRVKNIISSDKNDV VKPEWLLECFKTKTCVPWQPRFMIHMCPSTKQHFAREYDCYGDSYFVDTDLDQLKEVFLG IKPSEQQTPEEMAPVIADLECRYSWDHSPLSMFRHYTIYLDLYAVINDLSSRIEATRLGI TALELRFHGAKVVSCLSEGVSHVIIGEDQRRVTDFKIFRRMLKKKFKILQESWVSDSVDK GELQEENQYLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3058
Start on reference sequence
323
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
