Gene:
Gene Symbol:
KCNH4
Gene Name:
potassium voltage-gated channel, subfamily H (eag-related), member 4
Original Clone ID:
FLH265835.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081211
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCGGTCATGAAGGGGTTGCTGGCCCCGCAAAACACCTTCCTGGACACCATCGCCACC
CGTTTTGACGGAACGCACAGCAACTTCCTGCTGGCCAACGCACAGGGCACACGGGGCTTT
CCCATCGTCTACTGCTCCGACGGCTTCTGCGAGCTCACAGGCTACGGTCGCACCGAGGTC
ATGCAGAAGACCTGCAGCTGCCGTTTCCTCTACGGCCCAGAGACCAGTGAGCCAGCCCTG
CAGCGTCTGCACAAAGCCCTGGAGGGCCACCAGGAGCACCGGGCTGAAATCTGCTTCTAC
CGCAAGGATGGCTCAGCCTTTTGGTGCCTCCTGGACATGATGCCCATCAAGAATGAGATG
GGGGAGGTCGTGCTGTTCCTCTTTTCCTTCAAGGATATCACTCAGAGTGGAAGCCCAGGA
CTTGGCCCCCAAGGAGGCCGCGGGGACAGTAATCACGAAAACTCCCTTGGTAGAAGGGGA
GCCACCTGGAAATTTCGGTCTGCCAGAAGACGGAGCCGTACTGTCCTACACCGACTGACC
GGCCACTTTGGCCGCCGGGGCCAGGGAGGCATGAAGGCCAATAATAACGTGTTTGAGCCA
AAGCCATCAGTGCCCGAGTACAAGGTGGCCTCCGTGGGGGGGTCTCGCTGCCTCCTCCTC
CACTACAGCGTCTCCAAGGCCATCTGGGACGGCCTTATCCTCCTTGCCACCTTCTACGTT
GCGGTCACCGTCCCCTACAATGTCTGTTTCTCGGGTGACGATGACACCCCCATCACTTCG
CGACACACCCTTGTCAGCGACATCGCCGTGGAAATGCTCTTCATCCTAGATATCATCCTG
AACTTCCGCACCACCTATGTGTCCCAGTCCGGCCAGGTAATCTCTGCTCCTCGTTCCATT
GGCCTCCACTACCTGGCCACCTGGTTCTTCATCGACCTTATTGCTGCTCTGCCCTTTGAC
CTGCTTTACATCTTCAACATCACCGTGACCTCGCTGGTGCACCTACTGAAGACAGTGCGG
CTGTTGCGGCTGCTGCGGCTGCTGCAGAAGCTGGAGCGGTACTCTCAGTGCAGTGCTGTG
GTGCTCACGCTGCTCATGTCGGTCTTTGCGCTCCTTGCCCACTGGATGGCCTGCATCTGG
TATGTCATCGGGCGCCGGGAGATGGAGGCCAATGACCCGCTGCTCTGGGACATTGGCTGG
TTGCATGAGTTGGGCAAGCGTCTGGAGGTGCCCTATGTCAATGGCTCGGTGGGCGGCCCA
TCACGGCGCAGCGCCTACATCGCGGCACTGTACTTCACTCTAAGCAGCCTCACCAGTGTG
GGCTTTGGCAACGTGTGTGCCAACACCGACGCGGAGAAGATCTTCTCCATCTGCACGATG
CTCATAGGCGCCCTGATGCACGCTGTGGTGTTCGGGAACGTGACAGCCATCATCCAGCGC
ATGTACTCGCGCCGCTCGCTCTACCACAGCCGCATGAAGGACCTCAAGGACTTCATCCGT
GTGCACCGCCTGCCGCGGCCGCTCAAGCAGCGCATGCTCGAATACTTCCAGACCACGTGG
GCCGTCAACAGCGGCATCGACGCCAACGAGTTACTGCGTGACTTCCCAGACGAGCTGAGA
GCTGACATTGCTATGCACCTGAATCGGGAGATCCTGCAGCTGCCGTTGTTCGGGGCAGCG
AGCAGGGGCTGCCTGCGGGCCCTATCGCTGCACATCAAGACCTCGTTCTGCGCTCCGGGC
GAGTACCTGTTGCGCCGTGGGGATGCCCTGCAGGCACATTACTATGTCTGCTCCGGCTCG
CTTGAGGTGCTCCGAGACAACATGGTGCTGGCCATCCTGGGGAAGGGGGACCTGATTGGA
GCAGATATCCCTGAGCCGGGGCAGGAGCCTGGGTTGGGAGCAGACCCAAACTTCGTGCTA
AAGACCAGTGCTGATGTGAAAGCTCTGACCTACTGTGGCCTGCAGCAGCTGAGCAGCCGA
GGGCTGGCTGAGGTCCTGAGGCTCTATCCTGAGTATGGGGCTGCCTTCCGGGCTGGCCTG
CCCCGGGACCTCACCTTCAACCTGCGCCAGGGCTCTGACACCAGTGGCCTCAGCCGCTTT
TCCCGATCCCCTCGCCTCTCCCAGCCCCGCTCAGAAAGCCTCGGCTCCTCCTCAGACAAG
ACGCTGCCATCCATCACAGAGGCCGAGAGTGGCGCGGAGCCTGGGGGTGGTCCCAGGCCC
CGACGGCCCCTCCTGCTGCCCAACCTCAGCCCAGCACGGCCTCGGGGCTCCCTGGTCAGC
CTTTTGGGCGAGGAGCTGCCCCCATTCTCAGCCCTTGTCTCCTCTCCTTCCTTATCCCCA
TCCCTGTCCCCTGCCCTGGCTGGCCAGGGCCACAGTGCCTCCCCTCACGGCCCCCCCAGG
TGCTCTGCTGCCTGGAAGCCCCCTCAGCTTCTCATTCCCCCACTGGGAACCTTTGGACCT
CCGGACCTCAGTCCCCGGATAGTGGATGGCATTGAGGACTCTGGCAGCACAGCTGAGGCC
CCTTCATTCCGATTCAGCAGGAGGCCTGAACTGCCAAGGCCCCGCTCCCAGGCGCCCCCT
ACAGGGACCAGGCCCAGCCCAGAATTGGCCAGTGAGGCTGAGGAGGTGAAGGAAAAGGTT
TGCCGGCTGAACCAGGAGATCTCTCGTCTCAATCAGGAGGTGTCTCAGCTTAGCCGGGAG
CTGCGGCACATCATGGGCCTGCTGCAGGCCAGGCTGGGTCCCCCAGGCCACCCAGCAGGC
TCCGCTTGGACCCCAGACCCTCCTTGTCCACAGCTGAGGCCACCATGCCTCTCTCCTTGT
GCGTCCAGACCACCACCCAGCCTCCAGGATACTACGCTTGCTGAAGTTCACTGCCCAGCC
AGTGTGGGGACCATGGAGACAGGGACTGCGCTCCTGGACTTGAGACCTTCCATATTGCCC
CCCTACCCCTCAGAGCCTGACCCTCTGGGACCCTCTCCAGTGCCAGAGGCCTCACCCCCA
ACCCCAAGCCTCTTGAGGCACAGTTTCCAGTCCAGGTCAGACACGTTCCAC
Protein sequence predicted based on nucleotide sequence
MPVMKGLLAPQNTFLDTIATRFDGTHSNFLLANAQGTRGFPIVYCSDGFCELTGYGRTEV MQKTCSCRFLYGPETSEPALQRLHKALEGHQEHRAEICFYRKDGSAFWCLLDMMPIKNEM GEVVLFLFSFKDITQSGSPGLGPQGGRGDSNHENSLGRRGATWKFRSARRRSRTVLHRLT GHFGRRGQGGMKANNNVFEPKPSVPEYKVASVGGSRCLLLHYSVSKAIWDGLILLATFYV AVTVPYNVCFSGDDDTPITSRHTLVSDIAVEMLFILDIILNFRTTYVSQSGQVISAPRSI GLHYLATWFFIDLIAALPFDLLYIFNITVTSLVHLLKTVRLLRLLRLLQKLERYSQCSAV VLTLLMSVFALLAHWMACIWYVIGRREMEANDPLLWDIGWLHELGKRLEVPYVNGSVGGP SRRSAYIAALYFTLSSLTSVGFGNVCANTDAEKIFSICTMLIGALMHAVVFGNVTAIIQR MYSRRSLYHSRMKDLKDFIRVHRLPRPLKQRMLEYFQTTWAVNSGIDANELLRDFPDELR ADIAMHLNREILQLPLFGAASRGCLRALSLHIKTSFCAPGEYLLRRGDALQAHYYVCSGS LEVLRDNMVLAILGKGDLIGADIPEPGQEPGLGADPNFVLKTSADVKALTYCGLQQLSSR GLAEVLRLYPEYGAAFRAGLPRDLTFNLRQGSDTSGLSRFSRSPRLSQPRSESLGSSSDK TLPSITEAESGAEPGGGPRPRRPLLLPNLSPARPRGSLVSLLGEELPPFSALVSSPSLSP SLSPALAGQGHSASPHGPPRCSAAWKPPQLLIPPLGTFGPPDLSPRIVDGIEDSGSTAEA PSFRFSRRPELPRPRSQAPPTGTRPSPELASEAEEVKEKVCRLNQEISRLNQEVSQLSRE LRHIMGLLQARLGPPGHPAGSAWTPDPPCPQLRPPCLSPCASRPPPSLQDTTLAEVHCPA SVGTMETGTALLDLRPSILPPYPSEPDPLGPSPVPEASPPTPSLLRHSFQSRSDTFH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
334
End on reference sequence
3387
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
