Gene:
Gene Symbol:
HELQ
Gene Name:
helicase, POLQ-like
Original Clone ID:
FLH265898.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081274
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGATGAATGTGGTTCCCGCATCCGCCGGCGGGTGTCTCTCCCCAAAAGGAACCGTCCA
AGCTTGGGGTGTATTTTTGGCGCTCCCACCGCGGCCGAGCTCGAGCCCGGAGATGAGGGG
AAAGAGGAGGAGGAAATGGTGGCTGAGAACAGGAGGCGGAAAACCGCGGGCGTACTGCCG
GTTGAGGTACAGCCCCTTCTACTCTCAGATTCCCCGGAATGTCTCGTCCTTGGAGGTGGT
GATACAAACCCGGACCTCCTACGTCACATGCCCACTGACAGAGGGGTGGGAGACCAGCCT
AATGACAGTGAAGTGGACATGTTTGGTGACTATGATAGCTTTACTGAAAACTCCTTTATA
GCTCAAGTTGACGACCTGGAACAAAAATATATGCAACTCCCTGAACATAAGAAACATGCT
ACAGACTTTGCCACTGAAAATCTTTGCTCGGAAAGTATCAAAAACAAACTCAGCATTACT
ACCATAGGCAACCTTACTGAATTACAAACTGATAAGCACACAGAGAACCAGAGTGGATAT
GAAGGTGTCACTATTGAACCTGGAGCTGATCTTTTGTATGATGTACCTTCCTCACAGGCT
ATATACTTTGAAAATTTGCAGAACTCTTCAAATGATTTGGGTGATCATTCTATGAAAGAA
AGGGATTGGAAGTCATCCTCTCACAACACTGTGAATGAGGAACTGCCCCATAATTGCATA
GAGCAACCCCAGCAAAATGATGAGTCCTCTTCCAAAGTCAGAACTAGTTCAGATATGAAC
AGGAGAAAAAGTATTAAAGATCATCTAAAAAATGCCATGACTGGAAATGCGAAGGCCCAG
ACACCAATATTTTCTAGAAGTAAACAGCTCAAAGACACTCTCCTATCTGAGGAAATTAAT
GTTGCTAAGAAAACAGTTGAGTCATCATCAAATGACCTTGGTCCTTTTTATTCATTACCC
AGCAAAGTGAGAGACCTTTATGCCCAATTCAAGGGAATTGAAAAATTATATGAATGGCAA
CATACTTGTTTAACATTGAATTCTGTGCAAGAAAGAAAAAATTTAATATATTCCTTGCCA
ACAAGTGGTGGAAAAACCCTCGTGGCTGAGATTTTAATGCTGCAAGAACTGCTTTGCTGT
CGGAAAGATGTTTTAATGATTCTTCCATATGTGGCAATTGTCCAAGAAAAGATTTCAGGT
TTGTCAAGTTTTGGTATAGAACTCGGTTTCTTTGTTGAAGAATATGCTGGAAGCAAAGGA
AGATTTCCTCCAACTAAAAGAAGGGAAAAGAAATCACTCTATATTGCCACTATTGAAAAA
GGACATAGCTTGGTGAACTCCTTGATTGAAACTGGAAGAATTGACAGTCTGGGTCTGGTT
GTTGTAGACGAGTTGCACATGATTGGTGAAGGAAGCCGTGGAGCTACACTGGAAATGACC
CTAGCAAAAATCCTCTACACTAGCAAAACGACTCAAATTATTGGTATGAGTGCAACATTA
AACAATGTTGAAGACCTACAAAAGTTTCTTCAAGCAGAATATTATACCAGTCAATTTAGA
CCAGTTGAGTTAAAAGAATATCTGAAAATAAATGATACAATATATGAAGTTGACAGCAAA
GCTGAGAATGGCATGACTTTTTCACGTCTTCTTAATTATAAGTATTCTGATACCCTAAAA
AAGATGGATCCTGATCACTTGGTAGCATTGGTGACAGAAGTTATTCCCAATTATTCCTGC
TTAGTTTTTTGTCCTAGTAAGAAGAACTGTGAAAATGTAGCAGAAATGATATGCAAATTT
TTAAGCAAGGAATATCTGAAACATAAGGAGAAAGAAAAATGTGAGGTGATTAAGAACTTG
AAGAATATTGGCAATGGCAACCTGTGTCCTGTTTTAAAGCGCACTATCCCATTTGGAGTT
GCCTATCACCACAGTGGCTTAACAAGTGATGAAAGGAAACTCTTGGAGGAGGCCTACTCC
ACAGGAGTGCTCTGTCTTTTTACCTGCACATCTACCCTAGCGGCAGGTGTCAACCTACCA
GCTCGAAGAGTTATTTTAAGAGCTCCCTATGTTGCTAAGGAATTTTTAAAGAGGAATCAA
TATAAACAGATGATTGGCAGAGCTGGTCGTGCTGGAATAGATACTATTGGGGAGAGTATC
CTCATATTGCAAGAAAAAGACAAACAACAGGTATTGGAGTTAATAACTAAACCATTGGAA
AACTGTTACAGCCATCTTGTTCAGGAATTCACCAAGGGAATCCAAACATTATTTCTCTCT
TTGATTGGTTTGAAGATTGCAACGAATCTTGATGACATCTATCATTTCATGAATGGTACA
TTTTTTGGTGTTCAGCAAAAGGTTTTATTGAAAGAAAAAAGTCTCTGGGAAATAACTGTT
GAATCACTTAGATACCTGACAGAAAAAGGACTCCTACAAAAAGACACTATTTATAAGTCT
GAAGAAGAGGTCCAATATAATTTTCATATTACAAAGTTGGGACGTGCTTCATTTAAGGGA
ACTATAGATTTAGCTTATTGTGACATTCTGTACAGAGACTTGAAGAAAGGTCTTGAAGGA
CTTGTGCTTGAAAGCCTTCTTCATCTAATCTACCTAACAACCCCCTATGATCTGGTTTCA
CAGTGTAACCCTGATTGGATGATATACTTCAGGCAGTTTAGCCAACTCAGTCCAGCAGAA
CAAAATGTAGCTGCCATTCTTGGAGTCTCTGAAAGCTTTATTGGGAAGAAAGCATCAGGC
CAAGCCATCGGAAAGAAGGTGGACAAGAACGTTGTCAACAGGCTATATCTGTCTTTTGTT
CTTTATACCTTGCTCAAAGAGACCAACATTTGGACTGTATCTGAAAAATTTAATATGCCT
CGAGGATATATACAAAATCTTCTCACTGGAACTGCCTCATTCTCATCTTGTGTGTTACAT
TTCTGTGAGGAGCTTGAGGAGTTTTGGGTTTACAGAGCCCTTTTGGTAGAACTTACCAAG
AAGCTGACTTACTGTGTAAAGGCAGAATTAATCCCTCTCATGGAAGTTACTGGAGTTTTA
GAGGGTCGAGCAAAACAGTTATACAGTGCAGGTTACAAAAGTCTAATGCACTTAGCTAAT
GCAAATCCTGAAGTGCTCGTAAGGACAATTGATCATTTATCAAGACGCCAAGCCAAGCAA
ATTGTTTCATCAGCAAAGATGCTGTTGCATGAAAAAGCAGAAGCCCTGCAAGAAGAGGTA
GAAGAGTTACTAAGATTGCCTTCTGATTTCCCTGGTGCTGTGGCTTCTTCCACTGACAAA
GCA
Protein sequence predicted based on nucleotide sequence
MDECGSRIRRRVSLPKRNRPSLGCIFGAPTAAELEPGDEGKEEEEMVAENRRRKTAGVLP VEVQPLLLSDSPECLVLGGGDTNPDLLRHMPTDRGVGDQPNDSEVDMFGDYDSFTENSFI AQVDDLEQKYMQLPEHKKHATDFATENLCSESIKNKLSITTIGNLTELQTDKHTENQSGY EGVTIEPGADLLYDVPSSQAIYFENLQNSSNDLGDHSMKERDWKSSSHNTVNEELPHNCI EQPQQNDESSSKVRTSSDMNRRKSIKDHLKNAMTGNAKAQTPIFSRSKQLKDTLLSEEIN VAKKTVESSSNDLGPFYSLPSKVRDLYAQFKGIEKLYEWQHTCLTLNSVQERKNLIYSLP TSGGKTLVAEILMLQELLCCRKDVLMILPYVAIVQEKISGLSSFGIELGFFVEEYAGSKG RFPPTKRREKKSLYIATIEKGHSLVNSLIETGRIDSLGLVVVDELHMIGEGSRGATLEMT LAKILYTSKTTQIIGMSATLNNVEDLQKFLQAEYYTSQFRPVELKEYLKINDTIYEVDSK AENGMTFSRLLNYKYSDTLKKMDPDHLVALVTEVIPNYSCLVFCPSKKNCENVAEMICKF LSKEYLKHKEKEKCEVIKNLKNIGNGNLCPVLKRTIPFGVAYHHSGLTSDERKLLEEAYS TGVLCLFTCTSTLAAGVNLPARRVILRAPYVAKEFLKRNQYKQMIGRAGRAGIDTIGESI LILQEKDKQQVLELITKPLENCYSHLVQEFTKGIQTLFLSLIGLKIATNLDDIYHFMNGT FFGVQQKVLLKEKSLWEITVESLRYLTEKGLLQKDTIYKSEEEVQYNFHITKLGRASFKG TIDLAYCDILYRDLKKGLEGLVLESLLHLIYLTTPYDLVSQCNPDWMIYFRQFSQLSPAE QNVAAILGVSESFIGKKASGQAIGKKVDKNVVNRLYLSFVLYTLLKETNIWTVSEKFNMP RGYIQNLLTGTASFSSCVLHFCEELEEFWVYRALLVELTKKLTYCVKAELIPLMEVTGVL EGRAKQLYSAGYKSLMHLANANPEVLVRTIDHLSRRQAKQIVSSAKMLLHEKAEALQEEV EELLRLPSDFPGAVASSTDKA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
180
End on reference sequence
3485
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
