Gene:
Gene Symbol:
CNTNAP4
Gene Name:
contactin associated protein-like 4
Original Clone ID:
FLH265908.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081284
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGATCTGTCACGGGAGCTGTCCTCAAGACGCTACTTCTGTTATCTACTCAAAATTGG
AACAGAGTCGAAGCTGGGAATTCCTATGACTGTGATGATCCTCTTGTGTCTGCCTTGCCT
CAGGCATCCTTCAGCAGTTCTTCCGAGCTCTCCAGCAGTCATGGTCCTGGATTTGCAAGG
CTGAATAGAAGAGATGGAGCTGGTGGCTGGTCTCCACTTGTGTCTAACAAATACCAGTGG
TTGCAGATTGACCTTGGAGAGAGAATGGAGGTCACCGCTGTGGCCACTCAAGGGGGATAT
GGTAGCTCCAACTGGGTGACCAGCTACCTCCTGATGTTCAGTGATAGTGGCTGGAACTGG
AAACAATATCGCCAAGAGGACAGCATCTGGGGTTTTTCAGGAAATGCAAATGCAGACAGT
GTTGTGTACTATAGACTCCAGCCTTCTATCAAAGCCAGATTTCTGCGCTTCATCCCTTTG
GAATGGAACCCCAAGGGCAGAATTGGAATGCGAATCGAAGTGTTCGGATGTGCATACAGA
TCAGAAGTGGTTGATCTTGATGGAAAAAGTTCCCTTCTCTACAGATTTGATCAAAAATCC
CTGAGCCCAATAAAAGACATTATTTCTTTGAAATTCAAAACCATGCAGAGTGATGGGATT
CTACTCCACAGGGAAGGGCCAAATGGAGATCACATCACACTGCAATTAAGAAGAGCAAGA
CTCTTTTTACTTATTAATTCAGGTGAAGCTAAACTGCCTTCCACTTCCACCCTGGTCAAT
CTCACCCTGGGCAGCCTGCTAGATGATCAGCATTGGCATTCAGTGCTCATCCAGCGTTTG
GGCAAACAAGTCAACTTCACAGTGGACGAACACAGGCATCATTTCCATGCACGGGGAGAA
TTCAATCTCATGAATCTTGATTATGAGATCAGCTTTGGAGGGATTCCAGCACCTGGAAAA
TCAGTGTCATTCCCACATAGAAATTTTCATGGATGTTTAGAAAATCTCTATTATAATGGA
GTGGATATCATTGATTTGGCCAAGCAGCAAAAACCACAGATCATTGCTATGGGAAATGTG
TCATTTTCTTGTTCACAACCACAATCTATGCCCGTGACTTTTCTGAGCTCCAGGAGTTAT
TTAGCACTGCCAGACTTCTCTGGAGAGGAGGAGGTTTCTGCCACTTTTCAATTTCGAACT
TGGAATAAGGCAGGGCTTCTGCTGTTCAGTGAACTTCAGCTGATTTCAGGGGGTATCCTC
CTCTTTCTGAGTGATGGAAAACTTAAGTCGAATCTCTACCAGCCAGGAAAATTACCCAGT
GACATCACAGCAGGTGTCGAATTAAATGATGGGCAGTGGCATTCTGTCTCTTTATCTGCT
AAAAAGAATCACTTGAGTGTGGCGGTGGACGGCCAGATGGCTTCTGCTGCTCCTCTGCTG
GGGCCTGAGCAGATTTATTCGGGTGGCACCTATTATTTTGGAGGTTGTCCTGACAAAAGC
TTTGGATCCAAATGTAAAAGTCCACTTGGTGGATTTCAGGGATGTATGAGGCTCATTTCT
ATCAGCGGCAAAGTGGTAGATCTGATTTCAGTTCAGCAGGGGTCCCTTGGGAACTTCAGT
GACCTTCAGATAGACTCATGTGGCATCTCAGACAGGTGTTTGCCCAACTATTGTGAACAC
GGTGGGGAGTGTTCCCAGTCCTGGAGCACCTTTCATTGTAACTGTACCAACACTGGTTAC
AGAGGAGCTACTTGCCATAACTCTATCTATGAGCAGTCATGTGAAGCCTATAAGCACAGA
GGAAATACTTCAGGGTTTTACTATATAGATTCAGATGGAAGTGGTCCCCTGGAACCATTT
CTTCTATATTGCAATATGACCGAAACTGCATGGACCATCATACAGCACAACGGCTCTGAC
TTAACAAGAGTCAGAAATACTAATCCAGAGAACCCATATGCTGGGTTTTTCGAGTATGTG
GCCAGCATGGAGCAACTTCAGGCCACTATTAACCGTGCAGAGCACTGTGAACAGGAGTTT
ACTTATTACTGCAAGAAGTCACGGCTGGTCAATAAGCAAGATGGAACCCCTCTGAGTTGG
TGGGTAGGAAGAACCAATGAAACGCAAACCTACTGGGGAGGTTCTTCGCCTGATCTTCAA
AAATGTACTTGTGGATTAGAGGGAAACTGCATTGATTCTCAGTATTACTGCAATTGTGAT
GCTGACCGGAATGAATGGACCAATGACACTGGATTGCTTGCTTATAAAGAACATCTTCCA
GTAACTAAGATCGTGATTACAGACACAGGCCGACTGCATTCAGAAGCAGCTTATAAACTG
GGGCCTCTGCTCTGCCAGGGAGACAGATCATTTTGGAATTCAGCTTCCTTTGATACCGAG
GCTTCATATCTTCATTTTCCTACCTTCCACGGAGAACTTAGCGCGGATGTATCTTTCTTT
TTTAAGACAACAGCTTCATCTGGGGTATTTTTAGAGAACTTGGGGATTGCTGATTTTATA
CGGATAGAGCTTCGCTCTCCGACAGTAGTGACTTTTTCATTTGATGTGGGGAATGGGCCT
TTTGAAATCTCAGTGCAGTCACCCACCCACTTCAACGACAACCAGTGGCACCATGTGAGG
GTTGAAAGGAACATGAAGGAGGCCTCCCTTCAAGTGGATCAGCTGACACCAAAGACACAG
CCCGCCCCCGCTGATGGGCATGTCCTGTTACAGCTCAACAGTCAGCTCTTCGTGGGTGGA
ACGGCCACCAGACAGAGAGGCTTTCTGGGCTGCATTCGGTCTCTGCAGTTGAATGGGATG
ACCCTGGATTTGGAAGAAAGAGCCCAGGTGACTCCAGAAGTGCAGCCAGGTTGTAGGGGA
CATTGCAGCAGCTATGGGAAGTTATGCCGCAATGGAGGGAAATGCAGAGAAAGACCCATT
GGGTTCTTTTGTGACTGCACTTTCTCTGCATACACAGGGCCATTCTGCTCAAATGAGATT
TCTGCATATTTTGGATCTGGCTCATCCGTGATATACAATTTTCAAGAAAATTATCTTTTA
AGTAAAAACTCCAGCTCCCACGCTGCTTCATTTCATGGTGATATGAAGCTGAGCAGAGAA
ATGATCAAATTTAGTTTCCGAACAACACGAACACCAAGCTTGCTGCTTTTTGTGAGCTCC
TTTTACAAAGAATACCTTTCTGTGATCATTGCCAAAAATGGAAGTTTGCAGATCAGGTAC
AAGTTAAATAAATATCAAGAGCCTGATGTTGTTAACTTTGATTTTAAAAACATGGCTGAT
GGACAACTTCACCACATAATGATTAACAGAGAAGAAGGAGTGGTCTTTATAGAGATTGAC
GATAATAGAAGGAGACAAGTTCACCTGTCATCAGGCACAGAATTCAGTGCAGTCAAATCT
CTGGTATTGGGCAGGATTTTAGAACACAGTGATGTGGACCAGGATACTGCACTGGCAGGT
GCGCAGGGCTTCACAGGCTGCCTCTCTGCAGTGCAGCTCAGCCACGTGGCCCCTCTGAAG
GCAGCTCTGCACCCCAGCCACCCAGACCCTGTCACTGTTACAGGACACGTGACTGAGTCC
AGCTGTATGGCCCAGCCTGGCACTGATGCCACATCAAGGGAAAGGACACACTCGTTTGCA
GATCATTCTGGAACAATAGATGACAGAGAGCCCCTTGCTAATGCAATCAAAAGTGACTCT
GCAGTAATTGGAGGTCTGATAGCTGTTGTGATTTTTATCTTGCTTTGCATCACTGCCATA
GCTGTTCGCATTTATCAGCAGAAAAGGTTATATAAAAGAAGTGAGGCAAAAAGGTCAGAG
AATGTAGACAGTGCTGAGGCTGTTCTGAAAAGTGAGCTTAATATACAAAATGCAGTCAAT
GAAAATCAGAAAGAGTACTTCTTC
Protein sequence predicted based on nucleotide sequence
MGSVTGAVLKTLLLLSTQNWNRVEAGNSYDCDDPLVSALPQASFSSSSELSSSHGPGFAR LNRRDGAGGWSPLVSNKYQWLQIDLGERMEVTAVATQGGYGSSNWVTSYLLMFSDSGWNW KQYRQEDSIWGFSGNANADSVVYYRLQPSIKARFLRFIPLEWNPKGRIGMRIEVFGCAYR SEVVDLDGKSSLLYRFDQKSLSPIKDIISLKFKTMQSDGILLHREGPNGDHITLQLRRAR LFLLINSGEAKLPSTSTLVNLTLGSLLDDQHWHSVLIQRLGKQVNFTVDEHRHHFHARGE FNLMNLDYEISFGGIPAPGKSVSFPHRNFHGCLENLYYNGVDIIDLAKQQKPQIIAMGNV SFSCSQPQSMPVTFLSSRSYLALPDFSGEEEVSATFQFRTWNKAGLLLFSELQLISGGIL LFLSDGKLKSNLYQPGKLPSDITAGVELNDGQWHSVSLSAKKNHLSVAVDGQMASAAPLL GPEQIYSGGTYYFGGCPDKSFGSKCKSPLGGFQGCMRLISISGKVVDLISVQQGSLGNFS DLQIDSCGISDRCLPNYCEHGGECSQSWSTFHCNCTNTGYRGATCHNSIYEQSCEAYKHR GNTSGFYYIDSDGSGPLEPFLLYCNMTETAWTIIQHNGSDLTRVRNTNPENPYAGFFEYV ASMEQLQATINRAEHCEQEFTYYCKKSRLVNKQDGTPLSWWVGRTNETQTYWGGSSPDLQ KCTCGLEGNCIDSQYYCNCDADRNEWTNDTGLLAYKEHLPVTKIVITDTGRLHSEAAYKL GPLLCQGDRSFWNSASFDTEASYLHFPTFHGELSADVSFFFKTTASSGVFLENLGIADFI RIELRSPTVVTFSFDVGNGPFEISVQSPTHFNDNQWHHVRVERNMKEASLQVDQLTPKTQ PAPADGHVLLQLNSQLFVGGTATRQRGFLGCIRSLQLNGMTLDLEERAQVTPEVQPGCRG HCSSYGKLCRNGGKCRERPIGFFCDCTFSAYTGPFCSNEISAYFGSGSSVIYNFQENYLL SKNSSSHAASFHGDMKLSREMIKFSFRTTRTPSLLLFVSSFYKEYLSVIIAKNGSLQIRY KLNKYQEPDVVNFDFKNMADGQLHHIMINREEGVVFIEIDDNRRRQVHLSSGTEFSAVKS LVLGRILEHSDVDQDTALAGAQGFTGCLSAVQLSHVAPLKAALHPSHPDPVTVTGHVTES SCMAQPGTDATSRERTHSFADHSGTIDDREPLANAIKSDSAVIGGLIAVVIFILLCITAI AVRIYQQKRLYKRSEAKRSENVDSAEAVLKSELNIQNAVNENQKEYFF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
386
End on reference sequence
4312
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
