Gene:
Gene Symbol:
EVI5
Gene Name:
ecotropic viral integration site 5
Original Clone ID:
FLH265910.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 26433934
Title: A non-synonymous single-nucleotide polymorphism associated with multiple sclerosis risk affects the EVI5 interactome
Title: A non-synonymous single-nucleotide polymorphism associated with multiple sclerosis risk affects the EVI5 interactome
Authors:
The ORFeome Collaboration
HsCD00081286
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGTTACCAACAAAATGACTGCTGCCTTTAGAAACCCTAGTGGGAAACAGGTGGCGACA
GACAAAGTTGCAGAAAAGCTGAGCTCTACTCTCTCATGGGTGAAGAACACAGTATCGCAT
ACAGTCAGTCAGATGGCCAGTCAGGTGGCAAGTCCATCTACTTCATTACATACCACATCC
TCATCTACCACACTATCAACACCAGCCCTTTCACCATCTTCCCCATCACAGTTGAGTCCA
GACGACTTAGAACTCCTGGCTAAACTGGAAGAACAGAATAGATTGTTAGAAACGGATAGT
AAGTCTTTAAGATCTGTAAATGGGTCAAGAAGAAACAGTGGCTCTTCTCTTGTGTCGAGT
TCATCAGCCTCTAGCAACCTCAGTCACCTTGAAGAAGATTCTTGGATTCTTTGGGGAAGA
ATTGTTAATGAATGGGAAGATGTACGCAAAAAGAAGGAAAAGCAAGTTAAGGAACTTGTT
CATAAAGGGATACCCCATCACTTTAGAGCAATAGTTTGGCAACTTTTATGCAGTGCACAA
AGTATGCCAATTAAGGATCAGTATTCAGAACTCCTGAAAATGACCTCGCCTTGTGAAAAA
TTGATCCGAAGGGACATTGCTAGAACTTACCCTGAACACAACTTTTTTAAGGAAAAAGAT
AGCCTTGGACAGGAGGTTTTATTTAATGTAATGAAGGCTTACTCTTTAGTAGATCGTGAG
GTTGGTTACTGTCAAGGAAGTGCTTTTATAGTTGGATTGTTGCTTATGCAGATGCCAGAA
GAAGAAGCTTTCTGTGTATTTGTTAAATTAATGCAAGATTATAGACTTCGTGAACTTTTT
AAACCAAGTATGGCAGAATTGGGCCTTTGTATGTACCAGTTTGAATGTATGATACAGGAG
CATCTTCCAGAGCTCTTTGTACATTTTCAATCTCAGAGTTTTCATACCTCAATGTATGCA
TCATCCTGGTTTCTGACTATCTTTCTTACAACTTTTCCACTACCAATTGCAACAAGGATA
TTTGATATCTTTATGTCTGAGGGTTTAGAAATAGTGTTTCGTGTAGGATTAGCACTTCTT
CAGATGAATCAGGCAGAACTGATGCAACTTGACATGGAAGGGATGTTACAGCACTTTCAA
AAGGTCATTCCACATCAGTTTGATGGTGTCCCAGACAAGCTAATCCAAGCAGCTTACCAA
GTCAAATACAATTCAAAAAAAATGAAAAAGCTTGAAAAGGAATACACTACAATAAAAACG
AAAGAAATGGAAGAGCAAGTTGAAATTAAAAGGTTACGCACAGAAAATAGACTTTTAAAA
CAGCGCATCGAGACATTAGAAAAACATAAATGCAGTTCCAACTACAACGAAGATTTTGTG
CTACAGCTAGAGAAGGAATTGGTCCAAGCCCGACTGAGTGAAGCTGAGTCTCAGTGTGCA
TTAAAAGAGATGCAGGATAAAGTCTTGGATATAGAGAAGAGGAATAACTCCCTTCCTGAT
GAGAATAATATTGCAAGGCTTCAGGAAGAACTCATTGCTGTGAAACTTAGAGAAGCAGAA
GCCATTATGGGTTTGAAAGAACTTAGACAGCAAGTCAAGGATTTAGAGGAACACTGGCAG
CGCCACTTAGCTCGTACTACTGGGAGATGGAAAGACCCACCCAAGAAAAATGCTATGAAT
GAGTTACAAGATGAACTGATGACCATTCGACTTAGAGAAGCTGAAACACAAGCAGAAATA
AGAGAAATAAAACAAAGGATGATGGAAATGGAAACACAGAACCAGATCAATAGTAACCAT
CTTCGAAGAGCAGAACAAGAGGTGATTAGCCTACAGGAGAAAGTGCAGTATCTTTCTGCA
CAGAACAAAGGACTCCTTACTCAATTAAGTGAAGCAAAGCGTAAACAAGCAGAGATTGAA
TGCAAGAATAAGGAAGAAGTGATGGCTGTGAGGCTTCGGGAAGCAGATAGCATAGCTGCT
GTGGCTGAACTACGACAACACATTGCTGAGCTTGAAATCCAGAAAGAAGAAGGAAAGCTT
CAAGGACAGCTTAACAAGTCTGATTCTAACCAGTATATTGGGGAACTGAAAGATCAGATA
GCAGAGCTGAATCATGAGCTCAGGTGCCTAAAAGGCCAGAGGGGCTTCTCAGGCCAACCT
CCTTTTGATGGAATCCACATTGTCAACCATTTAATAGGAGATGATGAATCATTCCATTCC
TCCGATGAAGATTTTATAGATAATTCCTTACAGGAAACTGGTGTTGGTTTTCCTTTGCAC
GGAAAATCTGGTTCGATGTCTTTGGACCCCGCAGTGGCAGATGGTAGTGAGAGCGAAACA
GAAGACAGTGTGCTGGAGACCAGAGAGAGCAACCAAGTGGTTCAAAAGGAGCGGCCCCCG
AGAAGAAGAGAGTCGTATTCAACCACTGTC
Protein sequence predicted based on nucleotide sequence
MVTNKMTAAFRNPSGKQVATDKVAEKLSSTLSWVKNTVSHTVSQMASQVASPSTSLHTTS SSTTLSTPALSPSSPSQLSPDDLELLAKLEEQNRLLETDSKSLRSVNGSRRNSGSSLVSS SSASSNLSHLEEDSWILWGRIVNEWEDVRKKKEKQVKELVHKGIPHHFRAIVWQLLCSAQ SMPIKDQYSELLKMTSPCEKLIRRDIARTYPEHNFFKEKDSLGQEVLFNVMKAYSLVDRE VGYCQGSAFIVGLLLMQMPEEEAFCVFVKLMQDYRLRELFKPSMAELGLCMYQFECMIQE HLPELFVHFQSQSFHTSMYASSWFLTIFLTTFPLPIATRIFDIFMSEGLEIVFRVGLALL QMNQAELMQLDMEGMLQHFQKVIPHQFDGVPDKLIQAAYQVKYNSKKMKKLEKEYTTIKT KEMEEQVEIKRLRTENRLLKQRIETLEKHKCSSNYNEDFVLQLEKELVQARLSEAESQCA LKEMQDKVLDIEKRNNSLPDENNIARLQEELIAVKLREAEAIMGLKELRQQVKDLEEHWQ RHLARTTGRWKDPPKKNAMNELQDELMTIRLREAETQAEIREIKQRMMEMETQNQINSNH LRRAEQEVISLQEKVQYLSAQNKGLLTQLSEAKRKQAEIECKNKEEVMAVRLREADSIAA VAELRQHIAELEIQKEEGKLQGQLNKSDSNQYIGELKDQIAELNHELRCLKGQRGFSGQP PFDGIHIVNHLIGDDESFHSSDEDFIDNSLQETGVGFPLHGKSGSMSLDPAVADGSESET EDSVLETRESNQVVQKERPPRRRESYSTTV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
11
End on reference sequence
2443
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
