Gene:
Gene Symbol:
SPINK5
Gene Name:
serine peptidase inhibitor, Kazal type 5
Original Clone ID:
FLH265914.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081290
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAAGATAGCCACAGTGTCAGTGCTTCTGCCCTTGGCTCTTTGCCTCATACAAGATGCT
GCCAGTAAGAATGAAGATCAGGAAATGTGCCATGAATTTCAGGCATTTATGAAAAATGGA
AAACTGTTCTGTCCCCAGGATAAGAAATTTTTTCAAAGTCTTGATGGAATAATGTTCATC
AATAAATGTGCCACGTGCAAAATGATACTGGAAAAAGAAGCAAAATCACAGAAGAGGGCC
AGGCATTTAGCAAGAGCTCCCAAGGCTACTGCCCCAACAGAGCTGAATTGTGATGATTTT
AAAAAAGGAGAAAGAGATGGGGATTTTATCTGTCCTGATTATTATGAAGCTGTTTGTGGC
ACAGATGGGAAAACATATGACAACAGATGTGCACTGTGTGCTGAGAATGCGAAAACCGGG
TCCCAAATTGGTGTAAAAAGTGAAGGGGAATGTAAGAGCAGTAATCCAGAGCAGGATGTA
TGCAGTGCTTTTCGGCCCTTTGTTAGAGATGGAAGACTTGGATGCACAAGGGAAAATGAT
CCTGTTCTTGGTCCTGATGGGAAGACGCATGGCAATAAGTGTGCAATGTGTGCTGAGCTG
TTTTTAAAAGAAGCTGAAAATGCCAAGCGAGAGGGTGAAACTAGAATTCGACGAAATGCT
GAAAAGGATTTTTGCAAGGAATATGAAAAACAAGTGAGAAATGGAAGGCTTTTTTGTACA
CGGGAGAGTGATCCAGTCCGTGGCCCTGACGGCAGGATGCATGGCAACAAATGTGCCCTG
TGTGCTGAAATTTTCAAGCAGCGTTTTTCAGAGGAAAACAGTAAAACAGATCAAAATTTG
GGAAAAGCTGAAGAAAAAACTAAAGTTAAAAGAGAAATTGTGAAACTCTGCAGTCAATAT
CAAAATCAGGCAAAGAATGGAATACTTTTCTGTACCAGAGAAAATGACCCTATTCGTGGT
CCAGATGGGAAAATGCATGGCAACTTGTGTTCCATGTGTCAAGCCTACTTCCAAGCAGAA
AATGAAGAAAAGAAAAAGGCTGAAGCACGAGCTAGAAACAAAAGAGAATCTGGAAAAGCA
ACCTCATATGCAGAGCTTTGCAGTGAATATCGAAAGCTTGTGAGGAACGGAAAACTTGCT
TGCACCAGAGAGAACGATCCTATCCAGGGCCCAGATGGGAAAGTGCATGGCAACACCTGC
TCCATGTGTGAGGTCTTCTTCCAAGCAGAAGAAGAAGAAAAGAAAAAGAAGGAAGGTAAA
TCAAGAAACAAAAGACAATCTAAGAGTACAGCTTCCTTTGAGGAGTTGTGTAGTGAATAC
CGCAAATCCAGGAAAAACGGACGGCTTTTTTGCACCAGAGAGAATGACCCCATCCAGGGC
CCAGATGGAAAAATGCATGGCAACACCTGCTCCATGTGTGAGGCCTTCTTTCAACAAGAA
GAAAGAGCAAGAGCAAAGGCTAAAAGAGAAGCTGCAAAGGAAATCTGCAGTGAATTTCGG
GACCAAGTGAGGAATGGAACACTTATATGCACCAGGGAGCATAATCCTGTCCGTGGCCCA
GATGGCAAAATGCATGGAAACAAGTGTGCCATGTGTGCCAGTGTGTTCAAACTTGAAGAA
GAAGAGAAGAAAAATGATAAAGAAGAAAAAGGGAAAGTCGAGGCTGAAAAAGTTAAGAGA
GAAGCAGTTCAGGAGCTGTGCAGTGAATATCGTCATTATGTGAGGAATGGACGACTCCCC
TGTACCAGAGAGAATGATCCTATTGAGGGTCTAGATGGGAAAATCCACGGCAACACCTGC
TCCATGTGTGAAGCCTTCTTCCAGCAAGAAGCAAAAGAAAAAGAAAGAGCTGAACCCAGA
GCAAAAGTCAAAAGAGAAGCTGAAAAGGAGACATGCGATGAATTTCGGAGACTTTTGCAA
AATGGAAAACTTTTCTGCACAAGAGAAAATGATCCTGTGCGTGGCCCAGATGGCAAGACC
CATGGCAACAAGTGTGCCATGTGTAAGGCAGTCTTCCAGAAAGAAAATGAGGAAAGAAAG
AGGAAAGAAGAGGAAGATCAGAGAAATGCTGCAGGACATGGTTCCAGTGGTGGTGGAGGA
GGAAACACTCAGGACGAATGTGCTGAGTATCGGGAACAAATGAAAAATGGAAGACTCAGC
TGTACTCGGGAGAGTGATCCTGTACGTGATGCTGATGGCAAATCGTACAACAATCAGTGT
ACCATGTGTAAAGCAAAATTGGAAAGAGAAGCAGAGAGAAAAAATGAGTATTCTCGCTCC
AGATCAAATGGGACTGGATCAGAATCAGGGAAGGATACATGTGATGAGTTTAGAAGCCAA
ATGAAAAATGGAAAACTCATCTGCACTCGAGAAAGTGACCCTGTCCGGGGTCCAGATGGC
AAGACACATGGCAATAAGTGTACTATGTGTAAGGAAAAACTGGAAAGGGAAGCAGCTGAA
AAAAAAAAGAAAGAGGATGAAGACAGGAGCAATACAGGAGAAAGGAGCAATACAGGAGAA
AGGAGCAATGACAAAGAGGATCTGTGTCGTGAATTTCGAAGCATGCAGAGAAATGGAAAG
CTTATCTGCACCAGAGAAAATAACCCTGTTCGAGGCCCATATGGCAAGATGCACATCAAT
AAATGTGCTATGTGTCAGAGCATCTTTGATCGAGAAGCTAATGAAAGAAAAAAGAAAGAT
GAAGAGAAATCAAGTAGCAAGCCCTCAAATAATGCAAAGGATGAGTGCAGTGAATTTCGA
AACTATATAAGGAACAATGAACTCATCTGCCCTAGAGAGAATGACCCAGTGCACGGTGCT
GATGGAAAGTTCTATACAAACAAGTGCTACATGTGCAGAGCTGTCTTTCTAACAGAAGCT
TTGGAAAGGGCAAAGCTTCAAGAAAAGCCATCCCATGTTAGAGCTTCTCAAGAGGAAGAC
AGCCCAGACTCTTTCAGTTCTCTGGATTCTGAGATGTGCAAAGACTACCGAGTATTGCCC
AGGATAGGTTATCTTTGTCCAAAGGATTTAAAGCCTGTCTGTGGTGACGATGGCCAAACC
TACAACAATCCTTGCATGCTCTGTCATGAAAACCTGATACGCCAAACAAATACACACATC
CGCAGTACAGGGAAGTGTGAGGAGAGCAGCACCCCAGGAACCACCGCAGCCAGCATGCCC
CCGTCTGACGAA
Protein sequence predicted based on nucleotide sequence
MKIATVSVLLPLALCLIQDAASKNEDQEMCHEFQAFMKNGKLFCPQDKKFFQSLDGIMFI NKCATCKMILEKEAKSQKRARHLARAPKATAPTELNCDDFKKGERDGDFICPDYYEAVCG TDGKTYDNRCALCAENAKTGSQIGVKSEGECKSSNPEQDVCSAFRPFVRDGRLGCTREND PVLGPDGKTHGNKCAMCAELFLKEAENAKREGETRIRRNAEKDFCKEYEKQVRNGRLFCT RESDPVRGPDGRMHGNKCALCAEIFKQRFSEENSKTDQNLGKAEEKTKVKREIVKLCSQY QNQAKNGILFCTRENDPIRGPDGKMHGNLCSMCQAYFQAENEEKKKAEARARNKRESGKA TSYAELCSEYRKLVRNGKLACTRENDPIQGPDGKVHGNTCSMCEVFFQAEEEEKKKKEGK SRNKRQSKSTASFEELCSEYRKSRKNGRLFCTRENDPIQGPDGKMHGNTCSMCEAFFQQE ERARAKAKREAAKEICSEFRDQVRNGTLICTREHNPVRGPDGKMHGNKCAMCASVFKLEE EEKKNDKEEKGKVEAEKVKREAVQELCSEYRHYVRNGRLPCTRENDPIEGLDGKIHGNTC SMCEAFFQQEAKEKERAEPRAKVKREAEKETCDEFRRLLQNGKLFCTRENDPVRGPDGKT HGNKCAMCKAVFQKENEERKRKEEEDQRNAAGHGSSGGGGGNTQDECAEYREQMKNGRLS CTRESDPVRDADGKSYNNQCTMCKAKLEREAERKNEYSRSRSNGTGSESGKDTCDEFRSQ MKNGKLICTRESDPVRGPDGKTHGNKCTMCKEKLEREAAEKKKKEDEDRSNTGERSNTGE RSNDKEDLCREFRSMQRNGKLICTRENNPVRGPYGKMHINKCAMCQSIFDREANERKKKD EEKSSSKPSNNAKDECSEFRNYIRNNELICPRENDPVHGADGKFYTNKCYMCRAVFLTEA LERAKLQEKPSHVRASQEEDSPDSFSSLDSEMCKDYRVLPRIGYLCPKDLKPVCGDDGQT YNNPCMLCHENLIRQTNTHIRSTGKCEESSTPGTTAASMPPSDE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3237
Start on reference sequence
43
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
