Gene:
Gene Symbol:
XRN1
Gene Name:
5'-3' exoribonuclease 1
Original Clone ID:
FLH265924.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081300
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGAGTCCCCAAGTTTTACAGATGGATCTCAGAGCGGTATCCCTGTCTCAGCGAAGTG
GTGAAAGAGCATCAGATTCCTGAATTTGACAACTTGTACCTGGATATGAATGGAATTATA
CATCAGTGCTCCCATCCTAATGATGATGATGTTCACTTTAGAATTTCAGATGATAAAATC
TTTACTGATATTTTTCACTACCTGGAGGTGTTGTTTCGCATTATTAAACCCAGGAAAGTG
TTCTTTATGGCTGTAGATGGTGTGGCTCCTCGAGCAAAAATGAACCAGCAGCGTGGGAGG
CGTTTTAGGTCAGCAAAGGAGGCAGAAGACAAAATTAAAAAGGCAATAGAGAAGGGAGAA
ACTCTTCCTACAGAGGCCAGATTTGATTCCAACTGTATCACACCAGGAACTGAATTTATG
GCCAGGTTACATGAACATCTGAAGTATTTTGTAAATATGAAAATTTCCACAGACAAGTCA
TGGCAAGGAGTTACCATCTACTTCTCAGGCCATGAGACTCCTGGAGAAGGAGAGCATAAA
ATCATGGAATTTATCAGATCCGAGAAAGCAAAGCCAGATCATGATCCAAACACCAGACAC
TGTCTTTATGGTTTAGATGCTGACTTGATTATGCTTGGATTAACAAGTCATGAGGCACAT
TTTTCTCTCTTAAGAGAAGAAGTTCGATTTGGTGGCAAAAAAACACAACGGGTATGTGCT
CCAGAAGAAACTACATTTCACCTTCTACACTTGTCTTTAATGAGAGAGTATATTGACTAT
GAGTTTTCAGTATTAAAAGAAAAGATCACATTTAAATATGATATTGAAAGGATAATAGAT
GATTGGATTTTGATGGGGTTTCTTGTTGGTAATGATTTTATCCCTCATCTACCTCATTTA
CATATTAATCATGATGCACTGCCTCTTCTTTATGGAACATATGTTACCATCCTGCCAGAA
CTTGGGGGTTATATTAATGAAAGTGGGCACCTCAACTTACCTCGATTTGAGAAATACCTT
GTGAAACTATCAGATTTTGATCGGGAGCACTTCAGTGAAGTTTTTGTGGACCTAAAATGG
TTTGAAAGCAAAGTTGGTAACAAGTACCTCAATGAAGCAGCAGGTGTCGCAGCAGAAGAA
GCCAGGAACTACAAGGAAAAGAAAAAGTTAAAGGGCCAGGAAAATTCTCTGTGTTGGACT
GCTTTAGACAAAAATGAAGGCGAAATGATAACTTCTAAGGATAATTTAGAAGATGAGACT
GAAGATGATGACCTATTTGAAACTGAGTTTAGACAATATAAAAGAACATATTACATGACG
AAGATGGGGGTTGACGTAGTATCTGATGACTTTCTGGCTGATCAAGCTGCATGTTATGTT
CAGGCAATACAGTGGATTTTGCACTATTACTATCATGGAGTTCAGTCCTGGAGCTGGTAT
TATCCTTATCATTATGCACCTTTCCTGTCTGATATACACAACATCAGTACACTCAAAATC
CATTTTGAACTAGGAAAACCTTTTAAGCCATTTGAACAGCTTCTTGCTGTACTTCCAGCA
GCCAGCAAAAATTTACTTCCTGCATGCTACCAGCATTTGATGACCAATGAAGACTCACCA
ATTATAGAATATTACCCACCTGATTTTAAAACTGACCTAAATGGGAAACAACAGGAATGG
GAAGCTGTGGTGTTAATCCCTTTTATTGATGAGAAGCGATTATTGGAAGCCATGGAGACA
TGTAACCACTCCCTCAAAAAGGAAGAGAGGAAAAGAAACCAACATAGTGAGTGCCTAATG
TGCTGGTATGATAGAGACACAGAGTTTATCTATCCTTCTCCATGGCCAGAAAAGTTCCCT
GCCATAGAACGATGTTGTACAAGGTATAAAATAATATCCTTAGATGCTTGGCGTGTAGAC
ATAAACAAAAACAAAATAACCAGAATTGACCAGAAAGCATTATATTTCTGTGGATTTCCT
ACTCTGAAACACATCAGACACAAATTTTTTTTGAAGAAAAGTGGTGTTCAAGTATTCCAG
CAAAGCAGTCGTGGAGAAAACATGATGTTGGAAATCTTAGTGGATGCAGAATCAGATGAA
CTTACCGTAGAAAATGTAGCTTCATCAGTGCTTGGAAAATCTGTCTTTGTTAATTGGCCT
CACCTTGAGGAAGCTAGAGTCGTGGCTGTATCAGATGGAGAAACTAAGTTTTACTTGGAA
GAACCTCCAGGAACACAGAAGCTTTATTCAGGAAGAACTGCCCCACCATCTAAAGTGGTT
CATCTTGGAGATAAAGAACAATCTAACTGGGCAAAAGAAGTACAAGGAATTTCAGAACAC
TACCTGAGAAGAAAAGGAATAATAATAAATGAAACATCTGCAGTTGTGTATGCTCAGTTA
CTCACAGGTCGTAAATATCAAATAAATCAAAATGGTGAAGTTCGTCTAGAGAAACAGTGG
TCAAAACAAGTTGTTCCTTTTGTTTATCAAACTATTGTCAAGGACATCCGAGCTTTCGAC
TCCCGTTTCTCCAATATCAAAACATTGGATGATTTGTTTCCTCTGAGAAGTATGGTCTTT
ATGCTGGGAACTCCCTATTATGGCTGCACTGGAGAAGTTCAGGATTCAGGTGATGTGATT
ACAGAAGGTAGGATTCGTGTGATTTTCAGCATTCCATGTGAACCCAATCTTGATGCTTTA
ATACAGAACCAGCATAAATATTCTATAAAGTACAACCCAGGATATGTGTTGGCCAGTCGC
CTTGGAGTGAGTGGATACCTTGTTTCAAGGTTTACAGGAAGTATTTTTATTGGAAGAGGA
TCTAGGAGAAACCCTCATGGAGACCATAAAGCAAATGTGGGTTTAAATCTCAAATTCAAC
AAGAAAAATGAGGAGGTACCTGGATATACTAAGAAAGTTGGAAGTGAATGGATGTATTCA
TCTGCAGCAGAACAACTTCTGGCAGAGTACTTAGAGAGAGCTCCAGAACTATTTAGTTAT
ATAGCCAAAAATAGCCAAGAGGATGTGTTCTATGAAGATGACATTTGGCCTGGAGAAAAT
GAGAATGGTGCTGAAAAAGTTCAAGAAATTATTACTTGGCTAAAAGGACATCCTGTCAGT
ACTTTATCTCGTTCTTCTTGTGATTTACAAATTCTGGATGCAGCTATTGTTGAGAAAATT
GAGGAAGAAGTCGAAAAGTGCAAGCAAAGAAAGAATAATAAGAAGGTGCGAGTAACAGTG
AAACCCCATTTGCTATACAGACCTTTAGAACAGCAACATGGAGTCATTCCTGATCGGGAT
GCAGAATTTTGTCTTTTTGACCGTGTTGTAAATGTGAGAGAAAACTTCTCAGTTCCAGTT
GGCCTTCGAGGCACCATCATAGGAATAAAAGGAGCTAATAGAGAAGCCGATGTACTATTT
GAAGTATTATTTGATGAAGAATTTCCTGGAGGGTTAACAATAAGATGCTCACCTGGTAGA
GGTTATCGACTGCCAACAAGTGCCTTGGTGAACCTTTCTCATGGGAGTCGCTCTGAAACT
GGAAATCAGAAGTTGACAGCCATCGTAAAACCACAACCAGCTGTACATCAACATAGCTCA
AGTTCATCAGTTTCCTCTGGGCATTTGGGAGCCCTCAACCATTCCCCTCAATCACTTTTT
GTTCCTACTCAAGTACCTACTAAAGATGATGATGAATTCTGCAACATTTGGCAGTCCTTA
CAGGGATCTGGAAAGATGCAATACTTTCAGCCAACTATACAAGAGAAGGGTGCAGTTCTA
CCTCAAGAAATAAGCCAAGTAAATCAACATCATAAATCTGGCTTTAATGACAACAGTGTT
AAATATCAGCAAAGAAAACATGACCCTCACAGAAAATTTAAAGAAGAGTGTAAGAGTCCT
AAAGCTGAGTGTTGGTCCCAAAAAATGTCCAATAAGCAGCCTAACTCTGGAATTGAGAAC
TTTTTAGCATCTTTGAATATCTCCAAAGAAAATGAAGTACAGTCATCTCATCATGGGGAG
CCTCCAAGTGAAGAGCATTTGTCACCACAGTCATTTGCCATGGGAACACGGATGCTTAAA
GAAATTCTAAAAATTGATGGCTCTAACACTGTGGACCATAAGAATGAAATCAAACAGATT
GCTAATGAAATCCCTGTTTCCTCTAACAGAAGAGATGAATATGGATTACCCTCTCAGCCT
AAACAAAATAAGAAATTAGCATCTTATATGAACAAGCCTCACAGTGCTAATGAGTACCAT
AATGTTCAGTCTATGGACAATATGTGTTGGCCTGCCCCCAGCCAGATCCCTCCTGTATCC
ACACCAGTAACTGAACTTTCTCGAATTTGTTCCCTTGTTGGAATGCCACAACCTGATTTC
TCCTTTCTTAGGATGCCACAGACAATGACCGTTTGCCAAGTAAAATTATCTAATGGCTTA
CTGGTACATGGGCCACAGTGCCACTCTGAAAATGAAGCCAAAGAGAAAGCTGCACTTTTT
GCTTTACAACAGTTGGGCTCCTTAGGCATGAATTTCCCTTTGCCTTCACAAGTATTTGCA
AATTATCCTTCAGCTGTACCACCTGGAACCATTCCTCCAGCCTTTCCCCCACCTACTGGC
TGGGATCACTATGGAAGCAACTATGCATTGGGGGCAGCTAATATAATGCCTTCGTCGTCT
CATCTCTTTGGCTCAATGCCATGGGGACCATCGGTGCCAGTTCCTGGGAAGCCCTTCCAT
CATACTTTATATTCTGGGACCATGCCCATGGCTGGGGGAATACCAGGGGGTGTGCACAAT
CAGTTTATACCTCTGCAGGTTACTAAAAAAAGGGTTGCAAACAAAAAGAACTTTGAGAAT
AAGGAAGCCCAGAGTTCTCAAGCCACTCCAGTTCAGACTAGCCAGCCAGATTCTTCCAAC
ATTGTCAAAGTAAGTCCACGGGAGAGCTCATCAGCTTCTTTGAAGTCCTCTCCGATTGCT
CAACCTGCATCTTCTTTTCAAGTTGAAACTGCCTCTCAAGGCCATAGTATATCTCACCAT
AAGTCAACACCAATCTCTTCTTCAAGAAGAAAATCAAGAAAACTGGCTGTTAATTTTGGT
GTTTCTAAACCTTCTGAG
Protein sequence predicted based on nucleotide sequence
MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKI FTDIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGE TLPTEARFDSNCITPGTEFMARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHK IMEFIRSEKAKPDHDPNTRHCLYGLDADLIMLGLTSHEAHFSLLREEVRFGGKKTQRVCA PEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIERIIDDWILMGFLVGNDFIPHLPHL HINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSDFDREHFSEVFVDLKW FESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKDNLEDET EDDDLFETEFRQYKRTYYMTKMGVDVVSDDFLADQAACYVQAIQWILHYYYHGVQSWSWY YPYHYAPFLSDIHNISTLKIHFELGKPFKPFEQLLAVLPAASKNLLPACYQHLMTNEDSP IIEYYPPDFKTDLNGKQQEWEAVVLIPFIDEKRLLEAMETCNHSLKKEERKRNQHSECLM CWYDRDTEFIYPSPWPEKFPAIERCCTRYKIISLDAWRVDINKNKITRIDQKALYFCGFP TLKHIRHKFFLKKSGVQVFQQSSRGENMMLEILVDAESDELTVENVASSVLGKSVFVNWP HLEEARVVAVSDGETKFYLEEPPGTQKLYSGRTAPPSKVVHLGDKEQSNWAKEVQGISEH YLRRKGIIINETSAVVYAQLLTGRKYQINQNGEVRLEKQWSKQVVPFVYQTIVKDIRAFD SRFSNIKTLDDLFPLRSMVFMLGTPYYGCTGEVQDSGDVITEGRIRVIFSIPCEPNLDAL IQNQHKYSIKYNPGYVLASRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFN KKNEEVPGYTKKVGSEWMYSSAAEQLLAEYLERAPELFSYIAKNSQEDVFYEDDIWPGEN ENGAEKVQEIITWLKGHPVSTLSRSSCDLQILDAAIVEKIEEEVEKCKQRKNNKKVRVTV KPHLLYRPLEQQHGVIPDRDAEFCLFDRVVNVRENFSVPVGLRGTIIGIKGANREADVLF EVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLSHGSRSETGNQKLTAIVKPQPAVHQHSS SSSVSSGHLGALNHSPQSLFVPTQVPTKDDDEFCNIWQSLQGSGKMQYFQPTIQEKGAVL PQEISQVNQHHKSGFNDNSVKYQQRKHDPHRKFKEECKSPKAECWSQKMSNKQPNSGIEN FLASLNISKENEVQSSHHGEPPSEEHLSPQSFAMGTRMLKEILKIDGSNTVDHKNEIKQI ANEIPVSSNRRDEYGLPSQPKQNKKLASYMNKPHSANEYHNVQSMDNMCWPAPSQIPPVS TPVTELSRICSLVGMPQPDFSFLRMPQTMTVCQVKLSNGLLVHGPQCHSENEAKEKAALF ALQQLGSLGMNFPLPSQVFANYPSAVPPGTIPPAFPPPTGWDHYGSNYALGAANIMPSSS HLFGSMPWGPSVPVPGKPFHHTLYSGTMPMAGGIPGGVHNQFIPLQVTKKRVANKKNFEN KEAQSSQATPVQTSQPDSSNIVKVSPRESSSASLKSSPIAQPASSFQVETASQGHSISHH KSTPISSSRRKSRKLAVNFGVSKPSE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
5188
Start on reference sequence
68
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
