Detailed Vector Information: pPPL

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pPPL
Source:
None
Description:
ligation independent cloning (LIC) clone with pBR322 origin and Ampicillin Resistance and ROP and ORF frame 2 Tetracycline resistance and ORF frame 1 and pBRrevBam and T7 and T7 term primer and BseRI Stuffer and MBP signal sequence (M1-A26 of NP_290668) and RBS and MBP (K27-T392 of NP_290668) and HIS10 and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and T7 term and FC sequencing primer pET-PPL seq 2 R and AmpR; ampicillin resistance in bacterial;
Comments:
None
Publications:
None
Authors:
Debra T. Hansen
EvNO00813763
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AVAILABLE
No restriction
Special MTA: None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.