Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pCPD-pRL-CMV-ccdb
Source:
CPD
Description:
fluorescent marker assay, Gateway cloning, bacterial expression expression with Renilla Luciferase and pCEP Fwd Primer and ccdB and CMV and CAGF and CMV-F and Amp-R and lac promoter and attR1 and attR2 and T7 promoter and Chloramphenicol-R; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
Comments:
None
Publications:
None
Authors:
Shane Miersch
EvNO00813803
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
kanamycin
Growth Condition:
Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments:
None
Vector Name:
pCPD-pRL-CMV-ccdb
Synonyms:
Modified pRL-CMV
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
fluorescent marker assay, Gateway cloning, bacterial expression expression with Renilla Luciferase and pCEP Fwd Primer and ccdB and CMV and CAGF and CMV-F and Amp-R and lac promoter and attR1 and attR2 and T7 promoter and Chloramphenicol-R; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
Comments:
This vector is pRL-CMV modified by addition of a ccdB death cassette to make it compatible with the Gateway system.
Size (bp):
5870
Parent Vector:
Name:
pRL-CMV
Description: Reporter vector with Renilla luciferase from Promega with CMV and T7 promoters; ampicillin resistance in bacteria.
Properties:
Gateway, bacterial expression, fluorescent marker
Author Name:
Shane Miersch
Publications:
PMID:
25758251
Title: Antiviral antibody profiling by high-density protein arrays.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
fluorescent marker
Renilla Luciferase
Renilla luciferase gene
1912
2844
negative selection marker
ccdB
Ccd death gene present in 'empty vector' form only (replace with gene of interest)
1432
1737
primer
CMV-F
CMV_fwd_Primer
678
698
primer
pCEP Fwd Primer
pCEP_Fwd_Primer
722
741
promoter
Amp-R
Ampicillin resistance
4184
5044
promoter
T7 promoter
T7 Promoter
90
112
promoter
lac promoter
Lac promoter
346
369
promoter
CMV
CMV_immearly_promoter
169
721
promoter
CAGF
CAG enhancer
224
511
recombination site
attR1
AttR1 recombination site, used in Gateway cloning
197
321
recombination site
attR2
AttR2 recombination site, Gateway cloning
1778
1879
selectable marker
Chloramphenicol-R
Chloramphenical resistance gene
430
1089
