Gene:
Gene Symbol:
HPCAL4
Gene Name:
hippocalcin like 4
Original Clone ID:
FLH266158.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
Attempted to grow multiple times from plate and tube sources, but consistently unable to grow.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081534
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCGACCTTGCTGGACATTAAGAGCAGCGTGCTCAGGCAGGTGCAGGTGTGCCCGTCC
TTCCGCCGCAGGACTGAGCAGGACCCAGGGAGTGCCAGCGCCGACCCGCAGGAGCCTGCC
ACGGGGGCCTGGAAACCCGGGGATGGCGTGGAGTTCTTTGCCCACATGCGCCTCATGCTG
AAGAAGGGGGAAGGCAGACAGGGCTTGCCGTGCCTCGAGGCCTGGGGCTCTCCCTGCCGT
CAGAGCCCTCACCCTGGGACCCACACACAGGTCCCCCTGCGCAGTGGCTCGCCAGCACCC
CCGGAGCCTGTGGATCCCAGCCTCGGCCTGAGAGCCCTGGCCCCAGAGGAGGTGGAGATG
CTCTACGAGGAGGCCCTGTACACGGTGCTTTACCGCGCGGGTACCATGGGCCCTGACCAG
GTGGACGACGAGGAGGCCCTGCTCAGCTATCTCCAGCAGGTGTTTGGCACCAGCCTTGAG
GAGCACACTGAGGCCATCGAGCGAGTGAGGAAGGCCAAGGCCCCCACGTATGCCCTGAAA
GTCTCTGTCATGCGTGCCAAGAACCTTCTGGCCAAGGACCCCAACGGCTTCAGCGACCCA
TACTGCATGCTGGGCATCCTGCCTGCCTCGGACGCCACGCGGGAGCCCCGTGCACAGAAG
GAGCAGCGCTTCGGCTTCCGCAAGGGCAGCAAGCGCGGTGGACCCCTGCCTGCCAAGTGC
ATCCAGGTCACCGAGGTGAAGAGCAGCACCCTGAACCCCGTCTGGAAGGAGCACTTCCTC
TTCGAGATTGAGGATGTGAGCACGGACCAGCTGCACCTGGACATCTGGGATCATGACGAC
GATGTATCCCTGGTAGAAGCGTGCAGGAAGCTGAATGAAGTCATCGGCCTGAAGGGCATG
GGCAGGTACTTCAAACAGATCGTCAAGTCAGCCCGCGCAAACGGGACAGCAGGACCCACC
GAGGACCACACCGATGACTTCCTGGGGTGCCTCAACATACCTGTCCGGGAGGTGCCTGTG
GCTGGCGTCGACCGCTGGTTCAAGCTGGAGCCACGCTCCAGTGCCTCGCGTGTGCAGGGA
CACTGCCACCTGGTTCTCAAGCTGATCACTACGCAGAGGGATACGGCCATGAGCCAGCGC
GGGCGATCCGGCTTCCTGTCCCACCTGCTGCTGCTCAGCCATCTGCTGCGGTTGGAGCAC
TCAGCAGAGGAGCCCAACTCCAGCAGCTGGCGAGGAGAGCTCAGCACACCAGCCGCCACC
ATCCTCTGCCTGCACGGAGCCCAGAGCAACCTGTCACCCTTGCAGCTGGCCGTGCTGCAC
TGGCAGGTCAGCAGCCGCCACCATCAAACCTGCACGCTGGACTACAGCTACCTGCTGGGG
CTGCTGGAGGACATGCAGGCACACTGGGAAGAGGCTCCTTCACTGCCCCAGGAGCAGGAG
GAGAGCCTGGCTGATAGCCTTTCCGCCTTCTCTGAGTTCGGGCTGCAGCTGCTGCGCCAG
CTCCGAGACTACTTCCCTGCCACCAACAGCACCGCTGTCCACCGCCTGGAGCTGCTGCTG
AAGTGTCTGGGCAAGCTGCAGCTCTTCCAACCCTCCTTTGAGATCTGCCCCTTCGAGTCG
GAGCTGAACATGGACATTGCTGCGGCCCTGAAGAGAGGCAACCGTGAGTGGTACGACAGG
ATCCTGAATGCCAAGAGTCCCCGAGAGCAGCCAGGACCACAGCGCCTGCCTGGGCTGGTT
GTGCTGGCTGACGCCGTCTATGATGACCTTCAGTTCTGCTACAGTGTGTACGCCAGCCTC
TTCCACAGCATCCTCAATGTGGACGTCTTCACCCTGACCTTCCGGCAGCTGGAGCGTCTG
GTGGCTGAGGAGGCGTGGGTGCTGACGGAGGAGCTGAGCCCCAAGATGACCCTGGAGGTG
GCCTCGGGGCTCTTTGAGCTCTACCTGACCCTGGCTGACCTCCAGCGCTTCTGGGATAGC
ATCCCTGGCCGGGACAGCCGCTCTCTGGCCCTGGCTGGCATCCACGCCCCCTTCCTGCCT
GCTGTGAAGCTCTGGTTCCAAGTGCTGAGGGACCAGGCCAAGTGGAGGCTTCAGGGAGCC
GTGGACATGGACACGCTGGAGCCCGTGGACGCCTCCTCCAGGCACAGCAGCTCCGCAGCC
ACTGCTGGTCTCTGCCTCAGCCACATCCAGGAGTTGTGGGTGCGCCTGGCGTGGCCTGAC
CCTGCCCAGGCTCAGGGGCTGGGCACCCAGCTTGGCCAGGACGTGTGTGAGGCCACCCTC
TTCTATACGGAGCTGCTTCGGAAGAAGGTGGACACTCAGCCAGGGGCGGCCGGTGAAGCA
GTGAGCGAGGCGCTCTGCGTGGTCCTCAACAATGTGGAGCTCGTGCGCAAGGCTGCTGGG
CAGGCCTTGAAGGGCCTGGCATGGCCAGAGGGGGCCACGGGGCCCGAGGGGGTGCTCCCC
CGCCCTCTGCTCAGCTGCACACAGGCCCTGGACGATGATCTGCAACGGGAGGCCCACACG
GTGACAGCGCACCTGACCTCTAAGATGGTGGGCGACATCCGCAAGTATGTACAGCACATC
AGTCTCTCGCCTGACTCCATCCAGAACGATGAGGCCGTGGCCCCGCTCATGAAGTACCTG
GATGAGAAGCTGGCCCTGCTGAACGCCTCGCTGGTGAAGGGGAACCTGAGCAGGGTGCTG
GAGGCCCTGTGGGAGCTACTCCTCCAGGCCATTCTGCAGGCGCTGGGTGCAAACCGTGAC
GTCTCTGCTGATTTCTACAGCCGCTTCCATTTCACGCTGGAGGCCCTGGTCAGTTTTTTC
CACGCAGAGGGTCAGGGTTTGCCCCTGGAGAGCCTGAGGGATGGAAGCTACAAGAGGCTG
AAGGAGGAGCTGCGGCTGCACAAATGTTCCACCCGCGAGTGCATCGAGCAGTTCTACCTG
GACAAGCTCAAACAGAGGACCCTGGAGCAGAACCGGTTTGGACGCCTGAGCGTCCGTTGC
CATTACGAGGCGGCTGAGCAGCGGCTGGCCGTGGAGGTGCTGCACGCCGCGGACCTGCTC
CCCCTGGACGCCAACGGCTTAAGTGACCCCTTTGTGATCGTGGAGCTGGGCCCACCGCAT
CTCTTTCCACTGGTCCGCAGCCAGAGGACCCAGGTGAAGACCCGGACGCTGCACCCTGTA
TACGACGAACTCTTCTACTTTTCCGTGCCTGCCGAGGCGTGCCGCCGCCGCGCGGCCTGT
GTGTTGTTCACCGTCATGGACCACGACTGGCTGTCCACCAACGACTTCGCTGGGGAGGCG
GCCCTCGGCCTAGGTGGCGTCACTGGTGTCGCCCGGCCCCAGGTGGGCGGGGGTGCAAGG
GCTGGGCAGCCTGTCACCCTGCACCTGTGCCGGCCCAGAGCCCAGGTGAGATCTGCGCTG
AGGAGGCTGGAAGGCCGCACCAGCAAGGAGGCGCAGGAGTTCGTGAAGAAACTCAAGGAG
CTGGAGAAGTGCATGGAGGCGGACCCC
Protein sequence predicted based on nucleotide sequence
MSTLLDIKSSVLRQVQVCPSFRRRTEQDPGSASADPQEPATGAWKPGDGVEFFAHMRLML KKGEGRQGLPCLEAWGSPCRQSPHPGTHTQVPLRSGSPAPPEPVDPSLGLRALAPEEVEM LYEEALYTVLYRAGTMGPDQVDDEEALLSYLQQVFGTSLEEHTEAIERVRKAKAPTYALK VSVMRAKNLLAKDPNGFSDPYCMLGILPASDATREPRAQKEQRFGFRKGSKRGGPLPAKC IQVTEVKSSTLNPVWKEHFLFEIEDVSTDQLHLDIWDHDDDVSLVEACRKLNEVIGLKGM GRYFKQIVKSARANGTAGPTEDHTDDFLGCLNIPVREVPVAGVDRWFKLEPRSSASRVQG HCHLVLKLITTQRDTAMSQRGRSGFLSHLLLLSHLLRLEHSAEEPNSSSWRGELSTPAAT ILCLHGAQSNLSPLQLAVLHWQVSSRHHQTCTLDYSYLLGLLEDMQAHWEEAPSLPQEQE ESLADSLSAFSEFGLQLLRQLRDYFPATNSTAVHRLELLLKCLGKLQLFQPSFEICPFES ELNMDIAAALKRGNREWYDRILNAKSPREQPGPQRLPGLVVLADAVYDDLQFCYSVYASL FHSILNVDVFTLTFRQLERLVAEEAWVLTEELSPKMTLEVASGLFELYLTLADLQRFWDS IPGRDSRSLALAGIHAPFLPAVKLWFQVLRDQAKWRLQGAVDMDTLEPVDASSRHSSSAA TAGLCLSHIQELWVRLAWPDPAQAQGLGTQLGQDVCEATLFYTELLRKKVDTQPGAAGEA VSEALCVVLNNVELVRKAAGQALKGLAWPEGATGPEGVLPRPLLSCTQALDDDLQREAHT VTAHLTSKMVGDIRKYVQHISLSPDSIQNDEAVAPLMKYLDEKLALLNASLVKGNLSRVL EALWELLLQAILQALGANRDVSADFYSRFHFTLEALVSFFHAEGQGLPLESLRDGSYKRL KEELRLHKCSTRECIEQFYLDKLKQRTLEQNRFGRLSVRCHYEAAEQRLAVEVLHAADLL PLDANGLSDPFVIVELGPPHLFPLVRSQRTQVKTRTLHPVYDELFYFSVPAEACRRRAAC VLFTVMDHDWLSTNDFAGEAALGLGGVTGVARPQVGGGARAGQPVTLHLCRPRAQVRSAL RRLEGRTSKEAQEFVKKLKELEKCMEADP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
121
End on reference sequence
696
Mutations:
None
Discrepancy:
c345a
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2868
3677
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3820
4408
