RNF32 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  RNF32
Gene Name:  ring finger protein 32
Original Clone ID:
FLH266204.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
Attempted to grow multiple times from plate and tube sources, but consistently unable to grow.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081580
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UNAVAILABLE
No restriction
Special MTA: None
Insert sequence: 1086nts
Open reading frame: 1 to 1086

ATGTTAAAAAATAAGGGTCACTCATCTAAGAAAGATAACTTGGCAGTCAATGCAGTTGCT
TTACAAGATCACATTTTACATGATCTTCAACTTCGAAATCTTTCAGTTGCAGATCATTCT
AAGACACAAGTACAAAAGAAAGAGAACAAATCTCTAAAAAGAGATACAAAGGCAATAATA
GATACTGGACTTAAAAAAACTACACAGTGCCCCAAACTAGAAGACTCAGAAAAAGAATAT
GTTCTTGATCCCAAACCGCCGCCGTTGACTTTGGCACAGAAGTTGGGCCTCATTGGGCCT
CCACCACCTCCACTGTCATCAGATGAATGGGAGAAGGTGAAACAGCGCTCTCTCCTGCAA
GGGGACTCCGTGCAACCATGCCCCATCTGTAAAGAAGAATTCGAGCTTCGTCCTCAGGTG
CTGCTTTCATGCTCCCATGTGTTCCACAAAGCATGTCTTCAGGCTTTTGAAAAGTTCACA
AATAAGAAAACCTGTCCTCTCTGTAGAAAGAACCAGTATCAAACCCGAGTGATACACGAT
GGGGCCCGCCTGTTCAGAATCAAGTGTGTGACCAGAATCCAAGCCTACTGGAGAGGATGT
GTTGTTAGAAAGTGGTACAGAAACCTGAGGAAAACAGTACCTCCCACAGATGCCAAGTTA
AGAAAAAAATTCTTTGAAAAAAAGTTCACAGAAATCAGCCACCGCATCCTGTGCTCATAC
AACACCAACATTGAAGAGCTCTTTGCAGAAATCGATCAGTGCTTGGCCATAAATCGAAGT
GTTCTTCAGCAGTTGGAAGAAAAATGTGGCCATGAGATCACAGAAGAGGAATGGGAGAAA
ATCCAAGTGCAGGCTCTGCGCCGGGAGACCCACGAGTGCTCCATCTGCCTGGCCCCTCTC
TCCGCTGCTGGCGGTCAGCGCGTGGGTGCAGGCAGGCGTTCCAGAGAGATGGCCCTCCTG
TCCTGCTCACATGTGTTCCACCATGCGTGTCTGCTGGCACTAGAGGAGTTCTCCGTGGGA
GACAGGCCTCCTTTCCATGCCTGTCCTCTCTGCCGCTCCTGCTACCAGAAGAAGATTCTT
GAATGT

Protein sequence predicted based on nucleotide sequence

MLKNKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAII
DTGLKKTTQCPKLEDSEKEYVLDPKPPPLTLAQKLGLIGPPPPPLSSDEWEKVKQRSLLQ
GDSVQPCPICKEEFELRPQVLLSCSHVFHKACLQAFEKFTNKKTCPLCRKNQYQTRVIHD
GARLFRIKCVTRIQAYWRGCVVRKWYRNLRKTVPPTDAKLRKKFFEKKFTEISHRILCSY
NTNIEELFAEIDQCLAINRSVLQQLEEKCGHEITEEEWEKIQVQALRRETHECSICLAPL
SAAGGQRVGAGRRSREMALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKKIL
EC

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 119
End on reference sequence 1207
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable